Explain procedure for performing the completed coliform test, Biology

Assignment Help:

Explain Procedure for Performing the Completed Coliform Test?

Completed test is the last step in the Coliform test procedure which is described herewith. Conduct the exercise following the steps enumerated herewith.

1. Label the lactose broth tubes and nutrient agar slants with the source of water sample.

2. Inoculate the lactose broth tubes and nutrient agar slants with the isolated coliform colonies on the EMB/Endo agar plates from each of the experimental water samples.

3. Incubate the inoculated broth tubes and slants at 37°C for 24 hours.

4. Examine lactose broth fermentation tubes for production of acid and gas and nutrient slants for gram reaction and cell morphology.

5. Production of acid and gas in inoculated lactose broth and presence of gram negative rods in nutrient slants indicate a positive complete test and confirms the presence of coliforms in water sample.


Related Discussions:- Explain procedure for performing the completed coliform test

Ecology, Which of the following are likely to help conserve soil and preven...

Which of the following are likely to help conserve soil and prevent erosion?

What is an oligopeptide, Q. What is an oligopeptide? How is it differing fr...

Q. What is an oligopeptide? How is it differing from a polypeptide? Peptide is the molecule formed by the union of amino acids through the peptide bond. Oligopeptide is a pepti

Glucose, Glucose is a six-carbon single sugar; It is the most common energ...

Glucose is a six-carbon single sugar; It is the most common energy source.

Workplace exposure limits, Question 1: What are the heat-induced disord...

Question 1: What are the heat-induced disorders that may result from excessive exposure to a hot working environment? How can we alleviate such health problems? Question 2:

Give the details of colour vision, Give the Details of Colour Vision Th...

Give the Details of Colour Vision The genes for colour sense are located on the X chromosome (recessive inheritance) and on chromosomes 3 and 7. Changes in the cone pigments as

What are the causes of constipation, Q. What are the Causes of constipation...

Q. What are the Causes of constipation? The most common causes of constipation are poor elimination habits, a lack of fibre in the diet, insufficient fluid intake, lack of exer

Compare the time-course to the rising phase, Assume you are injecting a sub...

Assume you are injecting a sub threshold depolarizing current step into a neuron. Draw the corresponding membrane voltage response; be sure to indicate the shape of the voltage cha

Evidence in favour and against of mutation theory, EVIDENC E IN FAVOUR OF ...

EVIDENC E IN FAVOUR OF THE MUTATION THOERY - Mutation theory can explain both progressive and retrogressive evolution and the occurrence of both changed and unchanged forms.

Microscopic examination of sputum, Question 1 Discuss briefly the collecti...

Question 1 Discuss briefly the collection and examination of pericardial fluid Question 2 Discuss the following Collection of CSF Physical examination of CSF

What is the role of heart in human body, What is the role of heart in human...

What is the role of heart in human body? The heart is the main pump that circulates the blood and fluid within the body. The heart, beats at 72 times per minute, and pumps abou

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd