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Determination of Vitamin A
Vitamin A assay is carried out by chemical method. This method is called Carr Price Reaction.
Vitamin A and the carotenoids produce an intense blue colour upon addition of a saturated solution of antimony trichloride in anhydrous and alcohol-free chloroform. This reaction is called Carr Price Reaction and used to determine Vitamin A. The absorption maxima is around 620 nm.
In another method, absorption of vitamin A dissolved in isopropyl alcohol is determined at wavelengths of 310, 325 and 334 nm. This method is suitable for the determination of pure vitamin A (or of the ester after their saponification), such as oily solutions as well as in tablets, coated tablets, capsules and other formulations.
Vitamins necessity Vitamins are essential organic substances. They are micro nutrients and required in small quantities. They do not produce any energy by themselves.
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