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Explain the Indole Production Test?
Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.
Structure of the Conduction System of the Heart Sinoatrial (SA) Node is the pacemaker node located in the right atrium near the opening of superior venacava. It is called
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The stepped steel shaft illustrated in Figure is subjected to a torque (T) at the free end and a torque (2T) in the opposite direction at the junction of the two sizes.
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