Explain the indole production test, Biology

Assignment Help:

Explain the Indole Production Test?

Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.


Related Discussions:- Explain the indole production test

Species concept, SPECIE S CONCEPT - According to Davis and Heywood...

SPECIE S CONCEPT - According to Davis and Heywood species define as assemblage of individuals with morphological features in common and separable from other such assembla

Explain the functionality of hot air oven, Explain the Functionality of Hot...

Explain the Functionality of Hot Air Oven? Hot air oven sterilizes the object by hot dry air. It kills the microorganisms by oxidizing cellular constituents. Dry Heat is less e

Free-hand sectioning of plant tissue (stem), What are the processes involve...

What are the processes involved in the preparation of plant tissue for free hand sectioning?

How do the climate affect the growth of population, Q. How do the availabil...

Q. How do the availability of water and light and the climate affect the growth of a population? The availability of light and water and the climate are abiotic factors that li

Neo darwinism or synthetic theory of evolution, NEO DARWINISM OR SYNTHETIC ...

NEO DARWINISM OR SYNTHETIC THEORY OF EVOLUTION - It is the synthesis of the ideas of Neo Darwinians as a new theory of evolution based upon recent findings of genetics and m

Engler and prantls system of classification, Q. Engler and Prantl's System ...

Q. Engler and Prantl's System of Classification? Adolf Engler, Professor of Botany, University of Berlin, Germany, proposed a phylogenetic system of classification in a book en

Illustrate glycolysis within the mitochondria, Q. Does glycolysis take plac...

Q. Does glycolysis take place within the mitochondria? Glycolysis happens in the cytosol and not in the mitochondria. Pyruvic acid molecules later enter mitochondria to partici

Updating by deletion, Updating by Deletion DELETE FROM ENROLMENT WHERE...

Updating by Deletion DELETE FROM ENROLMENT WHERE StudentId = SID ('S4'); As you can see, this differs from Example by the addition of the noise word FROM. The expression SI

Define steps in the development of exchange list, Define Steps in the Devel...

Define Steps in the Development of Exchange List? As mentioned above, when we group together similar food items so that each supplies a constant amount of a particular nutrient

Blood clotting, BLOO D CLOTTING - It is a nature's device to check ...

BLOO D CLOTTING - It is a nature's device to check the excessive loss of blood from an injury. Bleeding time is 1-3 minutes. Clotting time is 2-6 minutes. Process of

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd