Explain the decolourizing agent - ziehl-neelsen method, Biology

Assignment Help:

Explain the Decolourizing Agent - Ziehl-Neelsen Method?

Acid alcohol - a mixture of 95% ethanol and 3% HCI - is used for decolourization. Before decolourization, smear is allowed to cool to make waxy substances hardened. As primary stain is more soluble in cellular waxes than in decolourizing solution, acid-fast bacteria retain the primary stain. However, all non-acid fast bacteria loose primary stain because of the lack of cellular waxes.


Related Discussions:- Explain the decolourizing agent - ziehl-neelsen method

Define the sun and solar drying method, Define the Sun and Solar Drying Met...

Define the Sun and Solar Drying Method? The make use of solar energy to preserve the food items is mainly the oldest form of food reservation and is still practiced today for f

How do schistosomes differentiate regarding sex separation, Q. How do schis...

Q. How do schistosomes differentiate regarding sex separation? The Schistosomes are dioecious that is the species has separated sexes, female and male individuals.

Name the molecules make active transport through membranes, Which are the m...

Which are the molecules that make possible active transport through membranes? Active transport is made by exact membrane proteins. These proteins are called "pumps" because

What are the synthetic auxins and what are their uses, What are the synthet...

What are the synthetic auxins and what are their uses? The Synthetic auxins like naphthalenic acid (NAA) and indolebutyric acid (IBA) are substances similar to IAA (a natural a

Classification and features of echinodermata, Classification with its Justi...

Classification with its Justification Kingdom Animalia Animals, multi-cellular organisms with cells that lack a cell wall, many capable of movement or movement of some o

Plant Taxonomy, Ecology and genetics as taxonomic units

Ecology and genetics as taxonomic units

West nile virus infection, West Nile virus infection West Nile virus (W...

West Nile virus infection West Nile virus (WNV) is a type strain of flaviviruses and is related to Japanese encephalitis group. The virus was first isolated from a woman in the

Explain therapy for diarrhoea, Q. Explain Therapy for diarrhoea? The th...

Q. Explain Therapy for diarrhoea? The therapy for diarrhoea thus consists of: 1. Determining the status of dehydration 2. Fluid management * Oral Rehydration Therapy (

Define the meaning of isomerism, Define the Meaning of Isomerism? All m...

Define the Meaning of Isomerism? All monosaccharides exhibit isomerism. An asymmetric carbon or chiral carbon contains four different groups attached to it. The formula 2 n de

What is moderate level of lactase activity, Q. What is Moderate level of La...

Q. What is Moderate level of Lactase activity? Intake of milk is restricted depending on the tolerance. Fermented and cooked form of milk should be preferred as it is better to

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd