Explain the equilibrium dialysis, Biology

Assignment Help:

Explain the Equilibrium Dialysis?

Equilibrium dialysis is a useful technique for studying the binding of a small uncharged solute species (a ligand) to a macromolecule. The macromolecule solution is placed on one side of a membrane through which it cannot pass, with a solution without the macro- molecule on the other side, and the ligand is allowed to come to transfer equilibrium across the membrane. If the same solute standard state is used for the ligand in both solutions, at equilibrium the unbound ligand must have the same activity in both solutions. Measure- ments of the total ligand molality in the macromolecule solution and the ligand molality in the other solution, combined with estimated values of the unbound ligand activity coefficients, allow the amount of ligand bound per macromolecule to be calculated.


Related Discussions:- Explain the equilibrium dialysis

Organic molecules, Organic Molecules Some organic molecules in life...

Organic Molecules Some organic molecules in life are Carbohydrates and Lipids à C, H and O Proteins à C, H, O, N & sometimes S Nucleic acids à C, H, O, N & P

Explain retrograde peri-implantitis, What is retrograde peri-implantitis? ...

What is retrograde peri-implantitis? Retrograde peri-implantitis has been described by Misch as implant failure probably due to bone microfractures caused by premature implant

Determine the separation of homologous chromosomes, During mitotic anaphase...

During mitotic anaphase is there separation of homologous chromosomes or separation of identical chromatids? In the anaphase of mitosis the identical chromatids separate and co

Equine herpes virus infections , Equine herpes virus infections (equine rhi...

Equine herpes virus infections (equine rhinopneumonitis, abortions) Equine herpes virus infections in horses and foal are caused by Alphaherpes virus of herpesviridae family p

Airway management - respiratory failure, Airway Management To improve...

Airway Management To improved ventilation, suctioning, IPPB, Ultrasonic mist therapy and postural drainage with clapping and vibrating are all employed to halt the progress o

What are the characteristics of thick gingiva, Characteristics of Thick Gin...

Characteristics of Thick Gingiva  Relatively flat soft tissue and bony architecture  Dense fibrotic soft tissue  Relatively large amount of attached gingiva  Thick und

Explain the nucleic acid is the transforming agent or not, Fredrick Griffit...

Fredrick Griffith published a series of experiments that collectively were dubbed "The Transforming Principle" in 1928. Which of the following prevented him from being able to decl

State the term - halsted reitan varies, State the term - Halsted Reitan var...

State the term - Halsted Reitan varies with the particular test Scoring for the Halsted Reitan varies with the particular test, such that individual scores may be expressed in

What is the action mechanism of the antiretroviral drugs, Q. What is the ac...

Q. What is the action mechanism of the antiretroviral drugs known protease inhibitors which are used against HIV infection? Protease inhibitors are some of the antiretroviral d

Tetrasaccharides, TETRASACCHARIDES The oligosaccharides are formed b...

TETRASACCHARIDES The oligosaccharides are formed by condensation of four monosaccharides. T rachyos e is a nonreducing tetrasaccharide found in plants as transport ca

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd