Explain the indole production test, Biology

Assignment Help:

Explain the Indole Production Test?

Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.


Related Discussions:- Explain the indole production test

Nursing assessment - hypospadias, Nursing Assessment Hypospadias can b...

Nursing Assessment Hypospadias can be observed by nurse or parents at birth. The child presents with abnormal pattern of voiding and presence of chordae. The stream of ur

Odyssey, how does odysseus get the dead to talk to him?

how does odysseus get the dead to talk to him?

What is the spinal cord, What is the spinal cord? Of which elements is the ...

What is the spinal cord? Of which elements is the spinal cord constituted? The spinal cord is the dorsal neural cord of vertebrates. It is the kind of the central nervous syste

Electric charge between neuron plasma membrane, Q. Which is the normal sign...

Q. Which is the normal sign of the electric charge between the two sides of the neuron plasma membrane? What is the potential difference (voltage) generated between these two sides

Heparinisation-procedures of oxygenators, Heparinisation :  The patient sh...

Heparinisation :  The patient should be fully heparinised before the start of cardio pulmonary bypass. Baseline activated clotting time is measured (ACT). 3 mg (300 units) per k

Serotonin - enhancement of memory, Q. Serotonin - Enhancement of memory? ...

Q. Serotonin - Enhancement of memory? Increased level of serotonin is implicated in the enhancement of memory. It plays a significant role in classical conditioning wherein sti

Define traditional methods of food processing, Define Traditional Methods o...

Define Traditional Methods of Food Processing? Because food is so important to survival and most foods remain edible for only a brief period of time, people as the initial ages

Explain phylum rhodophyta, Phylum Rhodophyta (Red algae) 1) The photosy...

Phylum Rhodophyta (Red algae) 1) The photosynthetic pigments include red pigments (phycoerythrin) and blue pigment (phycocyanin) apart from chlorophyll, of which red pigment pr

Class of coclentrata, ????? # 100 ??????????? #Minimum ?????? ?????

????? # 100 ??????????? #Minimum ?????? ?????

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd