Explain the gram staining technique, Biology

Assignment Help:

Explain the Gram Staining technique?

Gram staining is a differential staining procedure which divides bacteria into two groups: Gram positive and Gram negative, based on staining properties. The gram stain was developed in 1884 by the Danish Physician, Dr. Christian Gram. It is one of the most important and widely employed staining procedure in bacteriology.

It is very useful and is almost essential in identifying an unknown bacteria. The basis of gram staining is the difference in cell wall composition of two types of bacterial cells. Gram positives have thick cell wall, made up of multi-layered peptidoglycan with extensive cross-linking. On the other hand, gram negative cell wall has a very thin peptidoglycan layer surrounded by outer lipid rich outer membrane. There is no outer membrane in case of gram positives. This difference in cell wall leads to decolourization of gram negatives but not gram positives by decolourizing agent i.e., ethanol or acetone.


Related Discussions:- Explain the gram staining technique

Describe in detail about the chlorophyll molecule, Describe in detail about...

Describe in detail about the chlorophyll molecule Each chlorophyll molecule contains magnesium ion, there could be no green plants without magnesium. As magnesium is present in

Take advantage of retro transposes in human gene therapy, In what way may w...

In what way may we be able to take advantage of retro transposes in human gene therapy? How would this differ from our current use of retroviruses?

Gastroesophageal reflux, Q. Show Gastroesophageal reflux? Obesity is th...

Q. Show Gastroesophageal reflux? Obesity is thought to be another potential predisposing factor to gastroesophageal reflux or GERD. Maintenance of ideal weight for age may help

Endocrine regulation of the cycle - reproduction, Endocrine Regulation of t...

Endocrine Regulation of the Cycle - Reproduction You have learnt above that the reproductive cycles are governed by the interplay of pituitary and gonadal hormones. According

Carbohydrates requirements for ulcerative colitis, Q. Carbohydrates require...

Q. Carbohydrates requirements for ulcerative colitis? Carbohydrates: They form the easily absorbable source of energy. Bulk-producing vegetables are restricted so as to allow b

Define the management of obesity, Define the Management of Obesity? Man...

Define the Management of Obesity? Management of obesity should be taken up with a clear understanding of the harsh realities of the problem and its outcome. It may be a frustra

Plant physiology, application of apical dominace in horticulture and agricu...

application of apical dominace in horticulture and agriculture short notes

Class turbellaria, plz answer me how I arrange my topic with its order ?

plz answer me how I arrange my topic with its order ?

Define influence of polyphenols on carbohydrates, Define influence of polyp...

Define influence of polyphenols on Carbohydrates? Binding of proteins may indirectly affect carbohydrate absorption. Inhibition of amylolytic enzymes and subsequent reduction o

Define radiological anatomy, Q. Define Radiological anatomy? The border...

Q. Define Radiological anatomy? The borders of the mediastinum on the CXR are defined by the contrast produced by adjacent air filled lung. Postero-Anterior View It in

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd