Explain the equilibrium dialysis, Biology

Assignment Help:

Explain the Equilibrium Dialysis?

Equilibrium dialysis is a useful technique for studying the binding of a small uncharged solute species (a ligand) to a macromolecule. The macromolecule solution is placed on one side of a membrane through which it cannot pass, with a solution without the macro- molecule on the other side, and the ligand is allowed to come to transfer equilibrium across the membrane. If the same solute standard state is used for the ligand in both solutions, at equilibrium the unbound ligand must have the same activity in both solutions. Measure- ments of the total ligand molality in the macromolecule solution and the ligand molality in the other solution, combined with estimated values of the unbound ligand activity coefficients, allow the amount of ligand bound per macromolecule to be calculated.


Related Discussions:- Explain the equilibrium dialysis

Necessity of transport in phloem, Necessity of Transport in Phloem Lea...

Necessity of Transport in Phloem Leaves produce photoassimilates and support various tissues including roots. The excess photoassimilates and metabolites stored in specialised

Polysaccharide sugars, Polysaccharide - polymer composed of monosaccharide...

Polysaccharide - polymer composed of monosaccharide monomers Starch - Energy storage in plants à straight (amylose) and branched (amylopectin) chains of α-glucose Glycogen

Sources of vitamin c, Sources of vitamin C It is found in many of th...

Sources of vitamin C It is found in many of the natural foods like fresh citrus fruits like limes, lemons and oranges are rich sources of vitamin C. The best and the chea

Management of chest injury, Management Establish and maintain airwa...

Management Establish and maintain airway. Anticipate need for incubation if respiratory distress evident.  Administer high humidified O 2 , using non-rebreather mask.

Pathology, Pathology: This concerned with the identification of different d...

Pathology: This concerned with the identification of different diseases, causative organs, their prevention and control methods. Pathology is a kind of study or diagnosis of diseas

Explain pullulan, Pullulan Pullulan is a water soluble edible microbial...

Pullulan Pullulan is a water soluble edible microbial polysaccharide consisting of Maltotriose units (α 1 → 6), as shown in  the figure 2.12. It is produced by yeast  Aureobasi

Productive use values, Productive use values This is the value of natu...

Productive use values This is the value of natural products harvested through commercial logging, agriculture or fisheries and medicines that generated products that are of co

Nutrition, mode of nutrition in tapeworm

mode of nutrition in tapeworm

How is excretion done in fishes, How is excretion done in fishes? Fish...

How is excretion done in fishes? Fishes have a pair of kidneys that filtrate the blood. Bony fishes excrete nitrogen as ammonia, NH 3 , (they are ammoniotelic) and cartilagino

Explain deteriorative changes in fats and oils, Explain Deteriorative chang...

Explain Deteriorative changes in fats and oils The food products undergo changes in flavour due to the chemical changes occurring in fats and oils present in them. The causativ

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd