Explain the equilibrium dialysis, Biology

Assignment Help:

Explain the Equilibrium Dialysis?

Equilibrium dialysis is a useful technique for studying the binding of a small uncharged solute species (a ligand) to a macromolecule. The macromolecule solution is placed on one side of a membrane through which it cannot pass, with a solution without the macro- molecule on the other side, and the ligand is allowed to come to transfer equilibrium across the membrane. If the same solute standard state is used for the ligand in both solutions, at equilibrium the unbound ligand must have the same activity in both solutions. Measure- ments of the total ligand molality in the macromolecule solution and the ligand molality in the other solution, combined with estimated values of the unbound ligand activity coefficients, allow the amount of ligand bound per macromolecule to be calculated.


Related Discussions:- Explain the equilibrium dialysis

Cell, Ask question #Minimum cell theory 100 words accepted#

Ask question #Minimum cell theory 100 words accepted#

Explain nsp and food hydrocolloids, NSP NSP or dietary fibre is the nam...

NSP NSP or dietary fibre is the name given to a group of materials found in the cell walls of plants which gives the plant its structure and form. Food hydrocolloids Fo

Induction of defensive barriers in plants, Induction of Defensive Barriers ...

Induction of Defensive Barriers in Plants Several polymers deposited near surface of plants form an effective barrier against invasion or stress. Cuticle (which consists of a

What are catalysts, Normal 0 false false false EN-IN ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Water: An Essential for Life, What is the reason behind the high surface te...

What is the reason behind the high surface tension of water?

Genes and alleles, Genes and Alleles The inheritance of any character c...

Genes and Alleles The inheritance of any character can be studied only when thcre are two contrasting conditions, such as yellow versus green seed colour (as observed by Mendel

Ovipary, Ovipary, Vivipary and Ovovivipary In each case of external a...

Ovipary, Vivipary and Ovovivipary In each case of external and in some cases of internal fertilisation, development of the fertilized egg (zygote) occurs outside the mother's

Explain the aseptic technique - sub culturing, Explain the Aseptic Techniqu...

Explain the Aseptic Technique - Sub Culturing? Aseptic technique is a series of procedures used to prevent contamination of cultures and sterile culture media. It is one of the

Factors that affects oxygen dissociation curves, Factors that affects Oxyge...

Factors that affects Oxygen Dissociation Curves Oxygen dissociation curve for a sample of blood is affected by several factors. The most important of them are: Te

What would be its final volume, Consider a simple spherical model cell that...

Consider a simple spherical model cell that consists of cytoplasm and a plasma membrane. The cell's initial volume is 2 nL and contains 0.2 M protein. The cell is placed in a la

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd