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Explain the Indole Production Test?
Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.
In what ways does over-grazing lead to soil erosion? Over-grazing leads to erosion because (i) the animals delete all the vegetation, so exposing the soil to heavy rain, (ii) t
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The vitamin niacin is a necessary component of NAD. Niacin can be consumed in food or manufactured in the body from tryptophan, an amino acid. How would a person's ability to break
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What is the excretory organ of lizard
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