Explain precautions for capsule staining in a culture, Biology

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Explain Precautions for capsule staining in a culture?

1. Never heat fix the smear. This is because by heating shrinkage can occur which may create a clear zone around the cell. It is an artifact and may be mistaken for a capsule.

2. Washing off the smear with water should be avoided because capsule may be removed with vigorous washing.

 


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