Explain the indole production test, Biology

Assignment Help:

Explain the Indole Production Test?

Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.


Related Discussions:- Explain the indole production test

Mitral valve replacement, Mitral Valve Replacement :  When mitral...

Mitral Valve Replacement :  When mitral valve replacement for a calcified, stenotic valve is done, usually it is a classical procedure removing leaflets, their chordae an

Explain food sources of lron, Explain Food Sources of lron? lron is fo...

Explain Food Sources of lron? lron is found in foods in one of the two forms i.e. haem or non-haem. In the human diet, the primary sources of haem iron are the haemoglobin and

What are the pupillary defects, What are the Pupillary defects There ar...

What are the Pupillary defects There are certain deviations  from normal pupillary reflexes which point towards certain specific pathology.

Functions of protein, FUNCTION S OF PROTEIN Proteins from the collo...

FUNCTION S OF PROTEIN Proteins from the colloidal complex of the protoplasm and its organelles. Most Abundant Protein in Organic World is RUBISCO or Ribulose biphosp

Name three effects that inbreeding can have on a population, Name three eff...

Name three effects that inbreeding can have on a population. Inbreeding can decrease the number of offspring produced, increase susceptibility to disease, reduce genetic varia

What are the functions of the osseous tissue, What are the functions of the...

What are the functions of the osseous tissue? The major functions of the osseous tissue are: to give structural rigidity to the body and to delineate the spatial positioning of

Determine the way to verify the genotype of that animal, Some animals, such...

Some animals, such as cows, normally make only one offspring from each mating. If a cow showed a dominant phenotype, why would a typical testcross be a complex way to verify the ge

Why the ph of urine can be quite variable, The ph of urine can be quite var...

The ph of urine can be quite variable. Why? Why is bromothymol blue used as a ph indicator instead of phenal red when titrating blood?

What is name for interruptions for myelin sheath, In most axons, myelin she...

In most axons, myelin sheath is interrupted at intervals of about 1 millimeter or more. These interruptions are known as: a) Collaterals b) Nodes of Ranvier (pron: ron-vee-ay)

Do sponges have circulatory system, Q. Do sponges have circulatory, nervous...

Q. Do sponges have circulatory, nervous and excretory systems? Sponges do not have a nervous system neither excretory system nor circulatory system.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd