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Explain the Gram Staining technique?
Gram staining is a differential staining procedure which divides bacteria into two groups: Gram positive and Gram negative, based on staining properties. The gram stain was developed in 1884 by the Danish Physician, Dr. Christian Gram. It is one of the most important and widely employed staining procedure in bacteriology.
It is very useful and is almost essential in identifying an unknown bacteria. The basis of gram staining is the difference in cell wall composition of two types of bacterial cells. Gram positives have thick cell wall, made up of multi-layered peptidoglycan with extensive cross-linking. On the other hand, gram negative cell wall has a very thin peptidoglycan layer surrounded by outer lipid rich outer membrane. There is no outer membrane in case of gram positives. This difference in cell wall leads to decolourization of gram negatives but not gram positives by decolourizing agent i.e., ethanol or acetone.
Explain Oxygen Therapyin respiratory support? Oxygen is given to correct hypoxemia. An initial high concentration of oxygen (FiO2) is given while the underlying cause is identi
The biosphere is composed of all living organism. This sphere includes all the microorganism, plants and animals of earth. In fact biosphere involves interaction of living beings w
what is the classification of skeletal tissue?
Steps of C dna amplification 1. Smart-pcr amplification of cdna is the technique which initiates with the change of mrna to cdna utilizing mmlv-rt, mutated in the rnase h dom
Define Vitamin K-dependent carboxylation? Vitamin K acts as a cofactor in the synthesis of g-carboxyglutamic acid (Gla) from glutamic acid residues required for the normal coa
Enzymes work to speed up a chemical reaction by: a. attaching coenzymes to the substrate b. heating up the substrate c. lowering the activation energy of the reaction d. supplyi
Phylum Coelentrat a : (9,000 'species) The body is radial and made up of two cell layers. Gastro-Vascular cavity is saclike, tentacles and stinging cells are present. They may
Types of Amoeboid Movements As the amoeba's cell body throws out one or a few pseudopodial lobes, a temporary rear end or uroid is pulled along. The central, more fluid protop
what type of skull does amphibians possess in general?
G L YCOLIPIDS They are lipids having sugar residues. Two common glycolipids are cerebrosides and gangliosides. (1) Cerebrosides They are made of amino alcohol sphi
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