Explain the indole production test, Biology

Assignment Help:

Explain the Indole Production Test?

Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.


Related Discussions:- Explain the indole production test

Risk of embolization, Systemic embolization occurs in 22 per cent to 50 per...

Systemic embolization occurs in 22 per cent to 50 per cent of cases of IE. Emboli often involve major arterial beds, including lungs, coronary arteries, spleen, bowel, and extremit

Function of amino acids, FUNCTIO N OF AMINO ACIDS Proteins are poly...

FUNCTIO N OF AMINO ACIDS Proteins are polymers of amino acids. Glycine form porphyrin nucleus in chlorophyll and heme (= haem) proteins like haemoglobin and cytochrome

Types of mechanism - stress condition, Types of Mechanism - Stress Conditio...

Types of Mechanism - Stress Condition Plants have evolved a variety of mechanisms to withstand stress conditions. In many cases we can precisely define the way plants enable t

Describe the neuropsychological assessment of infants, Describe the Neurops...

Describe the Neuropsychological Assessment of Infants Neuropsychological Assessment of Infants and Young Children followed by the developmental Concepts Unique to Infants and

Regernation, Give ditail account on regernation in invertebrates and verta...

Give ditail account on regernation in invertebrates and vertabrites

Handling animals in lab, Handling : The desire for survival is same whethe...

Handling : The desire for survival is same whether in animals or human beings. You may be aware of the ability of even tiny animals to scratch and bite. So we won't say any more o

Dracunculiasis (guineaworm infestation), Dracunculiasis (guineaworm infesta...

Dracunculiasis (guineaworm infestation) Dracunculiasis, a disease of man, which has been known since antiquity, is caused by the nematode parasite Dracunculus medinesis. The p

Explain nutrition diagnosis, Explain Nutrition diagnosis Nutrition dia...

Explain Nutrition diagnosis Nutrition diagnosis is the identification and labelling that describes an actual occurrence, risk of, or potential for developing a nutrition probl

Endomitosis, #questwhy and where endomitosis happens?ion..

#questwhy and where endomitosis happens?ion..

Understanding life, UNDERS T ANDIN G LIF E - Presence of proto...

UNDERS T ANDIN G LIF E - Presence of protoplasm is the important feature of life which acts the site of metabolism. Maintenance of life by protoplasm requires conti

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd