Explain the indole production test, Biology

Assignment Help:

Explain the Indole Production Test?

Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.


Related Discussions:- Explain the indole production test

Explain about the management of food allergies, Normal 0 false ...

Normal 0 false false false EN-IN X-NONE X-NONE

Explain about the lactation process, Explain about the Lactation Process? ...

Explain about the Lactation Process? Lactation is a physiologic process which has profound relevance for both the mother and the newborn. It is the period following pregnancy w

How sugar is used in meringues, How sugar is used in Meringues? Egg whi...

How sugar is used in Meringues? Egg whites beaten for a meringue hold air bubbles as the mechanical action of the beaters partly coagulates the egg protein. Sugars make the pro

Determine a testable hypothesis, A friend of yours suggests that the origin...

A friend of yours suggests that the origin of life on earth is extraterrestrial in origin (an idea known as panspermia). Can you propose a testable hypothesis to determine if this

Can you explain pentoses, Q. What are the pentoses? What are the vital role...

Q. What are the pentoses? What are the vital roles of pentoses in DNA and RNA molecules? Pentoses are carbohydrates whole of five carbons. The DNA molecule is made of a sequ

Determine the types of cell, Determine the types of Cell Based OII the ...

Determine the types of Cell Based OII the degree of complexity of the cell organisation, two types of cells, namely, prokaryotes and eukaryotes, are recognised. The prokaryotic

Whcih animals are triploblastic, Which one of the following kinds of animal...

Which one of the following kinds of animals are triploblastic? 1. Flat worms 2. Sponges 3. Ctenophores 4. Corals Flat worms

Define about blood pressure control, Normal 0 false false f...

Normal 0 false false false EN-IN X-NONE X-NONE

Peste des petits ruminants, P e s t e des petits ruminants It is al...

P e s t e des petits ruminants It is also known as goat plague or kata resulting in high fever, diarrhoea and respiratory distress. In India, the disease was reported in th

Is it necessary for blood to have less or more hemoglobin, Q. In high altit...

Q. In high altitudes is it necessary for the blood to have less or more hemoglobin? In high altitudes the oxygen concentration and air is rarefied is lower than in low altitude

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd