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Explain the Indole Production Test?
Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.
Cornea - Organogenesis of Eye and Limb The fully formed cornea contains 2-cell layered epithelium and the stroma made of extra cellular secreted materials. The bulk of the
Fever influenza
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Determine the significance of Mesoglea. The jellylike layer found between endodermal and ectodermal cell layers of diploblastic organisms. It acts as a type of cement holding t
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Explain Iron Balance and Regulation of Iron Absorption? The body has three unique mechanisms for maintaining iron balance. The first is the continuous reutilization of iron fro
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