Explain the gram staining technique, Biology

Assignment Help:

Explain the Gram Staining technique?

Gram staining is a differential staining procedure which divides bacteria into two groups: Gram positive and Gram negative, based on staining properties. The gram stain was developed in 1884 by the Danish Physician, Dr. Christian Gram. It is one of the most important and widely employed staining procedure in bacteriology.

It is very useful and is almost essential in identifying an unknown bacteria. The basis of gram staining is the difference in cell wall composition of two types of bacterial cells. Gram positives have thick cell wall, made up of multi-layered peptidoglycan with extensive cross-linking. On the other hand, gram negative cell wall has a very thin peptidoglycan layer surrounded by outer lipid rich outer membrane. There is no outer membrane in case of gram positives. This difference in cell wall leads to decolourization of gram negatives but not gram positives by decolourizing agent i.e., ethanol or acetone.


Related Discussions:- Explain the gram staining technique

Define food business and food technology, Define Food Business and Food Tec...

Define Food Business and Food Technology? The food business and food technology are practically inseparable. The food business may be characterized as: vulnerable to spoilage,

Preservation of species diversity, Q. Preservation of species diversity? ...

Q. Preservation of species diversity? Genetic diversity is, thus, important for the preservation of species diversity, and hence biological diversity. A knowledge of the variab

Nervous system control of blood pressure, Q. Nervous System control of bloo...

Q. Nervous System control of blood pressure? Most nervous controls are achieved via involuntary reflex arcs involving pressoreceptors, the vasomotor centers of the medulla, and

Genetic material, What is the central principle of molecular biology?

What is the central principle of molecular biology?

Explain the term protoderm, Explain the term Protoderm? The protoderm ...

Explain the term Protoderm? The protoderm is one of the so-called "primary tissues" because it is formed first during germination and subsequent plant growth. The protoderm g

Explain epididymitis, Epididymitis  Acute epididymitis in men less than...

Epididymitis  Acute epididymitis in men less than 35 years old is usually caused by C. trachomatis or, less frequently, N. gonorrhoeae. Older men or those who have had urinary

Sinece, modes of nutrition in animals?

modes of nutrition in animals?

What happen when the standard free energy of a reaction, When the standard ...

When the standard free energy of a reaction is -5.7 kj/mol then at equilibrium a- there will be 10 times more products than reactants b-there will be 10000 times more product

What do you mean by gametogenesis, Q. What is the kind of cell division tha...

Q. What is the kind of cell division that allows sexual reproduction? What is gametogenesis? Meiosis is the kind of cell division that permits sexual reproduction since it redu

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd