Explain the indole production test, Biology

Assignment Help:

Explain the Indole Production Test?

Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.


Related Discussions:- Explain the indole production test

Karyotype, KA R YOTYPE External morphology of Chromosomes specific...

KA R YOTYPE External morphology of Chromosomes specific for each species of living organisms. Karyotype can be studied in metaphase of mitosis. Karyotype includes th

Show some evolutionary advantages of animals, Q. What are some evolutionary...

Q. What are some evolutionary advantages of animals with complete digestive tube? The complete digestive tube permits animals to continuously feed themselves without waiting fo

How are the heterotrophic beings divided, How are the heterotrophic beings ...

How are the heterotrophic beings divided in the ecological study of food interactions? The Heterotrophs are divided into consumers and decomposers. An ecosystem can survive wit

Body temperature and room temperature , You ran both a body temperature and...

You ran both a body temperature and room temperature test for each enzyme you tested. Did the two temperatures seem to have an effect on the enzyme Exercise? Why or why not?

Hypothesis - nursing research, Hypothesis: The  spelling of hypothesis...

Hypothesis: The  spelling of hypothesis when written  as  "hypotheses"  indicate plural. The hypothesis is a statement or declaration of  the expected outcome of a research st

Effects of insulin on carbohydrate metabolism, Insulin promotes muscle gluc...

Insulin promotes muscle glucose uptake and metabolism. In presence of insulin muscle cells take up glucose and use it as a source of energy. Insulin also promotes storage of insuli

Explain nutritional support management - cancer patient, Explain about the ...

Explain about the Nutritional Support Management? Tube feeding is usually started. If tube feeding is not possible, parenteral nutrition through peripheral vein or through the

Explain the different types of cells in cell structure , Explain the differ...

Explain the different types of cells in cell structure ? Types of Cells :  Cells can be classified as being either prokaryotic or eukaryotic depending on whether or not a mem

Multiple membrane-spanning proteins, Various integral proteins have multipl...

Various integral proteins have multiple membrane-spanning   -helices. Bacteriorhodopsin, a protein found in a photosynthetic bacterium captures the energy from light and uses it to

Becoming online tutor, how can i work with u as an online biology tutor?

how can i work with u as an online biology tutor?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd