Explain the indole production test, Biology

Assignment Help:

Explain the Indole Production Test?

Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.


Related Discussions:- Explain the indole production test

When the greatest buffering capacity of weak acids, The greatest buffering ...

The greatest buffering capacity of weak acids is when- Select one: a. They are almost completely dissociated b. They are almost completely undissociated c. They are nea

Nutrition, what is the mode of nutrition in tape worm

what is the mode of nutrition in tape worm

Digestive system - salivary glands, SALI V A R Y GLAND - These are ...

SALI V A R Y GLAND - These are compound - Alveolar, tubular glands made up of columnar / cuboidal epithelium. Saliva is manufactured in them. In mammals these are pai

Explain adverse effects of adefovir dipivoxil, Adverse Effects of Adefovir ...

Adverse Effects of Adefovir dipivoxil  Doses of adefovir used for HBV are generally well tolerated but may be associated with asthenia, headache, diarrhea and abdominal pain. H

Explain cell membrane and plasma membrane, Explain cell membrane? The...

Explain cell membrane? The Cell Membrane :  The cell membrane, or plasma membrane, separates the cell from neighboring cells or from the external environment. Both prokaryot

Define the clinical success for the root canal treatment, Define the Clinic...

Define the Clinical success for the Root Canal Treatment a) Absence of pain and swelling. b) Disappearance of sinus tract. c) No evidence of soft tissue destruction, incl

Explain acid butt - carbohydrate utilization pattern test, Acid butt - carb...

Acid butt - carbohydrate utilization pattern test ? Acid butt (yellow) and acid slant (yellow) with or without gas production – Glucose along with lactose and/or sucrose, is deg

Haccp - what is validation, HACCP - What is Validation Validation   : ...

HACCP - What is Validation Validation   :  That element of verification focused on collecting and evaluating scientific and technical information to determine  if  the HACCP

How does the amoeboid movement occur, Q How does the amoeboid movement occu...

Q How does the amoeboid movement occur and what are examples of beings and cells that use such movements for locomotion? Amoeboid movements are created by cytoplasmic movements

Phanerophytes - classes of life form, Phanerophytes - Classes of Life Form ...

Phanerophytes - Classes of Life Form The perennating buds in this case are present on erect, negatively geotropic shoots, much above the ground. These buds are naked or least

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd