Explain procedure for simple staining of bacterial cultures, Biology

Assignment Help:

Explain Procedure for Simple Staining of Bacterial Cultures?

Now carry out the exercise following the steps given herewith:

(1) Prepare thin bacterial smear on clean glass slide.

(2) Fix the bacterial smear on glass slide by heat fixation.

(3) Place the slide on the staining tray.

(4) Flood the smear with a staining reagent for appropriate exposure time.

(5) Remove the excess stain by gentle washing with tap water. The slide should be held parallel to the stream of water to avoid removal of microorganisms from slide.

(6) Blot dry the slide.

(7) Examine the dried slide under microscope in oil immersion (100x). First fix the slide at 10x and then move to 100x. (Oil immersion).


Related Discussions:- Explain procedure for simple staining of bacterial cultures

What electrocardiogram results show a shortened qt interval, Electrocardiog...

Electrocardiogram results show a shortened QT interval and an increase in the P-R interval (i.e., decreased duration of action potential and decreased conduction velocity). Laborat

Explain about the solar dryers, Explain about the Solar Dryers? There a...

Explain about the Solar Dryers? There are so many different designs for solar dryers, these range from simple convection dryers, cabinet dryers, shelf dryers to more complicate

How are genetic diseases classified, How are genetic diseases classified? ...

How are genetic diseases classified? The Genetic diseases classify into chromosomal abnormalities and genetic mutations. Between chromosomal abnormalities there are the aneu

Describe tranposition of great arteries intact ventricular, Describe tranpo...

Describe tranposition of great arteries with intact ventricular septum? Transposition of great arteries with intact ventricular septum (or small VSD): There is usually inadequa

Osmosis: Hypertonic Solution, If a cell were placed in a hypertonic solutio...

If a cell were placed in a hypertonic solution what could it do to protect itself?

Explain about the browning reactions, Explain about the Browning Reactions?...

Explain about the Browning Reactions? The Browning Reactions are complex reactions which occur when foods are processed. In some the brown flavour is highly desirable and is in

What is erythroblastosis fetalis, It is a hemolytic disease, which makes in...

It is a hemolytic disease, which makes in newborn. Maternal IgG antibodies cross the placenta and destroy the red bleed cells. This develops when an Rh+ expresses an Rh antigen on

Explain phosphofiuctokinase-i, Phosphofiuctokinase-I Phosphofiuctokina...

Phosphofiuctokinase-I Phosphofiuctokinase-I  is activated by AMP and  inhibited by ATP and citrate. When ATP is utilized in energy requiring process, the concentration ofAMP

Hybridization, Hybridization  is the reaction by which pairing of the compl...

Hybridization  is the reaction by which pairing of the complementary strands of nucleic acid happens. DNA is usually double-stranded, and when these strands are separated form each

What are the major plant tissues, What are the major plant tissues that for...

What are the major plant tissues that form the rings observed on stem sections of some trees? The rings observed on a stem cross section of the dicot trees are made of conducti

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd