Explain the decolourizing agent - ziehl-neelsen method, Biology

Assignment Help:

Explain the Decolourizing Agent - Ziehl-Neelsen Method?

Acid alcohol - a mixture of 95% ethanol and 3% HCI - is used for decolourization. Before decolourization, smear is allowed to cool to make waxy substances hardened. As primary stain is more soluble in cellular waxes than in decolourizing solution, acid-fast bacteria retain the primary stain. However, all non-acid fast bacteria loose primary stain because of the lack of cellular waxes.


Related Discussions:- Explain the decolourizing agent - ziehl-neelsen method

What is prevailing wind direction, What is the prevailing wind direction in...

What is the prevailing wind direction in equatorial regions affected by the trade winds? a) The wind blows from east to west b) the wind blows from west to east.

Oreopithecus, Oreopithecus, a late rniocene primate had very close resembla...

Oreopithecus, a late rniocene primate had very close resemblances to those of gibbons, although the tooth structure showed that the animal was only a monkey and not a hominoid. Ano

Illustrate numerical taxonomy, Q. Illustrate Numerical Taxonomy? Taxono...

Q. Illustrate Numerical Taxonomy? Taxonomy today is, in many dctails, different from what it was a generation ago. The use of computers by taxonomists has established an intere

Define causes of vitamin a deficiency - inadequate diet, Define Causes of V...

Define Causes of Vitamin A Deficiency - Inadequate diet? A child is born with poor stores of vitamins and minerals due to maternal malnutrition. Diets of pregnant women are def

Classifications of carbohydrates based on number of aldehyde, Define Classi...

Define Classifications of carbohydrates based on number of aldehyde? Classification based on the presence of aldehyde or ketone group Carbon atoms Aldos

Define resolution - terminologies used in chromatography, Define Resolution...

Define Resolution - terminologies used in chromatography? Resolution is the effectiveness of the separation and is determined by two factors. These include: Distance

Explain about vascular cambium, How can the age of a tree be estimated from...

How can the age of a tree be estimated from the analysis of the rings present on a cross section of its stem? For the growth of the tree it is essential to have formation of ne

Explain gluten-free diet, Explain Gluten-free diet Gluten-free diet : ...

Explain Gluten-free diet Gluten-free diet : It is a diet recommended for the patients with gluten enteropathy. Gluten is present in wheat, rye, barley and oats. Thus, foods co

How does plasma membrane regulate, How does a plasma membrane regulate move...

How does a plasma membrane regulate movement of molecules into and out of a cell?

Classification of poisonous arthropods, what is the classification of poiso...

what is the classification of poisonous arthropods according to their mode of toxicity to human

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd