Explain the indole production test, Biology

Assignment Help:

Explain the Indole Production Test?

Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.


Related Discussions:- Explain the indole production test

Parasite and host during incubation period, Parasite and host during incuba...

Parasite and host during incubation period Incubation period is the second stage in the process of a disease. It is the time between the day of infection to the appearanc

Can we prevent ketoacidosis, Q. Can we prevent ketoacidosis? Normally, ...

Q. Can we prevent ketoacidosis? Normally, while maintaining a good sugar control, there is a very little danger of ketoacidosis. One should test for ketones under the following

Gametes and genotypes, what type of gametes would be produced from genotype...

what type of gametes would be produced from genotype AA, Bb, AABb and someone who is heterozygous for 3 traits

Give the biological effects of aflatoxin, Q. Give the biological effects of...

Q. Give the biological effects of aflatoxin and List the various natural toxins present in food? Biological effects of aflatoxin include hepatotoxicosis, carcinogenesis and liv

<-- that guy, thy does he look too excited in the illistration?

thy does he look too excited in the illistration?

Ecology, Types of survivorship curves

Types of survivorship curves

Explain the rotary dryers, Explain the Rotary Dryers? Rotary dryers are...

Explain the Rotary Dryers? Rotary dryers are widely used to dry relatively large throughputs of granular products and by products in a number of industries, including the food

Agro industrial-animal wastes, Animal wastes Large quantities of anima...

Animal wastes Large quantities of animal wastes are available in the country but generally they are low in nutrient content with poor feeding value to the animals. However, po

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd