Explain the equilibrium dialysis, Biology

Assignment Help:

Explain the Equilibrium Dialysis?

Equilibrium dialysis is a useful technique for studying the binding of a small uncharged solute species (a ligand) to a macromolecule. The macromolecule solution is placed on one side of a membrane through which it cannot pass, with a solution without the macro- molecule on the other side, and the ligand is allowed to come to transfer equilibrium across the membrane. If the same solute standard state is used for the ligand in both solutions, at equilibrium the unbound ligand must have the same activity in both solutions. Measure- ments of the total ligand molality in the macromolecule solution and the ligand molality in the other solution, combined with estimated values of the unbound ligand activity coefficients, allow the amount of ligand bound per macromolecule to be calculated.


Related Discussions:- Explain the equilibrium dialysis

Cell cycle, The cell cycle undergoes a sequence of changes which invol...

The cell cycle undergoes a sequence of changes which involve a period of growth replication of DNA, Followed by cell division. This sequence of changes is called cell cycle.

Zygote and fecundation formation occur in these plants, Q. How do zygote an...

Q. How do zygote and fecundation formation occur in these plants? Do these processes depend on water? The microsporangia in the male strobile rupture at the right time of the y

Define reaction for nelson-somogyi method, Define Reaction for Nelson-Somog...

Define Reaction for Nelson-Somogyi Method? The reagent used for enolization contains alkaline solution of cupric sulfate and Rochelle salt. Rochelle salt in the reagent prevent

According to their morphology how are bacteria classified, According to the...

According to their morphology how are bacteria classified? Bacteria present dissimilar morphological patterns. A bacterium can be classified into coccus, bacillus, vibrion or s

Explain the importance of the auxin, a) How is the milk production regulate...

a) How is the milk production regulated by hormones in human female? Define. b) Explain the importance of the auxin / cytokinin ratio in plant tissue culture.

Describe occurs from a physiological standpoint, Describe the occurs from a...

Describe the occurs from a physiological standpoint to the respiratory system when smoke is inhaled and exhaled. You will need to use other sources aside from your textbook.

Long term potentiation, It is a neural mechanism that plays a role in encod...

It is a neural mechanism that plays a role in encoding of new information. Encoding takes place as a result of changes in the strength of synapses among neurons in neuronal network

Comparison between metamorphosis in amphibians and insects, Comparison betw...

Comparison between Metamorphosis in Amphibians and Insects You may have realized that the metamorphic process in amphibians and insects show certain fundamental similarity. M

Mutual supplementation of protein with legume and milk, Define Mutual suppl...

Define Mutual supplementation of Protein with legume and milk? Improvement of cereal diets with legume and milk proteins: The proteins of poor diets based on different cereals

Endocrine glands of an insect, ENDOCRINE GLANDS OF AN INSECT - The endo...

ENDOCRINE GLANDS OF AN INSECT - The endocrine glands of cockroach are as follows. 1.       Intercerebral gland cells. These cells lie in the brain. They secrete brain horm

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd