Explain the equilibrium dialysis, Biology

Assignment Help:

Explain the Equilibrium Dialysis?

Equilibrium dialysis is a useful technique for studying the binding of a small uncharged solute species (a ligand) to a macromolecule. The macromolecule solution is placed on one side of a membrane through which it cannot pass, with a solution without the macro- molecule on the other side, and the ligand is allowed to come to transfer equilibrium across the membrane. If the same solute standard state is used for the ligand in both solutions, at equilibrium the unbound ligand must have the same activity in both solutions. Measure- ments of the total ligand molality in the macromolecule solution and the ligand molality in the other solution, combined with estimated values of the unbound ligand activity coefficients, allow the amount of ligand bound per macromolecule to be calculated.


Related Discussions:- Explain the equilibrium dialysis

Topic test, In what way do investigations build scientific knowledge?

In what way do investigations build scientific knowledge?

Observing where soil bacteria live, Observing where soil bacteria live ...

Observing where soil bacteria live Dig up a clover, alfalfa or soy-bean plant. Carefully increase all the soil from the roots and see if you can find the little white nodules o

Explain interactions of macrominerals with other nutrients, Explain Interac...

Explain Interactions of Macrominerals With Other Nutrients? Various nutrients interact with minerals thereby affecting their bioavailability. These interactions occur at differ

Define symptoms of overall wilting, If you observe symptoms of overall wilt...

If you observe symptoms of overall wilting, stunting, and thinning of the leaves, would you expect a foliar or root disease? Why?

Explain the digestion of carbohydrates, Explain the Digestion of carbohydra...

Explain the Digestion of carbohydrates? You are aware that 60-70% of energy is supplied by the dietary carbohydrates which are primarily present as polysaccharides (starch) fol

Evolutionary Biology-Genetic Drift, Imagine a population evolving by geneti...

Imagine a population evolving by genetic drift in which the frequency of allele K is 0.65. What is the probability that at some point in the future allele K will drift to a frequen

Transcription of a eukaryotic protein-coding gene, 1. Describe how the vari...

1. Describe how the various molecular mechanisms act to regulate transcription of the genes within the lactose operon of E. coli . 2. Transcription of a eukaryotic protein-codi

Explain diet history and nutrient intake, Explain Diet history and nutrient...

Explain Diet history and nutrient intake Diet history  and  nutrient intake : This  is used  to evaluate diet for nutrient or food intake. Common methods used include the 24-h

What are the types of proteins in metabolism of lens, What are the types of...

What are the types of proteins in metabolism of lens? Soluble Proteins Its synthesis takes place in the equatorial part on the surface of lens. a. Alpha crystallins

Describe the term - intellectual processes scales, Describe the term - Inte...

Describe the term - Intellectual Processes scales The 269 items are divided into 11 content scales, each of which may be administered individually. Since these scales contain v

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd