Explain methods of protein estimation, Biology

Assignment Help:

Explain Methods of Protein Estimation?

Several methods for protein estimation are available. These methods are based on several analytical characteristics of the protein. Laboratory estimation of protein is based on any of the following principles:

i) Nitrogen estimation based on the principle of Kjeldahl's method,

ii) Estimation of tyrosine in protein (as in Lowry's method) or both phenyl alanine and tyrosine in protein (Folin's method).

iii) Biuret method - most commonly used method in clinical practice and teaching laboratories.

We have studied some of the tests in qualitative methods and the principle is the same. The Biuret method for example can be used for both qualitative and quantitative estimation of proteins. Let us learn about these methods in details. We shall, however, focus on the kjeldahl's method and the biuret method here in this section.


Related Discussions:- Explain methods of protein estimation

Science class, Im learning about difference cell structures in science. I w...

Im learning about difference cell structures in science. I was wondering if I might be able to get some information about the epithelial tissue, and the connective tissue. Thankyou

Define ovule, A vertical section of an ovule is shown below.  The correct p...

A vertical section of an ovule is shown below.  The correct ploidy levels of the four structures P, Q, R and S respectively are: a.  n, 2n, n, n b.  2n, n, n, n c.

What is the typical vegetation of the grasslands, What is the typical veget...

What is the typical vegetation of the grasslands? Grasslands are mostly formed of herbaceous (nonwoody) vegetation: grass, bushes and small trees. Biomes - Image Diversity:

Human population-historical overview, Human Population-Historical Overview ...

Human Population-Historical Overview Throughout history, the human population has been quite small. It has grown relatively slowly and even experienced occasional declines. Fi

Name the molecule that donates hydrogen for photosynthesis, In sulfur photo...

In sulfur photosynthetic bacteria what is the molecule that donates hydrogen for photosynthesis? In sulfur photosynthetic bacteria the substance that donates hydrogen is hydrog

Some common air pollutants: hydrocarbon, Hydrocarbon (HC): Hydrocarbons ...

Hydrocarbon (HC): Hydrocarbons such as methane, ethylene, acetylene is present in air. Most of these are low molecular weight gases and liquids at ordinary temperature. Sour

What are the three parts of a dna nucleotide, What are the three parts of a...

What are the three parts of a DNA nucleotide, and how are they connected to each other? The three types are a deoxyribose sugar, a phosphate group, and a nitrogenous base. The

Explain genital herpes, Explain Genital herpes Acyclovir  (Zovirax,  a...

Explain Genital herpes Acyclovir  (Zovirax,  and others), famciclovir (Famvir) or valacyclovir (Valtrex) taken orally for 7-10 days shortens the duration of pain, viral sheddi

Brief descriptions of how to test, If sugar is heavier than aspartame, then...

If sugar is heavier than aspartame, then the can of regular soda will sink. How will you test this hypothesis? Write brief descriptions of how you could test?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd