Explain the indole production test, Biology

Assignment Help:

Explain the Indole Production Test?

Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.


Related Discussions:- Explain the indole production test

What do you know by hemodynamic responses, Q. What do you know by Hemodynam...

Q. What do you know by Hemodynamic Responses? Reduced exercise capacity roughly reflects impaired LV function, and may contribute its prognostic value to this association. Comp

Explain about chthalamus, Explain about chthalamus The realised niche o...

Explain about chthalamus The realised niche of Chthalamus is reduced from its fundamental niche and it is limited to the upper tidal zone Lower  limits Out-competed

Discuss about the halstead reitan battery, Discuss about the Halstead Reita...

Discuss about the Halstead Reitan battery The Halstead Reitan battery continues to be widely used as a clinical and research procedure. Numerous investigators use it in their r

Complications causes by gastro oesophageal reflux disease, Q. Complications...

Q. Complications Causes by gastro oesophageal reflux disease? Apart from the symptoms, which you have just read above, there are a few extra oesophageal manifestations of GERD.

Elaborate the internal vascular circulation of an eye, Elaborate the intern...

Elaborate the internal vascular circulation of an eye. Retinal vessels are derived from central retinal artery and are distributed within the inner two-third of retina. Outer o

Bio geographical evidence of evolution, BIO GEOGRAPHICAL EVIDENCE- T...

BIO GEOGRAPHICAL EVIDENCE- The patterns of distribution of animals and plants in different parts of the globe are termed as biogeography. It is believed that around carbo

Tanco beans plant, what are the origin, morphology, active constituents, us...

what are the origin, morphology, active constituents, uses and market preparation of tanco beans ?

Can you explain embryonic sac, Q. What is embryonic sac? Which are the cell...

Q. What is embryonic sac? Which are the cells that form the embryonic sac? What are their ploidies? The embryonic sac is female gametophyte of angiosperms. The embryonic sac

Explain haemophilus influenzae, Haemophilus influenzae type b (Hib) Hi...

Haemophilus influenzae type b (Hib) Hib is endemic worldwide. Previously unvaccinated infants

Autotrophic and heterotrophic succession, Autotrophic and Heterotrophic Suc...

Autotrophic and Heterotrophic Succession The succession where initially the green plants are much greater in quantity than the animals, is known as autotrophic succession. Su

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd