Explain the gram staining technique, Biology

Assignment Help:

Explain the Gram Staining technique?

Gram staining is a differential staining procedure which divides bacteria into two groups: Gram positive and Gram negative, based on staining properties. The gram stain was developed in 1884 by the Danish Physician, Dr. Christian Gram. It is one of the most important and widely employed staining procedure in bacteriology.

It is very useful and is almost essential in identifying an unknown bacteria. The basis of gram staining is the difference in cell wall composition of two types of bacterial cells. Gram positives have thick cell wall, made up of multi-layered peptidoglycan with extensive cross-linking. On the other hand, gram negative cell wall has a very thin peptidoglycan layer surrounded by outer lipid rich outer membrane. There is no outer membrane in case of gram positives. This difference in cell wall leads to decolourization of gram negatives but not gram positives by decolourizing agent i.e., ethanol or acetone.


Related Discussions:- Explain the gram staining technique

Characteristics of metazoa, Characteristics of Metazoa The unicellular...

Characteristics of Metazoa The unicellular protozoans are highly versatile and successful organisms that show remarkable organization and division of labour within the confine

How do you determine net atp production, How do you determine net atp produ...

How do you determine net atp production after complete oxidation to CO2 and H2O using mitochondrial ?-oxidation, the TCA cycle, the mitochondrial electron transport chain and oxida

Medical and first aid, Medical and First Aid - Factors Affecting Occupation...

Medical and First Aid - Factors Affecting Occupational Health Depending on the type of work the industry must make arrangement for readily available medical facility. A nearby

Determine the following term - amniote egg, Determine the following term - ...

Determine the following term - Amniote egg Eggs that shelter the developing embryo in a water-filled sac—the amnion. Characteristic of the amniote animals, which include reptil

Heterotrophs, Collect images of heterotrophs and make a Powerpoint Presenta...

Collect images of heterotrophs and make a Powerpoint Presentation on them

What is carbohydrates, What is Carbohydrates? Carbohydrates : Carbohyd...

What is Carbohydrates? Carbohydrates : Carbohydrates function mainly as immediate sources of energy, stored energy such as starch, and structural components of cells. Carbohyd

Explain the membrane equilibria, Explain the Membrane Equilibria? A sem...

Explain the Membrane Equilibria? A semipermeable membrane used to separate two liquid phases can, in principle, be permeable to certain species and impermeable to others. A mem

How is the nervous system characterized, Q. How is the nervous system chara...

Q. How is the nervous system characterized in beings of the phylum Annelida? How can one compare cephalization in annelids to cephalization in platyhelminthes and nematodes? An

Ciliates - parasitic protozoan, Ciliates - Parasitic Protozoan The onl...

Ciliates - Parasitic Protozoan The only ciliate of medical and veterinary importance is Balantidium coli which inhabit the large intestine of man, apes, monkeys and pigs. B. c

What do you mean by dicoumarol, Q. What is dicoumarol? How does this substa...

Q. What is dicoumarol? How does this substance act in the clotting process and what are some examples of its toxicity? Coumarinic anticoagulants cannot be administered during p

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd