Explain methods of protein estimation, Biology

Assignment Help:

Explain Methods of Protein Estimation?

Several methods for protein estimation are available. These methods are based on several analytical characteristics of the protein. Laboratory estimation of protein is based on any of the following principles:

i) Nitrogen estimation based on the principle of Kjeldahl's method,

ii) Estimation of tyrosine in protein (as in Lowry's method) or both phenyl alanine and tyrosine in protein (Folin's method).

iii) Biuret method - most commonly used method in clinical practice and teaching laboratories.

We have studied some of the tests in qualitative methods and the principle is the same. The Biuret method for example can be used for both qualitative and quantitative estimation of proteins. Let us learn about these methods in details. We shall, however, focus on the kjeldahl's method and the biuret method here in this section.


Related Discussions:- Explain methods of protein estimation

The human abo blood type codominant alleles a and b, The human abo blood ty...

The human abo blood type codominant alleles a and b are dominant to O. A man with blood type B and women with blood type A could potentially have children with which of the followi

Excretory system, what is the excretory organ of a lizard?

what is the excretory organ of a lizard?

Clinical reasoning , Upon admission the DPU nurse notes: Fasting sta...

Upon admission the DPU nurse notes: Fasting status - fasted from 12 midnight, no morning medication taken. Consent - signed Pre anaesthetic check Test results

Leptospirosis, L e p t o s p i r o s i s It is zoonotic dis...

L e p t o s p i r o s i s It is zoonotic disease characterized by interstitial nephritis, haemolytic anaemia and abortions. E t iology : The disease i

Define the term - microfilaments and microtubules, Define the term - microf...

Define the term - microfilaments and microtubules The cytoskeleton is, in fact, termed as the "bone and muscle" of the eukaryotic cell, and is composed of a network, consisting

Cracking, Cracking is the process in which large molecules are broken into ...

Cracking is the process in which large molecules are broken into smaller molecules.                      C 10 H 22         C 5 H 12 + C 5 H 10                      C 8 H

Determine the chance of developing cancer, A typical chest x-ray exposes th...

A typical chest x-ray exposes the patient to a radiation dose of 0.01 rem. If a man receives 10 such x-rays in a lifetime, what is his chance of developing cancer as a result of th

What type of compound is major metabolic waste of porifera, What type of co...

What type of compound is the major metabolic waste of Porifera? Why is it important for the organism to get rid of this compound?

Show geniculate bodies of the thalamus, Q Show Geniculate Bodies of the tha...

Q Show Geniculate Bodies of the thalamus? THALAMUS - two large circular shaped masses at "roof" of 3rd brain ventricle. Contains many different nuclei which function as relay

Explain the meaning of protein deficiency, Explain the Protein Deficiency? ...

Explain the Protein Deficiency? One of the most common nutritional disorders in the world today is the deficiency of protein. Both adults and children are affected, as the popu

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd