Explain the indole production test, Biology

Assignment Help:

Explain the Indole Production Test?

Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.


Related Discussions:- Explain the indole production test

Dimentia, i dont know how to start

i dont know how to start

Developmental biology, a sea urchin egg is injected with an mRNA encoding t...

a sea urchin egg is injected with an mRNA encoding the cadherin protein fragment described above and an mRNA encoding the Pmar1 transcription factor. The embryo is allowed to devel

Infertility, what is the remedy of infertility

what is the remedy of infertility

Determine factors affecting zn absorption - micro minerals, Determine Facto...

Determine Factors affecting Zn Absorption - Micro Minerals? In the last unit you have studied that absorption of various minerals (bioavailability) is influenced by number of f

Microtubule assembly, Microtubule assembly: A) generally originates in the ...

Microtubule assembly: A) generally originates in the centrosome. B) occurs only during mitosis. C) occurs randomly throughout the cell. D) is regulated by myosin. E) is inhibited d

What units are the nucleic acids constituted, Q. Of what units are the nucl...

Q. Of what units are the nucleic acids constituted? What are chemical entities that compose that unit? The Nucleic acids are formed by sequences of nucleotides. Nucleotides

What factor contributed to the current variations, According to the theory ...

According to the theory of natural selection, what factor below contributed to the current variations in beak sizes of finch species on the Galapagos Islands? a. Finches dispersed

Illustrate the horizons in detail, Illustrate the Horizons in detail 'O...

Illustrate the Horizons in detail 'O'  is the  organic horizon  formed from t he organic litter derived from plants and animals and fresh or partially decomposed organic materi

Role of wound epidermis and apical epidermal cap, Role of Wound Epidermis a...

Role of Wound Epidermis and Apical Epidermal Cap Earlier described that amputation, the stump epidermal cells at the wound edge migrate over and quickly cover the wound to fro

Membrane that selects based on pore size, A transport process in which phys...

A transport process in which physical (hydrostatic) pressure drives part of a fluid through a membrane that selects based on pore size

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd