Explain methods of protein estimation, Biology

Assignment Help:

Explain Methods of Protein Estimation?

Several methods for protein estimation are available. These methods are based on several analytical characteristics of the protein. Laboratory estimation of protein is based on any of the following principles:

i) Nitrogen estimation based on the principle of Kjeldahl's method,

ii) Estimation of tyrosine in protein (as in Lowry's method) or both phenyl alanine and tyrosine in protein (Folin's method).

iii) Biuret method - most commonly used method in clinical practice and teaching laboratories.

We have studied some of the tests in qualitative methods and the principle is the same. The Biuret method for example can be used for both qualitative and quantitative estimation of proteins. Let us learn about these methods in details. We shall, however, focus on the kjeldahl's method and the biuret method here in this section.


Related Discussions:- Explain methods of protein estimation

What are pioneer species, What are pioneer species? What is the role of the...

What are pioneer species? What is the role of the pioneer species? Pioneer species are those first species that colonize places where before there were no living beings, like,

Explain ribavirin, Ribavirin (Copegus, Rebetol, and others)  Combinatio...

Ribavirin (Copegus, Rebetol, and others)  Combination treatment of HCV with peginterferon alfa and oral ribavirin has produced higher sustained response rates than peginterfero

Intracellular digestion, Intracellular Digestion We all know that unic...

Intracellular Digestion We all know that unicellular organisms do not have a separate alimentary canal system. All the functions of life are carried out inside a single cell.

What is a terrestrial organism, What is a terrestrial organism? 'Terra'...

What is a terrestrial organism? 'Terra' is the Latin word for earth. Thus, an animal that lives on the surface of the earth is known as terrestrial. This is the similar root wo

Define factors that affect the requirement of protein, Define Factors that ...

Define Factors that affect the requirement of Protein? Protein requirement is greatly influenced by many factors such as age, environmental temperature, energy intake, gender,

Into which two groups can the nitrogen-containing bases, Into which two gro...

Into which two groups can the nitrogen-containing bases that form DNA and RNA be classified? What is the criterion used in that classification? The nitrogen-containing bases th

Explain about the dna viruses - carcinogenic, Explain about the DNA viruses...

Explain about the DNA viruses - Carcinogenic? The three DNA viruses found to cause human cancers are EBV, HBV and HPV. What are these? Let us find out. Epstein-Barr viru

What is clinical deficiency presented by hemophilic people, What is the cli...

What is the clinical deficiency presented by hemophilic people? What is the genetic cause of that deficiency? The Hemophilia is a disease characterized by impaired blood clotti

Explain about enzymes, Explain about enzymes All enzymes are proteins. ...

Explain about enzymes All enzymes are proteins. Many enzyme  proteins lack catalytic activity in the absence of a non-protein component, called a 'co-factor'. In this case, the

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd