Explain the gram staining technique, Biology

Assignment Help:

Explain the Gram Staining technique?

Gram staining is a differential staining procedure which divides bacteria into two groups: Gram positive and Gram negative, based on staining properties. The gram stain was developed in 1884 by the Danish Physician, Dr. Christian Gram. It is one of the most important and widely employed staining procedure in bacteriology.

It is very useful and is almost essential in identifying an unknown bacteria. The basis of gram staining is the difference in cell wall composition of two types of bacterial cells. Gram positives have thick cell wall, made up of multi-layered peptidoglycan with extensive cross-linking. On the other hand, gram negative cell wall has a very thin peptidoglycan layer surrounded by outer lipid rich outer membrane. There is no outer membrane in case of gram positives. This difference in cell wall leads to decolourization of gram negatives but not gram positives by decolourizing agent i.e., ethanol or acetone.


Related Discussions:- Explain the gram staining technique

Genetic, what are the limitation of mendelian experiment

what are the limitation of mendelian experiment

What are trends of the gametophyte in the evolution of plant, What are tren...

What are trends of the gametophyte in the evolution of plants? The tendency of the gametophyte evolution in plants has been towards the formation of gametes that are self-dete

Does an increase of h+ cause the hemaglobin, Does an increase of H+ cause t...

Does an increase of H+ cause the hemaglobin oxygen binding curve to shift to the left or right? Explain.

Lipids, Lipids Lipids are oily or greasy organic compound forming about...

Lipids Lipids are oily or greasy organic compound forming about 3% of protoplasm. These are also compound of carbon hydrogen and oxygen but unlike carbohydrates. The hydrogen a

Nucellus - seed, Nucellus - Seed In a large majority of flowering plan...

Nucellus - Seed In a large majority of flowering plants the nucellus is gradually utilized by the endosperm or embryo. In leguminous seeds, for example, the nucellus degenerat

Wbc.., Ask question #Minimuwwm 100 words accepted#

Ask question #Minimuwwm 100 words accepted#

Sexual selection, In this section you will be introduced to a variant of Da...

In this section you will be introduced to a variant of Darwinian concept of natural selection which he termed sexual selection. You may have observed very distinct and striking dif

Explain the polycarbonate nucleopore filtre, Explain the Polycarbonate Nucl...

Explain the Polycarbonate Nucleopore Filtre? A given sample is filtered through a membrane filter, usually polycarbonate nucleopore filter with a pore size that will retain bac

How are mammals characterized, Q. Mammal identity card. How are mammals cha...

Q. Mammal identity card. How are mammals characterized according to examples of representing beings, skin, respiration, nitrogen waste, basic morphology, circulation, thermal contr

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd