Explain the gram staining technique, Biology

Assignment Help:

Explain the Gram Staining technique?

Gram staining is a differential staining procedure which divides bacteria into two groups: Gram positive and Gram negative, based on staining properties. The gram stain was developed in 1884 by the Danish Physician, Dr. Christian Gram. It is one of the most important and widely employed staining procedure in bacteriology.

It is very useful and is almost essential in identifying an unknown bacteria. The basis of gram staining is the difference in cell wall composition of two types of bacterial cells. Gram positives have thick cell wall, made up of multi-layered peptidoglycan with extensive cross-linking. On the other hand, gram negative cell wall has a very thin peptidoglycan layer surrounded by outer lipid rich outer membrane. There is no outer membrane in case of gram positives. This difference in cell wall leads to decolourization of gram negatives but not gram positives by decolourizing agent i.e., ethanol or acetone.


Related Discussions:- Explain the gram staining technique

What are the major harms caused by vitamin a deficiency, Q. What are the ma...

Q. What are the major harms caused by vitamin A deficiency? How does this vitamin act in the physiology of vision? Deficiency of vitamin A (retinol) may corneal dryness (xeroph

Define the clinical manifestations of underweight, Define the Clinical Mani...

Define the Clinical Manifestations of Underweight? Underweight may predispose to fatigue, lethargy and breathlessness. Iron-deficiency anaemia is usually seen because the diet

Why epiphyseal closure differs between males and females, It is said during...

It is said during puberty rising sex hormones stimulate osteoblasts to produce bone faster than the rate at which epiphyseal cartilage expands. The timing of epiphyseal closure dif

Nutritional management in inflammatory bowel disease, Q. Nutritional Manage...

Q. Nutritional Management in Inflammatory Bowel Disease? Adequacy of nutritional needs and minimizing stress on the inflamed or narrowed segment of the bowel are the main princ

Availability of adp, Availability of ADP When  the ADP  levels increase...

Availability of ADP When  the ADP  levels increase due to hydrolysis ofATP  in various biosynthetic reactions,  the  rate of reaction to generate ATP  is accelerated and this i

Explain about the chromium metabolism, Explain about the Chromium Metabolis...

Explain about the Chromium Metabolism? Chromium appears to be absorbed throughout the small intestine, with absorption being higher in jejunum. The mechanism of absorption has

What are some examples of parasitism, What are some examples of parasitism?...

What are some examples of parasitism? Classical instances are the parasites of humans (host), as the trypanosome that causes Chagas' disease, the HIV virus (AIDS), the bacteria

Explain the dark adaptation of vitamin a, Explain the Dark Adaptation of Vi...

Explain the Dark Adaptation of Vitamin A? In the early stages of VAD, the individual cannot see objects in dim light. This' phenomenon is used as a criterion for assessment in

Nursing assessment of acute renal failure, Nursing Assessment If the ...

Nursing Assessment If the cause is due to hypovolemia you may observe the child for dry mucus membrane, poor skin turger, hypotension and oliguria. You should also observe

Define the factors that affect the rate of drying, Define the Factors that ...

Define the Factors that Affect the Rate of Drying? Factors that affect the rate of drying are as follow: 1) The fat content of the food-Higher fat contents generally result

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd