Explain the indole production test, Biology

Assignment Help:

Explain the Indole Production Test?

Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.


Related Discussions:- Explain the indole production test

Respiration, what is known as respiration in animals?

what is known as respiration in animals?

What are the classes of lipoproteins in the blood, Q. What are the classes ...

Q. What are the classes of lipoproteins in the blood? Blood lipids (cholesterol, triglycerides and phospholipids) being insoluble in blood need a ship to travel in our body; it

Tolerance range, what is the tolerance range of man and tillapia

what is the tolerance range of man and tillapia

What is g-protein, Which of the following is true for a G-protein? A. A...

Which of the following is true for a G-protein? A. After the ATP-ase of the alpha subunit of a G protein converts the ATP bound to the alpha subunit to ADP and inorganic phosph

Which are the germ layers present in cnidarians, Q. Which are the germ laye...

Q. Which are the germ layers present in cnidarians? Which tissues of the animal do they originate? These beings present endoderm and ectoderm, two germ layers. Animals only wit

Show the process of synaptic transmission, Q. Fluoxetine is an antidepressa...

Q. Fluoxetine is an antidepressant drug that presents an action mechanism related to the synaptic transmission. What is that mechanism? Fluoxetine is a substance that inhibits

Determine the term - chlorine, Determine the term - Chlorine   The appl...

Determine the term - Chlorine   The application of chlorides results in leaves of greater spread, a lighter green colour and with a smoother surface. However, the combustibilit

Tanco beans, what is the origin, uses, morphology, active constituents and ...

what is the origin, uses, morphology, active constituents and market perparations ?

Define the mechanism of evolution, A few statements about the mechanism of ...

A few statements about the mechanism of evolution are made. Mark the correct statements.   (i)  Evolution happens at the individual level as well as population level. (ii

Management of gout, Q. Management of Gout? The goals of therapy or mana...

Q. Management of Gout? The goals of therapy or management of gout is based on the following aspects: • early resolution of inflammation, • prevention of recurrent attac

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd