Explain the gram staining technique, Biology

Assignment Help:

Explain the Gram Staining technique?

Gram staining is a differential staining procedure which divides bacteria into two groups: Gram positive and Gram negative, based on staining properties. The gram stain was developed in 1884 by the Danish Physician, Dr. Christian Gram. It is one of the most important and widely employed staining procedure in bacteriology.

It is very useful and is almost essential in identifying an unknown bacteria. The basis of gram staining is the difference in cell wall composition of two types of bacterial cells. Gram positives have thick cell wall, made up of multi-layered peptidoglycan with extensive cross-linking. On the other hand, gram negative cell wall has a very thin peptidoglycan layer surrounded by outer lipid rich outer membrane. There is no outer membrane in case of gram positives. This difference in cell wall leads to decolourization of gram negatives but not gram positives by decolourizing agent i.e., ethanol or acetone.


Related Discussions:- Explain the gram staining technique

Can you explain pneumothorax, Q. Can you explain Pneumothorax? Air in t...

Q. Can you explain Pneumothorax? Air in the pleural cavity manifests in a number of ways on the CXR, depending on the volume of air and position of the patient. The typical fin

Which are the extra abundant ribosomes in secretory cells, Q Which are the ...

Q Which are the extra abundant ribosomes in secretory cells - the free cytoplasmic ribosomes or those associated with the rough endoplasmic reticulum? Free cytoplasmic ribosome

Explain the estimated average intake (ear), Explain The Estimated Average I...

Explain The Estimated Average Intake (EAR)? Considering that the nutrient requirements follow a normal distribution, the EAR is defined as 'the median usual intake that meets t

State the term - vibrio parahaemolyticus gastroenteritis, Vibrio parahaemol...

Vibrio parahaemolyticus gastroenteritis While most other known food poisoning syndromes may be contracted from a variety of foods,  V. parahaemolyticus gastroenteritis is contr

Illustrate erythropoietin, Define Erythropoietin A. acts by stimulating...

Define Erythropoietin A. acts by stimulating the production of red blood cells by the peritubular interstitial cells of the kidney cortex. B. is secreted by cells in the bon

What is the destination of the branchial clefts in humans, Q. What is the d...

Q. What is the destination of the branchial clefts in humans? In humans the branchial clefts located in the anterior region of the pharynx also known as pharyngeal clefts are p

Explain isomer, Isomer : Existence of different  compounds having  same ...

Isomer : Existence of different  compounds having  same  molecular form but different structural forms.

Excretory system, types of excretory system of lower animals

types of excretory system of lower animals

Explain solid salt and saturated aqueous solution, Explain solid salt and s...

Explain solid salt and saturated aqueous solution? In this example, the equilibrium system consists of crystalline PbCl 2 and an aqueous phase containing the species H 2 O, P

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd