Explain the gram staining technique, Biology

Assignment Help:

Explain the Gram Staining technique?

Gram staining is a differential staining procedure which divides bacteria into two groups: Gram positive and Gram negative, based on staining properties. The gram stain was developed in 1884 by the Danish Physician, Dr. Christian Gram. It is one of the most important and widely employed staining procedure in bacteriology.

It is very useful and is almost essential in identifying an unknown bacteria. The basis of gram staining is the difference in cell wall composition of two types of bacterial cells. Gram positives have thick cell wall, made up of multi-layered peptidoglycan with extensive cross-linking. On the other hand, gram negative cell wall has a very thin peptidoglycan layer surrounded by outer lipid rich outer membrane. There is no outer membrane in case of gram positives. This difference in cell wall leads to decolourization of gram negatives but not gram positives by decolourizing agent i.e., ethanol or acetone.


Related Discussions:- Explain the gram staining technique

Define regulation of water balance for the kidneys, Define Regulation of Wa...

Define Regulation of Water Balance For the kidneys? The kidneys are highly efficient conservators of body water. Although the kidneys filter 180 L of blood per day (125 ml / mi

Determine the axon of a nerve cell, In the axon of a nerve cell, A. the...

In the axon of a nerve cell, A. the voltage-dependent conductance of the voltage-gated potassium channel has a faster time course than the voltage-dependent conductance of the

Fertilization, Fertilization is the fusion of the two gametes (sperm and o...

Fertilization is the fusion of the two gametes (sperm and ovum) to produce a zygote which develops into the new individual with a genetic heritage derived from both the parents. S

Define altered glucose metabolism - nutrition during stress, Define Altered...

Define Altered Glucose Metabolism - Nutrition during Stress? Blood glucose levels are generally elevated in the infected patient but plasma insulin levels are normal or even hi

Explain advantages of periodontal ligament, Explain periodontal ligament ...

Explain periodontal ligament The absence of the periodontal ligament and the presence of osseointegration instead of the gomphosis joint leads to the difference in movement un

Main components of blood plasma, Main Components of Blood Plasma You w...

Main Components of Blood Plasma You would recall the various types of cells found in blood. The composition of human blood plasma is given in Table. The very structure of bloo

Atp, ATP The energy currency of the body One of the monomers u...

ATP The energy currency of the body One of the monomers used in the synthesis of RNA Contains three phosphate groups àhigh energy Removal of one releases e

Explain aortic valvuloplasty, Q. Explain Aortic Valvuloplasty? Valvar a...

Q. Explain Aortic Valvuloplasty? Valvar aortic stenosis accounts for 4-6 per cent of CHD. LV Outflow Tract obstruction eventually leads to LV dysfunction and congestive heart f

Describe ecological phytosociological, Q. Describe Ecological phytosociolog...

Q. Describe Ecological phytosociological? Field studies are desirable for an understanding of the relationship of any group of plants. If it is not possible to study the plants

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd