Explain the equilibrium dialysis, Biology

Assignment Help:

Explain the Equilibrium Dialysis?

Equilibrium dialysis is a useful technique for studying the binding of a small uncharged solute species (a ligand) to a macromolecule. The macromolecule solution is placed on one side of a membrane through which it cannot pass, with a solution without the macro- molecule on the other side, and the ligand is allowed to come to transfer equilibrium across the membrane. If the same solute standard state is used for the ligand in both solutions, at equilibrium the unbound ligand must have the same activity in both solutions. Measure- ments of the total ligand molality in the macromolecule solution and the ligand molality in the other solution, combined with estimated values of the unbound ligand activity coefficients, allow the amount of ligand bound per macromolecule to be calculated.


Related Discussions:- Explain the equilibrium dialysis

Define glycolysis, Define glycolysis Glycolysis or the citric acid cy...

Define glycolysis Glycolysis or the citric acid cycle in which the direction of the reactions is well defined, the  interconversion reactions of the HMP pathway can function

Define mechanism for provide protection against colon cancer, Define Mechan...

Define Mechanisms for Provide protection against colon cancer? Several plausable mechanisms have been formulated by which fibre may provide protection against colon cancer. The

Arthropoda phylum, characteristic of spider that makes it the phylum arthro...

characteristic of spider that makes it the phylum arthropoda?

Explain animal fats, Animal Fats This group consists of depot fats from...

Animal Fats This group consists of depot fats from domestic land animals (e.g., lard and tallow), all containing large amounts of C16 and C18 fatty acids, medium amounts of uns

Algae, What is the basis of classification of algae

What is the basis of classification of algae

What is a terrestrial organism, Explain what is a terrestrial organism? ...

Explain what is a terrestrial organism? Ans) 'Terra' is the Latin word for earth. Thus, an animal that lives on the surface of the earth is known as terrestrial. This is the si

Neurophysics Assignment Help, What is Neurophysics Neurophysics is the ...

What is Neurophysics Neurophysics is the field of biophysics dealing with nervous system. Neurophysics covers a extensive spectrum of phenomena from molecular & cellular mechan

Vertebrate limb, Vertebrate Limb Earlier we know that how a series of...

Vertebrate Limb Earlier we know that how a series of sequential and coordinated interactions between different cell groups and tissues carry about the construction of a compl

Clinical presentation and investigation of hypertension, Any elevation in B...

Any elevation in BP is accompanied by increased risk of cardiovascular and cerebrovascular events and renal damage. The most distinctive pathological consequence

Strategies for genetic characterization and conservation, Strategies for ge...

Strategies for genetic characterization and conservation - During last 20 years concerted efforts have been made to create awareness among all the developmental organizations in th

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd