Explain the indole production test, Biology

Assignment Help:

Explain the Indole Production Test?

Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.


Related Discussions:- Explain the indole production test

Define the term diffusion, Define the terms diffusion, osmosis and filtrati...

Define the terms diffusion, osmosis and filtration. Explain why each process is important for the human body.

What percentage of the offspring will be tall, Tall (T) plants are dominant...

Tall (T) plants are dominant and short (t) plants are recessive. Two heterozygous tall plants are crossed. What percentage of the offspring will be tall?

Protist, Members of phylum animal like protist that are only free living

Members of phylum animal like protist that are only free living

Define promoting food security to control under nutrition, Define Promoting...

Define Promoting food security to control under nutrition? Public distribution of food grains through a network of ration shops, particularly to reach the population below the

Lymph capillaries, Normal 0 false false false EN-IN X...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Define arterial vessels - arterioles and arteries, Q. What are arterial ves...

Q. What are arterial vessels, arterioles and arteries? Arterial vessels are every blood vessel that carries blood from the heart to the tissues, arterioles and Arteries are art

Enzymes, what is the composition of enzymes ?

what is the composition of enzymes ?

How do the bacteria reproduce, Q. How do the bacteria reproduce? Bacter...

Q. How do the bacteria reproduce? Bacteria reproduce by binary fission termed as scissiparity. Some bacteria however present a kind of sexual reproduction transformation, conju

Flame cells, where are flame cells present?

where are flame cells present?

List the functions of insulin, Q. List the functions of insulin? a) Ins...

Q. List the functions of insulin? a) Insulin controls the level of the sugar (glucose) in the blood. b) Insulin permits cells to use sugar (glucose) for energy. Cells cannot

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd