Explain the indole production test, Biology

Assignment Help:

Explain the Indole Production Test?

Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.


Related Discussions:- Explain the indole production test

Can you define factor viii, Q. What is factor VIII? What is the genetic dis...

Q. What is factor VIII? What is the genetic disease in which this factor is absent? Factor VIII has the function of activating factor X that in its turn is necessary for the tr

Safety and First-Aid Procedures, How can a family prevent injuries from a f...

How can a family prevent injuries from a fire in the home

Define causes of iron deficiency anaemia, Define Causes of iron Deficiency ...

Define Causes of iron Deficiency Anaemia? Anaemia is a condition in which the blood cannot carry enough oxygen. This may be because there are fewer red blood cells than normal,

Mollusca, locomotary organ in mollusca

locomotary organ in mollusca

Explain the cladosporium - fungi and yeast, Explain the Cladosporium - Fung...

Explain the Cladosporium - Fungi and Yeast? Classification Kingdom - Mycetae Division - Amastigomycota Form - Class - Deauteromycetes Form - Order - Moniliales F

What will the responses look like expain, You have a neuron with a resting ...

You have a neuron with a resting potential of -55 mV, EK of -70 mV, and ENa of 50 mV. You decide to voltage clamp the neuron. Now, draw the membrane current over time. What will th

Importance of human milk for infant growth and development, Define the Impo...

Define the Importance of Human Milk for Infant Growth and Development? Let us now look at the value of human milk in promoting infant growth and development.  Success of lactat

What is the classification that divides orders, What is the classification ...

What is the classification that divides orders? Orders are divided into Families. The hierarchy of classification of living things most usually used is, from broadest to nar

Define thermal coagulation and film formation, Define Thermal Coagulation a...

Define Thermal Coagulation and Film Formation? 1. Thermal Coagulation and Film Formation: Concentrated soy proteins can be thermally coagulated on a flat metallic surface, such

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd