Explain the decolourizing agent - ziehl-neelsen method, Biology

Assignment Help:

Explain the Decolourizing Agent - Ziehl-Neelsen Method?

Acid alcohol - a mixture of 95% ethanol and 3% HCI - is used for decolourization. Before decolourization, smear is allowed to cool to make waxy substances hardened. As primary stain is more soluble in cellular waxes than in decolourizing solution, acid-fast bacteria retain the primary stain. However, all non-acid fast bacteria loose primary stain because of the lack of cellular waxes.


Related Discussions:- Explain the decolourizing agent - ziehl-neelsen method

Why immune mechanism of body of a patient gets suppressed, Name the disorde...

Name the disorder in which the immune mechanism of the body of a patient gets suppressed. Which pathogen is responsible for it? Give any two reasons of its transmission.

Functions of cell wall, FUNCTIONS OF CELL WALL 1.       Provides shape ...

FUNCTIONS OF CELL WALL 1.       Provides shape to plant cell rigidity to cells. 2.       Functions as a barrier to entry of pathogens into the cells. 3.       Provides pr

What do you understand by dimorphic nuclei, What do you understand by Dimor...

What do you understand by Dimorphic nuclei? Organization of the nuclear material typical of ciliates. There is always a single small micronucleus and either one large or many m

Define features of male and female ascaris lumbricoides, Define Features of...

Define Features of male and female ascaris lumbricoides? i)  Ascaris  lumbricoides is  commonly called  round worm. ii)  Body  is elongated, cylindrical,  tapering at  bot

What proportion of children with down syndrome found, What proportion of ch...

What proportion of children with Down syndrome do you expect when women with down syndrome have children with men who have 46 chromosomes? Justify answer

What food items include in clear liquid diet, What Food items include in cl...

What Food items include in clear liquid diet -  Clear, fat free soups/broths -  Light coffee, tea (without milk or cream) -  Strained fruit juices -  Tender coconut wa

Golgi complex, GOLGI COMPLEX ( GOLGI APPARATUS = GOLGI BODY) The Nobel ...

GOLGI COMPLEX ( GOLGI APPARATUS = GOLGI BODY) The Nobel laureate, Camilla  Golgi (1898)  discovered  the  presence of a special,  small group  of interconnecting  membranous  s

Explain about the bioavailability of minerals, Explain about the Bioavailab...

Explain about the Bioavailability of Minerals? Polyphenols can form complexes with metal cations through their carboxylic or hydroxylic groups, and thus interfere with the inte

Determine the sarcomere of a skeletal muscle, In the sarcomere of a skeleta...

In the sarcomere of a skeletal muscle, there are A. myosin molecules in the I band. B. both tropomyosin and myosin molecules in the region of the A band that is not in the H

Private or voluntary health insurance schemes, Normal 0 false ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd