Explain the decolourizing agent - ziehl-neelsen method, Biology

Assignment Help:

Explain the Decolourizing Agent - Ziehl-Neelsen Method?

Acid alcohol - a mixture of 95% ethanol and 3% HCI - is used for decolourization. Before decolourization, smear is allowed to cool to make waxy substances hardened. As primary stain is more soluble in cellular waxes than in decolourizing solution, acid-fast bacteria retain the primary stain. However, all non-acid fast bacteria loose primary stain because of the lack of cellular waxes.


Related Discussions:- Explain the decolourizing agent - ziehl-neelsen method

Pathogenesis of viruses, Pathogenesis of Viruses Viral diseases An...

Pathogenesis of Viruses Viral diseases An indicated before, viruses cause a number of infectious human diseases from minor ailments like common cold, to fatal disease, lik

Describe about the secondary prevention - food allergy, Describe about the ...

Describe about the Secondary Prevention - Food Allergy? Secondary allergic preventive measures will focus on: Initiating prospective surveillance of infants, young child

How computed tomography helps the doctors, Explain briefly how computed tom...

Explain briefly how computed tomography (CT) helps the doctors in pinpointing the defects in the patient's body

Scope of biology, SCOPE OF BIOLOGY- Present century is witnessing ex...

SCOPE OF BIOLOGY- Present century is witnessing explosion of information in modem biology directly related to life and health. Issues like global warming, pollution, populat

Origin of cell wall, Origin of Cell Wall - The cell wall is product ...

Origin of Cell Wall - The cell wall is product of cytoplasm. The cytoplasmic organelles such as endoplasmic reticulum, golgi apparatus play a very important role in the f

Inhibition might contribute to tumor formation, We now understand that muta...

We now understand that mutations that cause the inhibition of apoptosis are found in tumors. Because proliferation itself is not induced by the inhibition of apoptosis, explain how

Poisoning, Poisoning: Poisoning  is a common medical emergency  in chi...

Poisoning: Poisoning  is a common medical emergency  in childhood. In children under 5 years of  age essentially all poisoning are accidental. Nearly 75 per  cent  of  all poi

Agro industrial-selenium, Selenium Glutathione peroxidase (GSH-Px) was...

Selenium Glutathione peroxidase (GSH-Px) was the first selenium metalloenzyme identified.Glutathione peroxidase catalyzes the reduction of hydrogen peroxide and lipid h yp r o

Combined effects of drugs and alcohol, COMBINE D EFFECTS OF DRUGS AND ALCO...

COMBINE D EFFECTS OF DRUGS AND ALCOHOL - 1.       Alcohol + Barbiturates →  may lead to death . 2.       Alcohol + Antihistamines → Drowsiness 3.       Alcohol + Valium

What is the significance of elastic capsule chromatophore, What is the sign...

What is the significance of Elastic capsule chromatophore? Specialized pigmented cells on surface of the cephalopods that, by changing their shape, expose differing amounts of

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd