Explain methods of protein estimation, Biology

Assignment Help:

Explain Methods of Protein Estimation?

Several methods for protein estimation are available. These methods are based on several analytical characteristics of the protein. Laboratory estimation of protein is based on any of the following principles:

i) Nitrogen estimation based on the principle of Kjeldahl's method,

ii) Estimation of tyrosine in protein (as in Lowry's method) or both phenyl alanine and tyrosine in protein (Folin's method).

iii) Biuret method - most commonly used method in clinical practice and teaching laboratories.

We have studied some of the tests in qualitative methods and the principle is the same. The Biuret method for example can be used for both qualitative and quantitative estimation of proteins. Let us learn about these methods in details. We shall, however, focus on the kjeldahl's method and the biuret method here in this section.


Related Discussions:- Explain methods of protein estimation

Explain pollination and its types, Q. What is the pollination? What are the...

Q. What is the pollination? What are the major forms of pollination? The procedure in which pollen grains (the male gametophytes of phanerogamic plants) reach the female gameto

Chances of clinical risks, A foreign DNA has to target a precise chromosoma...

A foreign DNA has to target a precise chromosomal site and integrate with the host DNA. Are the methods of gene therapy strong enough to produce desired expression without mistakes

Define about the pyranose and furanose ring structure, Define about the Pyr...

Define about the Pyranose and Furanose ring structure? Monosaccharides forming a five-sided ring or a five membered ring (4 C and 1 O), like ribose, are called furanoses. Those

What is counsellor, Q. What is Counsellor? Counsellor is someone who gi...

Q. What is Counsellor? Counsellor is someone who gives advice about the problem. A counselor's role is to help patients help themselves. In our context  a counsellor is  a comm

Determine the meaning of response cost, Determine the meaning of Response c...

Determine the meaning of Response cost This is a type of punishment in which the subject has to return back the token (positive reinforcement) earned earlier if the subject dis

Biotechnology in characterization of animal biodiversity, Use of biotechnol...

Use of biotechnology in characterization of animal biodiversity. Genetic uniqueness of populations is measured by the relative genetic distances of such populations from each o

Explain the endocrine gland and the exocrine gland, What is the difference ...

What is the difference between the endocrine gland and the exocrine gland? Endocrine gland is a gland whose secretions (known as hormones) are collected by the blood and reach

Why different types of seizures may occur, Why Different types of seizures ...

Why Different types of seizures may occur? Different types of seizures may occur: 1) Generalized or tonic-clonic seizures, where the entire brain curtex is involved and post

Control and informative function of organic molecules, Q. What are the few ...

Q. What are the few examples of the control and informative function of organic molecules? Based on genetic information, organic molecules control the entire work of the cell. D

Association of fibrinogen with cardiovascular risk factors, Q. Association ...

Q. Association of Fibrinogen With Cardiovascular Risk Factors? Ans. Fibrinogen, a large glycoprotein made mostly in the liver, is a clotting factor that activates thrombin

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd