Explain the gram staining technique, Biology

Assignment Help:

Explain the Gram Staining technique?

Gram staining is a differential staining procedure which divides bacteria into two groups: Gram positive and Gram negative, based on staining properties. The gram stain was developed in 1884 by the Danish Physician, Dr. Christian Gram. It is one of the most important and widely employed staining procedure in bacteriology.

It is very useful and is almost essential in identifying an unknown bacteria. The basis of gram staining is the difference in cell wall composition of two types of bacterial cells. Gram positives have thick cell wall, made up of multi-layered peptidoglycan with extensive cross-linking. On the other hand, gram negative cell wall has a very thin peptidoglycan layer surrounded by outer lipid rich outer membrane. There is no outer membrane in case of gram positives. This difference in cell wall leads to decolourization of gram negatives but not gram positives by decolourizing agent i.e., ethanol or acetone.


Related Discussions:- Explain the gram staining technique

Botany homework troubles., The xylem and phloem of the plant could be descr...

The xylem and phloem of the plant could be described as the "circulatory system" of the plant. However there are key differences between vascular tissue in plants and our own circu

Define proteins required for underweight - nutritional care, Define Protein...

Define Proteins required for underweight - Nutritional Care? Proteins are required for tissue building, as well as, to take care of the daily wear and tear. Under weight indivi

Experiment to test your hypothesis, Deinococcus radiodurans is a bacterium ...

Deinococcus radiodurans is a bacterium that was isolated from cooling ponds in and around nuclear power plants. It is highly resistant to ionizing radiation. Propose an hypothesis

Define elongation phase - mechanism of protein synthesis, Define Elongation...

Define Elongation phase - mechanism of protein synthesis? Elongation: RNA polymerase moves along the DNA and sequential synthesizes the RNA chain. There is the repeated cycle o

What is the tertiary structure of a protein, The tertiary protein structure...

The tertiary protein structure is a spatial conformation additional to the secondary structure in which the alpha helix or the beta-sheet folds up itself. The forces that stay the

Illustrate maintenance of high levels of dissolved solutes, Which of the fo...

Which of the following processes assist in the maintenance of high levels of dissolved solutes in the interstitial spaces of the kidney medulla? A. Net flux of sodium ions from

What is total cavo pulmonary connections, What is Total Cavo Pulmonary Conn...

What is Total Cavo Pulmonary Connections (TCPC)-Lateral Tunnel Operation? Cardiopulinonary bypass is instituted by cannulation of ascending aorta, SVC innominate vein junction

Define a technique of implant by using a tissue punch, Using a Tissue Punch...

Using a Tissue Punch The implant heads are located and using a suitable sized tissue punch(depending on the implant diameter), the tissue punch is used and takes a circular pie

Cell get oxaloacetic acid for krebs citric acid cycle, From where does the ...

From where does the cell get oxaloacetic acid for krebs citric acid cycle? Oxaloacetic acid is also formed when pyruvate is acted upon by pyruvate carboxylase in response to an

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd