Explain the decolourizing agent - ziehl-neelsen method, Biology

Assignment Help:

Explain the Decolourizing Agent - Ziehl-Neelsen Method?

Acid alcohol - a mixture of 95% ethanol and 3% HCI - is used for decolourization. Before decolourization, smear is allowed to cool to make waxy substances hardened. As primary stain is more soluble in cellular waxes than in decolourizing solution, acid-fast bacteria retain the primary stain. However, all non-acid fast bacteria loose primary stain because of the lack of cellular waxes.


Related Discussions:- Explain the decolourizing agent - ziehl-neelsen method

Elaborate the internal vascular circulation of an eye, Elaborate the intern...

Elaborate the internal vascular circulation of an eye. Retinal vessels are derived from central retinal artery and are distributed within the inner two-third of retina. Outer o

Describe the the growth of the plants in the 2 groups, There has not been r...

There has not been rain for several months. A biologist is driving down the highway and notices that a large number of corn fields have corn that is wilted and short. She wonders i

Reducing pollution - conservation of wildlife, Reducing Pollution - Conserv...

Reducing Pollution - Conservation of wildlife As you already know, pollution of various kinds has affected the survival of living beings, particularly the wildlife in various

How to determines the actual availability of nutrients, How to determines t...

How to determines the actual availability of nutrients The amount of a particular nutrient determined analytically does not necessarily represent what is available but is merel

Explain arrayed library, Arrayed library: An individual primary recombinan...

Arrayed library: An individual primary recombinant clones (hosted in the phage, cosmid, YAC, or other vector) which are placed in two- dimensional arrays in microtiter dishes. Eac

Detailed explanation of effects of air pollution, On living organism  ...

On living organism           Air pollution causes following effects on living organism: (a)   Effects on humans: 1.      Carbon monoxide reduces the oxygen carrying cap

How we can measure perimplant pd, How we can measure perimplant PD Peri...

How we can measure perimplant PD Perimplant PD should be measured routinely during maintenance appointment with a less probing force (0.2 to 0.3 N) as the tissue is more sensit

Describe chronic mr murmur in mr murmur, Describe Chronic MR Murmur in MR M...

Describe Chronic MR Murmur in MR Murmur ? Characteristic: Pan systolic murmur, often loudest at apex and radiates to axilla, may also be audible in back and over the entire pre

Adaptive Radiations, Adaptive Radiations The organisms all the time te...

Adaptive Radiations The organisms all the time tend to establish harmony with their environment. This process, called adaptation, enables the organism to face the vagaries of

Define why the virginia opossum is considered a generalist, Define why the ...

Define why the Virginia opossum is considered a generalist and the koala is considered a specialist. The opossum feeds on almost anything, whereas the koala feeds only on the l

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd