Explain the decolourizing agent - ziehl-neelsen method, Biology

Assignment Help:

Explain the Decolourizing Agent - Ziehl-Neelsen Method?

Acid alcohol - a mixture of 95% ethanol and 3% HCI - is used for decolourization. Before decolourization, smear is allowed to cool to make waxy substances hardened. As primary stain is more soluble in cellular waxes than in decolourizing solution, acid-fast bacteria retain the primary stain. However, all non-acid fast bacteria loose primary stain because of the lack of cellular waxes.


Related Discussions:- Explain the decolourizing agent - ziehl-neelsen method

Topology and cell organ shapes, Topology and Cell Organ Shapes Topolog...

Topology and Cell Organ Shapes Topology is an abstract mathematical exercise that deals with the possible shapes and surfaces of an endlessly/infinitely elastic body. Most cel

Membrane perforation of sinus graft surgery, Membrane Perforation of sinus ...

Membrane Perforation of sinus graft surgery it is the most common complication during the sinus graft surgery resulting from the scoring of lateral access window for surgery an

Define interactions between volatile substances and proteins, Define Intera...

Define Interactions between Volatile Substances and Proteins? Flavour binding may involve adsorption at the surface of food or penetration to the food interiority by diffusion

What are the symptoms of pectic ulcer, Q. What are the Symptoms of pectic u...

Q. What are the Symptoms of pectic ulcer? Increased gastric tone and painful hunger contraction when stomach is empty. Hunger contraction 1-3 hours after meals is the main com

Most abundant form under which nitrogen is found in nature, Q. What is the ...

Q. What is the most abundant form under which nitrogen is found in nature? The major abundant nitrogen-containing molecule found in nature is molecular nitrogen (N2). The air i

Respiration, Can you define respiration in 5 oganisms

Can you define respiration in 5 oganisms

Nutrition in organisms, a project on different types of heterotrophic mode ...

a project on different types of heterotrophic mode of nutrition in organisms

Process of conducting system of the heart, Impulses reaching the AV node fr...

Impulses reaching the AV node from the atria are delayed a little as they pass through the trunk and crura. The impulses first reach the papillary muscles and their contraction clo

What is the function of cholecystokin, How is it produced and what is the f...

How is it produced and what is the function of cholecystokin in the digestive process? The fat level of the chyme detect in the duodenum stimulates the secretion of cholecystok

Protozoa, disadvanatge of protozoa

disadvanatge of protozoa

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd