Explain methods of protein estimation, Biology

Assignment Help:

Explain Methods of Protein Estimation?

Several methods for protein estimation are available. These methods are based on several analytical characteristics of the protein. Laboratory estimation of protein is based on any of the following principles:

i) Nitrogen estimation based on the principle of Kjeldahl's method,

ii) Estimation of tyrosine in protein (as in Lowry's method) or both phenyl alanine and tyrosine in protein (Folin's method).

iii) Biuret method - most commonly used method in clinical practice and teaching laboratories.

We have studied some of the tests in qualitative methods and the principle is the same. The Biuret method for example can be used for both qualitative and quantitative estimation of proteins. Let us learn about these methods in details. We shall, however, focus on the kjeldahl's method and the biuret method here in this section.


Related Discussions:- Explain methods of protein estimation

List the four basic points of food exchange, List the four basic points to ...

List the four basic points to be kept in mind when advising food exchange. To develop the food exchange list points to keep in mind are: a) Group similar foods in one group.

Explain what is correction of hypovolemia, Explain what is Correction of Hy...

Explain what is Correction of Hypovolemia ? Intravenous fluids should be given to correct hypovolemia and optimize preload to the heart. This is particularly important in sept

How does the excretory system of nematodes work, Q How does the excretory s...

Q How does the excretory system of nematodes work? The metabolic residuals of nematodes are together by two longitudinal lateral excretory channels that open in one single excr

Explain zoe-cements - materials for root end filling, Explain ZOE-Cements -...

Explain ZOE-Cements - materials for Root End Filling a. Two  most widely accepted are: IRM and Super EBA (S-EBA) b. Both exhibit similar and favorable  c. Clinically and

Define surgery, Define Surgery? Surgery ! Does the word itself not crea...

Define Surgery? Surgery ! Does the word itself not create a feeling of anxiety and bring our thoughts towards a debilitating state of health which would be accompanied by pain,

The social environment - stress of hospitalization, The Social Environment ...

The Social Environment   When the child is admitted to the hospital his social environment is totally changed and all health team members are strangers for him. It is your resp

What is respiratory system explain their functionality , What is Respirator...

What is Respiratory System explain their functionality ? The respiratory system is responsible for the transport of oxygen and carbon dioxide from outside the body to and from

Crisis due to deforestation, Crisis due to Deforestation Due to conti...

Crisis due to Deforestation Due to continuing deforestation we are faced with a major ecological and socio-economic crisis. To reverse this trend of deforestation a National

What is concentration gradient, What is Concentration Gradient? The gra...

What is Concentration Gradient? The graduated dissimilarity in concentration of a solute per unit - distance by a solution.

Report writing, tell me how to write a report in molecular biology?

tell me how to write a report in molecular biology?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd