Explain the indole production test, Biology

Assignment Help:

Explain the Indole Production Test?

Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.


Related Discussions:- Explain the indole production test

Describe about the term - neuropsychological assessment, Describe about the...

Describe about the term - Neuropsychological assessment Neuropsychological assessment has its roots in neurology, the branch of medicine that focuses on the nervous system and

Wet and dry thermal treatment, Wet and dry thermal treatment Wet therma...

Wet and dry thermal treatment Wet thermal treatment: it is based on exposure of shredded infectious waste to high temperature, high pressure steam. It is inappropriate for the

How vitamin e is directly related to vitamins a, How vitamin e is directly ...

How vitamin e is directly related to Vitamins A, D and K? A high intake of vitamin E interferes with the functions of other fat-soluble vitamins such as vitamin K absorpt

Controlling introduction of alien species, Controlling Introduction of Alie...

Controlling Introduction of Alien Species As you know the alien species can cause the existing population to decrease or even become extinct. The individuals of alien species

What is osmotic pressure, Q. What is Osmotic Pressure? Ans. Import...

Q. What is Osmotic Pressure? Ans. Important characteristic of a cell is 'osmosis'. You would recall reading about osmosis in the Applied Physiology Course in Unit 8. I

How is carbon dioxide released by cellular respiration, How is carbon dioxi...

How is carbon dioxide released by cellular respiration transported from the tissues to be eliminated through the lungs? In vertebrates almost 70% of the carbon dioxide is trans

Explain about genetic engineering, Genetic engineering has been successfull...

Genetic engineering has been successfully used for producing: 1. Transgenic mice for testing safety of polio vaccine before use in humans 2. transgenic models for studying ne

Gastropods - feeding and digestion in molluscs, Gastropods - Feeding and Di...

Gastropods - Feeding and Digestion in Molluscs In several gastropods the digestion is extracellular. Though, some herbivore gastropods like Crepidula are ciliary feeders and h

Water to flow within the xylem from the roots to the leaves, What are the f...

What are the forces that facilitate to make water to flow within the xylem from the roots to the leaves? Water enters the roots because of the root pressure and a water column

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd