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Explain the Bunsen Burner?
It is a type of gas burner that gives very hot flame by allowing air to enter at the base and mix with the gas. It is used -
(a) For sterilizing inoculating loop/needle/forcep etc.
(b) For sterilizing mouth of the flask, test tubes and other glass apparatus.
(c) At the time of culturing or transferring the microorganisms to avoid contamination.
Here the sterilization is done with dry heat. If bunsen burner is not available, spirit lamp can be used.
why is PCR carried out under sterille conditions?
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