Explain the decolourizing agent - ziehl-neelsen method, Biology

Assignment Help:

Explain the Decolourizing Agent - Ziehl-Neelsen Method?

Acid alcohol - a mixture of 95% ethanol and 3% HCI - is used for decolourization. Before decolourization, smear is allowed to cool to make waxy substances hardened. As primary stain is more soluble in cellular waxes than in decolourizing solution, acid-fast bacteria retain the primary stain. However, all non-acid fast bacteria loose primary stain because of the lack of cellular waxes.


Related Discussions:- Explain the decolourizing agent - ziehl-neelsen method

Fundamental characters of embryonic development, FUNDAMENTAL CHARACTERS OF ...

FUNDAMENTAL CHARACTERS OF EMBRYONIC DEVELOPMENT 1 .       GAMETOGENESIS- Testes & ovaries are collectively called as gonads. Similarly sperms & ova are collectively calle

Explain pea plants in genetics, Explain pea plants in genetics? Pea Pl...

Explain pea plants in genetics? Pea Plants :   In pea plants, sperm are located in the pollen grains produced by the anthers of the flower. Eggs are formed in ovules, structur

What is coronary disease, What is coronary disease? Coronary disease, o...

What is coronary disease? Coronary disease, or coronary insufficiency, is a disease in which there is total or partial obstruction of one or more of the arteries that irrigate

Male reproductive system - accessory glands, ACCESSORY GLANDS - 1 .  ...

ACCESSORY GLANDS - 1 .      PROSTATE GLAND - One in number, 4-5 lobed, it forms 15-30% of semen.Citric acid, lipid, acid phosphatase spermin, fibrinolysine are present.

Extracellular signals, extracellular signals and their characteristics

extracellular signals and their characteristics

Is water a non-polar or a polar molecule, Q. Is water a non-polar or a pola...

Q. Is water a non-polar or a polar molecule? What is the consequence of that characteristic for the function of water as solvent? Ans. Water is made of two atoms of hydrog

Lumpy skin disease, Lumpy skin disease The disease, reported from Suda...

Lumpy skin disease The disease, reported from Sudan in 1970 and Egypt in 1988, is caused by a member of the Capripox virus. It affects cattle and is restricted to African coun

Problem of polarity in regeneration in planarians, Problem of Polarity in R...

Problem of Polarity in Regeneration in Planarians As in Hydra, flatworm regeneration as well appears to occur in a polar fashion. There seems to be ananterior-posterior gradie

Explain acylglycerols, Explain acylglycerols The most abundant class of...

Explain acylglycerols The most abundant class of food lipids is the acylglycerols, also known as glycerol esters of fatty acids, which dominate the composition of depot fats in

Phylem porifera, please give us this phylem''s example...

please give us this phylem''s example...

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd