Explain the indole production test, Biology

Assignment Help:

Explain the Indole Production Test?

Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.


Related Discussions:- Explain the indole production test

Determine the organs of the digestive system of earthworms, Which are the c...

Which are the characteristics and organs of the digestive system of earthworms related to the type of diet of these animals? Earthworms eat decomposing organic material and sma

Explain cosmid, Cosmid: The type of artificially constructed vector taken ...

Cosmid: The type of artificially constructed vector taken in use for cloning the 35-45 kb of DNA. These are plasmids carrying the phage l cos site (which permits packaging into l

Skin, physiology of skin

physiology of skin

What are gametes, What are gametes? Gametes are cells specialized in s...

What are gametes? Gametes are cells specialized in sexual reproduction. They have half of the maximum number of chromosomes of the species and unite with another gamete giving

What is osmotic pressure, Q What is osmotic pressure? Osmotic pressure ...

Q What is osmotic pressure? Osmotic pressure is the pressure produced in an aqueous solution by a region of lower solute concentration upon a region of higher solute concentrat

Population dispersal, Population Dispersal Population dispersal i...

Population Dispersal Population dispersal is the movement of individuals into or out of the population or the population area. It occurs in three following ways in a popu

Biological sickness of soil, what is biological sickness of soil and its ma...

what is biological sickness of soil and its management

Explain foaming properties of proteins, Explain Foaming Properties of prote...

Explain Foaming Properties of proteins To understand the foaming properties of proteins, we need to know some basic aspects of foam foods. Foam foods are usually colloidal disp

Reactants of photosynthesis, what are the reactants and the products of pho...

what are the reactants and the products of photosynthesis? under the thylakoid, stroma,and combined

How vitamins & minerals work together with nutrients in food, How Vitamins ...

How Vitamins and minerals work together with nutrients in food? Vitamins and minerals work together with nutrients in food. While antioxidant vitamins E and C and beta-carotene

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd