Explain the indole production test, Biology

Assignment Help:

Explain the Indole Production Test?

Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.


Related Discussions:- Explain the indole production test

Procedure for define percentage of reducing and total sugar, Procedure For ...

Procedure For Define Percentage of reducing and total sugar by fehling soxhlet? 1. Standard titration (Standardization of Fehling's solution) - Titrate 10 ml of Fehling's solut

Describe wide split s in details, Describe Wide Split S in details? It ...

Describe Wide Split S in details? It is a persistent split that widens on inspiration to at least 60 msec. May be due to delay in P, e.g. RBBB or due to early A, as occasionall

Male reproductive disorders due to infections, Male reproductive disorders ...

Male reproductive disorders due to infections Septicemic infections have a greater affinity for vesicular glands than other regions of the reproductive tract viz. prostate, am

Drinking & driving, DRINKING & DRIVING - It should not be mixed because...

DRINKING & DRIVING - It should not be mixed because alcohol - 1.       Impairs ability to judge distances. 2.       Reduces capacity to coordinate limb muscles. 3.

Determine the two different alleles, What is meant by saying that in relati...

What is meant by saying that in relation to a given trait conditioned by a gene with two different alleles the gametes are always "pure"? To say that gametes are pure means tha

Define the disorders due to the iodine deficiency, Define the Disorders due...

Define the Disorders due to the iodine deficiency? Mild goitre, i.e., a larger thyroid gland than normal. The mildest form of goitre ranges from those only detectable by to

Issues impacting health and development - inequity, Normal 0 fa...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Thermodynamics and energy, Thermodynamics and Energy 2 types of en...

Thermodynamics and Energy 2 types of energy (kinetic and potential) Work is the transfer of energy from one place to another Energy isn't lost, only converted è fi

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd