Explain the indole production test, Biology

Assignment Help:

Explain the Indole Production Test?

Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.


Related Discussions:- Explain the indole production test

What is temperate grasslands, What is Temperate Grasslands? Grasslands ...

What is Temperate Grasslands? Grasslands are known by their common names in different regions of the world, such as the plains or prairies in North America, the steppe in Asia,

Etiologic factor of ulcerative colitis, Q. Etiologic factor of ulcerative c...

Q. Etiologic factor of ulcerative colitis? No single etiologic factor has been identified although genetic auto-immune factors are thought to be involved. Although exacerbation

Explain herbals of brunfels, Explain Herbals of Brunfels? Between the y...

Explain Herbals of Brunfels? Between the years 1530 and 1536 Otto Brunfelsius (Brunfels) (1463-1534) published his 'Herbal' which consisted of descriptions of a large number of

Digestive enzymes, Digestive Enzymes Now let us consider the general p...

Digestive Enzymes Now let us consider the general principles of digestion that are applicable t6all animals. We will start with the digestive enzymes that breakdown the large

Explain the term chemical score, Explain the term Chemical Score? Since...

Explain the term Chemical Score? Since egg proteins contain all essential amino acids in adequate amounts and possess the highest nutritive value among dietary proteins, Block

Uptake of ammonia, Uptake of Ammonia Ammonia (NH 3 ) diffuses freely a...

Uptake of Ammonia Ammonia (NH 3 ) diffuses freely across biological membranes according to its concentration gradient. However, ammonium (NH + 4 ) ion requires a specific tra

Possible errors in drug administration, POSSIBLE ERRORS IN DRUG ADMINISTRAT...

POSSIBLE ERRORS IN DRUG ADMINISTRATION There can be various reasons for error in administering drugs: i) Poor communication of intention by the prescriber. ii) Failur

State in detail about dark adaptation, State in detail about Dark Adaptatio...

State in detail about Dark Adaptation Dark adaptation is a phenomenon by which retina and pupil react to decreased illumination. Test is performed using Goldmann-Weeke's adapto

Define physiologic functions of iodine, Define Physiologic Functions of Iod...

Define Physiologic Functions of Iodine? Thyroid hormone performs multiple functions as regulator of cell activity and growth. The hormone has crucial metabolic roles in the foe

What is herbicide tolerant crops in ecological, Herbicide tolerant crops (H...

Herbicide tolerant crops (HTC) Increased use of the herbicides plant is tolerant to (describes ecological impact eg toxic residues in soil, runoff into waterways) Ther

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd