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Explain Precautions for Gram Staining of Bacterial Cultures?
1. Heat fix the smear, otherwise cells would be washed off.
2. Fresh 24 hrs old culture should be used. Gram variability can be seen in old culture. Since with age gram Positives lose their ability to retain primary stain, in gram variability some cells appear purple and others appear red.
3. Decolourization is a critical step. Decolourization is done till the colour of the primary stain comes out. Over-decolourization can make gram positives appear gram negatives and under decolourization can cause gram negatives to appear as gram positives.
4. Slide should be thoroughly washed after every step to remove excess reagent under running water or water applied with an eye dropper.
The digitalis glycosides are the only orally active positive inotropic agents currently available. The positive inotropic occurs through inhibition of the enzyme Na + -K + -ATPase
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a nutrient agar plate labelled 10^(-5)ml had 154 colonies after incubated. what is the cell density in the original sample
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How many biochemicals would be detected in the stomach contents of an omnivorous dinosaur?
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Describe the factors that affect cardiac output in a female athlete who is speed skating toward the finish line in an Olympic race
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