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Explain the Basic Concept of Proteins?
Proteins, as we already knows by now are products of amino acids. Each molecule of protein is composed of many molecules of amino acids joined by peptide bond as can be seen in Figure.
Figure: Peptide bond formation
Proteins contain nitrogen in addition to carbon, hydrogen, oxygen and some other elements like sulphur. Thus determination of nitrogen is commonly used to determine the protein content of a sample. Proteins contain about 16% nitrogen and multiplying the nitrogen content by a factor of 6.25 gives us the amount of crude protein present in the given sample.
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Explain about the Biuret Method? The Biuret reaction was one of first colorimetric assays developed for protein estimation. It is most often used in applications requiring a fa
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