Explain the decolourizing agent - ziehl-neelsen method, Biology

Assignment Help:

Explain the Decolourizing Agent - Ziehl-Neelsen Method?

Acid alcohol - a mixture of 95% ethanol and 3% HCI - is used for decolourization. Before decolourization, smear is allowed to cool to make waxy substances hardened. As primary stain is more soluble in cellular waxes than in decolourizing solution, acid-fast bacteria retain the primary stain. However, all non-acid fast bacteria loose primary stain because of the lack of cellular waxes.


Related Discussions:- Explain the decolourizing agent - ziehl-neelsen method

Define water - important nutrient, Define Water - Important Nutrient? M...

Define Water - Important Nutrient? Macronutrients, you know, are those nutrients which are required in large amounts by our body namely, carbohydrates, fats and proteins. We st

Classification of living organisms – species, Classification of Living Orga...

Classification of Living Organisms – Species You have by now already come across the word 'species'. The word has a definite meaning in biology. Although the living world is v

Explain amylose, Amylose Amylose molecules have molecular weights rangi...

Amylose Amylose molecules have molecular weights ranging from 10 4 -20 6 . Amylose can form an extended shape (hydrodynamic radius 7-22 nm) but generally tends to wind up into

How are the male gametophytes formed in angiosperms, Q. How are the male ga...

Q. How are the male gametes and the male gametophytes formed in angiosperms? In the anthers of every stamen there are pollen sacs. Inside the pollen sacs there are microspore m

Ecology, Types of survivorship curves

Types of survivorship curves

What are hexoses, What are hexoses? What are some examples of hexoses with ...

What are hexoses? What are some examples of hexoses with important biological functions? Hexoses are carbohydrates made of six carbons. Glucose, fructose and galactose are inst

Explain about iceberg phenomenon, Q. Explain about Iceberg Phenomenon? ...

Q. Explain about Iceberg Phenomenon? Phenomenon means a fact or occurrence that appears. Let us see how iceberg phenomenon is applied to Diabetes Mellitus (DM). Cases of dia

Properties of isozymes, Properties of Isozymes Isozymes differ from  ...

Properties of Isozymes Isozymes differ from  each other not only in  the amino acid composition, they also have different electrophoretic property, thermolability, immunologi

Define the term - lamellac, Define the term - lamellac The inner membr...

Define the term - lamellac The inner membrane is folded into very closely packed membranes called the lamellac. The lamellae flatten out at regular intervals into structures c

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd