Explain methods of protein estimation, Biology

Assignment Help:

Explain Methods of Protein Estimation?

Several methods for protein estimation are available. These methods are based on several analytical characteristics of the protein. Laboratory estimation of protein is based on any of the following principles:

i) Nitrogen estimation based on the principle of Kjeldahl's method,

ii) Estimation of tyrosine in protein (as in Lowry's method) or both phenyl alanine and tyrosine in protein (Folin's method).

iii) Biuret method - most commonly used method in clinical practice and teaching laboratories.

We have studied some of the tests in qualitative methods and the principle is the same. The Biuret method for example can be used for both qualitative and quantitative estimation of proteins. Let us learn about these methods in details. We shall, however, focus on the kjeldahl's method and the biuret method here in this section.


Related Discussions:- Explain methods of protein estimation

Ph of food in pectic ulcer, Q. PH of food in pectic ulcer? PH of food: ...

Q. PH of food in pectic ulcer? PH of food: It has. a little therapeutic importance except for patients with lesions in the mouth or oesophagus. Most foods are considerably les

What are the trends of the gametophyte, What are the trends of the gametoph...

What are the trends of the gametophyte in the evolution of plants? A tendency of the gametophyte evolution in plants has been towards the formation of gametes that are independ

What is S shaped incision, Q. What is S shaped incision? The S - shaped...

Q. What is S shaped incision? The S - shaped incision is indicated where a papilla needs to be developed and was first described by Palacci. This type of incision is essentiall

Explain zipping of the apical canal, Explain Zipping of the Apical Canal ...

Explain Zipping of the Apical Canal a. If the preparation is continued with progressively larger and stiffer file lead to : zipping perforation or tear apical foramen.

Health conditions, 2. Mr Konarski, 73 years old, lives in a high-level care...

2. Mr Konarski, 73 years old, lives in a high-level care facility. He has dementia of the Alzheimer''s type, osteoarthritis in both knees and ankles, and hypertension, which is con

Explain the appearance of the pupil, Explain the Appearance of the Pupil ...

Explain the Appearance of the Pupil The pupil should appear round and regular. The normal diameter  is  2-4 mm. On stimulation by  light,  the pupil  constricts, so as  to redu

What are the risk factors for development of food allergy, What are the ris...

What are the risk factors for the development of food allergy? Having gone through the discussion above, can you identify at least one important factor leading to food allergy?

Hypomagnesaemic tetany of calves, Hypomagnesaemic tetany of calves You...

Hypomagnesaemic tetany of calves Young calves in the age group of 2-8 weeks and fed whole milk or milk replacer are more likely to develop hypomagnesaemia due to inadequate ma

Endodermis , Endodermis is the layer of cells surrounding the vascular cyl...

Endodermis is the layer of cells surrounding the vascular cylinder of plants.

Sucrose is non sugar but maltose and lactose are not. why?, Sucrose is a no...

Sucrose is a non-reducing Sugar while Maltose and Lactose are reducing due to the presence of free Aldehydic Functional Group in them. In SUgar the Aldehydic Group of both Glucose

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd