Explain the gram staining technique, Biology

Assignment Help:

Explain the Gram Staining technique?

Gram staining is a differential staining procedure which divides bacteria into two groups: Gram positive and Gram negative, based on staining properties. The gram stain was developed in 1884 by the Danish Physician, Dr. Christian Gram. It is one of the most important and widely employed staining procedure in bacteriology.

It is very useful and is almost essential in identifying an unknown bacteria. The basis of gram staining is the difference in cell wall composition of two types of bacterial cells. Gram positives have thick cell wall, made up of multi-layered peptidoglycan with extensive cross-linking. On the other hand, gram negative cell wall has a very thin peptidoglycan layer surrounded by outer lipid rich outer membrane. There is no outer membrane in case of gram positives. This difference in cell wall leads to decolourization of gram negatives but not gram positives by decolourizing agent i.e., ethanol or acetone.


Related Discussions:- Explain the gram staining technique

What is the disease caused by vitamin d deficiency, What is the disease cau...

What is the disease caused by vitamin D deficiency? Which tissue does it affect? The lack of vitamin D causes the disease called as rickets (rachitis), characterized by decalci

Protein synthesis, Protein Synthesis The central dogma of modern bioche...

Protein Synthesis The central dogma of modern biochemistry is totally based on the coded information holds within deoxyribonucleic acid (DNA). Double stranded DNA is converted

Are the arterial pulsations normal, Are the arterial pulsations normal? ...

Are the arterial pulsations normal? Is the pulse volume normal or increased? Is there a discrepancy of pulsation in any of the four extremities? A careful evaluation of pulsati

Preference share capital or quasi-equity, Preference Share Capital or Quasi...

Preference Share Capital or Quasi-Equity It is also called quasi-equity because it combines features of equity and those of debt.  It is preference because it is preferred to

What is the digestive enzyme that acts within the stomach, What is the dige...

What is the digestive enzyme that acts within the stomach? Which type of food does it digest? What are the cells that produce that enzyme? The digestive enzyme that acts in the

Modification of mitosis, MODIFICATION OF MITOSIS 1 .      CRYPTOMITOS...

MODIFICATION OF MITOSIS 1 .      CRYPTOMITOSIS OR PROMITOSIS : Primitive type of mitosis. In this mitosis nuclear membrane not disappear (remain intact throughout the div

Describe the recovery process of an extracellular product, Question 1: ...

Question 1: Describe the recovery process of an extracellular product through downstream processing. Different criteria's for the choice of recovery process Brief on

Plant physiology, why does the removal of extremity of coleoptile prohibit ...

why does the removal of extremity of coleoptile prohibit plant growth?

What is the difference between the concepts of genome, Q. What is the diffe...

Q. What is the difference between the concepts of genome and karyotype? Genome is the set of DNA molecules that characterizes each species or each living being. The concept the

ADAPTATION, AQUATIC ADAPTATION IN ANIMALS

AQUATIC ADAPTATION IN ANIMALS

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd