Explain the equilibrium dialysis, Biology

Assignment Help:

Explain the Equilibrium Dialysis?

Equilibrium dialysis is a useful technique for studying the binding of a small uncharged solute species (a ligand) to a macromolecule. The macromolecule solution is placed on one side of a membrane through which it cannot pass, with a solution without the macro- molecule on the other side, and the ligand is allowed to come to transfer equilibrium across the membrane. If the same solute standard state is used for the ligand in both solutions, at equilibrium the unbound ligand must have the same activity in both solutions. Measure- ments of the total ligand molality in the macromolecule solution and the ligand molality in the other solution, combined with estimated values of the unbound ligand activity coefficients, allow the amount of ligand bound per macromolecule to be calculated.


Related Discussions:- Explain the equilibrium dialysis

How would contrast single covalent bonds, 1. In modern blimps, the gas of c...

1. In modern blimps, the gas of choice used to inflate them is helium rather than hydrogen. Hydrogen would be lighter, but helium is safer. Compare and contrast the atomic structur

Do enzymes act better under acid or basic ph, Do enzymes act better under a...

Do enzymes act better under acid or basic pH? Most enzymes act in pH among 6 and 8, a range that corresponds to the general acidic level of cells and blood. There are enzymes,

Muscle cells, explain why it is essential that muscle cells, during anaerob...

explain why it is essential that muscle cells, during anaerobic respiration, convert pyruvic acid(pyruvate) into lactic acid, even though the conversion results in an accumulation

Explain about non-nutritive sweeteners, Q. Explain about Non-Nutritive Swee...

Q. Explain about Non-Nutritive Sweeteners? Non-Nutritive Sweeteners: These are characterized by an intense sweet taste. They are needed in small quantities and do not make any

Explain about pre-surgical prosthodontics, Pre-surgical Prosthodontics ...

Pre-surgical Prosthodontics Radiographic investigation: The radiologic investigations are critical to evaluate the bone quality and bone quantity. 1. Panoramic Radiograph

Define about oxygen molecule, How many pairs of electrons do the two oxygen...

How many pairs of electrons do the two oxygen atoms in an oxygen molecule share with each  other? Explain your answer. The oxygen atoms share two pairs of electrons, sinc

Define ailing and failing, Define ailing and failing Clinically unhealt...

Define ailing and failing Clinically unhealthy implants are classified as "ailing" or "failing". It is necessary to distinguish between an ailing versus a failing implant to de

Which of the processes results in the atp, Which of the processes results i...

Which of the processes results in the most ATP production within a cell?

In which type of animals does the placenta exist, In which type of animals ...

In which type of animals does the placenta exist? What is its main function? True placenta is present in placental mammals. The placenta is produced from the chorion of the

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd