Explain the equilibrium dialysis, Biology

Assignment Help:

Explain the Equilibrium Dialysis?

Equilibrium dialysis is a useful technique for studying the binding of a small uncharged solute species (a ligand) to a macromolecule. The macromolecule solution is placed on one side of a membrane through which it cannot pass, with a solution without the macro- molecule on the other side, and the ligand is allowed to come to transfer equilibrium across the membrane. If the same solute standard state is used for the ligand in both solutions, at equilibrium the unbound ligand must have the same activity in both solutions. Measure- ments of the total ligand molality in the macromolecule solution and the ligand molality in the other solution, combined with estimated values of the unbound ligand activity coefficients, allow the amount of ligand bound per macromolecule to be calculated.


Related Discussions:- Explain the equilibrium dialysis

Cerebellum, what is the work of cerebellum

what is the work of cerebellum

Show major signs of diabetes, Q. What are the three major signs of diabetes...

Q. What are the three major signs of diabetes? The three major signs of diabetes mellitus are known as the diabetic triad- polydipsia, polyuria and polyphagia. Polyuria is t

Define essentiality of nitrogen for growth of micro-organism, Define Essent...

Define Essentiality of Nitrogen for Growth of Micro-Organism? Nitrogen is an essential atom in certain cellular macromolecules, e.g. amino acids, purines, pyrimidines, enzymes,

Distinguish, distinguish between striated and cardic muscles

distinguish between striated and cardic muscles

Cranium, need images of cranium

need images of cranium

Explain a reason these cells are used instead of outer body, The cells lini...

The cells lining the inside of the cheek are frequently removed for making observations of basic cell structure. The cells are from stratified squamous epithelium. Explain a reason

Microbiology, advantages of spread plate method over pour plate method

advantages of spread plate method over pour plate method

Dermatitis, D i se a se s of Skin Der m a t i t i s De...

D i se a se s of Skin Der m a t i t i s Dermatitis is the inflammation of dermis and epidermis resulting in pain on palpation and erythema. E t iolo

Show major groups into which mammals are divided, Q What are the three majo...

Q What are the three major groups into which mammals are divided? The three groups into which mammals are divided are: monotremes (or prototherian that is platypus), placental

Digestive system, identify structural modifiactions of the stomach and smal...

identify structural modifiactions of the stomach and small intestine walls that enhance the digestive processes in these regions

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd