Explain methods of protein estimation, Biology

Assignment Help:

Explain Methods of Protein Estimation?

Several methods for protein estimation are available. These methods are based on several analytical characteristics of the protein. Laboratory estimation of protein is based on any of the following principles:

i) Nitrogen estimation based on the principle of Kjeldahl's method,

ii) Estimation of tyrosine in protein (as in Lowry's method) or both phenyl alanine and tyrosine in protein (Folin's method).

iii) Biuret method - most commonly used method in clinical practice and teaching laboratories.

We have studied some of the tests in qualitative methods and the principle is the same. The Biuret method for example can be used for both qualitative and quantitative estimation of proteins. Let us learn about these methods in details. We shall, however, focus on the kjeldahl's method and the biuret method here in this section.


Related Discussions:- Explain methods of protein estimation

Progressive senescence - senescence, Progressive senescence - Senescence ...

Progressive senescence - Senescence This is the third category of senescence. Here also the leaves are shed but it is gradual senescence of leaves up the stem. For example, th

Reviews - assurance engagements, Reviews - Assurance engagements The o...

Reviews - Assurance engagements The objective of financial statements of a review is to enable an auditor to state where, on the essential of procedures that do not give all t

Main parts into which a neuron can be divided, Q. What are the three main p...

Q. What are the three main parts into which a neuron can be divided? What are their respective functions? The three mains parts into which a neuron can be didactically divided

Explain the plaque assessment, Plaque Assessment Microbial films have b...

Plaque Assessment Microbial films have been known to form on inert biomaterial surfaces in an aqueous environment. Implants placed in the oral cavity are no exception and repre

Explain the process of absorption of fats, Explain the process of Absorptio...

Explain the process of Absorption of Fats? After digestion, only 25% of triglycerides are broken completely to glycerol and fatty acids. Major digestion product is 2-monoacylgl

Define pectin, Pectin The word pectin is derived from a Greek word whic...

Pectin The word pectin is derived from a Greek word which means to "congeal or solidify". Pectin is an acidic structural polysaccharide, found in fruit and vegetables and mainl

Tracheae - respiratory organs, Tracheae - Respiratory Organs Tracheal ...

Tracheae - Respiratory Organs Tracheal respiration is characteristic of insects. Onychophorans, diplopods, arachnids, and chilopods as well use tracheal system for respiration

Explain restricted fragment polymorphism, Q. Explain Restricted fragment po...

Q. Explain Restricted fragment polymorphism? This method makes use of a group of enzymes termed restriction enzymes which recognise particular sequence of DNA and act as "molec

Define blood pressure, Blood Pressure Blood pressure may be defined as ...

Blood Pressure Blood pressure may be defined as the force or pressure which the blood exerts on the walls of the artery. Blood pressure varies with the age of the individual. B

The principle of dna cloning, Assume an experimental target that is to make...

Assume an experimental target that is to make vast amounts of a particular DNA fragment in pure form from a combination of DNA fragments.  Whereas the DNA fragments can be introduc

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd