Explain the indole production test, Biology

Assignment Help:

Explain the Indole Production Test?

Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.


Related Discussions:- Explain the indole production test

Define association between inflammation and fever, Q. What is the associati...

Q. What is the association between inflammation and fever? In the tissue region where inflammation occurs prostaglandins, cytokines, bacterial toxins, interleukins and endothel

#titleclasses of fish.., what are the two main classes into which fish are ...

what are the two main classes into which fish are divided

Is the epithelium vascularized, Q. Is the epithelium vascularized? How do o...

Q. Is the epithelium vascularized? How do oxygen and nutrients reach the epithelium? Why is this feature an important evolutionary acquisition? Epithelia are not vascularized c

Heat energy is liberated during respiration, Some amount of energy is gener...

Some amount of energy is generatred from the oxidation of food materials is released as heat. This can be proved by germinating seeds. Aim: To show that heat is liberated during re

What are gametes, Q. What are gametes? Gametes are cells specialized in...

Q. What are gametes? Gametes are cells specialized in sexual reproduction and they contain half of the maximum number of chromosomes of the species and unite it with another ga

Explain use of rifaximin, Rifaximin (Xifaxan)  An anon-absorbed oral an...

Rifaximin (Xifaxan)  An anon-absorbed oral antibiotic, was about as effective as ciprofloxacin for treatment of travelers' diarrhea, mostly caused by E. coli. It is not effecti

Antibiotic resistance, Antibiotic resistance is the resistance conferred t...

Antibiotic resistance is the resistance conferred to the host the ability to survive a given antibiotic by the plasmids containing resistance genes. If such kind of plasmid is pre

What is atopic dermatitis, Atopic dermatitis is an inflammatory skin diseas...

Atopic dermatitis is an inflammatory skin disease. This disease is observed frequently in young children. There will be skin eruptions.

Developmental biology, a recent topic for my developmental biology assignm...

a recent topic for my developmental biology assignment

Which groups does not contain a carbonyl, Which of the following functional...

Which of the following functional groups does NOT contain a carbonyl? Select one: a. Aldehyde b. Carboxyl c. Phosphate d. Amide e. Organic ester

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd