Explain the gram staining technique, Biology

Assignment Help:

Explain the Gram Staining technique?

Gram staining is a differential staining procedure which divides bacteria into two groups: Gram positive and Gram negative, based on staining properties. The gram stain was developed in 1884 by the Danish Physician, Dr. Christian Gram. It is one of the most important and widely employed staining procedure in bacteriology.

It is very useful and is almost essential in identifying an unknown bacteria. The basis of gram staining is the difference in cell wall composition of two types of bacterial cells. Gram positives have thick cell wall, made up of multi-layered peptidoglycan with extensive cross-linking. On the other hand, gram negative cell wall has a very thin peptidoglycan layer surrounded by outer lipid rich outer membrane. There is no outer membrane in case of gram positives. This difference in cell wall leads to decolourization of gram negatives but not gram positives by decolourizing agent i.e., ethanol or acetone.


Related Discussions:- Explain the gram staining technique

Spring overturn - overturn, Spring overturn - Overturn In spring and e...

Spring overturn - Overturn In spring and early summer season the increased solar radiation melts the ice cover, which, as it attains a temperature of 4° Celsius, becomes dense

How many chromosomes does a human normal haploid cell have, Q. How many chr...

Q. How many chromosomes does a human normal diploid cell have? How many chromosomes does a human normal haploid cell have? How many are the sex chromosomes within each of them?

Morphology of angiosperms, define inflorescence and explain in detail about...

define inflorescence and explain in detail about it''s types

What do you mean by salmonellosis, Salmonellosis is the most reported zoono...

Salmonellosis is the most reported zoonotic disease in European countries. Salmonellosis (Salmonella gastroenteritis) results from the ingestion of foods that contain significant n

Sensor measures the current value of the controlled variable, In a properly...

In a properly functioning negative feedback system A. value of the controlled variable will always be very close to the threshold value when the system is in steady state.

#title.humans and environment., what are the two main pollutants that contr...

what are the two main pollutants that contribute to aci rain

What are the elements that form the middle ear, Q. What are the elements th...

Q. What are the elements that form the middle ear? What are the names of the three middle ear ossicles that participate in the phonosensitivity? The middle ear is formed by the

Industrial melanism, In this section, we shall discuss a classic example of...

In this section, we shall discuss a classic example of natulal selection in action. In the preceding unit, it was stated that natural selection always aims at eliminating alleles,

Explain the uses of isp in seafood products, Explain the Uses of ISO in Sea...

Explain the Uses of ISO in Seafood products  Seafood products  The most significant application in this category is the use of ISP in fish sausage and surimi based restructu

#title.examples of classes of vertebrates, how to explain an example from e...

how to explain an example from each class of vertebrate

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd