Explain the gram staining technique, Biology

Assignment Help:

Explain the Gram Staining technique?

Gram staining is a differential staining procedure which divides bacteria into two groups: Gram positive and Gram negative, based on staining properties. The gram stain was developed in 1884 by the Danish Physician, Dr. Christian Gram. It is one of the most important and widely employed staining procedure in bacteriology.

It is very useful and is almost essential in identifying an unknown bacteria. The basis of gram staining is the difference in cell wall composition of two types of bacterial cells. Gram positives have thick cell wall, made up of multi-layered peptidoglycan with extensive cross-linking. On the other hand, gram negative cell wall has a very thin peptidoglycan layer surrounded by outer lipid rich outer membrane. There is no outer membrane in case of gram positives. This difference in cell wall leads to decolourization of gram negatives but not gram positives by decolourizing agent i.e., ethanol or acetone.


Related Discussions:- Explain the gram staining technique

Evolution, describe the two scientific models for evolution (gradualism, pu...

describe the two scientific models for evolution (gradualism, punctuated equilibrium)

Oxygen transport in blood, Oxygen Transport in Blood All four respirat...

Oxygen Transport in Blood All four respiratory pigments are adapted to load and unload oxygen effectively in I the habitats where they have evolved, whether animals live on la

Skin, SKIN - Largest organ of body. It is covered by hair...

SKIN - Largest organ of body. It is covered by hairs or pelage i.e. pillifera. Study of skin is dermatology. Outermost covering of body. Skin is elastic and

Cleavage - metazoa, Cleavage - Metazoa The unicellular zygote begins c...

Cleavage - Metazoa The unicellular zygote begins cell division (cleavage). First, the single cell divides forming two cells, these redivide further to form four, then eight ce

Explain tick-borne encephalitis, Tick-Borne Encephalitis (TBE ) TBE occu...

Tick-Borne Encephalitis (TBE ) TBE occurs in Scandinavia, Western and Central Europe and countries of the former USSR, mainly in rural forested areas. Risk is greatest from Apri

Determine the effect of drug on the blood pressure of rats, A scientist wan...

A scientist wanted to study the effect of a drug on the blood pressure of rats. She set up an experiment in which the experimental group consisted of rats that were injected with a

Define role of public nutritionist in health care delivery, Define the Role...

Define the Role of Public Nutritionist in Health Care Delivery? It is clearly evident from the foregoing discussion that nutrition is an important, though not the only, determi

Explain first stage of the embryonic development, Q. What is the cell divis...

Q. What is the cell division during the first stage of the embryonic development called? How is this stage characterized? The cell division in the first stage of the embryonic

Magnesium - mineral elements, Magnesium -  It is available in most of t...

Magnesium -  It is available in most of the plants, especially vegetables. By its deficiency diarrhoea is caused. Its important functions are - (a)      Along with c

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd