Explain the decolourizing agent - ziehl-neelsen method, Biology

Assignment Help:

Explain the Decolourizing Agent - Ziehl-Neelsen Method?

Acid alcohol - a mixture of 95% ethanol and 3% HCI - is used for decolourization. Before decolourization, smear is allowed to cool to make waxy substances hardened. As primary stain is more soluble in cellular waxes than in decolourizing solution, acid-fast bacteria retain the primary stain. However, all non-acid fast bacteria loose primary stain because of the lack of cellular waxes.


Related Discussions:- Explain the decolourizing agent - ziehl-neelsen method

Explain noncompetitive inhibition, Noncompetitive inhibition In  this ...

Noncompetitive inhibition In  this  type  of  inhibition, the inhibitor binds at  a site  on  the  enzyme  other  than catalytic site. As there is no competition between the s

Define classification of lipids, Define Classification of Lipids? Chemi...

Define Classification of Lipids? Chemically, lipids are the organic molecules poor in oxygen content, soluble in organic solvents but insoluble in water. They are classified as

State the young''s trichromatic theory, Young's Trichromatic Theory Ac...

Young's Trichromatic Theory According to Young's theory, three types of cones exist, each sensitive to a particular pigment-rythrolabe (red), chlorolabe (green), and cyanolabe

Metamorphosis, ME T AMORPHOSIS The differentiation results in the for...

ME T AMORPHOSIS The differentiation results in the formation of larva / infant. The formation of adult from the larva / infant is called as metamorphosis. The metamorph

Define iron status of the individual, Define Iron Status of the Individual ...

Define Iron Status of the Individual Lastly, iron status of the individual is a floury determinant of how much iron is absorbed. On a mixed diet with some haem iron, the overal

Define aim of neotaxonomy or systematic or biosystematics, Define Aim of Ne...

Define Aim of Neotaxonomy or Systematic or Biosystematics The aim of neotaxonomy or systematic or biosystematics is not only to describe, identify and arrange organisms in conv

Pathophysiology of leukemia, Pathophysiology   Leukocytes develop fiom ...

Pathophysiology   Leukocytes develop fiom stem cells located in the bone marrow. During  leukocyte maturation, cells become committed  to specific cell types and develop  throu

Non-muscular movement, NON-MUSCULA R MOVEMENT - 1 .      Streaming m...

NON-MUSCULA R MOVEMENT - 1 .      Streaming movement - In amoeba, cyclosis is common. 2 .      Pseudopodial - In leucocyte, macrophages, amoeboid movement take place

Phytoplankton stage - hydrarch, Phytoplankton Stage - Hydrarch In this...

Phytoplankton Stage - Hydrarch In this initial stage, the pond water is poor in nutrients and is devoid of much life. At this stage, the water is incapable of supporting large

What does acute disease mean, What does acute disease mean? An sharp di...

What does acute disease mean? An sharp disease is which does not last for a longer duration n has no weakness in the body

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd