Explain the indole production test, Biology

Assignment Help:

Explain the Indole Production Test?

Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.


Related Discussions:- Explain the indole production test

What are the feasible methods of stabilization, The feasible methods of sta...

The feasible methods of stabilization within the constraints of retaining natural status: Complex formation: Anthocyanin occurs naturally as complexes which are relatively st

What are the oligosaccharides and monosaccharides, Q. What are the oligosac...

Q. What are the oligosaccharides, monosaccharides and polysaccharides? Monosaccharides are simple molecules of carbohydrates that cannot be broken down into smaller molecules o

Respiratory gases -respiration, Respiratory Gases -Respiration Physio...

Respiratory Gases -Respiration Physiologically the most important gases are oxygen, carbon dioxide and nitrogen. Molecular oxygen comprises 21% of the atmosphere; carbon diox

The human abo blood type codominant alleles a and b, The human abo blood ty...

The human abo blood type codominant alleles a and b are dominant to O. A man with blood type B and women with blood type A could potentially have children with which of the followi

Egg - synergids, Egg - Synergids The three cells of the egg apparatus ...

Egg - Synergids The three cells of the egg apparatus are arranged in triangular fashion with the egg sharing a common wall with the two synergids and the central cell. In the

Display schematically and give all data, Through Nuclear transfer how Dolly...

Through Nuclear transfer how Dolly came into the picture. Show schematically and give all data.

Exercise and medications in bronchial asthma, Exercise Stress or em...

Exercise Stress or emotional upset  Medications Aspirin, non-steroidal anti-inflammatory drugs(NSAIDs), beta- blockers(including eye drops), cholinergic dr

State the active hyperemia, Which of the following is true for active hyper...

Which of the following is true for active hyperemia, a local control mechanism within the circulatory system? A. There will be a decrease in force developed by smooth muscles s

Explain wind pollinated flowers, Wind pollinated flowers are: 1. Small, ...

Wind pollinated flowers are: 1. Small, brightly coloured, producing large number of pollen grains 2. Small, producing large number of dry pollen grains 3. Large producing

Gram positive & gram negative bacteria , What is the difference among Gram ...

What is the difference among Gram positive & Gram negative bacteria based on cell content? Ans) The cell membrane is made if more of lipoproteins in gram negative compared to gr

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd