Explain the decolourizing agent - ziehl-neelsen method, Biology

Assignment Help:

Explain the Decolourizing Agent - Ziehl-Neelsen Method?

Acid alcohol - a mixture of 95% ethanol and 3% HCI - is used for decolourization. Before decolourization, smear is allowed to cool to make waxy substances hardened. As primary stain is more soluble in cellular waxes than in decolourizing solution, acid-fast bacteria retain the primary stain. However, all non-acid fast bacteria loose primary stain because of the lack of cellular waxes.


Related Discussions:- Explain the decolourizing agent - ziehl-neelsen method

Show fruiting bodies present in a few fungi, Q What are the fruiting bodies...

Q What are the fruiting bodies present in a few fungi? Fruiting bodies are structures made of hyphae that project radially from the superior portion of the peduncle of a few fu

Define the dietary sources and chemical forms, Define the Dietary Sources a...

Define the Dietary Sources and Chemical Forms? Isoflavones and coumestans are the most common compounds. Soybeans and soyfoods are the most important sources, containing approx

Define beaker - nutritional biochemistry, Define Beaker - Nutritional Bioch...

Define Beaker - Nutritional Biochemistry? It is used for storing a liquid to be used in a reaction and for dissolving a substance in a solvent to make its solution. A beaker sh

Formation of identical twins and fraternal twins, Describe the events whic...

Describe the events which lead to the formation of (a) identical twins, (b) fraternal twins.   (a) Identical twins are derived from the products of a one zygote which separa

Growth curve, typical growth curve in plants

typical growth curve in plants

Protozoa, locomotion in parameceum

locomotion in parameceum

What is the approximate ph of the salivary secretion, What is the approxima...

What is the approximate pH of the salivary secretion? Is it an acid or basic fluid? What are the main functions of saliva? The saliva pH is approximately 6.8. It is therefore a

Explain the Process of dna technology - Enzymes, Explain the Process of dna...

Explain the Process of dna technology Procedures involving recombinant DNA technology has been used to increase the yield of enzymes already produced by the microorganisms, e.

Explain the term of monosaccharides, Explain the term of Monosaccharides? ...

Explain the term of Monosaccharides? Monosaccharides :  The simplest carbohydrates are Monosaccharides, simple sugars. These generally have three to six carbon atoms, and con

Sperm which determines the sex of the offspring, a) In humans, is it the sp...

a) In humans, is it the sperm or the ovum which verifies the sex of the offspring? b) Give a reason for your answer.   (a) The sperm verifies the sex of the off

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd