Explain the gram staining technique, Biology

Assignment Help:

Explain the Gram Staining technique?

Gram staining is a differential staining procedure which divides bacteria into two groups: Gram positive and Gram negative, based on staining properties. The gram stain was developed in 1884 by the Danish Physician, Dr. Christian Gram. It is one of the most important and widely employed staining procedure in bacteriology.

It is very useful and is almost essential in identifying an unknown bacteria. The basis of gram staining is the difference in cell wall composition of two types of bacterial cells. Gram positives have thick cell wall, made up of multi-layered peptidoglycan with extensive cross-linking. On the other hand, gram negative cell wall has a very thin peptidoglycan layer surrounded by outer lipid rich outer membrane. There is no outer membrane in case of gram positives. This difference in cell wall leads to decolourization of gram negatives but not gram positives by decolourizing agent i.e., ethanol or acetone.


Related Discussions:- Explain the gram staining technique

Nutrition, define alimentary canal

define alimentary canal

How can the immune memory lead to the efficacy of vaccines, How can the imm...

How can the immune memory lead to the efficacy of vaccines and also produce allergies? Vaccines are controlled inoculations of fragments of infectious agents or of inactive inf

Culture-negative endocarditis, Special studies to diagnose IE caused by fas...

Special studies to diagnose IE caused by fastidious bacteria and other organisms must be performed (serological studies). Thereafter, unless clinical or epidemiologic clues suggest

What happen when auxin concentration in plant is over, What happens when au...

What happens when auxin concentration in some structures of the plant is over the action range of the hormone? In some of parts of the plant (roots, stem, lateral buds) there a

Cell cycle, The cell cycle undergoes a sequence of changes which invol...

The cell cycle undergoes a sequence of changes which involve a period of growth replication of DNA, Followed by cell division. This sequence of changes is called cell cycle.

Assume the effect on turnover, Explain two ways in which a changing climate...

Explain two ways in which a changing climate may impact the nutrient cycle. Predict  the effect on turnover.

Explain the differance between savanna and desert, Explain the differance b...

Explain the differance between savanna and desert? Savanna : Savanna is characterized by relatively low rainfall and pronounced dry seasons. This type of climate produces a b

Explain the deficiency and toxicity of vitamin a, Explain the Deficiency an...

Explain the Deficiency and Toxicity of vitamin A? Who defines VAD as tissue concentrations of vitamin A low enough to have adverse health consequences even if there is no evide

How antigen react against future infection by same agent, Q. How can an org...

Q. How can an organism that once underwent contact with an antigen be immunized against future infections by the same agent? This phenomenon is called as immune memory when an

Describe the physiology of atrial septal defect, Describe the Physiology of...

Describe the Physiology of atrial septal defect? In ASD the magnitude of left to right shunt is determined by the size of the defect and the relative compliance of the right ve

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd