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Explain the Indole Production Test?
Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.
In today's global economy, goods flow from one country to another like never before, allowing the natural capital from one country to be utilized in many other countries. Some have
Alginate Alginates are produced by brown seaweeds (Phaeophyceae, mainly Laminaria). Alginates or algin, is a generic term for the salts and derivatives of alginic acid. Algin
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