Explain the indole production test, Biology

Assignment Help:

Explain the Indole Production Test?

Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.


Related Discussions:- Explain the indole production test

Define nutritional management of eating disorders, Define Nutritional Manag...

Define Nutritional Management of Eating Disorders? In the last unit, we focused on weight management. It is important to understand that the problems associated with too much d

ATP FORMATION, what compound is phosphorylated for ATP? what is the resulti...

what compound is phosphorylated for ATP? what is the resulting compound when ATP releases energy

Risk during crown removal-endodontics principles, Risk during crown removal...

Risk during crown removal -Tooth inadvertently extracted using a crown\bridge remover . -Endodontic therapy was performed , and the tooth was replanted , a procedure known

Inter-relationships of classification, Inter-relationships of Classificatio...

Inter-relationships of Classification The aim of classification is to put together organisms on the basis of their similarities. But the question is what type of similarities?

What is osmotic pressure, Osmotic pressure is the pressure formed in a aque...

Osmotic pressure is the pressure formed in a aqueous solution by a region of lower solute concentration upon a region of superior solute concentration forcing the passage of water

Animal diseases, Animal Diseases - Diseases are common in domestic anim...

Animal Diseases - Diseases are common in domestic animals. Common signs of illness in animals are - 1.      Cessation of rumination. 2.      Drop in milk yield. 3.

What does boiling do to an enzyme, What does boiling do to an enzyme? Enz...

What does boiling do to an enzyme? Enzymes are denatured (their shape is changed so it doesn't work) when boiled.

Protozoa, what is the life cycle of a protozoa

what is the life cycle of a protozoa

How hyperventilation affect blood ph, Freediving is an activity in which a ...

Freediving is an activity in which a person dives, sometimes to great depth, without the use of scuba gear. The diver must hold his or her breath for the duration of the dive. (The

Head examination of new born, Head - check for size, shape or fontan...

Head - check for size, shape or fontanelles, caput, or any other abnormality. Fontanel - anterior fontanel is diamond shaped having size of 2.5 - 4.5 cms. It close

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd