Explain the gram staining technique, Biology

Assignment Help:

Explain the Gram Staining technique?

Gram staining is a differential staining procedure which divides bacteria into two groups: Gram positive and Gram negative, based on staining properties. The gram stain was developed in 1884 by the Danish Physician, Dr. Christian Gram. It is one of the most important and widely employed staining procedure in bacteriology.

It is very useful and is almost essential in identifying an unknown bacteria. The basis of gram staining is the difference in cell wall composition of two types of bacterial cells. Gram positives have thick cell wall, made up of multi-layered peptidoglycan with extensive cross-linking. On the other hand, gram negative cell wall has a very thin peptidoglycan layer surrounded by outer lipid rich outer membrane. There is no outer membrane in case of gram positives. This difference in cell wall leads to decolourization of gram negatives but not gram positives by decolourizing agent i.e., ethanol or acetone.


Related Discussions:- Explain the gram staining technique

Where in the body is the thyroid gland located, Q. Where in the body is the...

Q. Where in the body is the thyroid gland located? The thyroid is located in the anterior cervical region (frontal neck) in just below the larynx and front of the trachea and i

Explain vernalisation, Name the state in humans in which the blood choleste...

Name the state in humans in which the blood cholesterol content becomes abnormally high. a) Explain vernalisation. b) Many villagers near Industrial area vary from "blue bab

Ecological pyramids, What are the advantages of ecological pyramids?

What are the advantages of ecological pyramids?

Explain offspring of heterozygous for autosomal trait mates, If a male who ...

If a male who is heterozygous for an autosomal trait mates with a female who is also heterozygous for that trait, what percent of their offspring are probable to be heterozygous fo

Explain the summary of morbidity in cad, Explain the summary of morbidity ...

Explain the summary of morbidity  in CAD? i) The prevalence of CAD among immigrant Indians is about three-fold higher than in comparable indigenous populations; ii) CAD tends t

Photosynthesis, why we add some sodium bicarbonate in water?

why we add some sodium bicarbonate in water?

Nature of light - floral induction, Nature of Light - Floral Induction ...

Nature of Light - Floral Induction Red light promotes flowering in short day plants. If the dark regime of a short day plant is interrupted by red light, flowering is inhibite

Explain about the stilbenes, Explain about the Stilbenes? Stilbenes con...

Explain about the Stilbenes? Stilbenes contain 3 phenyl compounds connected by a 2-carbon methylene bridge and occur in nature in restricted distribution. In plants, they act a

Most abundant form under which nitrogen is found in nature, Q. What is the ...

Q. What is the most abundant form under which nitrogen is found in nature? The major abundant nitrogen-containing molecule found in nature is molecular nitrogen (N2). The air i

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd