Explain the indole production test, Biology

Assignment Help:

Explain the Indole Production Test?

Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.


Related Discussions:- Explain the indole production test

Define briefly about the seed proteins, Define briefly about the Seed Prote...

Define briefly about the Seed Proteins? Although a large number of plants produce seeds having protein contents in excess of 15%, only a few are utilized for food, eg. soybean,

How are grasslands classified, How are grasslands classified? Grasslan...

How are grasslands classified? Grasslands might be classified into steppes and savannahs. In the steppes the prevailing vegetation is grass, as in the pampas of South America

Prosthetic valve endocarditis, The risk of PVE is greatest during the initi...

The risk of PVE is greatest during the initial 6 months after valve surgery (particularly during the initial 5 to 6 weeks) and thereafter declines to a lower but persistent risk (0

Experimenting, what are some examples of eperimenting palants?

what are some examples of eperimenting palants?

Mycotoxicoses, Mycotoxicoses Mycotoxicoses cause heavy economic loss d...

Mycotoxicoses Mycotoxicoses cause heavy economic loss due to high morbidity and consequent production loss. Low grade mortality is also recorded. The main toxins involved are

To study if bacteria grow better where it is warm or cold, To study if bact...

To study if bacteria grow better where it is warm or cold Again inoculate two sterile dishes. Label one 'warm'  and the other 'cold'. Place the 1st dish in a dark warm place an

What are the kinds of reproduction that occur in fungi, Q What are the kind...

Q What are the kinds of reproduction that occur in fungi? In fungi there is sexual and asexual reproduction. Fungi reproduce asexually by fragmentation, sporulation and gemmati

#to work as expert, Ask question #process for work as expert#

Ask question #process for work as expert#

What are important terms in accommodation and convergence, What are importa...

What are important terms in accommodation and convergence? Accommodation and Convergence In this section some important terms are included: a. How a person is able to foc

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd