Explain the gram staining technique, Biology

Assignment Help:

Explain the Gram Staining technique?

Gram staining is a differential staining procedure which divides bacteria into two groups: Gram positive and Gram negative, based on staining properties. The gram stain was developed in 1884 by the Danish Physician, Dr. Christian Gram. It is one of the most important and widely employed staining procedure in bacteriology.

It is very useful and is almost essential in identifying an unknown bacteria. The basis of gram staining is the difference in cell wall composition of two types of bacterial cells. Gram positives have thick cell wall, made up of multi-layered peptidoglycan with extensive cross-linking. On the other hand, gram negative cell wall has a very thin peptidoglycan layer surrounded by outer lipid rich outer membrane. There is no outer membrane in case of gram positives. This difference in cell wall leads to decolourization of gram negatives but not gram positives by decolourizing agent i.e., ethanol or acetone.


Related Discussions:- Explain the gram staining technique

Explain about the glycoproteins, Explain about the Glycoproteins? Most...

Explain about the Glycoproteins? Most of the naturally occurring conjugated proteins are glycoproteins. Sugar molecules are covalently bound to them, especially those secreted

Natality rate - natality, Natality Rate - Natality Natality rate or bi...

Natality Rate - Natality Natality rate or birth rate is determined by dividing the number of individuals born by unit time and is expressed as follows: Natality rate =  ΔNn

Explain about dry heat sterilization, Q. Explain about Dry heat sterilizati...

Q. Explain about Dry heat sterilization? It is used to sterilize glass ware, forceps, scissors, scalpels, all glass syringes, swabs, some pharmaceuticals products such as liqui

Myocardial hypertrophy and remodeling, The long term adaptive mechanisms in...

The long term adaptive mechanisms involve myocardial hypertrophy and remodeling which occurs slowly over weeks to months. The capacity of these adaptive mechanisms meant to sust

How many chromosomes and dna molecules found per cell, A cell in G1 of inte...

A cell in G1 of interphase has 12 chromosomes. How many chromosomes and DNA molecules will be found per cell when this original cell progresses to the G2?

A decrease in blood plasma levels of calcium, A decrease in blood plasma le...

A decrease in blood plasma levels of calcium will lead to A. an increase in the calcium ion excretion in the urine. B. an increase in the calcium ion absorption from the con

Hybridization, what is hybridization,and what are its different types??

what is hybridization,and what are its different types??

How is gas exchange done in flatworms, Q. How is gas exchange done in flatw...

Q. How is gas exchange done in flatworms? Platyhelminthes exchange gases exclusively by diffusion through their body surface. This is only possible because all cells are locali

Explain the microscope, Explain the Microscope? Microscope is a powerfu...

Explain the Microscope? Microscope is a powerful and crucial basic tool in the field of microbiology. Microbiology, as we have already studied in our theory Course, is the scie

Sarcolemma, Normal 0 false false false EN-IN X-NONE ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd