Explain the indole production test, Biology

Assignment Help:

Explain the Indole Production Test?

Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.


Related Discussions:- Explain the indole production test

Complications of making a definite classification, Assume for this question...

Assume for this question that we are discussing a rare human disorder. Describe as detailed as possible the characteristics of this disorder if it is: autosomal dominant autosomal

Rk, how many chromosomes in human

how many chromosomes in human

How do taenias classify according to the division of sexes, How do taenias ...

How do taenias classify according to the division of sexes? Taenias are monoecious (hermaphrodite), the similar individual has female and male reproductive organs and undergoes

What do you mean by proteinuria, Q. What is proteinuria? Why is proteinuria...

Q. What is proteinuria? Why is proteinuria a sign of glomerular renal injury? Proteinuria means losing of proteins through urine under normal conditions proteins are too big to

Preliminary screening stage of battery, Preliminary screening stage of batt...

Preliminary screening stage of battery During the preliminary screening stage in the development of the battery, items in the original pool that did not attain satisfactory int

Vasa recta - vertebrate kidney, Normal 0 false false false ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

What is a stream, What is a stream? A stream is a incessant series of by...

What is a stream? A stream is a incessant series of bytes that flow into or out of your program. Input and output from devices like the mouse, , disk, keyboard, screen, modem, a

Explain the process of metastasis, Explain the process of metastasis. Defin...

Explain the process of metastasis. Define anti-angiogenic drugs and relate the role of medical imaging, if any, in anti-angiogenic drug therapy. State how you envision your particu

Non-striated or smooth muscles, NONSTRIATED (= SMOOTH) MUSCLES - Non...

NONSTRIATED (= SMOOTH) MUSCLES - Non-striated muscles are found in the posterior part of oesophagus, stomach, intestine, lungs, urinogenital tract, urinary bladder, blood ve

Which are the most important inorganic molecular substances, Q. Which are t...

Q. Which are the most important inorganic molecular substances for living beings? Ans. The most important inorganic substances for living beings are mineral salts, water,

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd