Explain the decolourizing agent - ziehl-neelsen method, Biology

Assignment Help:

Explain the Decolourizing Agent - Ziehl-Neelsen Method?

Acid alcohol - a mixture of 95% ethanol and 3% HCI - is used for decolourization. Before decolourization, smear is allowed to cool to make waxy substances hardened. As primary stain is more soluble in cellular waxes than in decolourizing solution, acid-fast bacteria retain the primary stain. However, all non-acid fast bacteria loose primary stain because of the lack of cellular waxes.


Related Discussions:- Explain the decolourizing agent - ziehl-neelsen method

Define nutrition support in postoperative nutritional care, Define Nutritio...

Define Nutrition Support in Postoperative Nutritional Care? Aggressive nutritional support is important to avoid postoperative complications and mortality. Routine postoperativ

Function of amino acids, FUNCTIO N OF AMINO ACIDS Proteins are poly...

FUNCTIO N OF AMINO ACIDS Proteins are polymers of amino acids. Glycine form porphyrin nucleus in chlorophyll and heme (= haem) proteins like haemoglobin and cytochrome

Inverse pcr, Inverse PCR is the variation of PCR which makes the amplifica...

Inverse PCR is the variation of PCR which makes the amplification of DNA segments of unknown sequence that flank DNA segments of the known sequence possible; in short, total DNA i

Define classification of proteins on the basis of attributes, Define Classi...

Define Classification of proteins on the basis of attributes? Besides classifying proteins on the basis of soluble and insoluble, proteins have been further classified based on

Natural versus artificial-man-made-environment, Natural versus Artificial (...

Natural versus Artificial (Man-made) Environment The environments discussed so far are, natural environments. In several instances man has greatly altered the natural condition

Of what importance are lactic acid fermentation to the cell, Of what import...

Of what importance are lactic acid fermentation and alcoholic fermentation to the cells that use these pathways? These pathways regenerate NAD, which the cells can use to keep

What is the importance of vitamin B12, What is the Importance of vitamin B ...

What is the Importance of vitamin B 12 Vitamin B 12 deficiency is manifest by the  symptoms of pernicious anaemia (Addison-Biermer's disease). An irreversible atrophy of the

Self-pollination - types of pollination, Self-Pollination - Types of Pollin...

Self-Pollination - Types of Pollination Self-pollination refers to the transfer of pollen grains from the anther to the stigma of the same flower. In chasmogamous flowers the

Describe asd closure and vsd closure in heart dieases, Describe ASD closure...

Describe ASD closure and VSD closure in heart dieases? ASD Closures : Small ASD's can be closed directly. Others will need a pericardial patch. Piimum ASD and Sinus Venosus A

Determine the term anapsid , Determine the term Anapsid ? The type of s...

Determine the term Anapsid ? The type of skull found in reptiles and seen today only in turtles. Behind the opening of the orbits the skull is solid and lacks temporal openings

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd