Explain the decolourizing agent - ziehl-neelsen method, Biology

Assignment Help:

Explain the Decolourizing Agent - Ziehl-Neelsen Method?

Acid alcohol - a mixture of 95% ethanol and 3% HCI - is used for decolourization. Before decolourization, smear is allowed to cool to make waxy substances hardened. As primary stain is more soluble in cellular waxes than in decolourizing solution, acid-fast bacteria retain the primary stain. However, all non-acid fast bacteria loose primary stain because of the lack of cellular waxes.


Related Discussions:- Explain the decolourizing agent - ziehl-neelsen method

What is physical accommodation, What is Physical Accommodation Physical...

What is Physical Accommodation Physical Accommodation is the actual physical deformation in the lens power. It is measured in diopters, e.g., if the focussing power of the eye

Anticoagulation, In patients with left ventricular failure and reduced ejec...

In patients with left ventricular failure and reduced ejection fractions, the risk of LV thrombus formation and systemic arterial embolization appears to be primarily in patients

Name the various test batteries, Name the various Test batteries Test ...

Name the various Test batteries Test batteries typically assess the following domains: General cognitive ability Language ability Visuoperceptual and constructi

Why in some cases is mitosis a synonym of reproduction, Why in some cases i...

Why in some cases is mitosis a synonym of reproduction? In some living beings asexual reproduction happens by many means: binary division, schizogony, budding, grafting, etc. I

Define the term - peroxisomes and glyoxisomes, Peroxisomes and Glyoxisomes ...

Peroxisomes and Glyoxisomes A number of metabolic reactions, such as, the oxidation of amino acids and lipids result in the production of hydrogen peroxide. As hydrogen peroxid

How many progeny flies are expected, How many progeny flies are expected to...

How many progeny flies are expected to have full wings and gray bodies?

Name the term for cancer of the blood, Which of the below terms is also cal...

Which of the below terms is also called "cancer of the blood" - an uncontrolled, greatly accelerated production of white cells. Is it: a) Leukemia b) Polycythemia c) sick

Illustrate about the chemical disinfection, Illustrate about the Chemical d...

Illustrate about the Chemical disinfection Sharp waste may cause injury and person may have diseases like Hepatitis B, Cand HIV/AIDS. Therefore it is important for to understa

Nutrition management goals for oesophagitis, Q. Nutrition Management Goals ...

Q. Nutrition Management Goals for oesophagitis? The objectives of nutritional care include the following: 1. Prevention of irritation of the inflamed oesophageal mucosa (in

Phototropism, wich variables are controlled when conducting a phototropism ...

wich variables are controlled when conducting a phototropism experiment

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd