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Explain Precautions for Gram Staining of Bacterial Cultures?
1. Heat fix the smear, otherwise cells would be washed off.
2. Fresh 24 hrs old culture should be used. Gram variability can be seen in old culture. Since with age gram Positives lose their ability to retain primary stain, in gram variability some cells appear purple and others appear red.
3. Decolourization is a critical step. Decolourization is done till the colour of the primary stain comes out. Over-decolourization can make gram positives appear gram negatives and under decolourization can cause gram negatives to appear as gram positives.
4. Slide should be thoroughly washed after every step to remove excess reagent under running water or water applied with an eye dropper.
detail notes on seed dormancy and seed germination
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Illustrate the Applications of vitamin B 6 The heating processes employed in the industry for the sterilization of milk based formulations will greatly reduce their vitamin B
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