Explain the equilibrium dialysis, Biology

Assignment Help:

Explain the Equilibrium Dialysis?

Equilibrium dialysis is a useful technique for studying the binding of a small uncharged solute species (a ligand) to a macromolecule. The macromolecule solution is placed on one side of a membrane through which it cannot pass, with a solution without the macro- molecule on the other side, and the ligand is allowed to come to transfer equilibrium across the membrane. If the same solute standard state is used for the ligand in both solutions, at equilibrium the unbound ligand must have the same activity in both solutions. Measure- ments of the total ligand molality in the macromolecule solution and the ligand molality in the other solution, combined with estimated values of the unbound ligand activity coefficients, allow the amount of ligand bound per macromolecule to be calculated.


Related Discussions:- Explain the equilibrium dialysis

Results of pericardial diseases, Results :  Pericardiectomy used to have a...

Results :  Pericardiectomy used to have a mortality of 10-15 per cent in the earlier era. At present it is around 3 to 5 per cent and does not approach 0 per cent even though it

Define triglycerides and atherogenesis, Triglyceride accumulation is not a ...

Triglyceride accumulation is not a feature of the atherosclerotic plaque but triglyceride-rich lipoproteins also contain cholesterol esters and it is likely that some of these are

Lyme disease, Lyme disease It is a metazoonoses caused by a spirochaete, B...

Lyme disease It is a metazoonoses caused by a spirochaete, Borrelia burgdorferi. The disease based on the specific symptoms, is also known as erythema migrans (ECM) or lyme  arthr

Endosperm, 1. What are the examples of helobial endosperm

1. What are the examples of helobial endosperm

How water content influence the rs content, How Water content Influence the...

How Water content Influence the RS content? The yield of RS formed in heat-moisture treatment is closely related to water content, which may be an inherent component of food or

Explain meningococcal disease, Meningococcal disease  Meningococcal vac...

Meningococcal disease  Meningococcal vaccine is recommended for adults and children >2 years old who are traveling to areas where epidemics are occurring, or to the "meningitis

Classical swine fever (hog cholera), Classical swine fever (hog cholera) ...

Classical swine fever (hog cholera) Swine fever, known as hog cholera or European Swine Fever is a highly infectious virus disease characterized by rapid spread and high morbi

Biology Question, Why is VeggieTales such a consistently high-quality progr...

Why is VeggieTales such a consistently high-quality program?

Financial accounting software, Financial Accounting Software An Ide...

Financial Accounting Software An Ideal Accounting Software should have the following features:  Purchase Details  Invoice Details  Purchase/Sale Return (Debit/Cre

What are the main types of waste, What are the main types of waste? The...

What are the main types of waste? The waste can be classified into main types, each of them carrying its own dissimilar environmental problem: organic waste, non recyclable was

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd