Explain the decolourizing agent - ziehl-neelsen method, Biology

Assignment Help:

Explain the Decolourizing Agent - Ziehl-Neelsen Method?

Acid alcohol - a mixture of 95% ethanol and 3% HCI - is used for decolourization. Before decolourization, smear is allowed to cool to make waxy substances hardened. As primary stain is more soluble in cellular waxes than in decolourizing solution, acid-fast bacteria retain the primary stain. However, all non-acid fast bacteria loose primary stain because of the lack of cellular waxes.


Related Discussions:- Explain the decolourizing agent - ziehl-neelsen method

Explain total anomalous pulmonary, Explain Total Anomalous pulmonary Venous...

Explain Total Anomalous pulmonary Venous Connection (TAPVC) ? In this congenital anomaly there is no direct communication between the pulmonary veins and the left atrium. All

Areas affected in peptic ulcer, Q. Areas affected in peptic ulcer? The ...

Q. Areas affected in peptic ulcer? The areas affected in peptic ulcer (due to erosion) can be: 1. Lower part of the oesophagus. 2, stomach (lesser curvature - an urn, whe

Respiration, difference between anaerobic respiration and fermentation

difference between anaerobic respiration and fermentation

Coelentrates, classification of coelenterats?

classification of coelenterats?

Financial accounting software, Financial Accounting Software An Ide...

Financial Accounting Software An Ideal Accounting Software should have the following features:  Purchase Details  Invoice Details  Purchase/Sale Return (Debit/Cre

What is hydrogenqted fat, Q. What is Hydrogenqted Fat? All vanaspati pr...

Q. What is Hydrogenqted Fat? All vanaspati preparations are the result of hydrogenation of oils, where unsaturated fat is converted to saturated fat for its favour and long she

Explain antioxidants, Antioxidants  Antioxidants are the substances tha...

Antioxidants  Antioxidants are the substances that can delay onset, or  slow the rate of oxidation of autoxidizable materials. By virtue of this property,  they provide protect

Show photosynthesis process in algae and plants, Q. Which all are the livin...

Q. Which all are the living beings that carry out photosynthesis? Which is the cell organelle responsible for the absorption of light for the photosynthesis process in algae and pl

What are the results of chrchronic pulmonary thromboembolism, What are the ...

What are the Results of Chronic Pulmonary Thromboembolism? Results :  The reported mortality is between 5 and 24 per cent. On follow-up, the six-year survival in the best ce

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd