Explain the decolourizing agent - ziehl-neelsen method, Biology

Assignment Help:

Explain the Decolourizing Agent - Ziehl-Neelsen Method?

Acid alcohol - a mixture of 95% ethanol and 3% HCI - is used for decolourization. Before decolourization, smear is allowed to cool to make waxy substances hardened. As primary stain is more soluble in cellular waxes than in decolourizing solution, acid-fast bacteria retain the primary stain. However, all non-acid fast bacteria loose primary stain because of the lack of cellular waxes.


Related Discussions:- Explain the decolourizing agent - ziehl-neelsen method

Urine sugar testing, Testing for urine sugars is not recommended for either...

Testing for urine sugars is not recommended for either diagnosis or monitoring of patients with diabetes. This is because a urine sugar is not a reliable test. When no facilities a

What are the cytochromes, Q. What are the cytochromes? Cytochromes are ...

Q. What are the cytochromes? Cytochromes are proteins of the interior mitochondrial membrane that are specialized in electron transfer and participate in the respiratory chain.

How do you verify that a certain gene encodes a certain prot, For homework,...

For homework, I had to prove that a certain catalyst was a protein, then tell how I would purify the protein, then tell how I would clone a human chromosomal region that contains t

Embryology and histology, Embryology: Embryology (from Greek words embryon...

Embryology: Embryology (from Greek words embryon which means unborn embryo and logia which means study) is a branch of science which deals with the development of an embryo starti

Define interaction of folate with vitamin b12, Define interaction of folate...

Define interaction of folate with vitamin B 12 ? Vitamin B 12 : For the conversion of folic acid to folinic acid, vitamin B 12 is required. Vitamin BIZ deficiency caus

Management of waste amalgam, Management of waste amalgam and wastes contain...

Management of waste amalgam and wastes containing mercury: As all of us know that amalgam is used for restorations very widely.An operator of a dental operation that produces w

Explain procedure for the use of light microscope, Explain Procedure for th...

Explain Procedure for the use of Light Microscope? Now carry out the exercise following the steps enumerated herewith. 1. Place the microscopic slide with any specimen on th

Introduction to transgenic animals, Transgenic Animal Over the centurie...

Transgenic Animal Over the centuries, animals with new combinations of genes have been produced using conventional breeding methods by means of careful selection of particular

What is sulphonylureas, Q. What is Sulphonylureas? Sulphonylureas: Tolb...

Q. What is Sulphonylureas? Sulphonylureas: Tolbutarnide is the mildest and its effect remains for 8 hours hence 2-3 doses need to be taken. Another drug chlorpropamide needs on

Define the neurological disorders of dysphagia, Define the neurological dis...

Define the neurological disorders of dysphagia? In neurological disorders, damage to cranial nerves can lead to weak and poorly co- ordinated tongue movements, which results in

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd