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Q. Explain Restricted fragment polymorphism?
This method makes use of a group of enzymes termed restriction enzymes which recognise particular sequence of DNA and act as "molecular scissors" to cut at the beginning and end of these sequences. When DNA from one individual is cut this way, and the resulting fragments are subjected to electrophoresis, the fragments become aligned by length enabling the recognition of a pattern. The analysis of patterns from different individuals permits an assessment of variation in their DNA sequences. Measuring the differing frequencies of the various patterns in a population or species can indicate the genetic diversity within the group.
System of classification The Enzyme Commission divided enzymes into 6 main classes, on the basis of the total reaction catalyzed. Each enzyme was assigned a code number; consis
You need to prepare 500 ml of a solution containing 10 mM Tris, 0.15 M NaCl and 1 mg/ml SDS. At your work disposal are stock solutions containing 1 M Tris, 2.5M NaCl and 10% (w/v)
Determine the case when starches treated with heat In the case of starches treated with heat in presence of acid or alkali, the alteration is a minor fragmentation; similarly,
Clothes : The concept of clothes might have started even before weaving, as an extension of the practice of carrying food hd implements about. Attachments with a convenient
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Blood Collection: Blood for analysis may be obtained from veins, arteries, or capillaries. Venous blood is usually the specimen of choice, and venipuncture is the method for
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