Explain the decolourizing agent - ziehl-neelsen method, Biology

Assignment Help:

Explain the Decolourizing Agent - Ziehl-Neelsen Method?

Acid alcohol - a mixture of 95% ethanol and 3% HCI - is used for decolourization. Before decolourization, smear is allowed to cool to make waxy substances hardened. As primary stain is more soluble in cellular waxes than in decolourizing solution, acid-fast bacteria retain the primary stain. However, all non-acid fast bacteria loose primary stain because of the lack of cellular waxes.


Related Discussions:- Explain the decolourizing agent - ziehl-neelsen method

Explain first meiotic division, Q. What are the periods of the first meioti...

Q. What are the periods of the first meiotic division? Meiosis I is divided into metaphase I, prophase I, anaphase I and telophase I.

Why water has key participation in organic reactions, Water has key partici...

Water has key participation in organic reactions. What are examples of two types of organic reactions in which water is respectively incorporated or liberated in the products of th

What is the need of monitoring and surveillance, What is the need of Monito...

What is the need of Monitoring and Surveillance? Monitoring, we know, is the act of observing something and sometimes keeping a .record of it while surveillance is a repeated s

Enumerate different implant materials, Q. Enumerate different implant mater...

Q. Enumerate different implant materials? Biomaterials fall into four categories: metal and metal alloys, ceramics (carbon included in this group) synthetic polymers, and natur

rumen protection of nutrient (bypass nutrients) technology, Rumen protecti...

Rumen protection of nutrient (bypass nutrients) technology The amino acid and energy requirements of medium and high yielding cows and buffaloes are not fully met from the micr

Disorders of thyroid function, Disorders of Thyroid Function: The diso...

Disorders of Thyroid Function: The disorders of thyroid function may  lead to following problems:  Juvenile Hypothyroidism Hypothyroidism is one of the most common end

Explain therapeutic diets, Explain Therapeutic diets Therapeutic diets ...

Explain Therapeutic diets Therapeutic diets are adaptations of the normal or  regular diet.  In other words, it is a diet  for a patient suffering  from a  specific disease  su

Define absorption, Define Absorption, Storage and Elimination of niacin? ...

Define Absorption, Storage and Elimination of niacin? Nicotinic acid and nicotinamide are rapidly absorbed from the intestine rather than the stomach. At low concentrations, a

Corpus cardiacum - endocrine organs, Corpus Cardiacum - Endocrine Organs ...

Corpus Cardiacum - Endocrine Organs We before mentioned that corpus cardiacum (pl. corpora cardiaca) is a neurohemal organ in insects. It is gland of neural origin. They are c

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd