Explain the indole production test, Biology

Assignment Help:

Explain the Indole Production Test?

Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.


Related Discussions:- Explain the indole production test

Determine the various neurobehavioural characteristics, Determine the vario...

Determine the various neurobehavioural characteristics Contemporary views of brain function tend to conceptualise localisation in interaction with a number of developmental and

Electro magnetic radiation, Electro magnetic radiation Light is one ...

Electro magnetic radiation Light is one form of energy. The light rays consist of tiny particles called photons. The energy carried by photon is called quantum. Light

What is the mendel’s second law, What is the Mendel's second law? The M...

What is the Mendel's second law? The Mendel's second law postulates that two or more different traits are also conditioned by two or more pair of different factors that every i

Viruses, Are viruses cellular organisms?

Are viruses cellular organisms?

Define precautions for acid fast staining, Define Precautions for Acid Fast...

Define Precautions for Acid Fast Staining? 1. Do not allow carbol fuchsin to dry on heating. 2. Maintain aseptic conditions. 3. Decolourization should be done till no red

Determine the foos sources of cyanocobalamin, Determine the Foos Sources of...

Determine the Foos Sources of cyanocobalamin? Vitamin B I2 is unique among vitamins in the sense that it is mostly found in foods of animal origin but is not generally present

What are the cardiac muscles, What are the Cardiac muscles We know tha...

What are the Cardiac muscles We know that the heart muscles (cardiac muscles) are not under the control of  mind. The day we are born, it starts its activity and the day peopl

Social determinants of health - food, Social Determinants of Health - Food ...

Social Determinants of Health - Food The twin paradox of food i.e., research showing excessive intake can lead to a variety of diseases whilst at the same time food poverty be

Nutrition intervention, Nutrition intervention Nutrition intervention, ...

Nutrition intervention Nutrition intervention, the third step of  the nutrition care process, is a specific set of activities and associated materials used  to address the prob

Define nutrition counseling - management of eating disorders, Define Nutrit...

Define Nutrition Counseling - Management of Eating Disorders? Nutrition counseling can be used to accomplish a variety of goals, such as reducing behaviours related to the eati

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd