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Explain the Decolourizing Agent - Stain Technique?
95% ethanol is used as a decolourizing agent. It has two functions -
(1) It acts as protein - dehydrating agent, and
(2) It is a lipid solvent. The action of decolourizing agent depends on two factors -
(i) Concentration of lipids in cell wall.
(ii) Thickness of peptidoglycan layer.
In gram negatives, lipid content is high as compared to gram positives, so ethanol action increases the porosity of cell wall by dissolving the lipid layer in outer membrane. Peptidoglycan layer being thinner and less highly cross linked as compared to gram positives, is not able to retain the CV-I complex and cell become colourless. On the other hand, in gram positives, the pores become smaller due to dehydrating effect of the alcohol, so CV-I complex is retained by the thick highly cross linked peptidoglycan layer, and the cells remain purple. This step generates the differential aspect of the gram stain and is very critical.
Sulphur Cycle - Nutrient Cycles The sulphur cycle is mostly sedimentary except for a short gaseous phase. The large reservoir of sulphur is in the soil and sediment where it i
DIFFERENCE S BETWEEN MEDULLATED AND NON-MEDULLATED NERVE FIBRES 1. Medullated (Myelinated) Nerve Fibres Medullary sheath is present.
Vitamin E (DL-α-Tocopherol) DL-α-Tocopherol is a yellow to red-brown, clear, viscous oil almost without odour which decolourise when exposed to light and air. DL-α-Tocopherol
Chromium Chromium functions as a component of the glucose tolerance factor, which serves to potentiate the action of insulin. Adding low concentrations (0.02 to 1.0 mg/kg) of c
Explain the life cycle of Water? The water cycle , or hydrologic cycle, is one of the most important processes to living organisms on Earth. Consider the following facts:
Q. Clinical Features of aortic stenosis? Patients with congenital aortic stenosis usually present in childhood or adolescence. Those with rheumatic heart disease present durin
Phototropic curvature is the result of uneven distribution of : 1. Gibberellin 2. Phytochrome 3. Cytokinins 4. Auxin Auxin
What are the Cardiac muscles We know that the heart muscles (cardiac muscles) are not under the control of mind. The day we are born, it starts its activity and the day peopl
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In which areas of the globe is gymnosperm abundance noteworthy? These plants are the typical vegetation of cold regions as the taiga, or boreal forest, of the northern hemisph
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