Explain the gram staining technique, Biology

Assignment Help:

Explain the Gram Staining technique?

Gram staining is a differential staining procedure which divides bacteria into two groups: Gram positive and Gram negative, based on staining properties. The gram stain was developed in 1884 by the Danish Physician, Dr. Christian Gram. It is one of the most important and widely employed staining procedure in bacteriology.

It is very useful and is almost essential in identifying an unknown bacteria. The basis of gram staining is the difference in cell wall composition of two types of bacterial cells. Gram positives have thick cell wall, made up of multi-layered peptidoglycan with extensive cross-linking. On the other hand, gram negative cell wall has a very thin peptidoglycan layer surrounded by outer lipid rich outer membrane. There is no outer membrane in case of gram positives. This difference in cell wall leads to decolourization of gram negatives but not gram positives by decolourizing agent i.e., ethanol or acetone.


Related Discussions:- Explain the gram staining technique

Explain the feeding 6-12 months infant, Explain the Feeding 6-12 Months Inf...

Explain the Feeding 6-12 Months Infant? As the infant grows older, mother's milk alone is not sufficient to meet his increasing needs. This could be seen in few infants between

What proportion of the progeny, In holly, serrated leaves are dominant to s...

In holly, serrated leaves are dominant to smooth-edged leaves, and red berries are dominant to green berries. Two holly plants heterozygous for leaf edge shape and berry color are

Qualitative changes, Qualitative Changes The qualitative changes in th...

Qualitative Changes The qualitative changes in the structure of proteins in response to stress can lead to the following: Resistance against denaturation of prote

Explain the four important basic points dietician, To develop the food exch...

To develop the food exchange list, a doctor or dietician keeps in mind four important basic points. These are: 1) Group similar foods in one group. For example cereals, roots,

Electron transport chain, ELECTRON TRANSPORT CHAIN All processes requir...

ELECTRON TRANSPORT CHAIN All processes require energy. In living cells, we constantly use energy for a number of biochemical reactions e.g. muscular movements, synthesis  of ne

Explain nelfinavir, Explain Nelfinavir (NFV, Viracept)  Nelfinavir is a...

Explain Nelfinavir (NFV, Viracept)  Nelfinavir is a commonly used protease inhibitor because it is well tolerated. It appears to be less potent than lopinavir/ritonavir or efav

Animal nutrition, What are fluid and deposit feeding in holozoic animals

What are fluid and deposit feeding in holozoic animals

Can you explain radula, Q What is the radula? What is the function of this ...

Q What is the radula? What is the function of this organ? Some molluscs have a tongue-like structure with harsh saliences similar to small teeth. This structure is called as ra

Mass dispersal - population dispersal, Mass Dispersal - Population Dispersa...

Mass Dispersal - Population Dispersal A population is inherently dynamic in nature since individuals are always leaving or entering the populations. But such changes normally

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd