Explain the gram staining technique, Biology

Assignment Help:

Explain the Gram Staining technique?

Gram staining is a differential staining procedure which divides bacteria into two groups: Gram positive and Gram negative, based on staining properties. The gram stain was developed in 1884 by the Danish Physician, Dr. Christian Gram. It is one of the most important and widely employed staining procedure in bacteriology.

It is very useful and is almost essential in identifying an unknown bacteria. The basis of gram staining is the difference in cell wall composition of two types of bacterial cells. Gram positives have thick cell wall, made up of multi-layered peptidoglycan with extensive cross-linking. On the other hand, gram negative cell wall has a very thin peptidoglycan layer surrounded by outer lipid rich outer membrane. There is no outer membrane in case of gram positives. This difference in cell wall leads to decolourization of gram negatives but not gram positives by decolourizing agent i.e., ethanol or acetone.


Related Discussions:- Explain the gram staining technique

Mechanical valves, Mechanical Valves They are made of a combination o...

Mechanical Valves They are made of a combination of metal alloys, pyrolite carbon and dacron. Types of Mechanical Valves Caged-ball Valve (Star-Edwards) A me

Cultural and religious values, Wildlife has influenced language, art, relig...

Wildlife has influenced language, art, religion and social customs of many societies worldwide, and wild animals figure prominently in many cultures even today. In all cultures of

What are the habits effect on implant, Habits: Any abnormal habits need...

Habits: Any abnormal habits needs to be identified. Following habits may have detrimental effect on the implant: 1. Parafunction -  Bruxism/ Clenching 2. Tongue Thrusting

What is cerebrum, What is Cerebrum Cerebrum is divided into right and l...

What is Cerebrum Cerebrum is divided into right and left parts known as cerebral hemisphere. It is the largest part of the brain. The functions of the cerebrum:  It recei

How is the human karyotype usually represented, Can two normal individuals ...

Can two normal individuals of the same species with sexual reproduction have identical genomes and identical karyotypes? How is the human karyotype usually represented? Except

What is Inoculation and Culturing in Microbiology, What is Inoculation and ...

What is Inoculation and Culturing? In microbiology, inoculation is the process where a microbe of interest is introduced into a previously sterilized growth medium for the p

Theory of embryology - gradient theory, GRADIEN T THEORY - It was give...

GRADIEN T THEORY - It was given by T. Boveri & was supported by M.C. Child. Child called it as metabolic axial gradient theory. According to it, the egg has distinct ani

Define food behaviour - public nutrition, Define Food Behaviour - Public Nu...

Define Food Behaviour - Public Nutrition? Food is not merely a means to survival but the fuel that drives the human body and the economic engine. What influences what we decide

Trisaccharides, TRISACCHARIDES The oligosaccharides are made of three m...

TRISACCHARIDES The oligosaccharides are made of three monosaccharide residues. A common trisaccharides is Raffinose which is formed by condensation of galactose-glucose-fr

Fluids requirement in chronic diarrhoea, Q. Fluids requirement in chronic d...

Q. Fluids requirement in chronic diarrhoea? Fluids: Intake should be liberal to minimize the risk of dehydration. Remember we read about fluid management in this section before

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd