Explain the indole production test, Biology

Assignment Help:

Explain the Indole Production Test?

Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.


Related Discussions:- Explain the indole production test

Modern theory in biology, Modern Theory Naturalistic theory: Accordin...

Modern Theory Naturalistic theory: According to this theory Life originated upon our earth spontaneously from nonliving matter. But there are two significant points in this r

Define the meaning of vital signs, Define the meaning of Vital signs V...

Define the meaning of Vital signs Vital signs are measures of different physiological statistics, often taken by health professionals, in order to assess the most basic body f

#title.ehr, Why have biologists and bioinformaticians embraced the Web as a...

Why have biologists and bioinformaticians embraced the Web as a vehicle for disseminating data so quickly, whereas clinicians and clinical informaticians have been more hesitant to

Characteristics of the digestive system of earthworm, Q Which are the organ...

Q Which are the organs and characteristics of the digestive system of earthworms related to the kind of diet of these animals? Small organisms ingested together with soil parti

Marine extremophiles, how to make a assignment on recent research on marine...

how to make a assignment on recent research on marine microbiology

Explain the precautions for sub-culturing of a culture, Explain the Precaut...

Explain the Precautions for Sub-Culturing of a Culture 1. Sterilization of inoculating wire before and after the transfer is a must. Sterilized wire should not be kept on labor

Reproduction, briefly deccribe the eggs snd follicles

briefly deccribe the eggs snd follicles

Autoradiography, Autoradiography Autoradiography is a modification of t...

Autoradiography Autoradiography is a modification of the radioactive tracer technique. In this technique, the radioactivity is detected by a thin film of photographic emulsion

Estimation of protein by miscellaneous parameters include, Estimation of pr...

Estimation of protein by Miscellaneous parameters include? Miscellaneous parameters include Plasma amino acid levels Microbiological methods Chemical scoring

What do you mean by transgenic food, Q. What is transgenic food? The Tr...

Q. What is transgenic food? The Transgenic beings are animals, microorganisms and plants that contain recombinant DNA that is genes from other microorganisms, plants or animals

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd