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Explain the Gram Staining technique?
Gram staining is a differential staining procedure which divides bacteria into two groups: Gram positive and Gram negative, based on staining properties. The gram stain was developed in 1884 by the Danish Physician, Dr. Christian Gram. It is one of the most important and widely employed staining procedure in bacteriology.
It is very useful and is almost essential in identifying an unknown bacteria. The basis of gram staining is the difference in cell wall composition of two types of bacterial cells. Gram positives have thick cell wall, made up of multi-layered peptidoglycan with extensive cross-linking. On the other hand, gram negative cell wall has a very thin peptidoglycan layer surrounded by outer lipid rich outer membrane. There is no outer membrane in case of gram positives. This difference in cell wall leads to decolourization of gram negatives but not gram positives by decolourizing agent i.e., ethanol or acetone.
Occurrence of Jaundice Excessive production of bilirubin - this occurs in some diseases due to destruction of large number of red blood cells in the liver. Obstruction to
South Dakota and Oregon are at similar latitudes, and yet, on average, Oregon has warmer winters and cooler summers than South Dakota does. What property of water helps account for
State the Layers of retina The retina is coil~posed of ten layers: a) Pigmented epithelium (outermost layer), b) Neusoepithelium, rods and cones (photoreceptors), c) Exte
Respiration in animals
The standard free energy change and standard activation energy for four biochemical reactions are listed in the table below: A few interpretations are given below. Between thes
A DNA library is a collection of cloned DNA fragments in a cloning vector which can be fined for a DNA of interest. If the target is to isolate particular gene sequences.
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Q. What is function of Gamaaminobutyric acid? GABA is the principal inhibitory transmitter in the brain. The concentration of GABA is up to 1000 times greater than that of oth
Q. How does digestion in beings of the phylum Annelida work and which type of digestive system do they have? Digestion in beings of the phylum Annelida is extracellular. These
ROLE OF ENZYMES IN METABOLISM Enzymes require a non-protein part for their optimal activity, which may be a coenzyme or a metal ion. This has also been stated earlier that
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