Explain requirement for simple staining of bacterial culture, Biology

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Explain Requirements for Simple Staining of Bacterial Cultures

Culture: 24 hours bacterial culture of E. coli and Bacillus on nutrient agar or in broth.

Reagents: Methylene blue or crystal violet

Equipments: Microscopic slides, inoculating loop, bunsen burner, staining tray, and Glassware microscope, lens paper, oil, permanent glass marker, blotting paper.

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