Explain methods of protein estimation, Biology

Assignment Help:

Explain Methods of Protein Estimation?

Several methods for protein estimation are available. These methods are based on several analytical characteristics of the protein. Laboratory estimation of protein is based on any of the following principles:

i) Nitrogen estimation based on the principle of Kjeldahl's method,

ii) Estimation of tyrosine in protein (as in Lowry's method) or both phenyl alanine and tyrosine in protein (Folin's method).

iii) Biuret method - most commonly used method in clinical practice and teaching laboratories.

We have studied some of the tests in qualitative methods and the principle is the same. The Biuret method for example can be used for both qualitative and quantitative estimation of proteins. Let us learn about these methods in details. We shall, however, focus on the kjeldahl's method and the biuret method here in this section.


Related Discussions:- Explain methods of protein estimation

Non migrainous vascular headaches, Non Migrainous Vascular Headaches Th...

Non Migrainous Vascular Headaches This headache associated with dilation of the cranial arteries and can be induced by a wide variety of diseases and conditions. The most commo

#sperm, #sperm of reptilia

#sperm of reptilia

Adaptations to high wind velocity, Adaptations to high wind velocity Th...

Adaptations to high wind velocity The mechanical force of the wind and the grinding action of sand, dust, snow and other materials driven by it cause the plants to adapt themse

History - assessment of patents with cardiovascular probems, History P...

History Past and present history of cardiovascular problems of patient & family. History of chest pain, shortness of breath, fatigue, abnormal skin colour, dizziness, vertigo

How is reproduction done in beings of the class reptilia, How is reproducti...

How is reproduction done in beings of the class Reptilia? These beings reproduce sexually by internal fecundation by means of copulation among male and female individuals. They

Extended point within pulp chamber-endodontics principles, Extended point w...

Extended point within pulp chamber: Gripping action: -    Steglitz forceps or pin pilers. -    Access cavity flooded with chloroform to dissolve the sealer. -    Grasp the

Interventricular septum, It corresponds to the anterior and inferior interv...

It corresponds to the anterior and inferior interventricular grooves. Its right side is convex and bulges to the right ventricle. The septum has a posterior border which separates

What is the function of lymph nodes, Explain function of lymph nodes? L...

Explain function of lymph nodes? Lymph nodes have white blood cells which ingest bacteria and prevent them from reaching the circulation.

Types of asexual reproduction, TYPES OF ASEXUAL REPRODUCTION ...

TYPES OF ASEXUAL REPRODUCTION 1 . Binar y fission - Amoeba 2. Multipl e fission -

Food-borne clostridium, Food-borne Clostridium Clostridium botulinum a...

Food-borne Clostridium Clostridium botulinum and Cl. perfringens, the two members of the group have been implicated in food-borne illnesses. C. botulinum causes classical food

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd