Explain methods of protein estimation, Biology

Assignment Help:

Explain Methods of Protein Estimation?

Several methods for protein estimation are available. These methods are based on several analytical characteristics of the protein. Laboratory estimation of protein is based on any of the following principles:

i) Nitrogen estimation based on the principle of Kjeldahl's method,

ii) Estimation of tyrosine in protein (as in Lowry's method) or both phenyl alanine and tyrosine in protein (Folin's method).

iii) Biuret method - most commonly used method in clinical practice and teaching laboratories.

We have studied some of the tests in qualitative methods and the principle is the same. The Biuret method for example can be used for both qualitative and quantitative estimation of proteins. Let us learn about these methods in details. We shall, however, focus on the kjeldahl's method and the biuret method here in this section.


Related Discussions:- Explain methods of protein estimation

Illustrate nernst equilibrium potential for potassium ions, Illustrate Nern...

Illustrate Nernst equilibrium potential for potassium ions A complete motor neuron is removed from a frog and placed in normal physiological saline at 1 AM.  The neuron is hea

How different are gymnosperms from bryophytes, How different are gymnosperm...

How different are gymnosperms from bryophytes and pteridophytes? Gymnosperms are not cryptogamic as bryophytes and pteridophytes are. They are phanerogamic and so they form flo

What are uses of hydrocolloids for health, Uses of Hydrocolloidsfor health ...

Uses of Hydrocolloidsfor health Hydrocolloids, together with other dietary fibres are increasingly being seen as contributing to a number of health effects. Some of the hydroco

Does thermal inversion occur in the winter or in the summer, Q. Does therma...

Q. Does thermal inversion occur in the winter or in the summer? The Pollutant low altitude thermal inversion take place in the winter and in this period of the year the sun hea

Define cerebral hemisphere tissue, Q. Define cerebral hemisphere tissue? ...

Q. Define cerebral hemisphere tissue? CEREBRAL WHITE MATTER - The remaining cerebral hemisphere tissue is the deeper cerebral white matter (60% of cerebral volume) which includ

How many times acidic is a ph=4, How many times more acidic is a pH=4 from ...

How many times more acidic is a pH=4 from a pH=7 solution?

How respiratory pigments act, Q. How respiratory pigments act? Respirat...

Q. How respiratory pigments act? Respiratory pigments are oxygen-carrying molecules present in the blood. When the oxygen concentration is high for instance, in the pulmonary a

What is photoperiodism, What is photoperiodism? Photoperiodism is the ...

What is photoperiodism? Photoperiodism is the biological response shown by some living beings to their daily time of light exposure (photoperiod).

Principles of cardiopulmonary bypass mechanism (cpb), PRINCIPLES OF CARDIOP...

PRINCIPLES OF CARDIOPULMONARY BYPASS MECHANISM (CPB) What is CPB? It is a technique by which the mechanical function of the heart and respiratory function of the lungs

How vitamin e is directly related to vitamins a, How vitamin e is directly ...

How vitamin e is directly related to Vitamins A, D and K? A high intake of vitamin E interferes with the functions of other fat-soluble vitamins such as vitamin K absorpt

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd