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Explain the Indole Production Test?
Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.
Which factors are altered when cells are moved from an in vivo to an in vitro environment, and how do cells respond to these changes?
Importance of ecology
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How do adhesion and cohesion help plants move materials?
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Describe in detail - Nacreous layer. The innermost of the three layers of a mollusc shell. It is also referred to as mother-of-pearl, it comprises thin layers or calcium carbon
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