Explain the gram staining technique, Biology

Assignment Help:

Explain the Gram Staining technique?

Gram staining is a differential staining procedure which divides bacteria into two groups: Gram positive and Gram negative, based on staining properties. The gram stain was developed in 1884 by the Danish Physician, Dr. Christian Gram. It is one of the most important and widely employed staining procedure in bacteriology.

It is very useful and is almost essential in identifying an unknown bacteria. The basis of gram staining is the difference in cell wall composition of two types of bacterial cells. Gram positives have thick cell wall, made up of multi-layered peptidoglycan with extensive cross-linking. On the other hand, gram negative cell wall has a very thin peptidoglycan layer surrounded by outer lipid rich outer membrane. There is no outer membrane in case of gram positives. This difference in cell wall leads to decolourization of gram negatives but not gram positives by decolourizing agent i.e., ethanol or acetone.


Related Discussions:- Explain the gram staining technique

Explain the regulation of breathing and blood pressure, Which is the brain ...

Which is the brain region responsible for the regulation of breathing and blood pressure? The neural regulation of breathing, blood pressure and other physiological parameters

Define procedure for testing the presence of urea in milk, Define Procedure...

Define Procedure for Testing the Presence of Urea in Milk? 1. To 0.5 ml of milk add 0.5 ml of  DMAB solution  (prepared by dissolving 0.4 gm of p-dimethyl amino benzaldehyde po

Definition of probiotics and prebzotics, Definition of probiotics and prebz...

Definition of probiotics and prebzotics? An early definition of probiotics is 'organisms and substances which contribute to intestinal microbial balance'. Later the word 'subst

Define cancer - excessive fat intake, Define Cancer - excessive fat intake?...

Define Cancer - excessive fat intake? Role of fat in cancer is debatable. High fat intake is indicated as a risk factor to cancer of breast, colon etc. Saturated fat intake has

Determine the different types of sensory receptors, Determine the different...

Determine the different types of sensory receptors There are different types of sensory receptors as follows:  Visual receptors in the eyes for vision.  Auditory receptor

What is a biosphere?, What is a biosphere? A biosphere is a set of all ...

What is a biosphere? A biosphere is a set of all of the ecosystems of the planet.

Describe about mitochondria and how they are associated with, Mitochondria,...

Mitochondria, which fuel life's processes in all cells, uses nutrients from digested food and oxygen which has diffused across cell surface. In freshwater protozoans water expulsio

Extracted chlorophyll pigments, Extracted chlorophyll pigments If we...

Extracted chlorophyll pigments If we take chlorophyll molecule in a test tube and provide 2 CO and water and expose the mixture to sunlight, glucose is not formed. If we

Exercise and medications in bronchial asthma, Exercise Stress or em...

Exercise Stress or emotional upset  Medications Aspirin, non-steroidal anti-inflammatory drugs(NSAIDs), beta- blockers(including eye drops), cholinergic dr

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd