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Explain the Indole Production Test?
Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.
During residency: This alters from program to program depending on the number of sites covered and number of residents. At McMaster, we do call roughly 1 in 7 or 8 (averages out to
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AUTOTROPHI C NUTRITION Preparation of organic food from the inorganic materials in the living body. May be photoautrophic, e.g. Euglena virdisima. May be chemo
Give three examples of human disorders which are caused by the action of a single pair of alleles. In every case say whether the harmful allele is dominant or recessive to the non-
what is he importance of proteins for living beings
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