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Explain the Most Probable Number (MPN) Techniques?
Different procedures and media may be used depending on the food tested and the information sought. Media which can be used include Brilliant green lactose bile broth, Eosin methylene blue agar, lauryl sulfate tryptose broth, violet red bile agar or lactose broth etc. Presumptive count of coliforms in food is determined using Lauryl Sulphate Tryptose (LST) broth and Violet Red Bile (VRB) agar.
Former helps in detection of microbes that produces gas from lactose (gas in Durham tube) while acid production is detected by VRB agar (purple red colonies surrounded with a precipitate). Additional testing is done by inoculating from the positive presumptive tests into Brilliant Green Lactose Bile (BGLB) broth for confirmation. Gas in Durham tube confirms the test. Samples from the positive confirmed test are then streaked on to Eosin Methylene Blue (EMB) agar for completing the test. Lactose fermenters produces the black or dark center colonies (Positive result). Black colonies with green sheen are probably of E. coli.
Determine the Food Sources of Vitamin A? Vitamin A or retinol performed vitamin A), as you may already know, is found only in foods of animal origin, such as milk, cheese, crea
How to measure Post Repair Prv/iv Pressure ? In the operating room, after coining of1 bypass and before removing cannulae the RV and LV pressures are measured. The pressures ar
What is the structure of the adult fern within which cells undergoing meiosis can be found? In these plants meiosis takes place within structures known as sorus (plural, sori),
the mass number is used to calculate the number of _________in one atom of an elemement. In order to calculate the number of neutrons you must subtract the _______from the ______.
Q. Explain States of matter Ans. Chemistry deals with the nature and composition of matter - anything that occupies space and has mass, and the changes which it undergoes. Matt
uses and advantages and disadvantages of immunolocalization
Hexokinase, that catalyzes the first irreversible step of glycolysis, is inhibited by glucose 6-phosphate. Thus when PFK is inhibited and fructose 6-phosphate builds up an
Q. Show the pH range of natural honey? The pH of natural honey ranges from 3.4 to 6.1. Acidity of honey is primarily due to presence of acids such as gluconic acid, pyruvic a
Prevention and control The organism is sensitive to penicillin, tetracycline and other broad-spectrum antibiotics. Newer generation drugs are being used for the treatment of affec
C o l l i ba c i ll o si s It is commonly seen in newly born farm animals and occurs in septicaemic and enteric collibacillosis forms. E tiology: The disea
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