Explain methods of protein estimation, Biology

Assignment Help:

Explain Methods of Protein Estimation?

Several methods for protein estimation are available. These methods are based on several analytical characteristics of the protein. Laboratory estimation of protein is based on any of the following principles:

i) Nitrogen estimation based on the principle of Kjeldahl's method,

ii) Estimation of tyrosine in protein (as in Lowry's method) or both phenyl alanine and tyrosine in protein (Folin's method).

iii) Biuret method - most commonly used method in clinical practice and teaching laboratories.

We have studied some of the tests in qualitative methods and the principle is the same. The Biuret method for example can be used for both qualitative and quantitative estimation of proteins. Let us learn about these methods in details. We shall, however, focus on the kjeldahl's method and the biuret method here in this section.


Related Discussions:- Explain methods of protein estimation

Oogenesis, in human and mammals how many cells result from oogenesis

in human and mammals how many cells result from oogenesis

Explain zipping of the apical canal, Explain Zipping of the Apical Canal ...

Explain Zipping of the Apical Canal a. If the preparation is continued with progressively larger and stiffer file lead to : zipping perforation or tear apical foramen.

Epithelial tissue, conclusion for epithelial tissue when finish experiment?...

conclusion for epithelial tissue when finish experiment?

Reduction in the birth rate, State three conditions in a society which coul...

State three conditions in a society which could contribute to a reduction in the birth rate. Birth rate might be decreased by (i) later marriages (as smaller families), (

Which pga as the first co2 fixation product, PGA as the first CO2 fixation ...

PGA as the first CO2 fixation product was discovered in photosynthesis of: 1. Bryophyte 2. Gymnosperm 3. Angiosperm 4. Alga Alga

Munch pressure flow model, Munch Pressure Flow Model Munch, a German p...

Munch Pressure Flow Model Munch, a German plant physiologist, proposed in 1930, a simple physical model which can be tested in the laboratory for the mechanism of phloem trans

Define hocm murmur characteristics in details, Define HOCM Murmur character...

Define HOCM Murmur characteristics in details? Characteristics: The murmur is harsh, crescendo-decrescendo audible along the left sternal border, radiates to the base of heart

Radiometer-radiation instrument, Radiometer Radiometer measures the flu...

Radiometer Radiometer measures the flux of energy of all wavelengths received on a single surface of the receiver. There are also instruments that can measure the difference be

Indirect uses of biodiversity, The cost of replacing these indirect uses of...

The cost of replacing these indirect uses of biodiversity, even if it were technically possible, would be so high as to render it impossible to duplicate. We also do not know how

Define objectives to know about methods of food processing, Define Objectiv...

Define Objectives to know about methods of food processing? After studying this unit, you will be able to: Enumerate the different methods of food processing Enlist

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd