Explain the gram staining technique, Biology

Assignment Help:

Explain the Gram Staining technique?

Gram staining is a differential staining procedure which divides bacteria into two groups: Gram positive and Gram negative, based on staining properties. The gram stain was developed in 1884 by the Danish Physician, Dr. Christian Gram. It is one of the most important and widely employed staining procedure in bacteriology.

It is very useful and is almost essential in identifying an unknown bacteria. The basis of gram staining is the difference in cell wall composition of two types of bacterial cells. Gram positives have thick cell wall, made up of multi-layered peptidoglycan with extensive cross-linking. On the other hand, gram negative cell wall has a very thin peptidoglycan layer surrounded by outer lipid rich outer membrane. There is no outer membrane in case of gram positives. This difference in cell wall leads to decolourization of gram negatives but not gram positives by decolourizing agent i.e., ethanol or acetone.


Related Discussions:- Explain the gram staining technique

Can a chemical reaction the free energy change to mol, In a chemical reacti...

In a chemical reaction the free energy change (/\Go') is -40 jk/mol and the entropy factor T/\S is 15 kj/mol. the enthalpy change /\H for the reaction is a. 25 kj/mol b.-25 k

Shivering - heat production, Shivering - Heat Production The mechanis...

Shivering - Heat Production The mechanism of thermogenesis with which we are more familiar is shivering and all adult birds and mammals seem to use these mechanism. Shivering

Wildlife - ecology, Wildlife - Ecology The term wildlife probably orig...

Wildlife - Ecology The term wildlife probably originated in 1913 in a book, Our Vanishing Wildlife by William Hornaday, Director of the New York Zoological Park. The main focu

What is parasitism, What is parasitism? Parasitism is the ecological in...

What is parasitism? Parasitism is the ecological interaction in which a being lives at the expense of another. The parasite often does not cause immediate death of the host sin

Why energy transfer between trophic level is not 100 percent, Give three re...

Give three reasons why energy transfer between trophic levels is not 100 percent. Some of the organisms in a trophic level escape being eaten; some energy is kept in molecules

An insect collecting net, An insect collecting net A useful insect net ...

An insect collecting net A useful insect net can be made from a round stick such as a broom or mop handle, some heavy wire and mosquito netting or cheese cloth. Bend a heavy pi

Define principle for iodine number estimation - lipids, Define Principle fo...

Define Principle for Iodine Number Estimation - Lipids? Iodine number is defined as the number of grams of iodine absorbed by 100 grams of fat, it gives an idea of the relative

Handling microorganisms, Handling : You can reduce direct contact with mic...

Handling : You can reduce direct contact with microorganisms by careful handling and by wearing protective clothing such as rubber gloves, mask, lab coat, etc. Even if you entirel

How many are the known amino acids, How many are the known amino acids that...

How many are the known amino acids that form proteins in living beings? There are twenty dissimilar known amino acids that form proteins related to the genetic code of the livi

Acellular and cellular organisms, Acellular and Cellular Organisms you...

Acellular and Cellular Organisms you should be able to characterise living and non- living matter. However, the boundary-line between the two is not very precise. As you have

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd