Explain the decolourizing agent - ziehl-neelsen method, Biology

Assignment Help:

Explain the Decolourizing Agent - Ziehl-Neelsen Method?

Acid alcohol - a mixture of 95% ethanol and 3% HCI - is used for decolourization. Before decolourization, smear is allowed to cool to make waxy substances hardened. As primary stain is more soluble in cellular waxes than in decolourizing solution, acid-fast bacteria retain the primary stain. However, all non-acid fast bacteria loose primary stain because of the lack of cellular waxes.


Related Discussions:- Explain the decolourizing agent - ziehl-neelsen method

Classification of multicellular animals - coelom, Classification of Multice...

Classification of Multicellular Animals - Coelom We know earlier that the pseudocoel gave animals specific selective benefits. Among other things, this fluid-tilled space wor

Human respiratory system - thoracic cavity, THORACI C CAVITY - Thor...

THORACI C CAVITY - Thoracic cavity is air tight. On dorsal side vertebral column present. On ventral side sternum present. On lateral side 12 pairs ribs present. On p

What is isotope switching, It is conversion of antibody class to another re...

It is conversion of antibody class to another resulting from genetic rearrangement of heavy chain same region genes in B cells. Isotope switching is also known as class switching.

Differance between descending thoracic or abdominal aneurysm, Differance be...

Differance between Descending Thoracic or Abdominal Aneurysm ? Descending Thoracic Aortic and Thoraco-Abdominal Aneurysm :  The approach is through n left postero-lat

Show the symptoms of salmonellosis, Q. Show the Symptoms of salmonellosis? ...

Q. Show the Symptoms of salmonellosis? Symptoms: The susceptibility of humans varies with the species and strains of the organism and the total number of bacteria ingested. A

Phospholipids - conjugated lipids, PHOSPHOLIPIDS Most abundant lipid...

PHOSPHOLIPIDS Most abundant lipid present in cell membrane, also called membrane lipid. It is made up of lipid & phosphoric acid. The basic phospholipid is phosphat

Why his muscles so sore and feeling weak, Yesterday, when Eric returned hom...

Yesterday, when Eric returned home from jogging, he was breathing heavily, sweating profusely, and complained that his legs ached and felt weak. He took a drink of Gatorade and too

When the blood level of estrogen is high, Birth control pills maintain a hi...

Birth control pills maintain a high blood level of estrogen and progesterone. What is happening in the ovary when the blood level of estrogen is high? How is the uterus responding?

Why is ph regulation important for living beings, Why is pH regulation impo...

Why is pH regulation important for living beings? How do mineral salts participate in this regulation? The potential of hydrogen (pH) is a measure of the amount of hydrogen ion

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd