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Explain the Indole Production Test?
Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.
1. Name four experimental techniques to determine protein-protein interactions other than X-ray & NMR techniques. 2. How common are Coiled-coil motifs? Where are they found? Giv
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Flaws in Developmen of Human The human growth and development in spite of its complexity works perfectly most of the time. But while development goes awry it creates a giant
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CHROMOLIPID Formed by lipid & pigment, eg. carotene, xanthophylls. Lycopene present in tomato & red chilli. Carrot is rich in b-carotene, converted into vitamin A.
Define Historical example of Ecosystems Science? Mathematical and statistical tools are central to enhancing our understanding of large-scale systems and contain, for instance,
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Q. How is the chromosome region where the centromere is located called, and how chromosomes classified in the relation to the position of their centromere? The chromosome regio
Natural Gums These are derived from various plant as well as microbial sources and are divided into the following four basic categories: 1. Seaweed gums: Agar, Alginate,
Define The Names of Some Immemorial Plants? From the time immemorial plants have been known by some name, they are common, vernacular or local names given by different persons.
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