Explain the decolourizing agent - ziehl-neelsen method, Biology

Assignment Help:

Explain the Decolourizing Agent - Ziehl-Neelsen Method?

Acid alcohol - a mixture of 95% ethanol and 3% HCI - is used for decolourization. Before decolourization, smear is allowed to cool to make waxy substances hardened. As primary stain is more soluble in cellular waxes than in decolourizing solution, acid-fast bacteria retain the primary stain. However, all non-acid fast bacteria loose primary stain because of the lack of cellular waxes.


Related Discussions:- Explain the decolourizing agent - ziehl-neelsen method

Explain about extra low interstitial, Explain about Extra low Interstitial ...

Explain about Extra low Interstitial Extra low Interstitial (ELI) contains low levels of oxygen dissolved in interstitial sites in the metal. Lower amounts of oxygen and iron i

Technique of assessment of growth and development, TECHNIQUE OF ASSESSMENT ...

TECHNIQUE OF ASSESSMENT OF GROWTH AND DEVELOPMENT Measurement of physical growth in children is a key element in evaluation of the health status of children. Physical grow

Define prevention in the prevalence of overweight or obesity, Define Preven...

Define Prevention in the prevalence of overweight or obesity? It is believed that the increase in prevalence of obesity worldwide is more due to the environment that has become

Myocarditis, Myocarditis Myocarditis is inflammation of the myocardiu...

Myocarditis Myocarditis is inflammation of the myocardium, of the heart muscles. i) Causes Infection: . Wrus, bacteria, fungus, parasites Non-infection: Radi

Destarch the leaves of a potted plant, a) How would you destarch the leaves...

a) How would you destarch the leaves of a potted plant? b) How would you check that the destarching had been effective?   a) If a potted plant is kept in darkness for

Define precautions for preparation of phosphate buffers, Define Precautions...

Define Precautions for Preparation of Phosphate Buffers? 1. Glass electrodes should always be dried before and after measuring pH with a tissue paper. 2. The pipettes should

Fermentation, what is steroid production by microbial metabolism

what is steroid production by microbial metabolism

CORAL REEF., A COMPLETE SEMINAR TALK FOR CORAL REEF

A COMPLETE SEMINAR TALK FOR CORAL REEF

Rheumatic heart disease, Rheumatic heart disease was the predisposing cardi...

Rheumatic heart disease was the predisposing cardiac lesion for IE in 20 to 25 per cent. In patients with rheumatic heart disease, endocarditis occurs most frequently on the mitral

Explain the stages of alzheimer''s disease, Explain the Stages of alzheimer...

Explain the Stages of alzheimer's disease? Stage I - There is an increased forgetfulness, anxiety and depression. Associated nutrition related changes include difficulty in

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd