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Explain the Gram Staining technique?
Gram staining is a differential staining procedure which divides bacteria into two groups: Gram positive and Gram negative, based on staining properties. The gram stain was developed in 1884 by the Danish Physician, Dr. Christian Gram. It is one of the most important and widely employed staining procedure in bacteriology.
It is very useful and is almost essential in identifying an unknown bacteria. The basis of gram staining is the difference in cell wall composition of two types of bacterial cells. Gram positives have thick cell wall, made up of multi-layered peptidoglycan with extensive cross-linking. On the other hand, gram negative cell wall has a very thin peptidoglycan layer surrounded by outer lipid rich outer membrane. There is no outer membrane in case of gram positives. This difference in cell wall leads to decolourization of gram negatives but not gram positives by decolourizing agent i.e., ethanol or acetone.
What is ascaris? What is the disease caused by this worm? Ascaris, or Ascaris lumbricoides, is an animal of the nematode phylum, i.e., a roundworm. Ascaris causes ascariasis, a
Liver function test should be done for all diabetic patients. This will help the doctor in the choice of drugs for the treatment of diabetes since liver abnormalities influence the
Q. Can solutions with the same concentration of diverse solutes have different osmotic pressures? The osmotic pressure of a solution does not depend on the nature of the solute
Which of the following E. coli can utilize lactose? Explain your answer. (-) superscript means mutation in the gene. Explain why the strain is able or unable to utilize la
Hemiplegic migraine and ophthalmologic migraine These are relatively rare and include loss of movement of the limbs and eyes, respectively.
Complications of Exchange Transfusion i) Infection of umbilical vessels and systemic injection related to blood transfusion like malaria, CMV, AIDS and hepatitis B. ii) O
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Define Foods Effect on Drug Transport? Many drugs are transported in blood bound lo plasma proteins. Severe malnutrition or diseases affecting the synthesis of plasma proteins
1. Briefly explain the dynamics in enzyme reaction mechanism with potential energy surface diagram. 2. How to find the rate constant of any enzymetic reaction? 3. Derivation
SHARPS : Cuts due to careless handling of sharps such as sectioning razors, microtome blades etc. are probably the most common cause of injury in the biology lab. The only real re
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