Explain the indole production test, Biology

Assignment Help:

Explain the Indole Production Test?

Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.


Related Discussions:- Explain the indole production test

Foliose lichen stage - xerarch, Foliose Lichen Stage - Xerarch As ment...

Foliose Lichen Stage - Xerarch As mentioned earlier, the weathering of the rocks and the decaying of the crustose lichens results in the formation of soil on the otherwise bar

Nutrition, list and explain the modes of nutrition

list and explain the modes of nutrition

Explain about the enteral feeding in elderly, Explain about the Enteral fee...

Explain about the Enteral feeding in elderly? Enteral, as you may already be aware, is defined as provision of nutrition support through the GI tract or by accessing the gut. Th

.development, what is the function of the larval stage in the development o...

what is the function of the larval stage in the development of the young of five named animals

Why do organisms live in certain places, Why do organisms live in certain p...

Why do organisms live in certain places? Think of that, the temperature dissimilarity in the desert is huge. So in order to survive, the cactus plant decreases heat gain and he

Vacuoles, VACUOLES They are of 4 types - 1.       Sap Vacuole - ...

VACUOLES They are of 4 types - 1.       Sap Vacuole - Outer membrane of sap vacuole is called T onoplast . Sap vacuole contains amino sugars, carotenoids, proteins, m

What are the applications of nicotinic acid, What are the Applications of N...

What are the Applications of Nicotinic acid Nicotinic acid is mainly used in the vitamin fortification of flour, macaroni and noodle products. Independent of its vitamin effic

Poo, BBBBBBBBBBBBBiiiiiiiiiiittttttttttcccccccchhhhhhhhhhhhhhh ass cunt.

BBBBBBBBBBBBBiiiiiiiiiiittttttttttcccccccchhhhhhhhhhhhhhh ass cunt.

Explain the use of benzoates acid, Q. Explain the use of Benzoates acid? ...

Q. Explain the use of Benzoates acid? Benzoic acid, sodium benzoate and the parahydroxy esters (parabens) are used as preservatives. Benzoic acid and its sodium salt, sodium be

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd