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Explain the Gram Staining technique?
Gram staining is a differential staining procedure which divides bacteria into two groups: Gram positive and Gram negative, based on staining properties. The gram stain was developed in 1884 by the Danish Physician, Dr. Christian Gram. It is one of the most important and widely employed staining procedure in bacteriology.
It is very useful and is almost essential in identifying an unknown bacteria. The basis of gram staining is the difference in cell wall composition of two types of bacterial cells. Gram positives have thick cell wall, made up of multi-layered peptidoglycan with extensive cross-linking. On the other hand, gram negative cell wall has a very thin peptidoglycan layer surrounded by outer lipid rich outer membrane. There is no outer membrane in case of gram positives. This difference in cell wall leads to decolourization of gram negatives but not gram positives by decolourizing agent i.e., ethanol or acetone.
Summer Stratification - Thermal stratification Thermal stratification is fairly pronounced during the summer seasons in most lakes of the temperate (cold) regions but is rare
Cells are classified into eukaryotic or prokaryotic. Prokaryotic cell is that with no a delimited nucleus. Eukaryotic cells are those with nucleus delimited by membrane.
Protein Classification Based on Shape There are three types of proteins on the basis of shape - Fibrous, Globular, Intermediate 1 . FIBROUS PROTEINS They ar
Implant Selection Errors There are a plethora of commercially available implant systems and designs. Qualitative and quantitative nature of the available bone should be careful
Results: Early mortality for tricuspid valve replacement is around six per cent. But depending on the patient's class of symptoms and the number of concomitant procedures, this c
Define Essentiality of Phosphorus for Growth of Micro-Organism Phosphorus is used in the form of phosphate salt by microorganisms. It is involved in formation of nucleic acids,
List the goals of targeting blood pressure in diabetics. Goals of targeting blood pressure in diabetics are: Patients with diabetes should be treated to a systolic blood pr
How are fruits formed? The fecundation in angiosperms triggers the release of hormones that act upon the ovaries. The ovary wall then makes into a fruit that having the seeds.
Q. Why is a leguminous crop rotation used in agriculture? The Leguminous crop rotation and other crop rotations are used in agriculture for the reason that in these plants many
Clinical symptoms of typhoid are: 1. Graded fever which follows an upward ladder pattern. 2. Abdominal pain, cramps and diarrhoea. 3. Anorexia and vomiting. 4. In
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