Explain the indole production test, Biology

Assignment Help:

Explain the Indole Production Test?

Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.


Related Discussions:- Explain the indole production test

Supernumerary or accessory chromosomes, SUPERNUMERARY OR ACCESSORY CHROMOSO...

SUPERNUMERARY OR ACCESSORY CHROMOSOMES Wilson (1905) discovered them in Matapodium insect. Very small Chromosomes present in nucleus in addition to normal number of Chr

Isospora, ciclo vital de la isospora belli

ciclo vital de la isospora belli

Protozoa, the function of contractil vacuole

the function of contractil vacuole

Brief descriptions of how to test, If sugar is heavier than aspartame, then...

If sugar is heavier than aspartame, then the can of regular soda will sink. How will you test this hypothesis? Write brief descriptions of how you could test?

Define the term - soil heterogeneity, Define the term - soil heterogeneity ...

Define the term - soil heterogeneity The ratings seem rather arbitrary and empirical but are meant to get a reasonable yield. Outputs for different doses of fertilizers are obt

Difference between ectoparasite and endoparasite, Q. What is the difference...

Q. What is the difference between ectoparasite and endoparasite? The Ectoparasites are parasites that explore the external surface of the host (like, for instance, mites that p

What is the binding between two amino acids called, What is the binding bet...

What is the binding between two amino acids called? The chemical bond among two amino acids is called a peptide bond. Protein Structure Review - Image Diversity: peptide bo

Air movements at tropical latitudes, Air movements at tropical latitudes ...

Air movements at tropical latitudes Let us now explain air movements at tropical latitudes. The surface air that rushes to fill the equatorial void from the north is deflected

Define abbe condenser of microscope, Define Abbe Condenser of Microscope? ...

Define Abbe Condenser of Microscope? It is present beneath the stage, as shown in Figure. It collects and focuses a cone of light on the slide. Its position can be adjusted ver

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd