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Explain the Most Probable Number (MPN) Techniques?
Different procedures and media may be used depending on the food tested and the information sought. Media which can be used include Brilliant green lactose bile broth, Eosin methylene blue agar, lauryl sulfate tryptose broth, violet red bile agar or lactose broth etc. Presumptive count of coliforms in food is determined using Lauryl Sulphate Tryptose (LST) broth and Violet Red Bile (VRB) agar.
Former helps in detection of microbes that produces gas from lactose (gas in Durham tube) while acid production is detected by VRB agar (purple red colonies surrounded with a precipitate). Additional testing is done by inoculating from the positive presumptive tests into Brilliant Green Lactose Bile (BGLB) broth for confirmation. Gas in Durham tube confirms the test. Samples from the positive confirmed test are then streaked on to Eosin Methylene Blue (EMB) agar for completing the test. Lactose fermenters produces the black or dark center colonies (Positive result). Black colonies with green sheen are probably of E. coli.
LEGAL RIGHTS OF PSYCHIATRIC PATIENTS: When a patient is admitted in psychiatric hospital he may be deprived of the freedom to leave the hospital, and also to maintain certai
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Phosphorus cycle is sedimentary cycle because it does not enter into atmosphere. It can be found on earth in water, soil and sediments. The phosphorus cycle is the biogeochemical c
Why do some physicians advise patients with a bladder infection to drink a lot of cranberry juice? Answer Cranberry juice contains chemicals that kill viruses and bacteria. The inc
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Explain the Procedure Estimation of the Amount of Bacteria? Now carry out the exercise following the steps enumerated herewith. 1. Prepare ten fold dilutions of E. coli cult
Is Neuron A synapses onto Neuron B Consider Neuron B in the frog central nervous system whose plasma membrane has a newly discovered ligand-gated ionotropic receptor, named the
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