Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Explain the Pour Plate Method
Isolated colonies can also be obtained by pour plate method. The method involves mixing of small volume of microbial suspension with molten nutrient agar at 45°C and pouring immediately into sterile petri plate. The microbial suspension should be diluted sufficiently to obtain separate colonies on plating. The pour plate method involves adding specified amount (0.1 ml or 1.0 ml) of the dilution to the sterile petri plate. Twenty to twenty five (20-25) ml of nutrient agar medium kept liquefied in a water bath at 45°-50° C is then added to the sterile plate and mixed with the dilution properly by gentle rotation of plate in a circular motion on the table top.
This results in uniform distribution of microorganisms. Once the agar has hardened, each cell is fixed in place and forms a distinct colony on incubation. Colonies appeared both within the nutrient agar, as well as, on the surface of agar plate. Microbial cells get fixed on solidification of agar and forms individual colonies on incubation. Colonies are present both on the agar surface and embedded in the nutrient medium. Colonies obtained at different dilutions are also highlighted in the Figure. Colonies growing on the surface can be used to inoculate fresh medium for pure cultures. The method can also be used for microbial cells enumeration in the original sample. We will practically try out this technique in the next practical. But here let us now highlight the advantages and disadvantages of this technique.
this is animal?
Q. From which features do osteichthyes and condrichthyes get these names? "Chondros" means cartilage, "ictis" means fish (both from the Greek) the name chondrichtians is for fi
Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4 Determine the Yersin
Q. What is the notochord? How is this structure formed? The notochord is a rodlike structure that outlines the supporting axis of the embryo and gives birth to the vertebral co
What do you mean by Advanced characters The traits, or characteristics, that an animal has that are not ancestral to the taxon. They appear later in the evolutionary history of
Q What are the most tremendous known representatives of the platyhelminth phylum? The most popular representatives of the platyhelminthes are worms that cause human diseases, l
Anemochory - Dispersal of Seeds Seeds that are dispersed by air currents are usually light or are provided with special structures to help them remain air-borne for long peri
Types of Surgery There may be two options for surgical correction. Urinary Diversion In this following procedures are performed: 1) Uretero sigrnoidostomy if there is
Define Food Sources of Calcium? Dairy products are of course the primary source of calcium followed by grains and pulses. Among the millets, ragi contains substantial amount of
Explain Cancer and Inhibition of Tumorigenesis? Polyphenols appear to play a preventive role although the molecular mechanisms of action and applicability to human cancer preve
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +1-415-670-9521
Phone: +1-415-670-9521
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd