Explain procedure for sub-culturing of a culture, Biology

Assignment Help:

Explain Procedure for Sub-Culturing of a Culture

Now carry out the exercise following the steps given herewith:

1. Sterilize the inoculating loop and needle by heating to red hot.

2. Label all the tubes and nutrient agar plates.

3. Following the protocol given in section 4.3, transfer the E. coli and Bacillus sp. culture to:

(a) Nutrient agar tube,

(b) Nutrient slant and deep tube, and

(c) Nutrient broth.

4. Incubate the culture at 37°C and observe the growth after 24 hours.


Related Discussions:- Explain procedure for sub-culturing of a culture

Explain the metabolic pathways in the cornea, Explain the metabolic pathway...

Explain the metabolic pathways in the cornea. Metabolic Pathways: a. 88 per cent of glucose is utilized through glycolysis. b. Only 12 per cent of glucose utilized by

Explain the history of diet nnd, History of diet nnd or  tube feeding toler...

History of diet nnd or  tube feeding tolerance History of diet nnd or  tube feeding tolerance: The dietitian  presents  the patient's history, which may  include presence or hi

Genetics, GENETICS 1.         The branch genetics started in 1900 after...

GENETICS 1.         The branch genetics started in 1900 after rediscovery of Mendel's work. 2.         Gregor Johann Mendel is called Father of genetics. 3.         The t

What is root cap, What is root cap? Root cap is a protective structure ...

What is root cap? Root cap is a protective structure located in the tip of the growing root. It defends the meristematic tissue of the root forming a cap that surrounds the tip

What is intra oral and extra oral, What is Intra oral and Extra Oral A...

What is Intra oral and Extra Oral A) Intra oral a) Frontal in occlusion b) Left and right occlusion (Using intra oral photography mirror) c) Occlusal view maxillary

Heat exchangers, Normal 0 false false false EN-IN X-N...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Basic properties to proteins, Define Basic Properties To Proteins, To Be a ...

Define Basic Properties To Proteins, To Be a Good Foaming Agent? A protein must: a) Be able to rapidly absorb at the air-water interface during whipping, b) Undergo rapid

Explain about the stilbenes, Explain about the Stilbenes? Stilbenes con...

Explain about the Stilbenes? Stilbenes contain 3 phenyl compounds connected by a 2-carbon methylene bridge and occur in nature in restricted distribution. In plants, they act a

What is an evolutionary explanatory hypothesis, Q. What is an evolutionary ...

Q. What is an evolutionary explanatory hypothesis for the secretion by the heart of a hormone that regulates the renal function? Which is that hormone? The renal regulator horm

Artificial inspiration, Artificial Inspiration : a. After artificial exp...

Artificial Inspiration : a. After artificial expiration, the rescue worker should slowly release the pressure on the back of the victim. b. This will cause the chest cavity t

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd