Explain procedure for isolation of pure culture, Biology

Assignment Help:

Explain Procedure for Isolation of Pure Culture?

Now carry out the exercise following the steps enumerated herewith.

1. Label the name of the organism on the bottom of the petri plate.

2. Sterilize the inoculating loop by holding it with right hand in flame till it red hot.

3. Hold the culture tube in left hand and remove the cotton plug with the help of little finger of right hand.

4. Sterilize the mouth of culture tube immediately in flame and take culture (loopful if the culture is liquid broth or small amount if the culture is solid medium) with the sterilized inoculating loop. Replace the cotton plug and keep the tube in test tube rack.

5. Transfer the culture to the labeled petri plate by streaking. For the same, hold the plate in the left hand at an angle of 60° C and open the lid (Petri plate cover) with the help of the thumb.

6. Place the culture at one end of the plate and spread it slightly in a rounded manner. It is the primary inoculum. (Look at area 1 in the margin Figure).

7. Flame the loop and cool it. Then streak the inoculums at an angle to the primary inoculum by making 5-6 parallel lines. 1-2 lines should pass through the primary inoculum (as shown in area 2 in margin Figure). The flaming of the loop results in desired dilution and fewer and fewer organisms are streaked in each successive area leading to final separation of the microbial cells.

8. Reflame and cool the loop. Turn the plate at right angle. Drag the inoculum in several parallel lines across the agar surface (as shown in area 3 of margin Figure) by touching the inoculum in area 2 only once or twice.

9. Again rotate the Petri plate at 90°C angle. After flaming and cooling the inoculating loop, touch the inoculum in area 3 once and streak the inoculum several times in parallel lines across the agar. Look at the margin Figure the area marked 4.

10. Without reflaming the loop again turn the Petri plate at an angle and drag the culture from previous streak series (area 4) across area 5 in a similar manner by making parallel lines.

11. Replace the lid on Petri plate and incubate the culture for 24 hours (overnight) at 37°C. Observe the plates for isolated colonies. Margin Figure shows the plate after incubation.

12. Pick and restreak the isolated colony on another nutrient agar plate in a similar manner to get the pure culture.

 


Related Discussions:- Explain procedure for isolation of pure culture

Why are vaccines made of the own disease agent, Why are vaccines made of th...

Why are vaccines made of the own disease agent or of fragments of it? The goal of vaccines is to artificially persuade a specific primary immune response (and the consequent fo

Excretion, living organisms and their excretory products

living organisms and their excretory products

Nutrition in animals, composition,sources,importance,types,requirements for...

composition,sources,importance,types,requirements for each food substances

Eyeball - sclerotic, SCLEROTI C - White of eye. Outer thick, made u...

SCLEROTI C - White of eye. Outer thick, made up of fibres connective tissue. Protective layer. Give definite shape. Its anterior 1/6 part is thin, transparent known as

Define nutritional management of celiac disease, Define Nutritional Managem...

Define Nutritional Management of Celiac Disease? A gluten-free diet is the major therapy for celiac disease patients, particularly children, and may be needed for lifelong adhe

Illustrate dilated cardiomyopathy, Q. Illustrate Dilated cardiomyopathy? ...

Q. Illustrate Dilated cardiomyopathy? It is a disease of unknown etiology, affecting myocardium. Its diagnosis is established by presence of left ventricular dilatation and sys

To test the gas given off when seeds germinate, To test the gas given off w...

To test the gas given off when seeds germinate Place some mustard seeds in a flask with some damp cotton wool, in the apparatus shown in the diagram, and permit them to germina

Explain the complexity level of the nervous system, Q Is the tubular-dorsal...

Q Is the tubular-dorsal nervous system of chordates associated to radial or lateral symmetry? How does that explain the complexity level of the nervous system reached by the verteb

Transmission electron microscopy, Transmission Electron Microscopy The so...

Transmission Electron Microscopy The source of illumination in a TEM is a tungsten filament that emits electrons. A vacuum is created to prevent the collision of electrons with a

What are the typical features of mammals, What are the typical features of ...

What are the typical features of mammals? The typical features of mammals are: body (more or less) covered with hair; presence of the diaphragm muscle (that divides the thorax

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd