Explain Precautions for Isolation of Pure Culture?

1. Plating of the medium should be done 24 hours before performing the streaking.

There should not be any moisture on the plate.

2. Petri plate lid should not be lifted completely.

3. Labeling of the plate should be done at the bottom of the plate.

4. Flaming and reflaming of the loop should be done to get the desired separation of the organism.

5. Before using the inoculating loop, cooling should be done by touching the agar surface away from the streaks, otherwise the organisms would be killed.

6. Maintain aseptic conditions.

7. Avoid pressing the loop too firmly against the agar surface as this will damage it.

8. Never let the loop to touch any of the previously streaked ones.