Explain methods of protein estimation, Biology

Assignment Help:

Explain Methods of Protein Estimation?

Several methods for protein estimation are available. These methods are based on several analytical characteristics of the protein. Laboratory estimation of protein is based on any of the following principles:

i) Nitrogen estimation based on the principle of Kjeldahl's method,

ii) Estimation of tyrosine in protein (as in Lowry's method) or both phenyl alanine and tyrosine in protein (Folin's method).

iii) Biuret method - most commonly used method in clinical practice and teaching laboratories.

We have studied some of the tests in qualitative methods and the principle is the same. The Biuret method for example can be used for both qualitative and quantitative estimation of proteins. Let us learn about these methods in details. We shall, however, focus on the kjeldahl's method and the biuret method here in this section.


Related Discussions:- Explain methods of protein estimation

Multiplication phas - stages of spermatogenesis, Multiplication Phas - Stag...

Multiplication Phas - Stages of spermatogenesis The initial cells in the germ line arc known as primordial germ cells (PCC). The PGC, which arise at some distance from the pro

What is contact plate method - food microbiology, What is Contact Plate Met...

What is Contact Plate Method? Contact Plate (RODAC - Replicate Organism Direct Agar Contact) Method - Contact plates are special petri plates containing suitable agar media wit

Similarities between pisces and amphibians, Ask question #Minimuwhat are th...

Ask question #Minimuwhat are the similarities between pisces and amphibians

Can you define p-waves, Q. Can you define P-Waves? Changes in P-wave mo...

Q. Can you define P-Waves? Changes in P-wave morphology have been well described in resting tracings and are very useful in identifying right and left sided hemodynamic alterat

Foetal maceration and mummification, Foetal maceration and mummification ...

Foetal maceration and mummification Foetal mummification occurs when the foetus dies in the uterus in the absence of air and bacterial contamination and the cervix remains tig

Describe the factors that affect cardiac output, Describe the factors that ...

Describe the factors that affect cardiac output in a female athlete who is speed skating toward the finish line in an Olympic race

Explain whipping foam method, Explain Whipping foam method? Whipping is...

Explain Whipping foam method? Whipping is the most common method used as it forms bubbles by cutting the surface and introducing air into liquid.  Repeated action makes the

Synthesis of polyamine, Synthesis of Polyamine As the name of this cla...

Synthesis of Polyamine As the name of this class of compounds suggests, polyamines have several amino groups replacing hydrogen usually in alkyl chain e.g., putrescine is 1, 4

Risk during crown removal-endodontics principles, Risk during crown removal...

Risk during crown removal -Tooth inadvertently extracted using a crown\bridge remover . -Endodontic therapy was performed , and the tooth was replanted , a procedure known

Adenylate cyclase in the liver cell membrane, Adenylate cyclase  in  the ...

Adenylate cyclase  in  the liver cell membrane Glucagon activates adenylate cyclase  in  the liver cell membrane and thus turns on glycogenolysis and reduces glycogenesis. Insu

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd