Explain the indole production test, Biology

Assignment Help:

Explain the Indole Production Test?

Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.


Related Discussions:- Explain the indole production test

Cell determination, Cell Determination Cell determination is a process...

Cell Determination Cell determination is a process through which portions of embryonic genome are selected for expression in particular embryonic cells. Determination to follo

Abandonment of the ammoniotelic excretion, Q Why after the passage of anima...

Q Why after the passage of animals from the aquatic to the terrestrial habitat does the abandonment of the ammoniotelic excretion occur? Ammonia is a highly toxic molecule if n

Nucleic acid hybridization, Nucleic acid thermodynamics is the learning of ...

Nucleic acid thermodynamics is the learning of how temperature affects the nucleic acid structure of dsDNA (double-stranded DNA). The melting temperature (Tm) is explained as the t

Psyiology, I need help with p-p, q-q and r-r intervals and heart rates

I need help with p-p, q-q and r-r intervals and heart rates

Explain monomeric enzymes, Monomeric enzymes Monomeric enzymes are thos...

Monomeric enzymes Monomeric enzymes are those which consist of only a single polypeptide chain, so they cannot be dissociated into smaller units. Very few monomeric enzymes are

Explain nicotinic acid, Nicotinic acid (Niacin) Niacin refers to both n...

Nicotinic acid (Niacin) Niacin refers to both nicotinic acid and its amide derivative. Nicotinic acid occurs as white or almost white crystals or as a crystalline powder of the

Define sex as a determinants of nutrient requirements, Define Sex as a dete...

Define Sex as a determinants of nutrient requirements? There are some differences in requirements between the two sexes. However, except for iron, these are apparent rather tha

Determine the nutritional and functional role of selenium, Minerals:- Sele...

Minerals:- Selenium  Food Source       Sea food, organ meats, cereals (levels vary depending on soil levels) Nutritional Functional role Essential nutrient: Keshan

Glycolysis, Glycolysis Oxidation of glucose is called as glycolysis.Glu...

Glycolysis Oxidation of glucose is called as glycolysis.Glucose is oxidized to either lactate or pyruvate. Under aerobic conditions and the dominant product within most tissues

Molecular bio, he recombinant plasmid extracted from the surviving colonies...

he recombinant plasmid extracted from the surviving colonies contains a gene called SUC2 that codes for convertase, an enzyme needed to metabolize sucrose. Mutations in the SNF gen

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd