Explain the decolourizing agent - ziehl-neelsen method, Biology

Assignment Help:

Explain the Decolourizing Agent - Ziehl-Neelsen Method?

Acid alcohol - a mixture of 95% ethanol and 3% HCI - is used for decolourization. Before decolourization, smear is allowed to cool to make waxy substances hardened. As primary stain is more soluble in cellular waxes than in decolourizing solution, acid-fast bacteria retain the primary stain. However, all non-acid fast bacteria loose primary stain because of the lack of cellular waxes.

Related Discussions:- Explain the decolourizing agent - ziehl-neelsen method

Explain about the mucor - fungi, Explain about the Mucor - Fungi? Muc...

Explain about the Mucor - Fungi? Mucor is present as a food contaminant. It resembles Rhizopus in life history and colonies appear just like Rhizopus. However, important diffe

Show the explanation passes a chi square test, You work with petunias and y...

You work with petunias and you have identified a single gene that causes their flowers to either be all pigmented (solid), or streaked with white. Depending on the genotype of this

Define principle of detection of metanil yellow, Define Principle of Detect...

Define Principle of Detection of Metanil Yellow? Non-permitted colours like lead chromate, metanil yellow, auramine, rhodamine B are used to brighten the foods especially spice

Protozoan, what are the phylum of kingdom protozoan

what are the phylum of kingdom protozoan

Explain gametophyte, "In these autotrophs, sporophyte is the dominant gener...

"In these autotrophs, sporophyte is the dominant generation. Gametophyte is also photosynthetic and not dependent on sporophyte for nutrition." These autotrophs are: a. P

What are the major types of lipids, Q. What are the major types of lipids? ...

Q. What are the major types of lipids? The main types of lipids are phospholipids, triglycerides (fats and oils), waxes and steroids.

Explain how will you perform ward radiography?, Question 1 Explain how ...

Question 1 Explain how will you perform ward radiography? Question 2 Discuss the radiographic projections recommended for imaging the first to third cervical vertebr

Enumerate the term diagnosis of epilepsy, State the diagnosis of epilepsy ...

State the diagnosis of epilepsy A diagnosis of epilepsy is usually confirmed by an EEG. In some epileptics, however, seizures are difficult to demonstrate in this way, except u

Explain the human body system and organs, Body systems and organs and their...

Body systems and organs and their functions The human body is a large structural unit made up of many smaller parts. There are four kinds of smaller units - cells, tissues, org

Liver function test, Liver function test should be done for all diabetic pa...

Liver function test should be done for all diabetic patients. This will help the doctor in the choice of drugs for the treatment of diabetes since liver abnormalities influence the

Write Your Message!

Name

Email id

Message

Verfication Code

User Account

All Pages