Explain the indole production test, Biology

Assignment Help:

Explain the Indole Production Test?

Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.


Related Discussions:- Explain the indole production test

Can you explain likar system with electrodes, Q. Can you explain Likar Syst...

Q. Can you explain Likar System with Electrodes? The 12 lead Mason - Likar system with electrodes is used. The plastic adhesive electrodes has self contained electrode jelly in

Explain about the alitame - artificial sweeteners, Explain about the Alitam...

Explain about the Alitame - artificial sweeteners? Alitame (L-α-aspartyl-N-(2, 2, 4, 4-tetramethyl-3-thietanyl)-D-alaninamide), as illustrated in figure, is a sweetener based o

State about the importance of potassium, State about the importance of Pota...

State about the importance of Potassium  Potassium is the third major element required for plant growth, generally absorbed as K + ion. In soil 95 to 99 percent of potassium e

Prothoracic gland or ptc, Prothoracic Gland or PTC Prothoracic gland ...

Prothoracic Gland or PTC Prothoracic gland (PTC) or moulting gland and ecdysone or moulting hormone The 3 rd endocrine gland-prothoracic gland- is an irregular branching

What thiamine defiency will result in decreased activity, A thiamine defien...

A thiamine defiency will result in decreased activity of: -G-6 phosphase -transketolase -fructokinase

The kidney, diseases of the kidney and their remedies

diseases of the kidney and their remedies

Hypothetical bacterial cell, A microbiology student isolated a hypothetical...

A microbiology student isolated a hypothetical bacterial cell that seems to quadruple every generation. Knowing that binary fission is an exponential function, derive a mathematica

Will argon tend to form bonds with other elements, The atomic number of arg...

The atomic number of argon is 18. Will argon tend to form bonds with other elements? Describe your answer. Argon will not tend to form bonds with other elements. With an atomi

What is the minimal space between implants and implant, Minimal space betwe...

Minimal space between implants and implant and adjacent tooth It is important to maintain a distance of at least 1.5 to 2 mm between an implant and adjacent tooth and a distanc

Requirements of glutamine in diverticular disease, Q. Requirements of Gluta...

Q. Requirements of Glutamine in diverticular disease? Glutamine: Wile specific nutrients that may have an impact on diverticula disease have not been studied as thoroughly as t

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd