Explain the decolourizing agent - ziehl-neelsen method, Biology

Assignment Help:

Explain the Decolourizing Agent - Ziehl-Neelsen Method?

Acid alcohol - a mixture of 95% ethanol and 3% HCI - is used for decolourization. Before decolourization, smear is allowed to cool to make waxy substances hardened. As primary stain is more soluble in cellular waxes than in decolourizing solution, acid-fast bacteria retain the primary stain. However, all non-acid fast bacteria loose primary stain because of the lack of cellular waxes.


Related Discussions:- Explain the decolourizing agent - ziehl-neelsen method

Explain neurologic disease, Explain Neurologic disease For patients wit...

Explain Neurologic disease For patients with facial nerve palsy alone, oral doxycycline or amoxicillin may be effective. Patients with other neurologic involvement, such as men

Define the food system in space, Define the Food System in space? Mercu...

Define the Food System in space? Mercury (1961-1963) astronauts had to eat bite-sized cubes, freeze dried powders, and semi liquids stuffed in aluminium tubes. For most asbonau

Intravenous route, Intravenous Route This route of administering drug...

Intravenous Route This route of administering drugs is becoming more popular in the paediatric practice. Purpose of this Route When rapid action is required.

Explain the staphylococcus - characteristics of bacteria, Explain the Staph...

Explain the Staphylococcus - Characteristics of Bacteria? Staphylococcus - It is gram positive, nonsporulating facultative anaerobic cocci present in grape-like clusters. These

Regulation of hmp pathway, Regulation of HMP Pathway The following fact...

Regulation of HMP Pathway The following factors play an important role in regulation of HMP pathway: a)  The first  reaction of this pathway catalysed by glucose-6-phosphate

Functions of the vascular system, Functions of the Vascular System The...

Functions of the Vascular System The major function of the circulatory system is to provide a transport mechanism for the blood flow to and from the heart. The vascular syste

Explain class oligochaeta - earthworms in details, Explain Class Oligochaet...

Explain Class Oligochaeta - Earthworms in details? This group of annelids includes the earthworms. Oligochaetes live in the soil or in fresh water habitats, and a few inhabit m

Prove thet nucleic acid is the transforming agent, Fredrick Griffith publis...

Fredrick Griffith published a series of experiments that collectively were dubbed "The Transforming Principle" in 1928. Which of the following stopped him from being able to declar

What is a species?, What is a species? The species is a set of living b...

What is a species? The species is a set of living beings able to cross among themselves generating fertile offspring. This concept though does not apply to individuals of ex

Describe the phylum echinodermata in details, Describe the Phylum Echinoder...

Describe the Phylum Echinodermata in details? The Phylum Echinodermata takes its name from two words of Greek origin: "echino," ; which means "prickle" or "spine," and "derma,"

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd