Explain the indole production test, Biology

Assignment Help:

Explain the Indole Production Test?

Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.


Related Discussions:- Explain the indole production test

The signals for parturition originate from, The signals for parturition ori...

The signals for parturition originate from: 1. placenta only 2. placenta as well as fully developed foetus 3. oxytocin released from maternal pituitary 4. fully develop

Cleavage, what are the chemical changes during cleavage

what are the chemical changes during cleavage

Respiration, how can i calculate the energy released from burnt peanut

how can i calculate the energy released from burnt peanut

Name the food require during thyphoid, Food require during thyphoid So ...

Food require during thyphoid So remember the foods to be included are: Juices, soups, dal water, broths. Refined cereals and their products (e.g. maida, rava, bread, rice

What are the dietary guidelines for gastritis, Q. What are the dietary guid...

Q. What are the dietary guidelines for gastritis? Energy: Give adequate calories through frequent feedings or else proteins would be utilized for energy of repair work. P

Sonu, my question is what is phyletic lineage

my question is what is phyletic lineage

What are the zymogens, Q. What are the zymogens? proenzymes, or Zymogen...

Q. What are the zymogens? proenzymes, or Zymogens, are enzymes secreted in inactive form. Under some conditions a zymogen shifts to the active form of the enzyme. Zymogen secre

Explain some limitations of most probable number, Explain some Limitations ...

Explain some Limitations of Most Probable Number? 1. Large quantity of glassware is required. 2. Colony morphology cannot be determined. 3. It lacks precision.

Find out the degrees of freedom, -The expected phenotypic ratio of offsprin...

-The expected phenotypic ratio of offspring. -The expected genotypic ratio of offspring. -Calculate the expected values you would use on a chi-square test (use genotypic rati

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd