Explain methods of protein estimation, Biology

Assignment Help:

Explain Methods of Protein Estimation?

Several methods for protein estimation are available. These methods are based on several analytical characteristics of the protein. Laboratory estimation of protein is based on any of the following principles:

i) Nitrogen estimation based on the principle of Kjeldahl's method,

ii) Estimation of tyrosine in protein (as in Lowry's method) or both phenyl alanine and tyrosine in protein (Folin's method).

iii) Biuret method - most commonly used method in clinical practice and teaching laboratories.

We have studied some of the tests in qualitative methods and the principle is the same. The Biuret method for example can be used for both qualitative and quantitative estimation of proteins. Let us learn about these methods in details. We shall, however, focus on the kjeldahl's method and the biuret method here in this section.


Related Discussions:- Explain methods of protein estimation

What is contact osteogenesis, Contact Osteogenesis In this the osteoge...

Contact Osteogenesis In this the osteogenic cells attach to the implant surface and the new (de novo) bone is formed on the implant surface first. The developing bone matrix c

Explain about derived proteins, Derived Proteins These are not naturall...

Derived Proteins These are not naturally occurring proteins and are obtained from simple proteins by the action of enzymes and chemical agents, heat, mechanical shaking, UV or

What do you mean by herbaria and museums, Q. What do you mean by Herbaria a...

Q. What do you mean by Herbaria and Museums? A herbarium is a collection of pressed and dried plants arranged according to some valid system of classification and available for

What devices used for oxygen therapy, What Devices Used for Oxygen Therapy?...

What Devices Used for Oxygen Therapy? Variable performance systems: With these devices, the FiO 2 would vary depending on the patient's breathing pattern. In general, rapid, s

Peripatric speciation, Peripatric Speciation Speciation by small populat...

Peripatric Speciation Speciation by small populations isolated on the periphery of the distribution of the parent population can be described as peripatric speciation. Small, pe

Camptotheca acuminata, This species is an ornamental tree in China, and has...

This species is an ornamental tree in China, and has yielded the clinically active agents topotecan, irinotecan, and 9 -aminocamptothecin, which are semisynthetically derived from

Explain adverse effects of amantadine, Adverse Effects of amantadine  ...

Adverse Effects of amantadine  Amantadine may cause anorexia, nausea, peripheral edema and, particularly in the elderly, minor CNS effects such as nervousness, anxiety, insomn

Berry - development of seed, Berry - Development of Seed In a berry mu...

Berry - Development of Seed In a berry much of the pericarp is fleshy or juicy. In tomato Lycopersicon esculentum even the placenta on which the seeds are borne is fleshy. The

Placenta - human development, Placenta - Human Development We had said...

Placenta - Human Development We had said previous in the unit that in the second week of development a primitive uteroplacental circulation is established. Early in this stage

Regulation of egg, difference between mosaic and regulation egg

difference between mosaic and regulation egg

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd