Explain the equilibrium dialysis, Biology

Assignment Help:

Explain the Equilibrium Dialysis?

Equilibrium dialysis is a useful technique for studying the binding of a small uncharged solute species (a ligand) to a macromolecule. The macromolecule solution is placed on one side of a membrane through which it cannot pass, with a solution without the macro- molecule on the other side, and the ligand is allowed to come to transfer equilibrium across the membrane. If the same solute standard state is used for the ligand in both solutions, at equilibrium the unbound ligand must have the same activity in both solutions. Measure- ments of the total ligand molality in the macromolecule solution and the ligand molality in the other solution, combined with estimated values of the unbound ligand activity coefficients, allow the amount of ligand bound per macromolecule to be calculated.


Related Discussions:- Explain the equilibrium dialysis

Explain the occurrence of vitamin B6, Explain the Occurrence of vitamin B 6...

Explain the Occurrence of vitamin B 6 Vitamin B 6 activity is attributed to the 3 compounds-pyridoxol (pyridoxine), pyridoxal and pyridoxamine, generally comprised in the vit

Metestrus - estrous cycle, Metestrus - Estrous cycle This occurs shortly...

Metestrus - Estrous cycle This occurs shortly after ovulation and is intermediate between estrus and diestrus. The period lasts for 10 to 14 hours and mating is usually not perm

What are the pancreas, Pancreas Insulin (β cells) Promotes glucose en...

Pancreas Insulin (β cells) Promotes glucose entry in many cells, and in this way controls carbohydrate metabolism. Glucagon (α cells) Increases synthesis and release of

The blood go after leaving the left atrium, To which heart chamber does the...

To which heart chamber does the blood go after leaving the left atrium? What is the valve that separates these compartments? The arterial blood that has come from the lungs to

Draw a diagram of transverse section of the spinal cord, Draw a diagram of ...

Draw a diagram of transverse section of the spinal cord showing reflex are and label the following parts: (i) Afferent nerve fibre (ii) Grey matter (iii) Motor nerve fibre

What is dip stick test, What is Dip Stick Test It is a rapid method of ...

What is Dip Stick Test It is a rapid method of detection of Ketone bodies which you can perform easily. Plastic strips impregnated with a buffered mixture of sodium nitropru

Bacterial artificial chromosome (bac), Bacterial artificial chromosome (BAC...

Bacterial artificial chromosome (BAC) is a chromosome-like structure, made by genetic engineering. BAC is a cloning vector able to carrying between 100 and 300 kilo bases of targe

Degrading monoglyceride, Degrade a monoglyceride that has an 18-carbon fatt...

Degrade a monoglyceride that has an 18-carbon fatty acid attached to it by Ester bonds. You will have to degrade the glycerol component followed by the fatty acid in presence of O2

#title.animal biodiversity., why obelia is considered to be of special inte...

why obelia is considered to be of special interest in zoology ass an animal showing intermediate grade of organisation

What is the molecular formula of glucose, What is the molecular formula of ...

What is the molecular formula of glucose? How can its structural formula be described? The molecular formula of glucose is C6H12O6. Structurally glucose is a hexagonal ring

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd