Explain the decolourizing agent - ziehl-neelsen method, Biology

Assignment Help:

Explain the Decolourizing Agent - Ziehl-Neelsen Method?

Acid alcohol - a mixture of 95% ethanol and 3% HCI - is used for decolourization. Before decolourization, smear is allowed to cool to make waxy substances hardened. As primary stain is more soluble in cellular waxes than in decolourizing solution, acid-fast bacteria retain the primary stain. However, all non-acid fast bacteria loose primary stain because of the lack of cellular waxes.


Related Discussions:- Explain the decolourizing agent - ziehl-neelsen method

Explain the term health, Explain the term 'health' Mention any two ways of ...

Explain the term 'health' Mention any two ways of maintaining it. Why does a doctor administer tetanus antitoxin and not a tetanus vaccine to a child Injured in a roadside acci

Define the historical process of spatial processes, Define the Historical P...

Define the Historical Process of Spatial processes? In year 1952 Alan Turing developed his theory of the generation of pattern in systems of reaction diffusion equations, now k

What are the properties og gel, What are the properties og Gels? Polysa...

What are the properties og Gels? Polysaccharides, proteins or colloidal complex particles, such as caseinate micelles  form gels at levels of 10% or less. Gums, pectin and gela

Effect of insulin on metabolism of carbohydrate, The foods we take have thr...

The foods we take have three basic ingredients - carbohydrate, protein and fat. Insulin has profound effects on the metabolisms of carbohydrate, protein and fat. Metabolism helps o

Define protein requirement at different stages of life cycle, Define Protei...

Define Protein Requirement at Different Stages of Life Cycle? Methods of Estimating and Assessing Protein Requirements at Different Stages of Life Cycle In this section, we

The mechanism that ensures mendels first law, What is the mechanism that en...

What is the mechanism that ensures Mendel's First Law of segregation?

Biomolecules, indicator for testing for the presence of sugar

indicator for testing for the presence of sugar

Simple closed circulation and double closed circulation, Q. What is the dif...

Q. What is the difference between simple closed circulation and double closed circulation? Double closed circulation or Closed circulation is that in which the blood circulates

Explain environmental sampling - methods and techniques, Explain Environmen...

Explain Environmental Sampling - Methods and Techniques? Environmental sampling can be done for total microbial load or for some specific pathogens or spoilage organisms. The m

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd