Explain the indole production test, Biology

Assignment Help:

Explain the Indole Production Test?

Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.


Related Discussions:- Explain the indole production test

Gene pool, Natural selection can therefore mean differential reproduction a...

Natural selection can therefore mean differential reproduction among members of the sarne gene pool. We earlier said that selection would promote those alleles that give rise to ph

What are the valvular heart diseases, Q. What are the Valvular heart diseas...

Q. What are the Valvular heart diseases? Valvular heart disease is a common clinical problem. While rheumatic mitral valve disease seen more often in the underprivileged segmen

Chronic complications of diabetes, Q. Chronic complications of diabetes? ...

Q. Chronic complications of diabetes? These occur gradually when the diabetics do not monitor the blood sugar and they are careless about eating, exercise and the medications a

How body size and composition affecting the bmr, How Body size and composit...

How Body size and composition affecting the BMR? Basal and resting energy expenditures are related to body size, being most closely correlated with the size of the fat-free mas

Define precaution for estimation of vitamin c in lemon juice, Define precau...

Define precaution for estimation of vitamin c in lemon juice? 1. Rinse all glassware with 3% metaphosphoric acid before you begin your practical and subsequently each time you

Digestive system of class asteroidea, Digestive System of Class Asteroidea ...

Digestive System of Class Asteroidea Most asteroids are scavengers or carnivores and feed on snails, bivalves, polychaets, other echinoderms, fish, sponges, sea anemones and p

Phloem, why phloem made of living cells

why phloem made of living cells

Glucose rehydration therapy, The movement  of Na + and glucose  from the l...

The movement  of Na + and glucose  from the lumen  of the intestine  across  the epithelial  cell to the blood  sets up a dissimilarity  in osmotic  pressure  across  the cell. As

Is the embryonic development in birds indirect or direct, Q. Is the embryon...

Q. Is the embryonic development in birds indirect or direct? The embryonic development is direct there is no larval stage. Q. What are the predominating chemical compounds

What is linkage, What is linkage? Two genes are assumed to be under lin...

What is linkage? Two genes are assumed to be under linkage, or linked, when they reside in the same chromosome. For instance, the research of the human genome discovered tha

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd