Explain the decolourizing agent - ziehl-neelsen method, Biology

Assignment Help:

Explain the Decolourizing Agent - Ziehl-Neelsen Method?

Acid alcohol - a mixture of 95% ethanol and 3% HCI - is used for decolourization. Before decolourization, smear is allowed to cool to make waxy substances hardened. As primary stain is more soluble in cellular waxes than in decolourizing solution, acid-fast bacteria retain the primary stain. However, all non-acid fast bacteria loose primary stain because of the lack of cellular waxes.


Related Discussions:- Explain the decolourizing agent - ziehl-neelsen method

Why nutrition is important for health, Why Nutrition is important for healt...

Why Nutrition is important for health Nutrition is one of the basic components of  life. It is an essential part of health care. You already know that good nutrition  is essent

Haustorial behavior of embryo sac, Haustorial Behavior of Embryo Sac ...

Haustorial Behavior of Embryo Sac There are instances in which the entire embryo sac may grow beyond the ovular tissue. The central cell may also form multicellular projectio

Prothoracic gland or ptc, Prothoracic Gland or PTC Prothoracic gland ...

Prothoracic Gland or PTC Prothoracic gland (PTC) or moulting gland and ecdysone or moulting hormone The 3 rd endocrine gland-prothoracic gland- is an irregular branching

Hunting and export - wildlife, Hunting and Export - Wildlife The hunti...

Hunting and Export - Wildlife The hunting and export of excessive numbers of certain animal species is another important factor leading to dangerous reductions in numbers. The

Endoplasmic reticulum, ENDOPLASMIC RETICULUM It is also known as Endosk...

ENDOPLASMIC RETICULUM It is also known as Endoskeleton of the cell, Endomembranous system of the cell. Garnier (1897) first observed them and called Ergastoplasm. E.R.

How we can do karyotype analysis, Why in the preparation of a karyotype ana...

Why in the preparation of a karyotype analysis is the use of a substance like colchicine interesting? Colchicine is a substance that disallows the formation of microtubules and

Explain spoilage of cereals and cereal products, Q. Explain Spoilage of Cer...

Q. Explain Spoilage of Cereals and Cereal Products? Cereals are the main source of energy to human beings. There are several varieties of cereals, of which wheat and rice are t

Define the food sources of thiamin, Define the Food Sources of Thiamin? ...

Define the Food Sources of Thiamin? Thiamin is present in many food products and depending on the amount of vitamin present, we have categorized the foods as rich, good or fair

Is gene a triplet of consecutive dna nucleotides, Is gene a triplet of cons...

Is gene a triplet of consecutive DNA nucleotides A gene isn't a triplet of DNA nucleotides with their respective nitrogen-containing bases, like CGT or AAG. The nucleotide trip

Ascitic fluid collection - specimen collection, Pleural, Pericardial, and A...

Pleural, Pericardial, and Ascitic fluid Collection:      The procedure is called paracentesis . If for pleural cavity is called thoracocentesis , and for pericardial cavity c

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd