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Explain the Most Probable Number (MPN) Techniques?
Different procedures and media may be used depending on the food tested and the information sought. Media which can be used include Brilliant green lactose bile broth, Eosin methylene blue agar, lauryl sulfate tryptose broth, violet red bile agar or lactose broth etc. Presumptive count of coliforms in food is determined using Lauryl Sulphate Tryptose (LST) broth and Violet Red Bile (VRB) agar.
Former helps in detection of microbes that produces gas from lactose (gas in Durham tube) while acid production is detected by VRB agar (purple red colonies surrounded with a precipitate). Additional testing is done by inoculating from the positive presumptive tests into Brilliant Green Lactose Bile (BGLB) broth for confirmation. Gas in Durham tube confirms the test. Samples from the positive confirmed test are then streaked on to Eosin Methylene Blue (EMB) agar for completing the test. Lactose fermenters produces the black or dark center colonies (Positive result). Black colonies with green sheen are probably of E. coli.
Explain about the Chytridiomycota - Fungi Chytridiomycota - Look at the Figure, which illustrates chytridiomycota. These are the simplest among true fungi and are commonly call
Nursing Management Propped up position. Monitor signs and symptoms and laboratory test results documenting myocarditis. Monitor adequacy of cardiac output and hemo
male sex organ
Mutations and genetic recombination (in sexually reproducing organisms) are major sources of variations in natural populations, In this section we will briefly mention the various
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Explain Wire loop and Tube Method 1. Use a 25-gauge dental injection needle, cut to remove beveled end 2. Both ends of-mm diameter steel ligature wire passed through the ne
Quaternary Structure (4 o Structure). Globular in structure. When two or more than two molecules of protein of tertiary structure are connected to each other through
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Q. What is Glucose Tolerance Test? Glucose Tolerance Test (GTT) is used for diagnosis of diabetes mellitus. In this test, blood sugar levels are measured after giving glucose
Q. Zonation in the lake biome? Precipitation that does not evaporate or penetrate the soil remains on the soil surface resulting in run off which flows down from the mountains
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