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Explain the Gram Staining technique?
Gram staining is a differential staining procedure which divides bacteria into two groups: Gram positive and Gram negative, based on staining properties. The gram stain was developed in 1884 by the Danish Physician, Dr. Christian Gram. It is one of the most important and widely employed staining procedure in bacteriology.
It is very useful and is almost essential in identifying an unknown bacteria. The basis of gram staining is the difference in cell wall composition of two types of bacterial cells. Gram positives have thick cell wall, made up of multi-layered peptidoglycan with extensive cross-linking. On the other hand, gram negative cell wall has a very thin peptidoglycan layer surrounded by outer lipid rich outer membrane. There is no outer membrane in case of gram positives. This difference in cell wall leads to decolourization of gram negatives but not gram positives by decolourizing agent i.e., ethanol or acetone.
Kinds of Hermaphroditism Depending upon the timing of maturity of the gonads, hermaphroditism can be of two types 1. Simultaneous hermaphroditism . Both male and female g
Describe Class Diplopoda and Chilopoda in detail? Members of the Subphylum Crustacea and the Subphylum Uniramia have one major characteristic in common. Both groups have biting
A new drug named AGON-CaSR has been developed that is an agonist at calcium-binding sites of CaSRs (Calcium-Sensing Receptors) in plasma membranes of parathyroid gland cells.
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Tonicity and the plant cell. Table 3: Amount of water displaced by potato samples before and after experiment?
When a ventricQlar septa1 defect (VSD) is present, with no aortic stenosis, the peak velocitgr across the defect reflects the difference in pressure between the right ventricle an
Conduct of Perfusion : At the beginning of the bypass, the pump output is usually kept at 2.4 litres per meter per minute. On coming off bypass, the flow is gradually reduced til
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