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Explain the Gram Staining technique?
Gram staining is a differential staining procedure which divides bacteria into two groups: Gram positive and Gram negative, based on staining properties. The gram stain was developed in 1884 by the Danish Physician, Dr. Christian Gram. It is one of the most important and widely employed staining procedure in bacteriology.
It is very useful and is almost essential in identifying an unknown bacteria. The basis of gram staining is the difference in cell wall composition of two types of bacterial cells. Gram positives have thick cell wall, made up of multi-layered peptidoglycan with extensive cross-linking. On the other hand, gram negative cell wall has a very thin peptidoglycan layer surrounded by outer lipid rich outer membrane. There is no outer membrane in case of gram positives. This difference in cell wall leads to decolourization of gram negatives but not gram positives by decolourizing agent i.e., ethanol or acetone.
What is the menstrual cycle? The menstrual cycle is the periodic succession of interactions among hormones and the organs of the female reproductive system that, after the star
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Q. What are the ions? What are the two kinds of molecules into which ions are classified ? Ions are substances or atoms electrically charged by means of gain or loss of electron
how does a cell formed
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Table gives the current classification of hypertension as recommended by JNC VII. The classification is based on the mean of two or more seated readings on two or more occasions.
Explain the Sub-Culturing? Microbiological studies require microorganisms in pure form, i.e., a single type of microorganisms should be present in a culture. Maintenance of the
In developing countries because of poor compliance, poverty, ignorance, primary and secondary prevention strategies are difficult to implement properly. Also in western countries (
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