Explain the indole production test, Biology

Assignment Help:

Explain the Indole Production Test?

Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.


Related Discussions:- Explain the indole production test

DNA replication, What is cDNA and what is its function and mechanism of act...

What is cDNA and what is its function and mechanism of action?

List five advantages of dsme, Q. List five advantages of DSME? Followin...

Q. List five advantages of DSME? Following are the advantages of DSME. It enables the patient to 1. Accept the disease. 2. Gain knowledge about disease, its prevention

Blood salvage and bloodless open-heart surgery, Blood Salvage and Bloodless...

Blood Salvage and Bloodless Open-heart Surgery: At the time of cardio pulmonary bypass, cardiomony suckers suck blood from the chambers or the heart and pericardium back into the

What is the destination of the notochord, Q. What is the destination of the...

Q. What is the destination of the notochord in protochordates and in vertebrates? In vertebrates the notochord disappears and gives birth to the spinal column known as vertebra

Could this be accomplished by passive diffusion, In the human body, the pot...

In the human body, the potassium ion can pass easily through cell membranes, yet the potassium ion concentration is higher inside many cells than it is outside these cells. Could t

Zoonotic diseases-classification in terms of reservoir host, Classification...

Classification in terms of reservoir host 1.  Anthropozoonoses: Infections transmitted from lower vertebrate to man, e.g., rabies, plague, leptospirosis, etc. 2.   Zooanthro

Which cells produced in first stage of embryonic development, Q. What are t...

Q. What are the cells produced in the first stage of the embryonic development called? The cells that result from the cleavage (the first stage of the embryonic development) ar

Potential and excitation threshold for neurons, Q. How different are the co...

Q. How different are the concepts of action potential, resting potential and excitation threshold concerning neurons? Action potential is the maximum positive voltage level ach

What are the chronic complications of diabetes, Q. What are the Chronic com...

Q. What are the Chronic complications of diabetes? • Atherosclerosis: Degeneration of walls of the arteries due to fatty plaques - deposition on arterial walls. Diabetics ar

Rare species - wildlife, Rare Species - Wildlife These are those speci...

Rare Species - Wildlife These are those species whose numbers are few or they live in such small areas or in such unusual environments (endemics), that they could quickly disa

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd