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Explain the Indole Production Test?
Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.
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AMNIOCENTOSI S - Transabdominal aspiration of fluid from the amniotic sac of the foetus is called amniocentesis . It is prenatal diagonestic technique to determine genet
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Permanent tattoos are made by injecting pigment into the skin. Into which part of the skin is the pigment injected, the epidermis or dermis?
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If children obtain half their genes from one parent and half from the other parent, why aren't siblings identical?
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