Explain the gram staining technique, Biology

Assignment Help:

Explain the Gram Staining technique?

Gram staining is a differential staining procedure which divides bacteria into two groups: Gram positive and Gram negative, based on staining properties. The gram stain was developed in 1884 by the Danish Physician, Dr. Christian Gram. It is one of the most important and widely employed staining procedure in bacteriology.

It is very useful and is almost essential in identifying an unknown bacteria. The basis of gram staining is the difference in cell wall composition of two types of bacterial cells. Gram positives have thick cell wall, made up of multi-layered peptidoglycan with extensive cross-linking. On the other hand, gram negative cell wall has a very thin peptidoglycan layer surrounded by outer lipid rich outer membrane. There is no outer membrane in case of gram positives. This difference in cell wall leads to decolourization of gram negatives but not gram positives by decolourizing agent i.e., ethanol or acetone.


Related Discussions:- Explain the gram staining technique

Write the meaning of dsme, Q. Write the meaning of DSME? Generally, it ...

Q. Write the meaning of DSME? Generally, it is observed that soon after being diagnosed with diabetes, the patient gets worried or get depressed. This is a stage where the pati

Explain the transport of amino acids, Explain the Transport of Amino Acids?...

Explain the Transport of Amino Acids? More than one transport or carrier system functions in the absorption of amino acids. The active carrier system for neutral amino acids sh

What is intermediate acting insulin, Q. What is Intermediate acting insulin...

Q. What is Intermediate acting insulin? Intermediate acting: This type works more slowly than short acting. It works hardest 8-12 hours after injection and still keeps on worki

Give the causative organism of bacillary dysentery, Give the causative orga...

Give the causative organism and symptoms of Bacillary Dysentery • Bacillary dysentery is caused by  Shigella sp. (Shigella sonnei, S. dysenteraei). Incubation period is

Biota of littoral zone, Biota of Littoral Zone This zone is the shore ...

Biota of Littoral Zone This zone is the shore region of the marine ecosystems and is subject to violence of waves and tides, fluctuation of water level and variability of temp

Food and diet, 3 main ways in which the body uses food

3 main ways in which the body uses food

What are some examples of interspecific competition, What are some examples...

What are some examples of interspecific competition? Examples of interspecific competition are: the dispute between vultures, worms, flies and microorganisms for carrion and th

Pericardium, The heart is enclosed in a membranous sac called the pericardi...

The heart is enclosed in a membranous sac called the pericardium. It has two layers- the fibrous pericardium which is the outer layer and the serous pericardium that lies inside th

Two things that enter the cell for cellular respiration, What are two thing...

What are two things that enter the cell for cellular respiration? Carbohydrate molecules and oxygen enter the cell during cellular respiration.

Explain the term - migraine, Explain the term - Migraine Perhaps the mo...

Explain the term - Migraine Perhaps the most common neurological disorder, migraine afflicts some 5% to 20% of the population at some time in their lives. The World Federation

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd