Explain the indole production test, Biology

Assignment Help:

Explain the Indole Production Test?

Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.


Related Discussions:- Explain the indole production test

What is the call frequency, During residency: This alters from program to p...

During residency: This alters from program to program depending on the number of sites covered and number of residents. At McMaster, we do call roughly 1 in 7 or 8 (averages out to

Water reuse and recycling, Water Reuse And Recycling Are Becoming   Much Mo...

Water Reuse And Recycling Are Becoming   Much More Common As Demands For Water Exceed Supply. Unplanned reuse occurs as the result of waste effluents entering receiving waters or g

Define the term- ultrafiltration, Define the term- Ultrafiltration Ultr...

Define the term- Ultrafiltration Ultrafiltration occurs when the flow of a substance across a cell membrane is increased by a hydrostatic pressure. This process was initially b

Write the definition of counselling, Q. Write the definition of counselling...

Q. Write the definition of counselling? Definition of counselling "A process of helping or enabling a person / persons to solve certain interpersonal, emotional problems and

Anatomical reason for the left bronchus to be more elevated, Q. What is the...

Q. What is the anatomical reason for the left bronchus to be more elevated than the right bronchus? Why in most cases of aspiration of foreign material by children is the object fo

Explain about the plateau effect - energy balance, Explain about the platea...

Explain about the plateau effect - Energy Balance? You must have noticed that when people start following weight reducing diets, they lose weight rapidly in the beginning, then

Vertebrate eye - adult eye, Vertebrate Eye - Adult Eye There are no m...

Vertebrate Eye - Adult Eye There are no main differences in the structure or composition of the eyes among different vertebrates. This should be clear to you from the diagram

Nutrition - autotrophic nutrition, AUTOTROPHI C NUTRITION Pr...

AUTOTROPHI C NUTRITION Preparation of organic food from the inorganic materials in the living body. May be photoautrophic, e.g. Euglena virdisima. May be chemo

Single pair of alleles, Give three examples of human disorders which are ca...

Give three examples of human disorders which are caused by the action of a single pair of alleles. In every case say whether the harmful allele is dominant or recessive to the non-

Proteins, what is he importance of proteins for living beings

what is he importance of proteins for living beings

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd