Explain the decolourizing agent - ziehl-neelsen method, Biology

Assignment Help:

Explain the Decolourizing Agent - Ziehl-Neelsen Method?

Acid alcohol - a mixture of 95% ethanol and 3% HCI - is used for decolourization. Before decolourization, smear is allowed to cool to make waxy substances hardened. As primary stain is more soluble in cellular waxes than in decolourizing solution, acid-fast bacteria retain the primary stain. However, all non-acid fast bacteria loose primary stain because of the lack of cellular waxes.


Related Discussions:- Explain the decolourizing agent - ziehl-neelsen method

Explain inhibitors, Explain Inhibitors Inhibitors: Citrate synthase i...

Explain Inhibitors Inhibitors: Citrate synthase is inhibited by ATP, NADH, succinyl CoA and acyl CoA derivative of fatty acids (fatty acyl CoA). The rate of the reaction  is

Molluscs., what are the economic and ecological importance of molluscs

what are the economic and ecological importance of molluscs

Antibiotics, ANTIBIOTICS - Chemicals produced by microorganism that ...

ANTIBIOTICS - Chemicals produced by microorganism that inhibit the growth of other microorganism. Such microorganism which produces the antibiotics are antibiont . Wh

What is the difference between transcription and translation, What is the d...

What is the difference between transcription and translation? Transcription is the name given to the formation of RNA molecules from an open DNA chain used as a template. Trans

Is the neural impulse generated by the stimulus, Q. Is the neural impulse g...

Q. Is the neural impulse generated by the stimulus that triggers the arch reflex restricted within the neurons of this circuit? This is obvious since the person that received t

Biological monitoring to validate that the sterilizer, Q. Biological monito...

Q. Biological monitoring to validate that the sterilizer? The use of biological monitors (spore tests) is the most reliable method to validate that the sterilizer is functionin

What is pressure support ventilation, What is Pressure Support Ventilation ...

What is Pressure Support Ventilation (PSV)? In a spontaneously breathing patient, each breath may be augmented with a preset level of pressure. The extent of support depends o

hormonal control of osmoregulation, This process involves the regulation o...

This process involves the regulation of the concentration of the body fluids through control of water content and salt content of the blood. If a person loses water from their bloo

Circulation, Circulation, Respiration and Excretion of Asteroidea Aste...

Circulation, Respiration and Excretion of Asteroidea Asteroids depend upon the circulation of coelomic fluid for the transport of gases and some nutrients. The blood vascular

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd