Explain the decolourizing agent - ziehl-neelsen method, Biology

Assignment Help:

Explain the Decolourizing Agent - Ziehl-Neelsen Method?

Acid alcohol - a mixture of 95% ethanol and 3% HCI - is used for decolourization. Before decolourization, smear is allowed to cool to make waxy substances hardened. As primary stain is more soluble in cellular waxes than in decolourizing solution, acid-fast bacteria retain the primary stain. However, all non-acid fast bacteria loose primary stain because of the lack of cellular waxes.


Related Discussions:- Explain the decolourizing agent - ziehl-neelsen method

Principles of polymerase chain reaction , The PCR   (polymerase   chai...

The PCR   (polymerase   chain   reaction)   is   an   extremely   simple   as   immensely powerful methods.  It permits enormous  amplification  of any particular  sequence of DNA

Left main coronary artery disease, Left Main Coronary Artery Disease (LMCA...

Left Main Coronary Artery Disease (LMCAD) : Stenosis of 50 per cent or more of the left main coronary artery is an indication. If or surgery. Results of medical management are ver

Define treatment and management of adverse food reactions, Define Treatment...

Define Treatment and Management of Adverse Food Reactions? Management of adverse food reactions involves diagnosing the problem followed by nutritional and medical care.

Explain vulvovaginal candidiasis, Vulvovaginal candidiasis  Vulvovagina...

Vulvovaginal candidiasis  Vulvovaginal candidiasis is not sexually transmitted, but is often found incidentally in women with STIs. Many remedies are available for vulvovaginal

Which functional groups ionization stabilized by resonance, In which of the...

In which of the following functional groups ionization is stabilized by resonance? Select one: a. Carboxyl b. Hydroxyl c. Amine d. Aldehyde e. All of the above

Placenta - human development, Placenta - Human Development We had said...

Placenta - Human Development We had said previous in the unit that in the second week of development a primitive uteroplacental circulation is established. Early in this stage

Phyletic lineage, explain in brief about phyletic lineage?

explain in brief about phyletic lineage?

Explain first meiotic division, Q. What are the periods of the first meioti...

Q. What are the periods of the first meiotic division? Meiosis I is divided into metaphase I, prophase I, anaphase I and telophase I.

Define some essential facts about the fats, Define some Essential facts abo...

Define some Essential facts about the Fats? 1) Fats are essential in diets to facilitate satiety, high-energy intakes, and absorption of fat-soluble vitamins and provide essent

What are the conventional signs used in genetic family trees, What are the ...

What are the main conventional symbols and signs used in genetic family trees? In genetic family trees the male sex is usually shown by a square and the female by a circle. Cro

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd