Explain the gram staining technique, Biology

Assignment Help:

Explain the Gram Staining technique?

Gram staining is a differential staining procedure which divides bacteria into two groups: Gram positive and Gram negative, based on staining properties. The gram stain was developed in 1884 by the Danish Physician, Dr. Christian Gram. It is one of the most important and widely employed staining procedure in bacteriology.

It is very useful and is almost essential in identifying an unknown bacteria. The basis of gram staining is the difference in cell wall composition of two types of bacterial cells. Gram positives have thick cell wall, made up of multi-layered peptidoglycan with extensive cross-linking. On the other hand, gram negative cell wall has a very thin peptidoglycan layer surrounded by outer lipid rich outer membrane. There is no outer membrane in case of gram positives. This difference in cell wall leads to decolourization of gram negatives but not gram positives by decolourizing agent i.e., ethanol or acetone.


Related Discussions:- Explain the gram staining technique

Describe the ways in which a genome can be sequenced, 1. Describe what effe...

1. Describe what effects epigenetic modifications such as methylation and acetylation can have in eukaryotic cells, and how these modifications are added or removed from chromatin.

Obtain half their genes from one parent, If children obtain half their gene...

If children obtain half their genes from one parent and half from the other parent, why aren't siblings identical?

Determine the strengths of full battery measures, Determine the Strengths o...

Determine the Strengths of Full Battery Measures 1) Wide range of scores allow differential diagnosis in various cognitive disorders 2) More reliable, and sensitive 3) Br

Explain the recommended dietary allowance for nicotinic acid, Explain the R...

Explain the Recommended Dietary Allowance for nicotinic acid? There are various factors and which niacin requirements depend. These are energy Utilization, body size and dietar

What is the basic role of botanic garden, Q. What is the basic Role of Bota...

Q. What is the basic Role of Botanic Garden? A botanic garden makes us to simply enjoy the beauty and variety of nature. The pleasure of admiring turns us into good naturalists

Explain seed gums, Seed gums Polysaccharides derived from plants and se...

Seed gums Polysaccharides derived from plants and seeds have been in use for thousand of years. Seed gums are a group of polysaccharides with the following properties: S

Cleavage, what are the chemical changes during cleavage

what are the chemical changes during cleavage

Hunting and export - wildlife, Hunting and Export - Wildlife The hunti...

Hunting and Export - Wildlife The hunting and export of excessive numbers of certain animal species is another important factor leading to dangerous reductions in numbers. The

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd