Explain methods of protein estimation, Biology

Assignment Help:

Explain Methods of Protein Estimation?

Several methods for protein estimation are available. These methods are based on several analytical characteristics of the protein. Laboratory estimation of protein is based on any of the following principles:

i) Nitrogen estimation based on the principle of Kjeldahl's method,

ii) Estimation of tyrosine in protein (as in Lowry's method) or both phenyl alanine and tyrosine in protein (Folin's method).

iii) Biuret method - most commonly used method in clinical practice and teaching laboratories.

We have studied some of the tests in qualitative methods and the principle is the same. The Biuret method for example can be used for both qualitative and quantitative estimation of proteins. Let us learn about these methods in details. We shall, however, focus on the kjeldahl's method and the biuret method here in this section.


Related Discussions:- Explain methods of protein estimation

Spermiogenesis - spermatogenesis, Spermiogenesis - Spermatogenesis At ...

Spermiogenesis - Spermatogenesis At the end of the meiosis the spermatids appear as simple spherical cells with a centrally located nucleus. Their differentiation into sperm r

Explain flucytosine, Explain Flucytosine Flucytosine (Ancobon) - Potent...

Explain Flucytosine Flucytosine (Ancobon) - Potentially lethal, dose-related bone mar- row toxicity, colitis and rapid development of resistance when used alone limit the use o

Zoology, characteristics of class mamalias

characteristics of class mamalias

Explain lateral meristem, Which one of the following is not a lateral meris...

Which one of the following is not a lateral meristem? 1. Intrafascicular cambium 2. Interfascicular cambium 3. Phellogen 4. Intercalary meristem   Intercalary m

How body fat can be measured, How body fat can be measured? The convent...

How body fat can be measured? The conventional golden method of measuring BF% is by underwater weighing. Difference of weight in air and in water gives density, from which the

Explain disease jet lag, Jet Lag   Disturbance of body and environmental...

Jet Lag   Disturbance of body and environmental rhythms resulting from a rapid change in time zones gives rise to jet lag, which is characterized by insomnia, decreased quality

Pain - criteria for endosteal implants, Q. Pain - criteria for endosteal im...

Q. Pain - criteria for endosteal implants? Pain and tenderness are subjective criteria and depend on patient's  interpretation of the degree of discomfort. Pain is defined as u

Explain the taxonomic concepts, Q. Explain the taxonomic concepts? The ...

Q. Explain the taxonomic concepts? The history of classification is an exciting aspect of plant taxonomy. The discovery of the use of plants for food and later as medicine bega

Which type of ecological interaction is competition, What is competition? W...

What is competition? Which type of ecological interaction is competition? Competition is the ecological interaction in which the individuals explore the similar ecological nich

How does the study of fossils strengthen theory of evolution, How does the ...

How does the study of fossils strengthen the theory of evolution? The study of fossils reveals extinct and ancient species having many similar structures to others of the prese

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd