Explain Precautions for Morphological Study of Fungi?

1. Use sterile forcep and needles for slide culture technique.

2. Aseptic conditions should be maintained.

 3. Carefully remove the coverslip and agar block for semi-permanent mount so that morphologic characteristics of the fungus won't disturb.

4. Entry of air bubbles should be avoided when placing the cover-slip over a drop of stain.

5. Use sufficient amount of stain so that it completely covers the 18 mm cover-slip.

6. Remoisten the filter paper with sterile water when it becomes dry.