Explain the decolourizing agent - ziehl-neelsen method, Biology

Assignment Help:

Explain the Decolourizing Agent - Ziehl-Neelsen Method?

Acid alcohol - a mixture of 95% ethanol and 3% HCI - is used for decolourization. Before decolourization, smear is allowed to cool to make waxy substances hardened. As primary stain is more soluble in cellular waxes than in decolourizing solution, acid-fast bacteria retain the primary stain. However, all non-acid fast bacteria loose primary stain because of the lack of cellular waxes.


Related Discussions:- Explain the decolourizing agent - ziehl-neelsen method

Would induction of CYP 2E1 raise your blood alcohol level?, If I take a dru...

If I take a drug that induced the synthesis of CYP 2E1 in my system, would that raise or lower my blood alcohol level after I drink a beer or wine (compared to if I hadn''t taken t

Explain about round worms, Which one of the following statements about cert...

Which one of the following statements about certain given animals is correct? 1. Round worms (Aschelminthes) are pseudocoelomates 2. Molluscs are acoelomates 3. Insects ar

Toxoplasmosis, Toxoplasmosis Toxoplasmosis, one of the important parasitic...

Toxoplasmosis Toxoplasmosis, one of the important parasitic zoonoses, occurs all over the world, and is caused by a protozoan parasite Toxoplasma gondii. Its definitive hosts are

Experiments of redi and pasteur of spontaneous generation, How did the expe...

How did the experiments of Redi and Pasteur refute the hypothesis of spontaneous generation? To refute the impulsive generation hypothesis many experiments were performed. The

Define types of dryers, Define types of Dryers? There are many other dr...

Define types of Dryers? There are many other dryer types available, such as; Osmotic Drying Impingement Drying Microwave and Dielectric Drying Superheated

How does the synthetic theory of evolution explain this fact, In hospitals ...

In hospitals where many tuberculosis patients are treated the population of the tuberculosis mycobacteria may be constituted of multiresistant (to antibiotics) strains. How does th

Explain procedure for the use of light microscope, Explain Procedure for th...

Explain Procedure for the use of Light Microscope? Now carry out the exercise following the steps enumerated herewith. 1. Place the microscopic slide with any specimen on th

Zooflagellates – protozoan, Zooflagellates – Protozoan Zooflagellates ...

Zooflagellates – Protozoan Zooflagellates are heterotrophs that are free living, commensals or symbiotic or parasitic in other animals. A number of species eg. Trichonynzpha a

How is the respiratory system of insects, Q. How is the respiratory system ...

Q. How is the respiratory system of insects with its independence between respiration and circulation related to the motor agility of some species of this arthropod class? Even

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd