Explain precautions for isolation of pure culture, Biology

Assignment Help:

Explain Precautions for Isolation of Pure Culture?

1. Plating of the medium should be done 24 hours before performing the streaking.

There should not be any moisture on the plate.

2. Petri plate lid should not be lifted completely.

3. Labeling of the plate should be done at the bottom of the plate.

4. Flaming and reflaming of the loop should be done to get the desired separation of the organism.

5. Before using the inoculating loop, cooling should be done by touching the agar surface away from the streaks, otherwise the organisms would be killed.

6. Maintain aseptic conditions.

7. Avoid pressing the loop too firmly against the agar surface as this will damage it.

8. Never let the loop to touch any of the previously streaked ones.

 


Related Discussions:- Explain precautions for isolation of pure culture

Show characteristics of food products, Q. Show characteristics of food prod...

Q. Show characteristics of food products? These sensory characteristics of food products. Food quality, as such, can be divided into three main categories i.e. i. Appeara

What are the main prophylactic measures against malaria, Q. What are the ma...

Q. What are the main prophylactic measures against malaria? The major preventive measures against malaria are the elimination of the treatment of infected people, vector mosqui

Guidelines for diabetic patients for healthy coping, Q. Guidelines for Diab...

Q. Guidelines for Diabetic Patients for Healthy Coping? Generally, a diabetic patient adapts to day to day stress effectively, as they accept their disease condition with the s

What is the indolacetic acid (iaa), What is the indolacetic acid (IAA)? ...

What is the indolacetic acid (IAA)? IAA or Indolacetic acid (indolyl-3-acetic acid) is the major natural auxin made by plants. It promotes plant growth and cellular differentia

Gorgonids, Gorgonids yield chemicals like prostaglandins, medically useful ...

Gorgonids yield chemicals like prostaglandins, medically useful for birth control, prevention of peptic ulcers, treatment of asthma, regulation of blood pressure, etc. Sponges are

Class of mollusca - aplacophora, Class of Mollusca - Aplacophora Worm...

Class of Mollusca - Aplacophora Worm-like, no shell, head or excretory organs mantle with chitinous cuticle or scales or spicules mantle cavity posterior. Aplacophorans are a

What is the diffusion, Q What is the diffusion? Diffusion is the spread...

Q What is the diffusion? Diffusion is the spreading of substance molecules from a region where the substance is further more concentrated to another region where it is less con

What is hipot test ?, Hipot is an abbreviation for high potential. Usually,...

Hipot is an abbreviation for high potential. Usually, Hipot is a term given to a class of electrical safety testing instruments used to confirm electrical insulation in finished ap

What are the amylase inhibitors, What are the Amylase Inhibitors? Inhib...

What are the Amylase Inhibitors? Inhibitors of the enzyme a-amylase are found in wheat, rye, beans, mango, legumes, potatoes, sorghum (jowar) and oats. Most amylase inhibitors

Carbohydrates, Carbohydrate - contains Carbon , Hydrogen , and Oxygen ...

Carbohydrate - contains Carbon , Hydrogen , and Oxygen with a general formula of C x (H 2 O) y Simple sugars à monosaccharides Oligosaccharide à sugars containing

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd