Explain the decolourizing agent - ziehl-neelsen method, Biology

Assignment Help:

Explain the Decolourizing Agent - Ziehl-Neelsen Method?

Acid alcohol - a mixture of 95% ethanol and 3% HCI - is used for decolourization. Before decolourization, smear is allowed to cool to make waxy substances hardened. As primary stain is more soluble in cellular waxes than in decolourizing solution, acid-fast bacteria retain the primary stain. However, all non-acid fast bacteria loose primary stain because of the lack of cellular waxes.


Related Discussions:- Explain the decolourizing agent - ziehl-neelsen method

Explain about the basidiomycota - fungi, Explain about the Basidiomycota - ...

Explain about the Basidiomycota - Fungi? Basidiomycota - Fungi in this division are called basidiomycetes or club fungi. Sexual structure is known as basidium, which is club-sh

What are the pentoses, Q. What are the pentoses? To what organic group do t...

Q. What are the pentoses? To what organic group do the pentoses belong? Are nucleotides formed of only one kind of pentose? Carbohydrates are Pentoses made of five carbons. Deo

Explain the air samplers - air sampling, Explain the Air Samplers - Air Sam...

Explain the Air Samplers - Air Sampling? Air Samplers, e.g., all glass impinger and the Andersen sieve samplers may be used. Volume of the air sampled is known. In all glass im

What are the advantages of the closed circulatory system, What are the adva...

What are the advantages of the closed circulatory system over the open circulatory system? The closed circulatory system is more efficient. As blood circulates only inside bloo

Explain the stages in taxonomic procedures - alpha taxonomy, Explain the St...

Explain the Stages In Taxonomic Procedures? Divided into three levels or phases: 1) Alpha (α) phase, 2) Beta (β) phase, 3) Gamma (γ) phase. Alpha Taxonomy The

Photosynthetic function, Photosynthetic function Structural organisa...

Photosynthetic function Structural organisation of the leaf is suitable to perform the process of photosynthesis. Hence leaf is called as a food factory. The leaf has a l

Denitrifying bacteria play in the nitrogen cycle, What part do (a) nitrifyi...

What part do (a) nitrifying, (b) nitrogen-fixing and (c) denitrifying bacteria play in the nitrogen cycle? a) Nitrifying bacteria in the soil change ammonia and other nitrogeno

What is the phenomenon known as red tide, What is the phenomenon known as "...

What is the phenomenon known as "red tide"? Which ambiental harms can it cause? Red tide is a phenomenon that happens when dinoflagellates (algae from the pyrrophyte group) pro

Describe what is circulatory support and inotropes, Describe what is Circul...

Describe what is Circulatory Support and Inotropes ? Colloid or Crystalloids: Hypotension after PGEl infusion is common. It is the result of relative intravascular volume deple

Explain exclusion diets, Explain Exclusion diets Exclusion diets: Speci...

Explain Exclusion diets Exclusion diets: Specific dietary exclusion becomes a necessity  in  case of  food allergy or food intolerance.  The therapeutic use of  such  diets req

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd