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Explain the Indole Production Test?
Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.
Discuss the refractive and response to wound functions of cornea. Refractive Function: Cornea, through its interaction with the tear film, forms a smooth refractive functi
Structure of human spermatozoon: Nature: Human sperms are minute, microscopic and motile. Structure: Each sperm have an oval shaped head piece, a neck, a middle piece and
how nematodes adapted to their mode of feedings
High Energy Compounds Primary need of the animal body from the nutrients is energy. Free energy is the term used to denote the energy available to do useful work. Anabolic or synth
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Vitamins Vitamins, as you may already know, derive its name from the word 'Vital Amine'. These are the organic compounds required for normal metabolism, growth and developme
Q: What are disaccharides? Although theoretically disaccharides could be made up of two trioses, tetroses, pentoses but all naturally occurring disaccharides contain hexose mol
Q. Monounsaturated fatty acids required for dyslipidemia? Polyunsaturated fatty acids also have a cholesterol decreasing effect. Omega 3 fatty acids are excellent for the heart
Define Reagents for Determination of the Saponification Number of Fats? 0.5 N alcoholic KOH 0.5 N HCI Solid sodium carbonate 1% alcoholic solution of methyl orange
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