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Explain the Gram Staining technique?
Gram staining is a differential staining procedure which divides bacteria into two groups: Gram positive and Gram negative, based on staining properties. The gram stain was developed in 1884 by the Danish Physician, Dr. Christian Gram. It is one of the most important and widely employed staining procedure in bacteriology.
It is very useful and is almost essential in identifying an unknown bacteria. The basis of gram staining is the difference in cell wall composition of two types of bacterial cells. Gram positives have thick cell wall, made up of multi-layered peptidoglycan with extensive cross-linking. On the other hand, gram negative cell wall has a very thin peptidoglycan layer surrounded by outer lipid rich outer membrane. There is no outer membrane in case of gram positives. This difference in cell wall leads to decolourization of gram negatives but not gram positives by decolourizing agent i.e., ethanol or acetone.
Q. How does one get infected by Enterotoxigenic escherichia coli gastroenteritis(ETEC)? Infection occurs when a person eats food or drinks water or ice contaminated with ETEC
Diagnosis of diabetes mellitus which included history taking, symptoms and signs and various tests which can be done to diagnose a case of diabetes. Diabetes Mellitus (DM) or diabe
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Explain Coarctation of Aorta ? Coarctation of aorta may be isolated or it may have other co-existing cardiac and vascular lesions. In critically ill neonates with coarctation,
Define in brief about the biomolecules Some of the biomolecules, also known as compounds of life, form part of the structure of cell, and some of these are sources of energy fo
Sterile Technique Every effort should be made to conduct implant surgery under sterile operating conditions. Chlorhexidine 0.2% is used as a pre-operative mouthwash and skin pr
Define role of Zinc in controlling Gene Expression? Zinc atoms have specific structural roles in enzyme molecules, as well as, in many other proteins and in bio membranes. Thes
Define Pasteurization and Sterilization - thermal processing? Pasteurization: Pasteurization is a mild heat treatment to kill part of the microorganisms present in food
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P n e u mo n i a It is inflammation of pulmonary parenchyma, usually accompanied by inflammation of bronchioles, and is characterized by cough, increased respiratory rat
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