Explain the decolourizing agent - ziehl-neelsen method, Biology

Assignment Help:

Explain the Decolourizing Agent - Ziehl-Neelsen Method?

Acid alcohol - a mixture of 95% ethanol and 3% HCI - is used for decolourization. Before decolourization, smear is allowed to cool to make waxy substances hardened. As primary stain is more soluble in cellular waxes than in decolourizing solution, acid-fast bacteria retain the primary stain. However, all non-acid fast bacteria loose primary stain because of the lack of cellular waxes.


Related Discussions:- Explain the decolourizing agent - ziehl-neelsen method

Enumerate in detail about the cytoskeleton, Enumerate in detail about the C...

Enumerate in detail about the Cytoskeleton All eukaryotic cells have distinct shapes, and are also capable of assuming different shapes. 'The internal organelles of a cell are

Asexual reproduction, Asexual Reproduction Asexual reproduction is als...

Asexual Reproduction Asexual reproduction is also known agamic reproduction because there is no involvement of gametes in it. Asexual methods of reproduction are

What gases was the earths primitive atmosphere, Before the emergence of lif...

Before the emergence of life of what gases was the earth's primitive atmosphere constituted? The earth's primitive atmosphere was basically produced of methane, hydrogen, ammon

Frog, Frog fema reproductive system

Frog fema reproductive system

Nucleic acids, Deoxyribonucleic Acid (DNA) - polymer of nucleotides contai...

Deoxyribonucleic Acid (DNA) - polymer of nucleotides containing genetic information that codes for proteins Nucleotide - a monomer of DNA consisting of a ribose/deoxyribose sug

Define biosynthesis and secretion of thyroid hormones, Define Biosynthesis ...

Define Biosynthesis and Secretion of Thyroid Hormones? Histologically, the functional cells of the thyroid gland are arranged in follicles, which surround a central lumen conta

Pharmacological management, Once the decision to start drug treatment of hy...

Once the decision to start drug treatment of hypertension is made, the aim should be to provide 24 hour BP control, with agents that would encourage patient adherence. The patient

Define microorganism in fermentation foods process, Normal 0 fa...

Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4

Speciation, SPECIATION - Origin of new species: An isolated popula...

SPECIATION - Origin of new species: An isolated population of a species independently develops different types of mutations. The later accumulate in its gene pool. Afte

Dietary management of dyspepsia, Q. Dietary management of dyspepsia? Ke...

Q. Dietary management of dyspepsia? Keeping in mind the etiology, symptoms and complications of dyspepsia it must be clear that treatment and management of [his disorder does n

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd