Explain the indole production test, Biology

Assignment Help:

Explain the Indole Production Test?

Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.


Related Discussions:- Explain the indole production test

Male reproductive system - seminal vesicles, SEMINAL VESICLES (UTERUS MESCU...

SEMINAL VESICLES (UTERUS MESCULANIS) - Seminal vesicles are elongated, convoluted, musculo glandular sac about 4 cm long ( 9 cm if straightened). Lined by pseudostratified e

Work sheet, When the Balance Sheet column totals don't agree on the first a...

When the Balance Sheet column totals don't agree on the first attempt work backward through the process used in preparing the work sheet exclusively take the following steps until

Describe how micrornas are able to influence gene expression, 1. Transcript...

1. Transcriptional analyses of eukaryotic cells reveal widespread production of RNA. Using specific examples describe how: a) microRNAs are able to influence gene expression.

Defone economic consequences of malnutrition, Defone Economic consequences ...

Defone Economic consequences of malnutrition? Figure explains the economic consequences of malnutrition. You would note from the Figure that the economic productivity of the in

Mechanics of locomotion, Mechanics of Locomotion Among annelids, poly...

Mechanics of Locomotion Among annelids, polychaetes, hence more primitive than the other two groups, show a more complex mode of locomotion. Necessarily, the locomotion in po

Describe pharmacologic agents in amyl nitrite, Describe Pharmacologic Agent...

Describe Pharmacologic Agents in amyl nitrite ? Amyl Nitrite : It produces vasodilatation which initially results in marked fall in BP and in the next 30-60 seconds there is

What is the typical feature of the epithelia, Q. What is the typical featur...

Q. What is the typical feature of the epithelia? How different is it from the connective tissue? The typical feature of the epithelium is the almost absence or absence of space

Difference between micronutrients and macro, Q. What is the difference betw...

Q. What is the difference between micronutrients and macro? The classification criterion of nutrients into micronutrients and macro has no relation to the size of the molecul

Illustrate the molecular composition of hemoglobin, Q What is the molecular...

Q What is the molecular composition of hemoglobin? Does the functionality of hemoglobin as a protein depend upon its tertiary or upon its quaternary structure? Hemoglobi

What is deuterostome. explain in brief., What is Deuterostome. Explain in b...

What is Deuterostome. Explain in brief. Phyla, including the Chordata and Echinodermata which share common characteristics of blastopore-not forming the mouth, radial indetermi

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd