Explain the equilibrium dialysis, Biology

Assignment Help:

Explain the Equilibrium Dialysis?

Equilibrium dialysis is a useful technique for studying the binding of a small uncharged solute species (a ligand) to a macromolecule. The macromolecule solution is placed on one side of a membrane through which it cannot pass, with a solution without the macro- molecule on the other side, and the ligand is allowed to come to transfer equilibrium across the membrane. If the same solute standard state is used for the ligand in both solutions, at equilibrium the unbound ligand must have the same activity in both solutions. Measure- ments of the total ligand molality in the macromolecule solution and the ligand molality in the other solution, combined with estimated values of the unbound ligand activity coefficients, allow the amount of ligand bound per macromolecule to be calculated.


Related Discussions:- Explain the equilibrium dialysis

Avian influenza (ai), A v i a n influenza (AI) One of only two 'Lis...

A v i a n influenza (AI) One of only two 'List A' diseases of poultry targeted for emergency disease control measures by OIE, avian influenza is a highly contagious viral d

Explain percutaneous interventions, Q. Explain Percutaneous Interventions ...

Q. Explain Percutaneous Interventions Over the last two decades, significant strides have been made in the field of Balloon Valvuloplasties both in terms of technique as well a

Comparison to healthy lungs, The nature of the sounds produced by percussio...

The nature of the sounds produced by percussion (tapping) a patient's chest can tell a physician a great deal about the condition of the organs within the thoracic cavity. Healthy,

Classify suture materials, Classify suture materials Sutures can broad...

Classify suture materials Sutures can broadly be classified into two kinds - Absorbable: will break down harmlessly in the body over time without intervention, Non-absor

What do you mean by subxiphoid long axis sweep, Q. What do you mean by Subx...

Q. What do you mean by Subxiphoid Long Axis Sweep? This sweep begins with keeping the transducer at subxiphoid region, positioning directly posterior with marker pointing towar

Fibre requirement in chronic diarrhoea, Q. Fibre requirement in chronic dia...

Q. Fibre requirement in chronic diarrhoea? Fibre: Insoluble fibre in the form of skins, seeds and structural plant materials should be strictly avoided to minimize on the irrit

Explain huntington disease gene, Research methods used in studying a geneti...

Research methods used in studying a genetic disease change with technological advances. These changes can be seen in Reading 22.1: Discovering the Huntington Disease Gene, which de

What is dyslipidaemia, What is Dyslipidaemia ? The results of epidemiol...

What is Dyslipidaemia ? The results of epidemiological studies, as well as trials with angiographic or clinical endpoints, confirm the importance of various lipid fractions in

Continuous wave doppler, In continuous wave Doppler, the transducer has tw...

In continuous wave Doppler, the transducer has two Doppler crystals, one to constantly transmit and one to continuously receive. As a result of this continuous mode of tra

Define proteins as structural elements and structural units, Define Protein...

Define Proteins as Structural Elements and Structural Units? The liver cell membrane analysis shows that this membrane contains 50-60% protein, 35% lipids and 5% carbohydrates.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd