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Explain the Most Probable Number (MPN) Techniques?
Different procedures and media may be used depending on the food tested and the information sought. Media which can be used include Brilliant green lactose bile broth, Eosin methylene blue agar, lauryl sulfate tryptose broth, violet red bile agar or lactose broth etc. Presumptive count of coliforms in food is determined using Lauryl Sulphate Tryptose (LST) broth and Violet Red Bile (VRB) agar.
Former helps in detection of microbes that produces gas from lactose (gas in Durham tube) while acid production is detected by VRB agar (purple red colonies surrounded with a precipitate). Additional testing is done by inoculating from the positive presumptive tests into Brilliant Green Lactose Bile (BGLB) broth for confirmation. Gas in Durham tube confirms the test. Samples from the positive confirmed test are then streaked on to Eosin Methylene Blue (EMB) agar for completing the test. Lactose fermenters produces the black or dark center colonies (Positive result). Black colonies with green sheen are probably of E. coli.
Explain the DNA structure in details? Structure : Each DNA molecule is a double stranded polymer, consisting of perhaps thousands or millions of linked nucleotides. The two
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Explain Requirements and Recommended Dietary Intake? A very small daily intake of vitamin C (10-15 mg/day for an adult) is required to avoid deficiency and stave off scurvy. Ho
why is it known as middle man
The cerebrum is clearly the most interesting part of the brain from the point of view of cognitive neuroscience. The cerebrum consists of highly symmetrical left and right hemisphe
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