Explain methods of protein estimation, Biology

Assignment Help:

Explain Methods of Protein Estimation?

Several methods for protein estimation are available. These methods are based on several analytical characteristics of the protein. Laboratory estimation of protein is based on any of the following principles:

i) Nitrogen estimation based on the principle of Kjeldahl's method,

ii) Estimation of tyrosine in protein (as in Lowry's method) or both phenyl alanine and tyrosine in protein (Folin's method).

iii) Biuret method - most commonly used method in clinical practice and teaching laboratories.

We have studied some of the tests in qualitative methods and the principle is the same. The Biuret method for example can be used for both qualitative and quantitative estimation of proteins. Let us learn about these methods in details. We shall, however, focus on the kjeldahl's method and the biuret method here in this section.


Related Discussions:- Explain methods of protein estimation

Carpentier-technique of operation, Carpentier: Edwards or similar annul...

Carpentier: Edwards or similar annuloplasty ring is then sutured into place by interrupted sutures to support the repair and reduce the annulus to a measured amount. The ring m

Effects of long term malnutrition, Effects of long term malnutrition ...

Effects of long term malnutrition The effects of long term malnutrition are more severe in children. Children do not have sufficient amounts of carbohydrates and fats in

What is the photoperiod, What is the photoperiod? The Photoperiod is th...

What is the photoperiod? The Photoperiod is the daily time period of light exposure of a living being and the photoperiod may differ according to the period of the year.

Solid or liquid at room temperature, do fats with one or more 'kinky' tail ...

do fats with one or more 'kinky' tail fatty acids tend to be solid or liquid at room temperature? These are found in triglycerides forming what? Solid fats or oils? Is it opposite

What do protozoans eat, What do protozoans "eat"? Do they move in search fo...

What do protozoans "eat"? Do they move in search for food? Protozoans are heterotroph beings, i.e., they do not make their own food and therefore they require to search for it

How do cells get energy for their functioning, Q How do cells get energy fo...

Q How do cells get energy for their functioning? Cells get energy for their metabolic reactions from the breaking of organic molecules with high energetic content. This energy

Molecular mechanism of hormone action, MOLECULAR MECHANISM OF HORMONE ACTIO...

MOLECULAR MECHANISM OF HORMONE ACTION - Once a hormone enters the bloodstream it can reach almost any cell in the body. However, each hormone affects only certain kinds of c

.phylum pisces, wt is an air bladder and its function wt is catadromous and...

wt is an air bladder and its function wt is catadromous and anadromus migration

Are body planes important for identifying structure, Q. Are body planes imp...

Q. Are body planes important for identifying anatomical structure? In order to observe and study structural arrangement of the internal organs, body may be divided and sectione

What is fixism, What is fixism? Fixism is the theory about the diversit...

What is fixism? Fixism is the theory about the diversity of life on earth that affirms that the current existent species were identical to species of the past and came out alre

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd