Explain the gram staining technique, Biology

Assignment Help:

Explain the Gram Staining technique?

Gram staining is a differential staining procedure which divides bacteria into two groups: Gram positive and Gram negative, based on staining properties. The gram stain was developed in 1884 by the Danish Physician, Dr. Christian Gram. It is one of the most important and widely employed staining procedure in bacteriology.

It is very useful and is almost essential in identifying an unknown bacteria. The basis of gram staining is the difference in cell wall composition of two types of bacterial cells. Gram positives have thick cell wall, made up of multi-layered peptidoglycan with extensive cross-linking. On the other hand, gram negative cell wall has a very thin peptidoglycan layer surrounded by outer lipid rich outer membrane. There is no outer membrane in case of gram positives. This difference in cell wall leads to decolourization of gram negatives but not gram positives by decolourizing agent i.e., ethanol or acetone.


Related Discussions:- Explain the gram staining technique

Rabbit femur, describe the features of the rabbit femur bone

describe the features of the rabbit femur bone

Concerning the number of hosts how are parasites classified, Q. Concerning ...

Q. Concerning the number of hosts how are parasites classified? The Parasites that require only one host are called as monoxenous parasites. The Parasites that need more than o

What is the primary energy source for life on earth, What is the primary en...

What is the primary energy source for life on earth? The primary energy resource for life on earth is the sun. The sun plays the vital role of keeping the planet warmed and it

Where in leaves the photosynthetic tissue often located, Where in the leave...

Where in the leaves is the photosynthetic tissue often located? The major photosynthetic tissue is the photosynthetic parenchyma (also called as chlorenchyma, do not confuse wi

Differentiate between the five kingdoms of organisms, Question 1: (a) G...

Question 1: (a) Give, in order, the major categories of taxonomic classification. (b) Differentiate between the five kingdoms of organisms. (c) Differentiate between net

Grazing food chain - food chain, Grazing Food Chain - Food Chain Grazi...

Grazing Food Chain - Food Chain Grazing food chains are quite familiar to most of us. Cow or deer grazing in a field represent a grazing food chain. Similarly, eating of phyto

Change in structure of a protein, Q. Is it expected that a change in the pr...

Q. Is it expected that a change in the primary, in the secondary or in the tertiary structure of a protein will produce furthermore functional consequences? Any change of the p

How does one get infected by etec, Q. How does one get infected by Enteroto...

Q. How does one get infected by Enterotoxigenic escherichia coli gastroenteritis(ETEC)? Infection occurs when a person eats food or drinks water or ice contaminated with ETEC

Necessity of transport in phloem, Necessity of Transport in Phloem Lea...

Necessity of Transport in Phloem Leaves produce photoassimilates and support various tissues including roots. The excess photoassimilates and metabolites stored in specialised

Definition of counselling, Q. Definition of Counselling? Counselling ha...

Q. Definition of Counselling? Counselling has been defined as a process of helping/enabling a person/people solve certain interpersonal (not able to continue relationship), emo

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd