Explain the indole production test, Biology

Assignment Help:

Explain the Indole Production Test?

Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.


Related Discussions:- Explain the indole production test

Explain about individual consciousness, Q. Explain about Individual conscio...

Q. Explain about Individual consciousness? The physical brain is not a source of experience as some current neuroscientists now mistakenly believe. While areas of the brain are

How is extracellular digestion related to cellular, How is extracellular di...

How is extracellular digestion related to cellular and tissue specialization? Several of specialized cells and tissues appeared with extracellular digestion to give enzymes and

Newcastle disease / ranikhet disease (nd/rd), N e wcastle disease / Ranik...

N e wcastle disease / Ranikhet disease (ND/RD) This disease is caused by Newcastle disease virus (NDV) belonging to the genus Paramyxovirus of the family Paramyxoviridae. Alo

Northern blot, Northern blot is a technique for analyzing mixtures of RNA ...

Northern blot is a technique for analyzing mixtures of RNA by transfer of size-separated RNA fragments to a synthetic membrane, the presence and rough size of one particular type

How does hypophysis- corpus luteum negative feedback work, Q. How does the ...

Q. How does the hypophysis- corpus luteum negative feedback work? What is the name given to the atrophied corpus luteum after this feedback process? After ovulation the estroge

Illustrate the tyndallisation, Q. Illustrate the tyndallisation? John T...

Q. Illustrate the tyndallisation? John Tyndall devised a process of sterilisation by steaming for a few minutes at 100 o C on 3-4 successive operations separated by 24 hour in

Illustrate the structure of the central nervous system, Q. What is the stru...

Q. What is the structure of the central nervous system that regulates pulmonary respiration? The reaction is catalyzed by the enzyme carbonic anhydrase present in red blood cel

What are good pharmacovigilance process, Question 1: What are Good Phar...

Question 1: What are Good Pharmacovigilance Process and Good Post-Marketing Surveillance Process? How do they differ from each other? Define and illustrate briefly Good Post

Complications in gastric ulcers, Q. Complications in Gastric ulcers? Pe...

Q. Complications in Gastric ulcers? Peptic ulcers if not managed properly can result in serious consequences which can even be life threatening. The complications involved in

Nutritional management for constipation, Q. Nutritional management for cons...

Q. Nutritional management for constipation? Calories, proteins, carbohydrates and fat the requirements would be the same as the RDA for a particular age, sex, occupation of t

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd