Explain the gram staining technique, Biology

Assignment Help:

Explain the Gram Staining technique?

Gram staining is a differential staining procedure which divides bacteria into two groups: Gram positive and Gram negative, based on staining properties. The gram stain was developed in 1884 by the Danish Physician, Dr. Christian Gram. It is one of the most important and widely employed staining procedure in bacteriology.

It is very useful and is almost essential in identifying an unknown bacteria. The basis of gram staining is the difference in cell wall composition of two types of bacterial cells. Gram positives have thick cell wall, made up of multi-layered peptidoglycan with extensive cross-linking. On the other hand, gram negative cell wall has a very thin peptidoglycan layer surrounded by outer lipid rich outer membrane. There is no outer membrane in case of gram positives. This difference in cell wall leads to decolourization of gram negatives but not gram positives by decolourizing agent i.e., ethanol or acetone.


Related Discussions:- Explain the gram staining technique

State the disorders of sleep, State the Disorders of sleep The need for...

State the Disorders of sleep The need for sleep varies considerably from one person to another, as well as in the same person at different stages of life. We have all been told

State the purpose of a neuropsychological screening, State the purpose of a...

State the purpose of a neuropsychological screening The purpose of a neuropsychological screening examination is to determine if there is reasonable evidence, beyond initial cl

Determine the important rule of insulin syringes, Insulin Syringes Syri...

Insulin Syringes Syringes for insulin are designed for standard U-100 insulin. 1 ml of insulin has 100 standard units of insulin. Syringe has markings in insulin units it helps

Various modalities available for sterility assurance, Q. Enlist the various...

Q. Enlist the various modalities available for sterility assurance? Various modalities available for sterility assurance are a) Prevention of overloading b) Chemical indi

Explain the role of pancreas in digestion, Role of Pancreas in Digestion ...

Role of Pancreas in Digestion Pancreatic  juices  secreted from  the  pancreas  aid  in  digestion  of  the  food. About 600-800  ml  of  fluid  i.e.  the  pancreatic juice  is

What is class asteroidea, What is Class Asteroidea ? Sea stars are prob...

What is Class Asteroidea ? Sea stars are probably one of the most recognizable of all Echinoderms. Often beautifully colored and symmetrical, they are predators that feed vorac

Explain a prosthetic considerations, Prosthetic Considerations The comp...

Prosthetic Considerations The complete aesthetic diagnosis of the patient should be done prior to implant placement in the aesthetic zone. The smile line of the patient is crit

Explain preparation and properties of modified starches, Preparation and pr...

Preparation and properties of modified starches Improved performance of starches may  be realized by physical and chemical modifications. The major physically modified starch i

Explain the integrity of cells, When lipid is added to a solution of a dete...

When lipid is added to a solution of a detergent in water, the detergent breaks up large globules of the lipid into much smaller globules. What effect do you think a detergent woul

Explain the eukaryotic gene expression , Explain the Eukaryotic Gene Expres...

Explain the Eukaryotic Gene Expression ? Eukaryotic cells regulate the transcription of individual genes, large parts of chromosomes, or even entire chromosomes. Gene expressi

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd