Explain the gram staining technique, Biology

Assignment Help:

Explain the Gram Staining technique?

Gram staining is a differential staining procedure which divides bacteria into two groups: Gram positive and Gram negative, based on staining properties. The gram stain was developed in 1884 by the Danish Physician, Dr. Christian Gram. It is one of the most important and widely employed staining procedure in bacteriology.

It is very useful and is almost essential in identifying an unknown bacteria. The basis of gram staining is the difference in cell wall composition of two types of bacterial cells. Gram positives have thick cell wall, made up of multi-layered peptidoglycan with extensive cross-linking. On the other hand, gram negative cell wall has a very thin peptidoglycan layer surrounded by outer lipid rich outer membrane. There is no outer membrane in case of gram positives. This difference in cell wall leads to decolourization of gram negatives but not gram positives by decolourizing agent i.e., ethanol or acetone.


Related Discussions:- Explain the gram staining technique

Biodeterioration, is paper broken down by microorganisms

is paper broken down by microorganisms

Define the beer''s law - nutritional biochemistry, Define the Beer's Law -...

Define the Beer's Law - Nutritional  Biochemistry? Beer's law states that the concentration of a substance is directly proportional to the amount of light absorbed or inversely

What is an erlenmeyer flask and how is it used, What is an Erlenmeyer flask...

What is an Erlenmeyer flask and how is it used? It is same to a beaker, except that it gets narrower at the top. It is use to hold liquids and do reactions in it.

What carbohydrate characteristic chemical features include, Carbohydrate ch...

Carbohydrate characteristic chemical features include: Select one: a. the potential to form multiple hydrogen bonds. b. the existence of one or more asymmetric centers.

What are the main degenerative diseases of the heart, What are the main deg...

What are the main degenerative diseases of the heart? The major degenerative diseases of the heart are heart failure, arrhythmias, valvular heart diseases, coronary insufficie

Explain the objectives of nutritional care, Explain the Objectives of nutri...

Explain the Objectives of nutritional care? - To minimize the development of nutrient imbalance. - To maintain fluid and electrolyte homeostasis - To promote energy equil

Explain the principle of nelson-somogyi method, Explain the Principle of Ne...

Explain the Principle of Nelson-Somogyi Method? Glucose is estimated by Nelson-Somogyi method. We begin our study of this method by getting to know the principle involved in th

Explain the body tube of microscope, Explain the Body Tube of Microscope? ...

Explain the Body Tube of Microscope? Above the stage and attached to the arm is the body tube, to which eye piece(s) or ocular lens and revolving nosepiece is attached. Microsc

Domestic effluents - causes of water pollution, Domestic Effluents - Causes...

Domestic Effluents - Causes of Water Pollution Liquid waste produced daily in kitchen, bathroom and toilet are discharged directly or through canal, river or any other water b

Foot examination for an diabetics, Foot examination For all diabetics, ...

Foot examination For all diabetics, foot examination is important and necessary in all visits to identify risk factors of ulcers or injury of foot. This will help in preventing

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd