Explain procedure for capsule staining in a culture, Biology

Assignment Help:

Explain Procedure for Capsule Staining in a Culture?

We have learnt that Capsule staining can be undertaken either by negative staining or Anthony method. Now carry out the exercise by following the steps enumerated herewith.

For Negative Staining

1. Label the clean non-greasy slide.

2. Put a drop of nigrosin/India Ink dye at one end of the slide and mix it evenly with a loopful culture of given bacterial sample.

3. Spread the mixture over a slide as a thin film with the help of another slide held at 30o angle as illustrated in the figure in margin and pushed away to the other end of the slide.

4. Air dry the smear.

5. Stain the smear with Crystal violet/Safranin for 1 minute and wash gently with water.

6. Finally press the slide with the help of a filter paper and observe under oil-immersion objective of the microscope.

 For Anthony Staining Method

1. Take the clean non-greasy slide and prepare a bacterial smear.

2. Air dry the smear and flood the slide with crystal violet for 1-2 minutes.

3. Wash the slide with 20% copper sulphate and gently blot dry it.

4. Observe under oil immersion.


Related Discussions:- Explain procedure for capsule staining in a culture

Ecosystem degradation and wildlife, Ecosystem Degradation and Wildlife ...

Ecosystem Degradation and Wildlife Man is an animal with ecological requirements just like any other animal. It constantly needs a source of energy and.minera1 nutrients for i

Explain the lectins or haemagglutinins, Explain the Lectins or Haemagglutin...

Explain the Lectins or Haemagglutinins? These are sugar binding proteins, having the ability to bind and agglutinate red blood cells (RBCs). You will find that they occur in mo

Define repletion studies for studying nutrient requirement, Explain the Dep...

Explain the Depletion and Repletion Studies for studying nutrient requirement? This is an experimental procedure in which volunteer subjects are kept on a diet devoid of a part

Does the fish heart pump venous or arterial blood, Does the fish heart pump...

Does the fish heart pump venous or arterial blood? The venous blood coming from the tissues enters the atrium and passes to the ventricle that then pumps the blood towards the

State the term - myasthenia gravis, Myasthenia Gravis Myasthenia gravis...

Myasthenia Gravis Myasthenia gravis (severe muscle weakness) is characterised by muscular fatigue in the wake of very little exercise. It may be apparent after a short period o

Describe phases in the care process, There are 5  distinct yet constantly i...

There are 5  distinct yet constantly interacting phases in the care process. Assessment : A broad base of relative information about the patient's nutritional status,  food ha

Why did scientists want to map the human genome, Why did scientists want to...

Why did scientists want to map the human genome? They wanted to learn the location of all the significant genes in the genome in order to learn how the genome is organized, ho

What are examples of the ecological and economic, What are examples of the ...

What are examples of the ecological and economic importance of molluscs? Molluscs are significant players in several food chains in ecosystems. Many marine molluscs are part of

Vitamins, Vitamins Animals cannot sustain a healthy life if they are ...

Vitamins Animals cannot sustain a healthy life if they are fed on a diet having only carbohydrates, fats and proteins. They also require vitamins in small quantities in the r

Explain about blood pressure, Explain about Blood pressure Blood press...

Explain about Blood pressure Blood pressure is explained as the force exerted by the blood against the walls of the arteries. Blood pressure (BP) can be described as the fo

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd