Explain methods of protein estimation, Biology

Assignment Help:

Explain Methods of Protein Estimation?

Several methods for protein estimation are available. These methods are based on several analytical characteristics of the protein. Laboratory estimation of protein is based on any of the following principles:

i) Nitrogen estimation based on the principle of Kjeldahl's method,

ii) Estimation of tyrosine in protein (as in Lowry's method) or both phenyl alanine and tyrosine in protein (Folin's method).

iii) Biuret method - most commonly used method in clinical practice and teaching laboratories.

We have studied some of the tests in qualitative methods and the principle is the same. The Biuret method for example can be used for both qualitative and quantitative estimation of proteins. Let us learn about these methods in details. We shall, however, focus on the kjeldahl's method and the biuret method here in this section.


Related Discussions:- Explain methods of protein estimation

Suberin, SUBERIN It is a lipid formed by esterification of phellonic ac...

SUBERIN It is a lipid formed by esterification of phellonic acid or its derivative with glycerol. Suberin occurs in the walls of cork cells and endodermal cells. It makes

Describe about the term - neuropsychological assessment, Describe about the...

Describe about the term - Neuropsychological assessment Neuropsychological assessment has its roots in neurology, the branch of medicine that focuses on the nervous system and

Heart, Septa prevent oxygenated and deoxygenated blood. Give reason

Septa prevent oxygenated and deoxygenated blood. Give reason

What are the forces that make water to flow, What are the forces that make ...

What are the forces that make water to flow within the xylem from the roots to the leaves? Water enters the roots because of the root pressure and a water column is maintained

Management of hypertension, Q. Management of hypertension? the manageme...

Q. Management of hypertension? the management of hypertension, angina pectoris and myocardial infarction. It must be clear to you that some aspects of dietary management may di

How are the transparency and functions of cornea evaluated, How are the tra...

How are the transparency and functions of cornea evaluated? Physiological roles of barrier function, refractive function (with interaction of tear film), response to wound and

What is obesity in biochemical or physiological risk, What is Obesity in bi...

What is Obesity in biochemical or physiological risk ? Obesity results from a complex interaction of genetic predisposition and environmental factors operating throughout an in

Vitamin - a, VITAMIN - A Discovered by Macollum. Also known as ...

VITAMIN - A Discovered by Macollum. Also known as Retinol / Antixeropthalmic vitamin / Auxerophthol. It is vitamin for normal vision. It forms retinal pigments rho

List various type of insulin, Q. List various type of insulin? Types of...

Q. List various type of insulin? Types of insulin: a) Rapid or Short acting b) Intermediate c) Combination d) Long acting

Why the peritoneal cavity differ from the normal state, How does the presen...

How does the presence of thick, cloudy, yellowish fluid in the peritoneal cavity differ from the normal state?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd