Explain the gram staining technique, Biology

Assignment Help:

Explain the Gram Staining technique?

Gram staining is a differential staining procedure which divides bacteria into two groups: Gram positive and Gram negative, based on staining properties. The gram stain was developed in 1884 by the Danish Physician, Dr. Christian Gram. It is one of the most important and widely employed staining procedure in bacteriology.

It is very useful and is almost essential in identifying an unknown bacteria. The basis of gram staining is the difference in cell wall composition of two types of bacterial cells. Gram positives have thick cell wall, made up of multi-layered peptidoglycan with extensive cross-linking. On the other hand, gram negative cell wall has a very thin peptidoglycan layer surrounded by outer lipid rich outer membrane. There is no outer membrane in case of gram positives. This difference in cell wall leads to decolourization of gram negatives but not gram positives by decolourizing agent i.e., ethanol or acetone.


Related Discussions:- Explain the gram staining technique

Why the vast majority of segregation errors in human patient, Which of the ...

Which of the following defines why the vast majority of segregation errors in human patients include the two sex chromosomes (XXY, XO, XYY) or chromosome 21 (triple 21)? A. Th

What are the complementary base-pairing rules for biology, What are the com...

What are the complementary base-pairing rules for biology? In DNA, Adenine bonds with Thymine, Cytosine bonds with Guanine. In RNA, Thymine is changed with Uracil (bases capita

How vitamin e is directly related to vitamins a, How vitamin e is directly ...

How vitamin e is directly related to Vitamins A, D and K? A high intake of vitamin E interferes with the functions of other fat-soluble vitamins such as vitamin K absorpt

Methods of filter feeding, Methods of Filter Feeding More elaborate me...

Methods of Filter Feeding More elaborate methods of filter feeding are seen in tube dwelling polychaetes which use tentacles to entangle the food particles. Figure shows some

Explain how movement is controlled in protozoans., Movement is even control...

Movement is even controlled by organelles, cilia and flagella. These and the pseudopodia in the amoebas give these small creatures movement, something we associate with animals. Es

What is the salivary digestive enzyme, Q. What is the salivary digestive en...

Q. What is the salivary digestive enzyme? Which type of food does it digest and into which smaller molecules does it transform the food? The salivary hydrolase is known as sali

Determine the concept of imitation in the learning process, Determine the c...

Determine the concept of  imitation in the learning process The concept of  imitation in the learning process is also a key developmental concept for infants and young children

In which organ red blood cells are stored, Surplus red blood cells, require...

Surplus red blood cells, required to meet an emergency, are generally stored in what organ of the human body? Is it the: a) Liver b) Spleen c) pancreas d) Kidneys

Applications of oracle clinical in pharmaceutical industries, Question 1: ...

Question 1: Write the applications of Oracle clinical in Pharmaceutical industries Explain what is "Oracle clinical" Explain different application of oracle clinical

How many cells are in the human body, How many cells are in the human body?...

How many cells are in the human body? According to "The Handy Science Answer Book" noted by the Science and Technology Department of the Carnegie Library of Pittsburgh (1994) th

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd