Explain the indole production test, Biology

Assignment Help:

Explain the Indole Production Test?

Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.


Related Discussions:- Explain the indole production test

Describe class ophiuroidea in general way, Describe Class Ophiuroidea in ge...

Describe Class Ophiuroidea in general way? Brittle stars have a central disc with five long, thin, flexible arms that can be detached from the body at will in times of danger.

Evolution the eukaryotic cell or the prokaryotic cell, Which type of cell c...

Which type of cell came first in evolution the eukaryotic cell or the prokaryotic cell? Ans) This is an interesting problem of biological evolution. The most accepted hypothesis

Evaluating the degree of urinary obstruction, Evaluating the degree of urin...

Evaluating the degree of urinary obstruction: D.P. is a 63-year-old man who has been experiencing progressive difficulty with initiating the urinary stream and frequently needs to

Explain the physiological effects of ingestion of fibres, Explain the Physi...

Explain the Physiological effects of ingestion of fibres? Physiological effects of ingestion of fibres with adsorption properties are: 1. Diminished absorption of lipids. Wi

State in brief about the alexander luria, State in brief about the alexande...

State in brief about the alexander Luria Luria adopted or developed these items over the course of many years, Christensen published them in English but without standardisatio

What are some factors that can lead to protein denaturizing, Protein denatu...

Protein denaturizing can be source by temperature variation, pH change, and changes in the concentration of surrounding solutes and by other processes. Most proteins are denatured

Define ascorbic acid content of white blood cells, Define Ascorbic acid con...

Define Ascorbic acid content of white blood cells? The ascorbic acid content of white blood cells and platelets is a good index of the tissue levels of ascorbic acid. The level

What is hypercholeslerolaemia, Q. What is hypercholeslerolaemia? Choles...

Q. What is hypercholeslerolaemia? Cholesterol: It is a natural component of foods such as mutton, pork, ham, sausages, lamb, chicken, eggs (yellow), whole milk, cheese, ice-cre

Explain about the primary protein derivatives, Explain about the primary pr...

Explain about the primary protein derivatives? a) Proteins: These are the insoluble products which result from the incipient action of very dilute acids or enzymes. e.g. casein

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd