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Oxygen concentration
When oxygen is abundant, the rate of oxidation is independent of oxygen concentration, but at very low oxygen concentration, the rate is approximately proportional to oxygen concentration. However, the effect of oxygen concentration on rate is also influenced by other factors, such as temperature and surface area.
Isolation and identification of the etiological agent: Isolation of the etiological agent is attempted in chicken embryos, cell cultures, laboratory animals, culture medi
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