Explain the equilibrium dialysis, Biology

Assignment Help:

Explain the Equilibrium Dialysis?

Equilibrium dialysis is a useful technique for studying the binding of a small uncharged solute species (a ligand) to a macromolecule. The macromolecule solution is placed on one side of a membrane through which it cannot pass, with a solution without the macro- molecule on the other side, and the ligand is allowed to come to transfer equilibrium across the membrane. If the same solute standard state is used for the ligand in both solutions, at equilibrium the unbound ligand must have the same activity in both solutions. Measure- ments of the total ligand molality in the macromolecule solution and the ligand molality in the other solution, combined with estimated values of the unbound ligand activity coefficients, allow the amount of ligand bound per macromolecule to be calculated.


Related Discussions:- Explain the equilibrium dialysis

Ganong''s potometer, What happens to the movement of the air bubble if the ...

What happens to the movement of the air bubble if the apparatus is kept in i. In the dark ii. In sunlight iii.In front of a fan

Chromosomes, Chromosomes  are the condensed, fibrillar, self- replicating g...

Chromosomes  are the condensed, fibrillar, self- replicating genetic structures of cells containing the cellular DNA which bears in its nucleotide sequence the linear array of gene

Explain about the cellular senescence - geriatric nutrition, Explain about ...

Explain about the Cellular senescence - Geriatric Nutrition? Aging brings about an irreversible state of growth arrest at the cellular level. Though the cells are viable and me

Explain the obesity - problems of older children nutrition, Explain the Obe...

Explain the Obesity - Problems of Older Children and Adolescent Nutrition? One of the major problems is raising obesity rates (upto 30%) in urban, well-to-do school children.Th

Saturated fatty acids required for dyslipidemia, Q. Saturated Fatty Acids r...

Q. Saturated Fatty Acids required for dyslipidemia? Saturated Fatty Acids (SFA): These are found mostly in animal fats as white marble-like solid at room temperature. Red meats

Define etiology and clinical features of alzheimer''s disease, Define the E...

Define the Etiology and Clinical Features of alzheimer's disease? The probable risk factors include a genetic basis, head injury, low education level, Down syndrome and mother

Explain post-lyme disease syndrome, Explain Post-lyme disease syndrome  ...

Explain Post-lyme disease syndrome  Some treated patients whose objective manifestations of Lyme disease have resolved with antibiotic treatment report subjective symptoms suc

Explain about pergingival regenerative therapy, Explain about Pergingival R...

Explain about Pergingival Regenerative Therapy In case of one stage implants being treated or when the prosthesis has to be maintained a  Pergingival Regenerative Therapy is d

Three phases into which the cell respiration is divided, What are the three...

What are the three phases into which the cell respiration is divided? The three phases of aerobic cell respiration are glycolysis, Krebs cycle and respiratory chain (also calle

Left coronary artery, The left coronary artery arises from the left posteri...

The left coronary artery arises from the left posterior aortic sinus. It runs to the left behind the pulmonary trunk and appears between left auricle and pulmonary trunk. In that p

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd