Explain procedure for sub-culturing of a culture, Biology

Assignment Help:

Explain Procedure for Sub-Culturing of a Culture

Now carry out the exercise following the steps given herewith:

1. Sterilize the inoculating loop and needle by heating to red hot.

2. Label all the tubes and nutrient agar plates.

3. Following the protocol given in section 4.3, transfer the E. coli and Bacillus sp. culture to:

(a) Nutrient agar tube,

(b) Nutrient slant and deep tube, and

(c) Nutrient broth.

4. Incubate the culture at 37°C and observe the growth after 24 hours.


Related Discussions:- Explain procedure for sub-culturing of a culture

Gonial apospory and somatic apospory, Gonial Apospory and Somatic Apospory ...

Gonial Apospory and Somatic Apospory Gonial Apospory There is no meiosis. The megaspore mother cell enlarges; a small vacuole appears above and below the nucleus. The c

Components of ecosystem, An ecosystem consists of two types of components: ...

An ecosystem consists of two types of components: (a)   Abiotic components: This includes the non-living components of the ecosystem. These are physical and chemical fact

Animal nutrition, briefly explain the following feeding mechanisms in holoz...

briefly explain the following feeding mechanisms in holozoic animals a) filter feeding b) fluid feeding c)deposit feeding

How does consecutive protein production occur in translation, How many of t...

How many of the same proteins are made at the same time by each ribosome in the translation of one mRNA molecule? How does consecutive protein production occur in translation?

Describe about investigative tools and their optimal use, Describe about In...

Describe about Investigative Tools and their Optimal Use ? The investigative tools that are available for diagnosis of CHD include ECG, chest X-ray, 2D and Doppler echocardiog

Deficiency diseases-rickets, Rickets Rickets is a complex mineral defic...

Rickets Rickets is a complex mineral deficiency disease of young, growing animals. It is characterized by defective mineralization of growing bones, persistence of hypertrophic

Different from the interphase of meiosis, Q. Is the interphase of mitosis d...

Q. Is the interphase of mitosis different from the interphase of meiosis? The interphase mitosis that proceeds is similar to the interphase that precedes meiosis. In them the m

Mode of nutition, What is the mode of nutrition of Ascaris?

What is the mode of nutrition of Ascaris?

Nutrition, heterotrophic mode nutrition plants

heterotrophic mode nutrition plants

Define chagas and arrhythmogenic right vent cardiomyopathy, Chagas' Cardiom...

Chagas' Cardiomyopathy It is common in south and Central America and is caused by Trypanosoma cruzi. Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) It is mark

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd