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Explain the Gram Staining technique?
Gram staining is a differential staining procedure which divides bacteria into two groups: Gram positive and Gram negative, based on staining properties. The gram stain was developed in 1884 by the Danish Physician, Dr. Christian Gram. It is one of the most important and widely employed staining procedure in bacteriology.
It is very useful and is almost essential in identifying an unknown bacteria. The basis of gram staining is the difference in cell wall composition of two types of bacterial cells. Gram positives have thick cell wall, made up of multi-layered peptidoglycan with extensive cross-linking. On the other hand, gram negative cell wall has a very thin peptidoglycan layer surrounded by outer lipid rich outer membrane. There is no outer membrane in case of gram positives. This difference in cell wall leads to decolourization of gram negatives but not gram positives by decolourizing agent i.e., ethanol or acetone.
Active Transport We have mentioned above that Transmembrane potential develops due to active transport of ions (H + ) outside the cell. Since this transport takes place again&
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A leaf is detached from a tree and tested with iodine. The leaf turns dark blue. (a) What does this result tell you? (b) Why is this result not sufficient e
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Explain about the Soil morphology Soil morphology is the description of the soil body and its general characteristics. The morphology of the soil is expressed by number, kinds
various changes that occur during concentration of food
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