Explain the equilibrium dialysis, Biology

Assignment Help:

Explain the Equilibrium Dialysis?

Equilibrium dialysis is a useful technique for studying the binding of a small uncharged solute species (a ligand) to a macromolecule. The macromolecule solution is placed on one side of a membrane through which it cannot pass, with a solution without the macro- molecule on the other side, and the ligand is allowed to come to transfer equilibrium across the membrane. If the same solute standard state is used for the ligand in both solutions, at equilibrium the unbound ligand must have the same activity in both solutions. Measure- ments of the total ligand molality in the macromolecule solution and the ligand molality in the other solution, combined with estimated values of the unbound ligand activity coefficients, allow the amount of ligand bound per macromolecule to be calculated.


Related Discussions:- Explain the equilibrium dialysis

Why is rubella during gestation a threat to the fetus, Why is rubella durin...

Why is rubella during gestation a threat to the fetus? If occurring during gestation rubella is a dangerous disease due to the virus crosses the placenta and contaminates the f

Characteristic of ecotone, Characteristic of Ecotone A general charact...

Characteristic of Ecotone A general characteristic of ecotone is that it has sufficiently greater number of species and the diversity of most of the species at times is higher

Aceolomates, what are phylums included in aceolomates?

what are phylums included in aceolomates?

Differentiate soil horizons and mong the soil groups, Differentiate soil ho...

Differentiate soil horizons and mong the soil groups To make precise differentiation among soil horizons and among the soil groups, the field data on soil morphology are suppor

Preparation of patient for fasting blood sugar analysis, Preparation of Pat...

Preparation of Patient for Fasting Blood Sugar Analysis 1. Blood for fasting blood sugar (plasma glucose) analysis should be drawn after the individual has fasted overnight (8-

Write the meaning of dsme, Q. Write the meaning of DSME? Generally, it ...

Q. Write the meaning of DSME? Generally, it is observed that soon after being diagnosed with diabetes, the patient gets worried or get depressed. This is a stage where the pati

How you would accomplish cloning using of numbered steps, A restriction map...

A restriction map of plasmid and a human gene of identified sequence is demonstrated below. Your job is to clone the human liver cDNA for that gene than the start codon is closer t

How can the fermentation explain the origin of muscle cramps, How can the k...

How can the knowledge about fermentation explain the origin of muscle cramps and pains after intense physical exertion? A typical fermentation process because of oxygen scarcit

Determine major aspects of the mechanism of freeze drying, Determine Major ...

Determine Major Aspects of the Mechanism of Freeze Drying? The major aspects of the mechanism of freeze drying are:  (a) The removal of vapour from the subliming ice front w

Vascular tissues, VASCULA R TISSUES (= FLUID TISSUES) - The vascula...

VASCULA R TISSUES (= FLUID TISSUES) - The vascular tissues are of two types: blood and lymph.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd