Explain the gram staining technique, Biology

Assignment Help:

Explain the Gram Staining technique?

Gram staining is a differential staining procedure which divides bacteria into two groups: Gram positive and Gram negative, based on staining properties. The gram stain was developed in 1884 by the Danish Physician, Dr. Christian Gram. It is one of the most important and widely employed staining procedure in bacteriology.

It is very useful and is almost essential in identifying an unknown bacteria. The basis of gram staining is the difference in cell wall composition of two types of bacterial cells. Gram positives have thick cell wall, made up of multi-layered peptidoglycan with extensive cross-linking. On the other hand, gram negative cell wall has a very thin peptidoglycan layer surrounded by outer lipid rich outer membrane. There is no outer membrane in case of gram positives. This difference in cell wall leads to decolourization of gram negatives but not gram positives by decolourizing agent i.e., ethanol or acetone.


Related Discussions:- Explain the gram staining technique

Define defensive proteins - biological functions of proteins, Define Defens...

Define Defensive Proteins - Biological Functions of Proteins? These proteins protect in opposition to diseases. Antibodies are the instance of defensive proteins. These combat

What is a membrane, What is a membrane? Membrane is any delicate sheet ...

What is a membrane? Membrane is any delicate sheet that divides one region from another blocking or permitting (selectively or completely) the passage of substances. The skin,

What is the importance of fungi, What is the Importance of Fungi? Fungi...

What is the Importance of Fungi? Fungi are widely distributed in nature. It is estimated that there are about 1.5 million species of fungi on earth. Fungi are both beneficial a

How sugar play a major role in fermentation, How sugar play a major role in...

How sugar play a major role in Fermentation? The production of chemicals by fermenting several sugars is a very well-accepted science. Its utilize ranges from producing beverag

What are the chemical substances formed by water photolysis, What are the c...

What are the chemical substances formed by water photolysis? What is the destination of each of those substances? Free electrons, hydrogen ions and molecular oxygen are liberat

Determine the diversity of biological evolution, Is crossing over important...

Is crossing over important for the diversity of biological evolution? Sexual reproduction and recombination of linked genes (crossing over) are, with mutations, the main instru

Do we know what causes mental illnesses or how to treat them, Do we know wh...

Do we know what causes mental illnesses, or how best to treat them? Mental illnesses take various forms: debilitating sadness in depression; uncontrollable repetitive actions i

General process of post-hatching growth in insects, General process of post...

General process of post-hatching growth in insects While a young insect hatches out from an egg it is covered by a firm, inflexible, sclerotized cuticle, which because of its

Uricotelism, Normal 0 false false false EN-IN X-NONE ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

What are coacervates, What are coacervates? Coarcervates are small stru...

What are coacervates? Coarcervates are small structures made of the aggregation of organic molecules under water solution. By electrical attraction the molecules join into bigg

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd