Explain the decolourizing agent - ziehl-neelsen method, Biology

Assignment Help:

Explain the Decolourizing Agent - Ziehl-Neelsen Method?

Acid alcohol - a mixture of 95% ethanol and 3% HCI - is used for decolourization. Before decolourization, smear is allowed to cool to make waxy substances hardened. As primary stain is more soluble in cellular waxes than in decolourizing solution, acid-fast bacteria retain the primary stain. However, all non-acid fast bacteria loose primary stain because of the lack of cellular waxes.


Related Discussions:- Explain the decolourizing agent - ziehl-neelsen method

Illustrate mitral stenosis and pregnancy, Q. Illustrate Mitral Stenosis and...

Q. Illustrate Mitral Stenosis and Pregnancy ? Since mitral stenosis is often seen in young women, it is not uncommon to see young women with pregnancy complicated by mitral ste

Define the fact that we must eat to gain the energy, Which law of thermodyn...

Which law of thermodynamics explains the fact that we must eat to gain the energy to perform the functions of life, such as breathing? a. fourth law b. first law c. second law d

What is phototropism, What is phototropism? Phototropism is the movemen...

What is phototropism? Phototropism is the movement of plant structures in response to light. Phototropism might be positive or negative. Positive phototropism is that in which

Dietary considerations oesophagitis, Q. Dietary considerations oesophagitis...

Q. Dietary considerations oesophagitis? The dietary considerations for this condition are the same as in oesophag it is. The patient should be recommended to: • eat smaller

Define surgical procedures for cancer patient, Define Surgical Procedures f...

Define Surgical Procedures for Cancer Patient Major resection of the small bowel is not common. Resection of the ileum leads to certain physiological and nutritional problems.

Aortic allograft -biological valves, Aortic Allograft (Homograft) :  ...

Aortic Allograft (Homograft) :  In the earlier years fresh antibiotic preserved aortic allografts were tried. As the shelf life is limited, availability of all sizes at all t

Human biology, can i get a complete note to conceptualise human biology for...

can i get a complete note to conceptualise human biology for medical entrance preparation?

Explain the basic working of cellular functioning, Q Which is the biologica...

Q Which is the biological molecule that holds the genetic information that is transmitted hereditarily and controls the cellular functioning? The hereditary molecule that contr

How many different gene types are possible, In lab rats, hair color and tex...

In lab rats, hair color and texture are controlled by two genes, the A gene for color and the B gene for texture. in both of these genes, the alleles show no dominance to each othe

Polyarthritis, It is the most common (occurring in 75 per cent cases of ARF...

It is the most common (occurring in 75 per cent cases of ARF) manifestation of ARF. It involves large joints, it is typically fleeting in character shifting from one large joint t

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd