Explain the gram staining technique, Biology

Assignment Help:

Explain the Gram Staining technique?

Gram staining is a differential staining procedure which divides bacteria into two groups: Gram positive and Gram negative, based on staining properties. The gram stain was developed in 1884 by the Danish Physician, Dr. Christian Gram. It is one of the most important and widely employed staining procedure in bacteriology.

It is very useful and is almost essential in identifying an unknown bacteria. The basis of gram staining is the difference in cell wall composition of two types of bacterial cells. Gram positives have thick cell wall, made up of multi-layered peptidoglycan with extensive cross-linking. On the other hand, gram negative cell wall has a very thin peptidoglycan layer surrounded by outer lipid rich outer membrane. There is no outer membrane in case of gram positives. This difference in cell wall leads to decolourization of gram negatives but not gram positives by decolourizing agent i.e., ethanol or acetone.


Related Discussions:- Explain the gram staining technique

Biota of the neritic oceanic zone, Biota of the Neritic Oceanic Zone T...

Biota of the Neritic Oceanic Zone This zone constitutes 75 per cent of the total oceanic area and is relatively rich in species and high in productivity owing to factors such

How to removes hydrogen from fatty acids, Suppose you treated butter with a...

Suppose you treated butter with a fatty acid desaturase, an enzyme that removes hydrogen from fatty acids and creates double bonds. What would happen?

Show the communication process steps, Q. Show the Communication process ste...

Q. Show the Communication process steps? Planning - Set goals. - Make a plan with alternatives. - Allow the counsellee to select a alternative which is feasible.

Physical lay out, Physical Lay Out: Physical lay out of the nursing un...

Physical Lay Out: Physical lay out of the nursing unit should be planned and built in such a manner so that it helps the head nurse to discharge her duties/carry  out her resp

Define a tumbler garden, A tumbler garden Grow several kinds of seeds i...

A tumbler garden Grow several kinds of seeds in 'tumbler gardens'. Every pupil might grow a tumbler garden of his own and keep a day by day pictorial record of the progress of

Explain phylum phytofagellata, Phylum Phytofagellata (Plant flagellates...

Phylum Phytofagellata (Plant flagellates) 1) At some stage of life cycle they have one or more flagella. The flagella are used for locomotion. 2) Photosynthetic protistan

What is mdrtb, What is MDRTB MDRTB - For patients with known exposure t...

What is MDRTB MDRTB - For patients with known exposure to multi-drug resistant TB (MDRTB) and a high risk of developing active TB, there are no data-based recommendations. Regi

Types of ecosystem, Ecosystems are so varied in form and structure that wha...

Ecosystems are so varied in form and structure that whatever has a distinct community of its own, can be called an ecosystem eg. A crop field, grass land, barks etc. all the ecosys

What is the first polar body, Q. What is the first polar body? How differen...

Q. What is the first polar body? How different is it from the oocyte II? In oogenesis the oogonium differentiates into oocyte I (2n) and this cell enters meiosis and after fini

Define single beam and double beam optical path, Define Single Beam and Dou...

Define Single Beam and Double Beam Optical Path? In simpler instruments, a single beam of light passes through the cuvettes and reaches the detector whereas in the double beam

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd