Explain the equilibrium dialysis, Biology

Assignment Help:

Explain the Equilibrium Dialysis?

Equilibrium dialysis is a useful technique for studying the binding of a small uncharged solute species (a ligand) to a macromolecule. The macromolecule solution is placed on one side of a membrane through which it cannot pass, with a solution without the macro- molecule on the other side, and the ligand is allowed to come to transfer equilibrium across the membrane. If the same solute standard state is used for the ligand in both solutions, at equilibrium the unbound ligand must have the same activity in both solutions. Measure- ments of the total ligand molality in the macromolecule solution and the ligand molality in the other solution, combined with estimated values of the unbound ligand activity coefficients, allow the amount of ligand bound per macromolecule to be calculated.


Related Discussions:- Explain the equilibrium dialysis

Explain basic function of the myelin sheath, Q. What is the function of the...

Q. What is the function of the myelin sheath? Do all axons present a myelin sheath? The function of the myelin sheath is to improve the speed and safety of the neural impulse t

What is prevailing wind direction, What is the prevailing wind direction in...

What is the prevailing wind direction in equatorial regions affected by the trade winds? a) The wind blows from east to west b) the wind blows from west to east.

Transcription in prokaryotes, As in several fields of molecular biology stu...

As in several fields of molecular biology studies of E. coli have gives the model for subsequent investigations of transcription in eukaryotic cells. The basic mechanisms by that t

What do you mean by high level of behavioral sophistication, Q What are the...

Q What are the few examples of beings of the phylum Arthropoda that present a high level of behavioral sophistication? Insects like some species of wasps, bees, termites and an

Explain cis-trans isomers, Explain Cis-trans isomers Atoms or groups a...

Explain Cis-trans isomers Atoms or groups are called  cis or  trans to one another when they project respectively on the same or on opposite sides of a reference plane  identi

What is cold-shortening, What is cold-shortening It is important to poi...

What is cold-shortening It is important to point out that over-effective chilling of hot carcasses can lead to toughness. If the temperature of the meat (muscles) can be reduce

Explain the phases of swallowing process in dysphagia, Explain the Phases o...

Explain the Phases of swallowing process in Dysphagia? i) Oral Phase: In this, food is placed in the mouth, mixed with saliva, chewed if required and formed into a bolus by t

Microbiology, Bacteria, mutations, recombination, evolution, and adaption, ...

Bacteria, mutations, recombination, evolution, and adaption, how are all these related; must be written in two sentences.

What is fermentation, What is Fermentation ? Fermentation is a process ...

What is Fermentation ? Fermentation is a process used by anaerobic organisms and certain cells of aerobic organisms, such as muscle cells deprived of oxygen, to obtain addition

Define classification of proteins on the basis of attributes, Define Classi...

Define Classification of proteins on the basis of attributes? Besides classifying proteins on the basis of soluble and insoluble, proteins have been further classified based on

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd