Explain the gram staining technique, Biology

Assignment Help:

Explain the Gram Staining technique?

Gram staining is a differential staining procedure which divides bacteria into two groups: Gram positive and Gram negative, based on staining properties. The gram stain was developed in 1884 by the Danish Physician, Dr. Christian Gram. It is one of the most important and widely employed staining procedure in bacteriology.

It is very useful and is almost essential in identifying an unknown bacteria. The basis of gram staining is the difference in cell wall composition of two types of bacterial cells. Gram positives have thick cell wall, made up of multi-layered peptidoglycan with extensive cross-linking. On the other hand, gram negative cell wall has a very thin peptidoglycan layer surrounded by outer lipid rich outer membrane. There is no outer membrane in case of gram positives. This difference in cell wall leads to decolourization of gram negatives but not gram positives by decolourizing agent i.e., ethanol or acetone.


Related Discussions:- Explain the gram staining technique

Difference between eye and camera, Difference between Eye and Camera - ...

Difference between Eye and Camera -   Camera   Ey e 1. Box.   2. Black inner paint   3. Shutter   4. Diaph

From which structures is the nuclear membrane composed, Eukaryotic cells ha...

Eukaryotic cells have nucleus delimited by two juxtaposed membranes that start with the membrane of the endoplasmic reticulum. The nuclear membrane, or karyotheca, represents pores

Burrowing - mechanics of locomotion, Burrowing - Mechanics of Locomotion ...

Burrowing - Mechanics of Locomotion Some polychaetes are burrowing. Instance is glycerides and capitellidae. Their parapodia are smaller. Burrowing is done by protrusion of pr

Define nutritional requirement in cold and polar environment, Define Nutrit...

Define Nutritional Requirements in Cold and Polar Environment? Energy requirements are the major consideration for providing nutritional support in a cold environment. Energy e

Define dietary modifications in the diet of the elderly, Define Dietary mod...

Define Dietary modifications in the diet of the elderly? Loss of teeth due to increased decay of teeth and gums is common in aged person. Fitting dentures may also make chewin

Glutamine synthetase, Glutamine synthetase catalyzes the incorporation of a...

Glutamine synthetase catalyzes the incorporation of ammonia into glutamine and deriving energy from the hydrolysis of ATP. This enzyme is named a synthetase and rather than a synth

Mcnaughton case and law, MCNAUGHTON'S CASE AND LAW: In 1843, McNaughto...

MCNAUGHTON'S CASE AND LAW: In 1843, McNaughton shot and  killed Sir Robert Peel's secretary Edward -Drymmond.  It was  shown  that McNaughton suffered from a number of  delusi

What are the physiological systems, What are the physiological systems know...

What are the physiological systems known as integrative systems? Why is this designation justified? The integrative systems are the nervous system and the endocrine system. The

Explain syndrome x as a effect of obesity, Explain Syndrome X as a Effect o...

Explain Syndrome X as a Effect of Obesity? People with intra-abdominal obesity with high waist- to- hip ratio are more prone to develop the metabolic syndrome X. This is charac

Usefulness of bacteria in a natural environment, Give one example in each c...

Give one example in each case of the usefulness of bacteria in (a) a natural environment, (b) an industrial process.   (a) In a natural environment bacteria brin

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd