Explain the decolourizing agent - ziehl-neelsen method, Biology

Assignment Help:

Explain the Decolourizing Agent - Ziehl-Neelsen Method?

Acid alcohol - a mixture of 95% ethanol and 3% HCI - is used for decolourization. Before decolourization, smear is allowed to cool to make waxy substances hardened. As primary stain is more soluble in cellular waxes than in decolourizing solution, acid-fast bacteria retain the primary stain. However, all non-acid fast bacteria loose primary stain because of the lack of cellular waxes.


Related Discussions:- Explain the decolourizing agent - ziehl-neelsen method

Define determinants of food security - food availability, Define Determinan...

Define Determinants of Food Security - Food Availability? It depends on the quantum and quality of crops, livestock, fishery and other food sources, as well as commercial impor

Explain the cell division, What is the cell division during the first stage...

What is the cell division during the first stage of the embryonic development called? How is this stage characterized? The cell division in the first stage of the embryonic de

Classification, what is the kingdom,phylum,class,age of gastropoda

what is the kingdom,phylum,class,age of gastropoda

Explain root amputation, Explain the Root amputation, Hemisectioning and Bi...

Explain the Root amputation, Hemisectioning and Bicuspidization Root amputation: removal of one or more roots of a multirooted tooth "upper teeth". Hemisectioning: surgic

Explain about glycemic index, Q. Explain about Glycemic Index? Although...

Q. Explain about Glycemic Index? Although the use of exchange lists is still popular for planning diabetic diets, it has been realized in recent years that in exchange lists th

Explain about the importance of vitamin e in human body, Explain about the ...

Explain about the Importance of Vitamin E in Human Body? Vitamin E is the generic term for tocopherols and tocopherols that have a Phenolic functional group on a chromane ring

Define reagent required and methodology for picric acid test, Define reagen...

Define reagent required and methodology for picric acid test? Reagents  - Sugar solutions of glucose, fructose, galactose, lactose, maltose, sucrose and starch. - Saturat

Why is cannibalism an inharmonious ecological interaction, Q. Why is cannib...

Q. Why is cannibalism an inharmonious intraspecific ecological interaction? In the cannibalism an individual eats other of the same species (occurs in some arachnids and insect

Define biosystematics - animal taxonomy, Define Biosystematics - Animal Tax...

Define Biosystematics - Animal Taxonomy Biosystematics is basically synonymous with systematic, though it places more emphasis upon genetic (concerning inheritance) and cytolog

Expression of variability, In the preceding section we emphasised the fact ...

In the preceding section we emphasised the fact that cvolution or the continuous adaptations to the environment can take place only when pheiiotypes exhibit variability. In the abs

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd