Explain the gram staining technique, Biology

Assignment Help:

Explain the Gram Staining technique?

Gram staining is a differential staining procedure which divides bacteria into two groups: Gram positive and Gram negative, based on staining properties. The gram stain was developed in 1884 by the Danish Physician, Dr. Christian Gram. It is one of the most important and widely employed staining procedure in bacteriology.

It is very useful and is almost essential in identifying an unknown bacteria. The basis of gram staining is the difference in cell wall composition of two types of bacterial cells. Gram positives have thick cell wall, made up of multi-layered peptidoglycan with extensive cross-linking. On the other hand, gram negative cell wall has a very thin peptidoglycan layer surrounded by outer lipid rich outer membrane. There is no outer membrane in case of gram positives. This difference in cell wall leads to decolourization of gram negatives but not gram positives by decolourizing agent i.e., ethanol or acetone.


Related Discussions:- Explain the gram staining technique

What is the formation of atherosclerosis plaques, Which of the following le...

Which of the following leads to the formation of atherosclerosis plaques? -uptake of oxidized LDL particles by macrophase -non specific interactions between LDL particles

What is dyslipidaemia, What is Dyslipidaemia ? The results of epidemiol...

What is Dyslipidaemia ? The results of epidemiological studies, as well as trials with angiographic or clinical endpoints, confirm the importance of various lipid fractions in

Normal color vision- color blindness, If a color blind man marries a woman ...

If a color blind man marries a woman pure for normal color vision, it's probable that one of the below situations may result. Is it possible that a) All the children would be

Explain the formation of enzyme, Explain the Formation of enzyme? Form...

Explain the Formation of enzyme? Formation of enzyme: Vitamin D is essential for the formation of two enzymes -  alkaline phosphtase in the intestinal lining (involved in cal

Describe directly and indirectly modes of transcription , 1.  A completely ...

1.  A completely new protein has been synthesized by a research lab. You are inadvertently exposed to it and have an allergic response. What type of antibody initiates this respons

Define body mass index (bmi) or quetelet index, Define Body Mass Index (BMI...

Define Body Mass Index (BMI) or Quetelet Index? BMI is an index measure of body fat used for the assessment of disease risk.  It is a figure which gives an indication of weight

Assessment and nursing care plans, Assessment and Nursing Care Plans C...

Assessment and Nursing Care Plans Clustered Case Study Mr. Smith is an 83 year old gentleman (DOB 1.2.1928), who lives alone in his home on a cattle farm west of Ravenshoe.

Deficiency diseases-neonatal hypoglycaemia, Neonatal  hypoglycaemia Hy...

Neonatal  hypoglycaemia Hypoglycaemia is a metabolic condition of newborn piglets that develops in first few days of life due to decreased caloric intake and increased cataboli

Explain the occurrence of vitamin C, Explain the Occurrence of Vitamin C ...

Explain the Occurrence of Vitamin C Vitamin C (ascorbic acid) is an active ingredient present in any animal or vegetable cell which occurs in the plant in free form and also bo

Diagnosis of meningitis, Diagnosis Lumbar puncture is done which shows...

Diagnosis Lumbar puncture is done which shows cloudy CSF with increased pressure, the white cell count predominantly contains neutrophils. CSF proteins are increased but sug

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd