Explain the indole production test, Biology

Assignment Help:

Explain the Indole Production Test?

Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.


Related Discussions:- Explain the indole production test

Classification and features of echinodermata, Classification with its Justi...

Classification with its Justification Kingdom Animalia Animals, multi-cellular organisms with cells that lack a cell wall, many capable of movement or movement of some o

Define iron status of the individual, Define Iron Status of the Individual ...

Define Iron Status of the Individual Lastly, iron status of the individual is a floury determinant of how much iron is absorbed. On a mixed diet with some haem iron, the overal

Identify the enzymes utilization in cheese production, Cheese Production ...

Cheese Production Cheese production involves the conversion of the milk protein, k-casein to paracasein by a defined, limited hydrolysis, catalysed by chymosin (rennin). In the

Explain the metabolic aberrations of underweight, Explain the Metabolic Abe...

Explain the Metabolic Aberrations of Underweight? When energy intake falls below the minimal requirements, the body responds with an orderly physiologic adaptation involving th

Define atherosclerosis, Q. Explain Atherosclerosis? Atherosclerosis is ...

Q. Explain Atherosclerosis? Atherosclerosis is one of the most important causes of morbidity and mortality in developed as well as in developing countries. Atherogenesis occurs

Explain clear liquid diet, Explain Clear Liquid Diet Clear Liquid Die...

Explain Clear Liquid Diet Clear Liquid Diet:  It is composed  of  foods with  low residue content which  help  to minimize  the  load of food needing digestion  in  the  int

Why heartwood differs from sapwood, Heartwood differs from sapwood in: 1...

Heartwood differs from sapwood in: 1. Presence of rays and fibres 2. Absence of vessels and parenchyma 3. Having dead and non-conducting elements 4. Being susceptible t

Define observation or inference for picric acid test, Define observation or...

Define observation or inference for picric acid test? 1. A mahogany red colour will be seen. The mahogany red colour indicates the presence of reducing sugar. All monosaccharid

Homework, #questiocan you help in a biology question?.

#questiocan you help in a biology question?.

Explain boiling point - properties of solution, Boiling Point There are...

Boiling Point There are certain properties of solutions  which are directly connected with vapour pressure and one of it is boiling point.  You must have observed that water bo

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd