Explain procedure for test of presence of coliform bacteria, Biology

Assignment Help:

Explain Procedure for the test of Presence of Coliform Bacteria?

This test procedure is in continuation to the presumptive test procedure followed on last experiment. Now carry out this exercise following the steps enumerated herewith.

1. Label the EMB/Endo agar plates with the source of the water sample.

2. Streak the plates with the respective positive or doubtful presumptive broth culture using sterile inoculating loop.

3. Incubate all the culture plates in an inverted position for 24 hours at 37°C.

4. Observe the plates for the presence or absence of E.coli colonies.

Appearance of typical colonies with dark centres and metallic sheen indicates positive confirmed test, i.e., water is non potable.


Related Discussions:- Explain procedure for test of presence of coliform bacteria

Determine the term - leuconoid sponge, Determine the term - Leuconoid spong...

Determine the term - Leuconoid sponge. The most complex of the three different sponge architectures. Choanocytes are found in chambers, and there is not any spongocoel. Water e

Plants - slow moving waters, Plants - Slow Moving Waters Plant life is...

Plants - Slow Moving Waters Plant life is abundant in this habitat and includes rooted vascular plants such as pond weeds and grasses, firmly attached aquatic mosses and multi

Class of crustacea - branchiopoda, Class of Crustacea - Branchiopoda ...

Class of Crustacea - Branchiopoda These are small fresh water crustaceans. The trunk appendages are flattened and leaf like and are helpful for locomotion also respiration he

Multiple alleles, MULTIPLE ALLELES (i)         More than two alternativ...

MULTIPLE ALLELES (i)         More than two alternative forms (alleles) of a gene in a population occupying the same locus on a chromosome or its homologue are known as multiple

Seed structure, why mung beans has no endosperm while castor bean and maize...

why mung beans has no endosperm while castor bean and maize seed has?

What are the symptoms of brown-sequard syndrome, What are the Symptoms of B...

What are the Symptoms of Brown-Sequard syndrome Symptoms of Brown-Sequard syndrome usually appear after an affected individual experiences a trauma to the neck or back. First s

Define criteria for assessment of vitamin b12 status, Define Criteria for A...

Define Criteria for Assessment of Vitamin B 12 Status? Low serum or plasma levels of vitamin B 12 should be the first indication of poor  status and this could be confirmed

Conditions necessary for outbreak of staphylococcal food, Q. Conditions Nec...

Q. Conditions Necessary for Outbreak of staphylococcal food? The following conditions are necessary for an outbreak of staphylococcal food poisoning: 1. The food must conta

Implantable cardioverter defibrillator, Patients with aborted sudden death,...

Patients with aborted sudden death, hemodynamically unstable ventricular arrhythmias, and unexplained cardiogenic syncope are at high risk for fatal ventricular arrhythmias. If the

A protein is normally found inside of a lipid bilayer, A protein is normall...

A protein is normally found completely within the inside of a lipid bilayer. The protein likely has: a) Mostly hydrophobic side chains, pointed outwards, with the peptide backbo

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd