Explain the gram staining technique, Biology

Assignment Help:

Explain the Gram Staining technique?

Gram staining is a differential staining procedure which divides bacteria into two groups: Gram positive and Gram negative, based on staining properties. The gram stain was developed in 1884 by the Danish Physician, Dr. Christian Gram. It is one of the most important and widely employed staining procedure in bacteriology.

It is very useful and is almost essential in identifying an unknown bacteria. The basis of gram staining is the difference in cell wall composition of two types of bacterial cells. Gram positives have thick cell wall, made up of multi-layered peptidoglycan with extensive cross-linking. On the other hand, gram negative cell wall has a very thin peptidoglycan layer surrounded by outer lipid rich outer membrane. There is no outer membrane in case of gram positives. This difference in cell wall leads to decolourization of gram negatives but not gram positives by decolourizing agent i.e., ethanol or acetone.


Related Discussions:- Explain the gram staining technique

Spermiogenesis - spermatogenesis, Spermiogenesis - Spermatogenesis At ...

Spermiogenesis - Spermatogenesis At the end of the meiosis the spermatids appear as simple spherical cells with a centrally located nucleus. Their differentiation into sperm r

Shows evidence to see the in environment abd ecology, Shows evidence of the...

Shows evidence of the ability to see the interaction between environment / ecology and genetics /  evolution Some candidates may recognise that evolution can result in less no

Explain the predator-prey relationships, Explain the Predator-Prey Relation...

Explain the Predator-Prey Relationships ? The feeding by a population of one species upon members of another species is referred to as predation. Many scientists also consider

Agriculture - degradation of ecosystem, Agriculture - Degradation of Ecosys...

Agriculture - Degradation of Ecosystem Agriculture whether traditional or modern involves intervention and modification of the natural ecological systems. Ever since man has s

Week 5 Review Sheet I, How does true motility differ from brownian movement...

How does true motility differ from brownian movement? What morphological structre is responsible for bacteria motility? Why is the wet preparation discarded in disinfectant s

What are the functions of insulin and glucagon, What are the functions of i...

What are the functions of insulin and glucagon for the blood glucose control? Glucagon enhances glycemia and insulin reduces it. They are antagonistic pancreatic hormones. Gluc

Explain the spermatogonium and spermatocyte I, What is the difference betwe...

What is the difference between spermatogonium and spermatocyte I? The male germ cells are the spermatogonia (diploid cells, 2n) located in the testicles. They mature and by mea

What are the factors that for influencing photosynthesis, What are the fact...

What are the factors that for influencing photosynthesis also interfere with the gross primary productivity? Mostly water and light, but also mineral salts, temperature and car

Agro industrial-enrichment of soil, Enrichment of soil Indirect provis...

Enrichment of soil Indirect provision of minerals to grazing livestock includes mineral fertilization of  pasture and altering soil pH, however this may not be always feasible

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd