Explain the gram staining technique, Biology

Assignment Help:

Explain the Gram Staining technique?

Gram staining is a differential staining procedure which divides bacteria into two groups: Gram positive and Gram negative, based on staining properties. The gram stain was developed in 1884 by the Danish Physician, Dr. Christian Gram. It is one of the most important and widely employed staining procedure in bacteriology.

It is very useful and is almost essential in identifying an unknown bacteria. The basis of gram staining is the difference in cell wall composition of two types of bacterial cells. Gram positives have thick cell wall, made up of multi-layered peptidoglycan with extensive cross-linking. On the other hand, gram negative cell wall has a very thin peptidoglycan layer surrounded by outer lipid rich outer membrane. There is no outer membrane in case of gram positives. This difference in cell wall leads to decolourization of gram negatives but not gram positives by decolourizing agent i.e., ethanol or acetone.


Related Discussions:- Explain the gram staining technique

Pasteurellosis, Pasteurellosis Haemorrhagic septicaemia (HS) Synonym  ...

Pasteurellosis Haemorrhagic septicaemia (HS) Synonym    :    Gal Ghotu Causative organism B:2                              :    Pasteurella multocida Serotype Haemorrhage sept

Describe amoebae - paramecia and trichomonas, Q How do amoebae, paramecia a...

Q How do amoebae, paramecia and trichomonas respectively move? Amoebae move by amoeboid movements small invaginations and projections of their plasma membrane (pseudopods) that

Give choice of catheters for ventriculography, Q. Give Choice of Catheters ...

Q. Give Choice of Catheters for Ventriculography? 1) Pigtail Catheter: This catheter developed by Judkins has end hole and side holes. The end hole permits insertion of the c

Summary of determinants of health status, Normal 0 false fals...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

What is physiology root pressure explain briiefly, What is Physiology: Root...

What is Physiology: Root Pressure explain briiefly? Transport of water, minerals and nutrients within vascular plants is dramatically different from animals such as humans. Whe

To show the respiration of a plant, To show the respiration of a plant ...

To show the respiration of a plant Place the plant in a test tube held in a weighted wooden block. Put this in a bowl having lime water and cover the plant with a jar. Keep the

Compare the modes of intercellular communication, Compare the modes of inte...

Compare the modes of intercellular communication used by the endocrine and nervous systems and discuss the functional significance of the differences between the two systems.

List a few applications of starches in the food industry, List a few applic...

List a few applications of starches in the food industry. A few applications of starches in the foods industry include thickener, a fat sparing agent, adhesive, binder,

Polypeptide chains of hemoglobin, Q. In sickle cell anemia, a hereditary di...

Q. In sickle cell anemia, a hereditary disease, there is replacement of one amino acid by another in one of the four polypeptide chains of hemoglobin. In this case are all of the s

Determine the most probable mode of inheritance, In the following pedigrees...

In the following pedigrees, the disorders or traits presented follow simple patterns of Mendelian inheritance. For each trait, determine the most probable mode of inheritance, stat

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd