Explain methods of protein estimation, Biology

Assignment Help:

Explain Methods of Protein Estimation?

Several methods for protein estimation are available. These methods are based on several analytical characteristics of the protein. Laboratory estimation of protein is based on any of the following principles:

i) Nitrogen estimation based on the principle of Kjeldahl's method,

ii) Estimation of tyrosine in protein (as in Lowry's method) or both phenyl alanine and tyrosine in protein (Folin's method).

iii) Biuret method - most commonly used method in clinical practice and teaching laboratories.

We have studied some of the tests in qualitative methods and the principle is the same. The Biuret method for example can be used for both qualitative and quantitative estimation of proteins. Let us learn about these methods in details. We shall, however, focus on the kjeldahl's method and the biuret method here in this section.


Related Discussions:- Explain methods of protein estimation

Define the high blood plasma lh levels and ovulation, Which of the followin...

Which of the following pairs of events in a human female occur at, or nearly at, the similar time during the menstrual cycle? A. High blood plasma LH levels and ovulation. B

Define reagents required and methodology for benedict test, Define reagents...

Define reagents required and methodology for Benedict Test? Reagents Required - Sugar solutions of glucose, fructose, galactose, maltose, lactiose, sucrose and starch.

Define historical development of food processing, Define Historical Develop...

Define Historical Development of Food Processing? Food processing began thousands of years ago to help people keep food through the lean seasons. Several methods of preserving

Explain about the plateau effect - energy balance, Explain about the platea...

Explain about the plateau effect - Energy Balance? You must have noticed that when people start following weight reducing diets, they lose weight rapidly in the beginning, then

Chain termination method, An incubation  combination  is set up having  the...

An incubation  combination  is set up having  the single-stranded  DNA template, DNA polymerase  I, the primer and all 4 deoxyribonucleoside triphosphates (dTTP, dGTP, dCTP, dATP),

What is the etiological agent, What is the etiological agent and the main m...

What is the etiological agent and the main manifestations of cholera? Cholera is a bacterial disease caused by the Vibrio cholerae. The disease is transmitted by fecal-oral rou

Elongation: what is translocation, A Complex  of  elongation  factor  EF-G ...

A Complex  of  elongation  factor  EF-G  (also known as  translocase)  and  GTP example for  EF-G/GTP binds  to the  ribosome.  There are three concerted movements  now happen coll

What is ecology?, What is Ecology? The Ecology is the field of Biology ...

What is Ecology? The Ecology is the field of Biology that studies the relationship between living beings and between living beings and the environment.

Examples of tissues and organs where mitosis is frequent, Q What are some e...

Q What are some examples of tissues and organs where mitosis is more frequent, less frequent or practically absent? Generally in vertebrates mitosis is more frequent in tissues

Aortic valve repair-types of surgery, Aortic Valve Repair :  ...

Aortic Valve Repair :  In, acquired AR associated with VSD and prolapse of a cusp, repair is often successful. VSD is closed and at the same time the prolapsed part o

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd