Explain the indole production test, Biology

Assignment Help:

Explain the Indole Production Test?

Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.


Related Discussions:- Explain the indole production test

What is the radiographic evaluation, Radiographic Evaluation An intraor...

Radiographic Evaluation An intraoral periapical radiograph with a paralleling technique should be made and observed to ensure absence of pathology in the peri-implant region an

Causes of diabetic ketoacidosis, Q. Causes of Diabetic Ketoacidosis? Th...

Q. Causes of Diabetic Ketoacidosis? The causes of Diabetic Ketoacidosis (DKA) are the following: - Missing of insulin injection - Infection - Trauma (injury) - Myoc

Factors responsible for food spoilage, Q. Can you explain Factors responsib...

Q. Can you explain Factors responsible for food spoilage? Spoilage, as you would realize, is quite a natural phenomenon. All foods undergo varying degrees of deterioration or s

How does the swim bladder of fishes work, Q. How does the swim bladder of f...

Q. How does the swim bladder of fishes work allowing fishes to control their depth under water? From Hydrostatics it is known that an object does not sink if its density is les

Psychopharmacology, PSYCHOPHARMACOLOGY: Psychotropic drugs are drugs u...

PSYCHOPHARMACOLOGY: Psychotropic drugs are drugs used for mentally ill patients for psychotic and neurotic condition.  1)  Antipsychotic or Neuroleptic Drugs and Anti-parki

Diagram and describe the signalling of toll, Diagram and describe the signa...

Diagram and describe the signalling of toll like receptors and the resulting cytokines.

Emulsion preparation, Em u l sio n preparation Frozen deboned meat,...

Em u l sio n preparation Frozen deboned meat, fat and by-products are tempered at 0° to 4° C for 12to 15 hr and cut into pieces and coarse minced by passing through 8, 10 o

Respiration in adult amphibians and fishes, Q. Do amphibians have direct de...

Q. Do amphibians have direct development? In amphibians the embryonic development is indirect there is a larval stage. Q. How different are the respiration in adult amphibi

What is congenital mitral stenosis, What is Congenital Mitral Stenosis ? ...

What is Congenital Mitral Stenosis ? The onset of symptoms or signs depends on severity of the stenosis, severe the stenosis earlier the presentation. With significant MS, the

Define function of the iron uptake by cells, Define Function of the Iron Up...

Define Function of the Iron Uptake by Cells? Iron has several vital functions in the body. It serves as a carrier of oxygen to the tissues from the lungs by red blood cell haem

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd