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Explain the Indole Production Test?
Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.
what are diploblastic organisms?
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Conservation of biodiversity is very important to maintain the ecological balance of ecosystem. Various steps for conservation of biodiversity: 1. Creation of consc
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What are venous vessels, veins and venules? Venous vessels are each blood vessel that carries blood from the tissues to the heart. Veins and venules are venous vessels. Venules
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Types of Surgery There may be two options for surgical correction. Urinary Diversion In this following procedures are performed: 1) Uretero sigrnoidostomy if there is
Cinemicrography Earlier it was impossible to follow the evenvts within the cell due to: i) non-visibility of cell organelles under a light microscope, ii) maintenance of cells
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