Explain the gram staining technique, Biology

Assignment Help:

Explain the Gram Staining technique?

Gram staining is a differential staining procedure which divides bacteria into two groups: Gram positive and Gram negative, based on staining properties. The gram stain was developed in 1884 by the Danish Physician, Dr. Christian Gram. It is one of the most important and widely employed staining procedure in bacteriology.

It is very useful and is almost essential in identifying an unknown bacteria. The basis of gram staining is the difference in cell wall composition of two types of bacterial cells. Gram positives have thick cell wall, made up of multi-layered peptidoglycan with extensive cross-linking. On the other hand, gram negative cell wall has a very thin peptidoglycan layer surrounded by outer lipid rich outer membrane. There is no outer membrane in case of gram positives. This difference in cell wall leads to decolourization of gram negatives but not gram positives by decolourizing agent i.e., ethanol or acetone.


Related Discussions:- Explain the gram staining technique

Biological sickness of soil, what is biological sickness of soil and its ma...

what is biological sickness of soil and its management

Peak flow velocity, Peak Flow Velocity  fs  derived  from Doppler  shift by...

Peak Flow Velocity  fs  derived  from Doppler  shift by  rearranging Doppler equation: V = C/2 x ΔF/Fo Various  information can be obtained from spectral display

Before purchasing - nursing software, Before Purchasing  Ideally it...

Before Purchasing  Ideally it's wise to buy a readymade software with options to further customize it to suit your managerial functions.   A totally Custom-made softwar

In vivo imaging in psychiatry, In vivo imaging in psychiatry To illustr...

In vivo imaging in psychiatry To illustrate the ingenious applications to which in vivo imaging can be put, consider the use of PET in the study of hallucinations by Frith and

Define introduction of lipids, Define Introduction of Lipids? You have ...

Define Introduction of Lipids? You have studied that lipids together with carbohydrates and proteins are important as food for many animals. Lipids include neutral fats, phosph

Main moral problem about the cloning of human individuals, What is the main...

What is the main moral problem about the cloning of human individuals? As well biological perils, a very serious moral problem involves the nucleus transplantation technology c

Supportive evidence - erythema marginatum, Minor Criteria These are arth...

Minor Criteria These are arthralgia, fever, prolonged PR interval, raised ESR and C-reactive protein levels. In some cases abdominal pain and epistaxis may occur. Other non spec

Reactants of photosynthesis, what are the reactants and the products of pho...

what are the reactants and the products of photosynthesis? under the thylakoid, stroma,and combined

Human physiology, Carbohydrates and proteins question #Minimum 100 words a...

Carbohydrates and proteins question #Minimum 100 words accepted#

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd