Explain the equilibrium dialysis, Biology

Assignment Help:

Explain the Equilibrium Dialysis?

Equilibrium dialysis is a useful technique for studying the binding of a small uncharged solute species (a ligand) to a macromolecule. The macromolecule solution is placed on one side of a membrane through which it cannot pass, with a solution without the macro- molecule on the other side, and the ligand is allowed to come to transfer equilibrium across the membrane. If the same solute standard state is used for the ligand in both solutions, at equilibrium the unbound ligand must have the same activity in both solutions. Measure- ments of the total ligand molality in the macromolecule solution and the ligand molality in the other solution, combined with estimated values of the unbound ligand activity coefficients, allow the amount of ligand bound per macromolecule to be calculated.


Related Discussions:- Explain the equilibrium dialysis

How is extracellular digestion related to cellular, How is extracellular di...

How is extracellular digestion related to cellular and tissue specialization? Several of specialized cells and tissues appeared with extracellular digestion to give enzymes and

What are the basic type of neurons, Q. According to the function of the tra...

Q. According to the function of the transmitted neural impulse which are the types of neurons? How different are the concepts of afference and efference of the neural impulse trans

How can you identify the signs and symptoms, Identify the signs and symptom...

Identify the signs and symptoms associated with death from mercury. Explain Please.

Reproduction, what is the difference between anaisogyami & oogyami?

what is the difference between anaisogyami & oogyami?

Brasseler endo extractor tube-endodontics principles, Brasseler Endo Extrac...

Brasseler Endo Extractor tube Insert H file buccally and lingually and remove silver by friction.If I can't remove use solvent to desolve cement If the tip of silver not appear

Disorders of pancreas, DISORDER S OF THE PANCREAS - (i ) Diabetes me...

DISORDER S OF THE PANCREAS - (i ) Diabetes mellitus (Hypoinsulinism) The insulin-dependent diabetes mellitus (IDDM) is caused by a failure of the Beta-cells to produc

Animals - slow moving waters, Animals - Slow Moving Waters Zooplankton...

Animals - Slow Moving Waters Zooplankton are common here and include an assemblage of protozoa and smaller crustacean, such as water flies, and copepods. Neuston occurring her

Sterigmatocystin, These mycotoxins are structurally and biologically relat...

These mycotoxins are structurally and biologically related to the aflatoxins, and like the latter, they cause hepatocarcinogenic activity in animals. Among the organisms that prod

Structural function - essential elements, Structural Function - Essential E...

Structural Function - Essential Elements The elements nitrogen, phosphorus and sulphur absorbed from the soil are essential components of amino acids and nucleotides. The othe

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd