Explain the indole production test, Biology

Assignment Help:

Explain the Indole Production Test?

Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.


Related Discussions:- Explain the indole production test

Coagulation - blood collection , Coagulation - Blood Collection : Plasm...

Coagulation - Blood Collection : Plasma is used to minimize the time needed for coagulation so it is used is medical emergencies. There are many types of anticoagulants used

Surface water sources and charactersitics, Most of the surface water origin...

Most of the surface water originates directly from precipitation in the form of rainfall or snow. Ground water from springs also contributes to the flow of most of the streams. The

Blood pressure, An adequate level of pressure is needed in the arteries to ...

An adequate level of pressure is needed in the arteries to keep the blood flowing through the cardiovascular system. The chief determinant of arterial pressure is the volume of blo

What are the main human degenerative diseases, Q. What are the main human d...

Q. What are the main human degenerative diseases? The major human degenerative diseases are divided into three groups, neoplastic diseases and degenerative diseases of the nerv

What are biopolymers, Q. What are biopolymers? Polymers are macromolecu...

Q. What are biopolymers? Polymers are macromolecules made by the union of several smaller identical molecules, called monomers. Biopolymers are polymers present in the living b

Work injuries based on type of accidents, Work Injuries Based On Type of Ac...

Work Injuries Based On Type of Accidents Earlier we identified four kinds of injuries mainly based upon type of disability. This classification is needed for the purposes of c

Computed tomography, C omp uted tomography: Computed tomogr...

C omp uted tomography: Computed tomography (CT imaging) is also known as CAT (computed axial tomography) scanning and it is based on the X-ray principle. The CAT sc

Echocardiography, E c h o c a r d i og r a...

E c h o c a r d i og r ap h y : It is an exceptionally useful technique for diagnosing cardiovascular diseases, particularly in small an

Circulatory system transports gases, Q. What are the three kinds of respira...

Q. What are the three kinds of respiration in which the circulatory system transports gases? The circulatory system has an important role in branchial respiration, cutaneous re

What is peri-implantitis, Peri-Implantitis (Infective Process- Bacterial Or...

Peri-Implantitis (Infective Process- Bacterial Origin) Bacterial invasion of the peri-implant soft tissue results in its inflammation followed by rapid bone loss. Peri-Implanti

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd