Explain the decolourizing agent - ziehl-neelsen method, Biology

Assignment Help:

Explain the Decolourizing Agent - Ziehl-Neelsen Method?

Acid alcohol - a mixture of 95% ethanol and 3% HCI - is used for decolourization. Before decolourization, smear is allowed to cool to make waxy substances hardened. As primary stain is more soluble in cellular waxes than in decolourizing solution, acid-fast bacteria retain the primary stain. However, all non-acid fast bacteria loose primary stain because of the lack of cellular waxes.


Related Discussions:- Explain the decolourizing agent - ziehl-neelsen method

Maggots and murder, Plot the data as a suitable graph. Join the points with...

Plot the data as a suitable graph. Join the points with the best-fitting smooth curve.Temperature oC(5,10,11,13,17,19) Time taken for eggs to hatch/h 230 90 70 30 25

Protozoa, what are characteristics of flagellata class ?

what are characteristics of flagellata class ?

What is diabetes mellitus, Question 1 What is diabetes mellitus? Discuss b...

Question 1 What is diabetes mellitus? Discuss briefly its pathogenesis. Explain how would you diagnose this disorder Question 2 Discuss the following Glucose tolerance

Determine what the abnormal levels of afp signal, Determine what the abnorm...

Determine what the abnormal levels of AFP signal Abnormal levels of AFP may signal the following: Open neural tube Defect (ONTD) Down syndrome Other chromosoma1

Cell determination, Cell Determination Cell determination is a process...

Cell Determination Cell determination is a process through which portions of embryonic genome are selected for expression in particular embryonic cells. Determination to follo

Describe respiratory system , Describe Respiratory System? The respirat...

Describe Respiratory System? The respiratory system is responsible for the transport of oxygen and carbon dioxide from outside the body to and from the blood. This process is c

Common disorders of coronary heart diseases, Q. Common Disorders of Coronar...

Q. Common Disorders of Coronary Heart Diseases? We shall deal with different types of heart diseases, their- etiology, symptoms, complications, as well as, nutrition and dietar

Stratification - aquatic ecosystems, Stratification - Aquatic ecosystems ...

Stratification - Aquatic ecosystems Aquatic ecosystems also exhibit marked stratification. In lake and ocean ecosystems light penetration, temperature and availability of oxyg

What is the life cycle of a tapeworm, Q. What is the life cycle of a tapewo...

Q. What is the life cycle of a tapeworm? The Pregnant proglottids with taenia eggs are released together with human feces. If ingested by the intermediate hosts, bovineor swine

Role of hormones in aging, Role of Hormones in Aging Aging as well ref...

Role of Hormones in Aging Aging as well reflects the inefficiency of the endocrine system. A change in the hormonal secretions may contribute to the development of physical ai

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd