Explain the indole production test, Biology

Assignment Help:

Explain the Indole Production Test?

Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.


Related Discussions:- Explain the indole production test

Functions of endoplasmic reticulum, FUNCTIONS OF E.R. (1 )      Mecha...

FUNCTIONS OF E.R. (1 )      Mechanical support - E.R. participates in formation of cytoskeleton with microfilaments, microtubules and intermediate filaments. (2 )

Explain about the deficiency of riboflavin, Explain about the Deficiency of...

Explain about the Deficiency of Riboflavin? Riboflavin deficiency results in the condition of hypo- or ariboflavinosis, with sore  throat, hyperaemia (condition in which the bl

Dietary recommendations in celiac disease, Q. Dietary Recommendations in ce...

Q. Dietary Recommendations in celiac disease? The only dietary treatment for celiac disease is to follow a gluten-free diet. For most such a diet improves symptoms, heals inte

Determine the concept of imitation in the learning process, Determine the c...

Determine the concept of  imitation in the learning process The concept of  imitation in the learning process is also a key developmental concept for infants and young children

Drug effects on food intake - cause sore or painful mouth, Define Drug effe...

Define Drug effects on food intake - Cause sore or painful Mouth? This is a common side effect of chemotherapy and can significantly affect food intake. Antineoplastic drugs, u

Explain the soft diet, Explain the soft diet The soft diet provides so...

Explain the soft diet The soft diet provides soft whole food that is lightly seasoned and moderately low in fibre. The foods have a soft  texture and are easy  to digest. Smal

Solutions, how many grams of table sugar would you need to dissolved in wat...

how many grams of table sugar would you need to dissolved in water to produce 0.75 liters of a 0:25 molar acqeous solution of table sugar?

Modern viral diseases, Important Modern viral diseases By  extensive us...

Important Modern viral diseases By  extensive use of vaccination and chemotherapy. Certain  serious viral diseases of the past. Like smallpox. Have been completely or largely e

Discuss about troponin receptor site, Skeletal muscle A. The myosin hea...

Skeletal muscle A. The myosin head is activated (energized) by the conversion of GTP (that is bound to myosin) to GDP and Pi (that are bound to myosin). B. Detachment of the

What are the main biological functions of water, What are the main biologic...

What are the main biological functions of water? Water is the basic solvent for chemical reactions of living beings; it is the major means of substance transportation in the ce

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd