Explain the equilibrium dialysis, Biology

Assignment Help:

Explain the Equilibrium Dialysis?

Equilibrium dialysis is a useful technique for studying the binding of a small uncharged solute species (a ligand) to a macromolecule. The macromolecule solution is placed on one side of a membrane through which it cannot pass, with a solution without the macro- molecule on the other side, and the ligand is allowed to come to transfer equilibrium across the membrane. If the same solute standard state is used for the ligand in both solutions, at equilibrium the unbound ligand must have the same activity in both solutions. Measure- ments of the total ligand molality in the macromolecule solution and the ligand molality in the other solution, combined with estimated values of the unbound ligand activity coefficients, allow the amount of ligand bound per macromolecule to be calculated.


Related Discussions:- Explain the equilibrium dialysis

Ascariasis, Ascariasis This disease is found in young calves causing di...

Ascariasis This disease is found in young calves causing digestive disturbances and poor growth. E t iology: It is caused by Toxocara vitulorum in cow and buffal

Financing of healthcare - constraints, Financing of Healthcare - Constraint...

Financing of Healthcare - Constraints One of the major constraints to improve the healthcare delivery system in developing countries is weak public sector management. These ec

Endosperm of gymnosperms and the endosperm of angiosperms, How dissimilar a...

How dissimilar are the endosperm of gymnosperms and the endosperm of angiosperms? In the gymnosperms the endosperm is haploid (n), it is called as primary endosperm. In the ang

Products that can be recycled, It requires the existence of a reliable mark...

It requires the existence of a reliable market for the recovered materials. Although the recovery of certain materials such as aluminium cans large plastic bottles can be profitabl

What is the vascular cambium, What is the vascular cambium? What is its fun...

What is the vascular cambium? What is its function? Vascular cambium is the secondary meristematic tissue that in roots and in the stem creates the vascular tissues (xylem and

Do plants have tissue organization and specialized organs, Do plants have t...

Do plants have tissue organization and specialized organs? Plants have specialized organs (as reproductive organs, roots, limbs, leaves) and differentiated tissues (vascular t

From which germ layer epidermis and nervous system originate, Q. From which...

Q. From which germ layer do the epidermis and the nervous system originate? What are other organs and tissues made from that germ layer? Epidermis and nervous system have the s

Cells, describe the cell theory and its exception

describe the cell theory and its exception

Sketch structure of ethyl ammonium, Draw structure of ethyl ammonium with a...

Draw structure of ethyl ammonium with all carbons and hydrogens.

Signify the term - trachea, Signify the term - Trachea Part of the resp...

Signify the term - Trachea Part of the respiratory system in insects, vertebrates and some spiders. In invertebrates it comprise tubules carrying air directly from outside to t

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd