Explain the equilibrium dialysis, Biology

Assignment Help:

Explain the Equilibrium Dialysis?

Equilibrium dialysis is a useful technique for studying the binding of a small uncharged solute species (a ligand) to a macromolecule. The macromolecule solution is placed on one side of a membrane through which it cannot pass, with a solution without the macro- molecule on the other side, and the ligand is allowed to come to transfer equilibrium across the membrane. If the same solute standard state is used for the ligand in both solutions, at equilibrium the unbound ligand must have the same activity in both solutions. Measure- ments of the total ligand molality in the macromolecule solution and the ligand molality in the other solution, combined with estimated values of the unbound ligand activity coefficients, allow the amount of ligand bound per macromolecule to be calculated.


Related Discussions:- Explain the equilibrium dialysis

Explain about the microcolony deft, Explain about the Microcolony DEFT? ...

Explain about the Microcolony DEFT? It allows determination of viable cells. Food homogenate is filtered through a DEFT membrane and then placed on the surface of appropriate c

Inrinsic conduction system of the heart, The cardiovascular system controls...

The cardiovascular system controls the movement of blood through thousands of miles of capillaries so that every tissue  in every part of the body is perfused. Essential nutrients

What is logic of the transfusional compatibility abo blood, What is the lo...

What is the logic of the transfusional compatibility concerning the ABO blood group system? A transfusional compatibility for the ABO system takes into account the antigens pre

Why plant life cycle known as alternation of generation, Why is the plant l...

Why is the plant life cycle known as alternation of generations? The plant life cycle is called as alternation of generations because in this cycle there are two dissimilar for

Vesicular stomatitis, Vesicular stomatitis Vesicular sto matitis virus ...

Vesicular stomatitis Vesicular sto matitis virus be longs to the ge nus Vesiculovirus in fa mily Rhabdoviridae. The disease clinically resembles foot-and-mouth disease, but is

Aves, detail information about ave?

detail information about ave?

What is the role of vitamin k in the blood coagulation, Q. What is the orga...

Q. What is the organ where most of the clotting factors are produced? What is the role of vitamin K in the blood coagulation? Most of the clotting factors are produced in the l

Define regulation of water balance - for digestive process, Define Regulati...

Define Regulation of Water Balance - For the Digestive Process? For the digestive process alone, the estimated daily volume of fluid that enters and leaves the gastrointestinal

Metabolism of pollen tubes, Metabolism of Pollen Tubes Pollen grains ...

Metabolism of Pollen Tubes Pollen grains contain auxins, and gibberellins which are known to be involved in post- pollination enlargement of the ovary and the development of

Concerning the number of hosts how are parasites classified, Q. Concerning ...

Q. Concerning the number of hosts how are parasites classified? The Parasites that require only one host are called as monoxenous parasites. The Parasites that need more than o

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd