Explain the gram staining technique, Biology

Assignment Help:

Explain the Gram Staining technique?

Gram staining is a differential staining procedure which divides bacteria into two groups: Gram positive and Gram negative, based on staining properties. The gram stain was developed in 1884 by the Danish Physician, Dr. Christian Gram. It is one of the most important and widely employed staining procedure in bacteriology.

It is very useful and is almost essential in identifying an unknown bacteria. The basis of gram staining is the difference in cell wall composition of two types of bacterial cells. Gram positives have thick cell wall, made up of multi-layered peptidoglycan with extensive cross-linking. On the other hand, gram negative cell wall has a very thin peptidoglycan layer surrounded by outer lipid rich outer membrane. There is no outer membrane in case of gram positives. This difference in cell wall leads to decolourization of gram negatives but not gram positives by decolourizing agent i.e., ethanol or acetone.


Related Discussions:- Explain the gram staining technique

When the blood level of estrogen is high, Birth control pills maintain a hi...

Birth control pills maintain a high blood level of estrogen and progesterone. What is happening in the ovary when the blood level of estrogen is high? How is the uterus responding?

How do copper and hormone releasing iuds, a) How do copper and hormone rele...

a) How do copper and hormone releasing IUDs act as contraceptives? Describe. b) If you squeeze a seed of orange you may be observe many embryos of different sizes.  How is it

Define nutritional needs during recovery, Define nutritional needs During R...

Define nutritional needs During Recovery? Here, let us discuss what should be the nutritional goals based on the physiological aspects involved. Goals: The main emphasis mus

Approach grafting, Approach grafting In this method both scion and s...

Approach grafting In this method both scion and stock remain rooted. A small slice is cut off from the stem of scion and stock. Scion is bent towards the stock. The two c

#title.respiratio., i want notes for respiration in humans full qand to the...

i want notes for respiration in humans full qand to the point si i can learn them

Nuclease, Nuclease  is an enzyme which degrades the nucleic acids. A nuclea...

Nuclease  is an enzyme which degrades the nucleic acids. A nuclease can be DNA-specific (a DNase), RNA-specific (RNase) or non-specific. It might act only on single stranded nuclei

Values of dpd for plant cells under hypertonic media, Q. What are the value...

Q. What are the values of DPD for plant cells under hypertonic, hypotonic and isotonic media? In plant cells under hypertonic medium there is loss of water for the external, SF

What is portal and rental circulation, What is Portal and Rental Circulatio...

What is Portal and Rental Circulation ? The body has other circulation systems that do not return blood directly to the heart. For instance, the blood that drains from the abdo

What are the venous of the valves system, Q. What are the venous of the val...

Q. What are the venous of the valves system? What is their function? The valves of the venous system are structures inside the veins that permit blood to flow only in the norma

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd