Explain methods of protein estimation, Biology

Assignment Help:

Explain Methods of Protein Estimation?

Several methods for protein estimation are available. These methods are based on several analytical characteristics of the protein. Laboratory estimation of protein is based on any of the following principles:

i) Nitrogen estimation based on the principle of Kjeldahl's method,

ii) Estimation of tyrosine in protein (as in Lowry's method) or both phenyl alanine and tyrosine in protein (Folin's method).

iii) Biuret method - most commonly used method in clinical practice and teaching laboratories.

We have studied some of the tests in qualitative methods and the principle is the same. The Biuret method for example can be used for both qualitative and quantitative estimation of proteins. Let us learn about these methods in details. We shall, however, focus on the kjeldahl's method and the biuret method here in this section.


Related Discussions:- Explain methods of protein estimation

Micro biology, explane the role of nitrogenase enzyme

explane the role of nitrogenase enzyme

Explain serum lipoprotiens, Explain Serum lipoprotiens Serum lipoprot...

Explain Serum lipoprotiens Serum lipoprotiens:- spherical  or  ellipsoidal  particles containing  proteins, cholesterol esters  and  triacylglycerols, encased  within  a mon

Bio-insecticides and bio-pesticides, A large number of plants are used for ...

A large number of plants are used for bio-pesticides and bio-fertilizers. Several plant species are used as insecticides against aphids and larvae of insect pests: for insects like

Two chromosomes in cell have identical length, Two chromosomes in a cell ha...

Two chromosomes in a cell have identical length and centromere position yet are not homologous, what is different about them.

Illustrate sulfur photosynthetic bacteria, Q. What is the molecule that don...

Q. What is the molecule that donates hydrogen for photosynthesis, in sulfur photosynthetic bacteria? In sulfur photosynthetic bacteria the substance that donates hydrogen is hy

Introduction to ecology, Ecology is a interdisciplinary branch of science t...

Ecology is a interdisciplinary branch of science that deals with inter relationship between living organism and their environment. The term ecology (from greek, oikos-meaning ho

Location of overgloves, Location of Overgloves - taped to the sides of ...

Location of Overgloves - taped to the sides of the mobile cabinets for procedures such as patient examination and charting during non-aerosol producing procedures - on the p

Do plants have tissue organization and specialized organs, Do plants have t...

Do plants have tissue organization and specialized organs? Plants have specialized organs (as reproductive organs, roots, limbs, leaves) and differentiated tissues (vascular t

State the principle of adaptation, State the Principle of Adaptation Th...

State the Principle of Adaptation The first principle is that the central goal of assessment is to promote the adaptation of the child, rather than simply to document the prese

Explain spray-dried milk powder, Q. Explain Spray-dried milk powder? Th...

Q. Explain Spray-dried milk powder? The milk which is concentrated by the process of spray drying contains about 40- 45% total solids. Do you know how the dried milk powder i

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd