Explain procedure for simple staining of bacterial cultures, Biology

Assignment Help:

Explain Procedure for Simple Staining of Bacterial Cultures?

Now carry out the exercise following the steps given herewith:

(1) Prepare thin bacterial smear on clean glass slide.

(2) Fix the bacterial smear on glass slide by heat fixation.

(3) Place the slide on the staining tray.

(4) Flood the smear with a staining reagent for appropriate exposure time.

(5) Remove the excess stain by gentle washing with tap water. The slide should be held parallel to the stream of water to avoid removal of microorganisms from slide.

(6) Blot dry the slide.

(7) Examine the dried slide under microscope in oil immersion (100x). First fix the slide at 10x and then move to 100x. (Oil immersion).


Related Discussions:- Explain procedure for simple staining of bacterial cultures

Define vitamins and minerals needs in nutrient requirement, Define Vitamins...

Define Vitamins and Minerals needs in Nutrient Requirement and Dietary Management Although the exact requirements are not known, it is generally recommended to give plenty of f

Haptotaxis - modes of cell movement, Haptotaxis - Modes of Cell Movement ...

Haptotaxis - Modes of Cell Movement Haptotaxis considers to the directed movement of cells in response to a concentration gradient of an adhesive molecule that might be presen

Results-ross operation--surgical techniques, Results: The risk of isolate...

Results: The risk of isolated aortic valve replacement is 3-4 per cent. The long term survival for 5, 10 and 15years is 75 per cent, 60 per cent and 40 per cent respectively. The

Explain gum karaya, Gum Karaya Gum karaya (sterculia gum) is the...

Gum Karaya Gum karaya (sterculia gum) is the dried gummy exudate from Sterculia urens Roxburgh and other species of Sterculia (Family: Sterculiaceae) or from Cochlosperm

Carbohydrate requirement during congestive cardiac failure, Q. Carbohydrate...

Q. Carbohydrate requirement during congestive cardiac failure? While the quantity of carbohydrate remains almost the same as per the RDI i. e, 60% of the total energy, the qual

Hetero trophic mode of nutrition, what is heterotroophic mode of nutrition?...

what is heterotroophic mode of nutrition?give some information about heterotrophic mode of nutrition?

Population growth, Increase in population size is known as population growt...

Increase in population size is known as population growth. It depends upon number of persons added to the population and number of persons lost from the population. Addition in pop

Importance of counselling for diabetic patient, Q. Importance of counsellin...

Q. Importance of counselling for diabetic patient? Diabetes is a life-long illness. Life style modification and precautions taken by the patient can prevent complications and i

Determine the principle of brain and behaviour, Determine the Principle of ...

Determine the Principle of Brain and Behaviour A second principle is that insight into children's adaptation can be gained through an analysis of brain behaviour relationships.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd