Explain the gram staining technique, Biology

Assignment Help:

Explain the Gram Staining technique?

Gram staining is a differential staining procedure which divides bacteria into two groups: Gram positive and Gram negative, based on staining properties. The gram stain was developed in 1884 by the Danish Physician, Dr. Christian Gram. It is one of the most important and widely employed staining procedure in bacteriology.

It is very useful and is almost essential in identifying an unknown bacteria. The basis of gram staining is the difference in cell wall composition of two types of bacterial cells. Gram positives have thick cell wall, made up of multi-layered peptidoglycan with extensive cross-linking. On the other hand, gram negative cell wall has a very thin peptidoglycan layer surrounded by outer lipid rich outer membrane. There is no outer membrane in case of gram positives. This difference in cell wall leads to decolourization of gram negatives but not gram positives by decolourizing agent i.e., ethanol or acetone.


Related Discussions:- Explain the gram staining technique

Can you explain salmonellosis, Q. Can you explain Salmonellosis Salmonel...

Q. Can you explain Salmonellosis Salmonellosis is a common food bome disease all over the world. There are approximately 1600 different strains (sero types) of Salmonell

Mixed mitral stenosis and regurgitation, Mixed Mitral Stenosis and Regurgit...

Mixed Mitral Stenosis and Regurgitation The most common cause for a combined lesion is rheumatic, Very rarely; it could be of congenital origin. Regurgitation in a stenotic val

Difference between anaerobic and aerobic beings, Q. What is the difference ...

Q. What is the difference between anaerobic and aerobic beings? Anaerobic organisms are those that live or can live under oxygen-lacking environments and Aerobic organisms are

Explain the nutrient requirements during lactation, Normal 0 fa...

Normal 0 false false false EN-IN X-NONE X-NONE

Precautions for determination of fungal and yeast count, Explain Precaution...

Explain Precautions for Determination of Fungal and Yeast Count? 1. Maintain aseptic conditions. 2. Keep alcohol away from flame. 3. To avoid infections and allergies, ca

Use of nitrates and nitrites in microorganisms, Q. Explain the Use of nitra...

Q. Explain the Use of nitrates and nitrites in microorganisms? The use of nitrates and nitrites has been widely used in the curing of meat. The reduction of nitrates by bacteri

Determine about the accommodation of eye, Determine about the Accommodation...

Determine about the Accommodation of eye Accommodation is the ability of the overall refracting power of the eye to change to clearly view objects at different distances. This

What are tannins, What are Tannins? Long known for their inhibitory eff...

What are Tannins? Long known for their inhibitory effects on iron absorption, recent research indicates that tannins do have beneficial effects. Tannins are compounds of high m

Stomatal resistance, Stomatal Resistance Stomatal resistance is most i...

Stomatal Resistance Stomatal resistance is most important because gas exchange between leaves and external atmosphere takes place entirely through stomatal pores. Stomatal res

Define magnesium levels in erythrocytes and lymphocytes, Define Magnesium l...

Define Magnesium levels in erythrocytes and lymphocytes? Magnesium levels in erythrocytes and lymphocytes: These measures appear to provide a more accurate assessment of body M

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd