Explain the gram staining technique, Biology

Assignment Help:

Explain the Gram Staining technique?

Gram staining is a differential staining procedure which divides bacteria into two groups: Gram positive and Gram negative, based on staining properties. The gram stain was developed in 1884 by the Danish Physician, Dr. Christian Gram. It is one of the most important and widely employed staining procedure in bacteriology.

It is very useful and is almost essential in identifying an unknown bacteria. The basis of gram staining is the difference in cell wall composition of two types of bacterial cells. Gram positives have thick cell wall, made up of multi-layered peptidoglycan with extensive cross-linking. On the other hand, gram negative cell wall has a very thin peptidoglycan layer surrounded by outer lipid rich outer membrane. There is no outer membrane in case of gram positives. This difference in cell wall leads to decolourization of gram negatives but not gram positives by decolourizing agent i.e., ethanol or acetone.


Related Discussions:- Explain the gram staining technique

Mode of nutrition, What is the mode of nutrition of Ascaris?

What is the mode of nutrition of Ascaris?

Suppose that the substance does not ionize, Percent solutions are made on t...

Percent solutions are made on the basis of g/ml or ml/ml of diluent, and it is usually assumed that 1 ml of water = 1 g of water. For example, 20 g/100 ml of water or 200 g/1000 ml

Chemical properties of fatty acids, Chemical Properties of Fatty Acids ...

Chemical Properties of Fatty Acids Esterification Like any other organic acid,  fatty acids also form esters with  various alcohols. An alcohol such as glycerol is reacted

What is phenotypical proportion, Considering hybridization in a given trait...

Considering hybridization in a given trait like the color of the hair of a mammalian species (white/black) conditioned by a pair of different alleles under complete dominance (blac

Define nutritional requirements of fats and oils for adults, Define Nutriti...

Define Nutritional Requirements of Fats and Oils for Adults? A desirable amount of a linoleic acid to be consumed by a normal adult is 3 en% (ICMR, 1990). The invisible fat pre

Mass extinction, Mas s Extinction - Since beginning many species ev...

Mas s Extinction - Since beginning many species evolved and got extinct (in fact only 1 % is living). Sometimes it occured suddenly at large scale, (most recently dinosa

Can obesity causes the cardiovascular disease, Q. Can Obesity causes the ca...

Q. Can Obesity causes the cardiovascular disease? Obesity is established as a leading predictor of CAD and is associated with several cardiovascular risk factors like cholester

Global alteration of brain functioning - sleep, Q. Global alteration of bra...

Q. Global alteration of brain functioning - Sleep? Sleep involves a global alteration of brain functioning, and occupies one third of our lives, The transition from waking to

Explain glycogenolysis, Glycogenolysis Unlike glycogenesis, glycogenoly...

Glycogenolysis Unlike glycogenesis, glycogenolysis is the  breakdown of glycogen. Glycogen is  broken down  in  the  liver and muscle catalysed  by  the  enzyme glycogen phosph

What are the types of colloidal dispersions, What are the types of colloida...

What are the types of colloidal dispersions Depending upon the relative affinity of the dispersed phase for the dispersion medium, colloidal dispersions are, further divided in

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd