Explain the indole production test, Biology

Assignment Help:

Explain the Indole Production Test?

Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.


Related Discussions:- Explain the indole production test

Vaccines, Ask quesWhat type of antigenic agents may constitute vaccines? ti...

Ask quesWhat type of antigenic agents may constitute vaccines? tion #Minimum 100 words accepted#

State the testing of ketones in urine, State the Testing of Ketones in Urin...

State the Testing of Ketones in Urine Ketones are also known as ketone bodies. Ketone bodies are catabolic products of free fatty acids and three ketone bodies that can be dete

Urinary system, Structures that l pyramidenaform the r

Structures that l pyramidenaform the r

Periodicity - qualitative characters, Periodicity (Phenology, Aspection) ...

Periodicity (Phenology, Aspection) It refers to the study of seasonal changes in the community, that is, the periodic phenomena of organisms in relation to their climate. Peri

How different is growth according to the biotic potential, How different is...

How different is the growth according to the biotic potential of a viral population from the growth according to the biotic potential of a bacterial population? The growth cur

Digestive system - mouth, MOUT H - It is as transverse slit. It is ...

MOUT H - It is as transverse slit. It is pseudo type i.e. not open directly into alimentary canal. Mouth is covered by upper & lower movable lips. Movement is due to arb

What are monosaccharides and polysaccharides, What are monosaccharides, oli...

What are monosaccharides, oligosaccharides and polysaccharides? Monosaccharides are normal molecules of carbohydrates that cannot be broken into smaller molecules of other carb

Describe how to prepare 200 ml of naoh, Describe how you would prepare 200 ...

Describe how you would prepare 200 ML of a 0.25 Msoluation of NaOH from a 3 M stock solution?

Non - pharmacological management, Great importance is being given to life s...

Great importance is being given to life style modifications because of definite benefits observed in patients who follow this. Life style changes have advantages not only in the co

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd