Explain the indole production test, Biology

Assignment Help:

Explain the Indole Production Test?

Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.


Related Discussions:- Explain the indole production test

Explain the energy requirements during sepsis, Explain the Energy Requireme...

Explain the Energy Requirements during Sepsis? Patients suffering from septicemia with or without MODS are generally hyper-metabolic which results in weight loss. Critically il

Explain the term- loss-of-function mutation, Where would a predicted silent...

Where would a predicted silent mutation have to be situated to actually result in a loss-of-function mutation (and potentially lead to the onset of disease)? A. Intron-exon jun

Cells, Cell theory exception

Cell theory exception

Define the dietary recommendations for epilepsy, Define the Dietary Recomme...

Define the Dietary Recommendations for epilepsy? A 3: 1 ketogenic diet is recommended. The time taken for reversing the usual ratio of 1:3 to 3: 1 is about 4 days. How will we

Define glycolysis, Define glycolysis Glycolysis or the citric acid cy...

Define glycolysis Glycolysis or the citric acid cycle in which the direction of the reactions is well defined, the  interconversion reactions of the HMP pathway can function

When the plant transpiration increases, When air humidity is high does the ...

When air humidity is high does the plant transpiration increase or lower? When air humidity is high transpiration diminishes. As transpiration is a simple diffusion process it

Karyokinesis, At the beginning of the process in an animal cell, the partit...

At the beginning of the process in an animal cell, the partition of the centriole takes place, which has been duplicated during Interphase but were in the same centrosome. Bipol

How are the male gametes of gymnosperms formed, Q. How are the male gametes...

Q. How are the male gametes of gymnosperms formed? What is the relationship between the pollen grains and the concept of alternation of generations? In the male strobiles (cone

Define risk factors for drug nutrient interaction, Define Clinical Signific...

Define Clinical Significance and Risk Factors for Drug-Nutrient interactions? We are already aware of the fact that poor nutritional status can impair drug metabolism and the d

How dissimilar are cnidarians from poriferans, Q. Concerning tissue complex...

Q. Concerning tissue complexity how dissimilar are cnidarians from poriferans? Poriferans present only some dispersed specialized cells with no tissue differentiation, Cnidaria

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd