Explain the gram staining technique, Biology

Assignment Help:

Explain the Gram Staining technique?

Gram staining is a differential staining procedure which divides bacteria into two groups: Gram positive and Gram negative, based on staining properties. The gram stain was developed in 1884 by the Danish Physician, Dr. Christian Gram. It is one of the most important and widely employed staining procedure in bacteriology.

It is very useful and is almost essential in identifying an unknown bacteria. The basis of gram staining is the difference in cell wall composition of two types of bacterial cells. Gram positives have thick cell wall, made up of multi-layered peptidoglycan with extensive cross-linking. On the other hand, gram negative cell wall has a very thin peptidoglycan layer surrounded by outer lipid rich outer membrane. There is no outer membrane in case of gram positives. This difference in cell wall leads to decolourization of gram negatives but not gram positives by decolourizing agent i.e., ethanol or acetone.


Related Discussions:- Explain the gram staining technique

Is herbivorism a form of predatism, Q. Is herbivorism a form of predatism? ...

Q. Is herbivorism a form of predatism? The Herbivorism is a form of predatism in which first order consumers feed from producers (plants or algae). For instance, fruits and bir

Why overwatering a potted tomato plant will kill it, Consistently overwater...

Consistently overwatering a potted tomato pla nt will eventually kill it. Using the map, suggest why waterlogged soil results in plant death. O2 cannot reach respiring root cells.

Define natural disasters - public nutrition, Define Natural Disasters - Pub...

Define Natural Disasters - Public Nutrition? Disaster prone settings also shape the intensity and prevalence of food insecurity, these consist of poor who are exposed to recurr

How does glucose help in the formation of atp from adp?, GLUCOSE IS OXIDISE...

GLUCOSE IS OXIDISED TO PRODUCE ENERGY TO GIVE ADP TO FORM ATP

Express the term - amnion, Express the term - Amnion One of the extraem...

Express the term - Amnion One of the extraembryonic membranes found in terrestrial vertebrate. The amnion is filled with fluid and the developing embryo of reptiles, birds, and

Explain process of determination of vitamin B6, Explain process of Determin...

Explain process of Determination of vitamin B 6 Colorimetric determination of indophenol dye using 2,6-dichloroquinone chloraimide as colour reagent or diethyl-p-phenylenediam

Define micronutrients requirements for anorexia nervosa, Define Micronutrie...

Define Micronutrients Requirements for Anorexia Nervosa? It is advisable to include one 100% RDA multivitamin tablet with minerals. The use of prophylactic thiamine supplements

Genetic abnormalities caused by recessive genes, What are some diseases or ...

What are some diseases or genetic abnormalities caused by recessive genes? Instance of recessive genetic diseases are: cystic fibrosis, phenylketonuria, albinism, galactosemia,

Define abbe condenser of microscope, Define Abbe Condenser of Microscope? ...

Define Abbe Condenser of Microscope? It is present beneath the stage, as shown in Figure. It collects and focuses a cone of light on the slide. Its position can be adjusted ver

What is burden of rheumatic heart diseases, What is Burden of Rheumatic Hea...

What is Burden of Rheumatic Heart Diseases? Although in the twenty-first century RHD has been eradicated in western countries, in India and other developing countries it contin

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd