Explain the equilibrium dialysis, Biology

Assignment Help:

Explain the Equilibrium Dialysis?

Equilibrium dialysis is a useful technique for studying the binding of a small uncharged solute species (a ligand) to a macromolecule. The macromolecule solution is placed on one side of a membrane through which it cannot pass, with a solution without the macro- molecule on the other side, and the ligand is allowed to come to transfer equilibrium across the membrane. If the same solute standard state is used for the ligand in both solutions, at equilibrium the unbound ligand must have the same activity in both solutions. Measure- ments of the total ligand molality in the macromolecule solution and the ligand molality in the other solution, combined with estimated values of the unbound ligand activity coefficients, allow the amount of ligand bound per macromolecule to be calculated.


Related Discussions:- Explain the equilibrium dialysis

Common symptoms linked to lactose intolerance, Q. Common symptoms linked to...

Q. Common symptoms linked to lactose intolerance? Common symptoms linked to lactose intolerance include: 1. Anorexia and nausea. 2. Intestinal distension 3. Abdominal

Photosynthesis, in a school laboratory, what is usually regarded as evidenc...

in a school laboratory, what is usually regarded as evidence that photosynthesis has occurred in plants

Bacteria and archaea been placed in a seperate domain, Why have bacteria an...

Why have bacteria and archaea been placed in a seperate domain?

Phylum protozoa, classification of organisms in phylum protozoa according t...

classification of organisms in phylum protozoa according to their class,order,family,genus,species.

Surgical treatment of mitral stenosis, Q. Surgical Treatment of mitral sten...

Q. Surgical Treatment of mitral stenosis? Available surgical treatment modalities include closed mitral valvotomy, open mitral valvotomy, and mitral valve replacement. With th

Impulse in the sensory neurone, Explain briefly how the stimulus at the fin...

Explain briefly how the stimulus at the finger produces an impulse in the sensory neurone using the following key words. 1. Energy conversion (linked to receptor) 2. Na+ in 3. Depo

What are the general necessities for surgery, General necessities for surge...

General necessities for surgery The actual surgical technique of dental implant placement, it would be appropriate to recapitulate the basic principles of surgery. The two prin

Percussion - criteria for endosteal implants, Percussion - criteria for end...

Percussion - criteria for endosteal implants? It is neither an indicator of clinical health nor of rigid fixation. Ringing sound that occurs on percussion corresponds to the pr

What is physiologically corrected transposition of arteries, What is Physio...

What is Physiologically corrected transposition of arteries? Physiologically corrected Transposition of the great arteries (ZTGA): Atrio- ventricular connections are also inapp

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd