Explain methods of protein estimation, Biology

Assignment Help:

Explain Methods of Protein Estimation?

Several methods for protein estimation are available. These methods are based on several analytical characteristics of the protein. Laboratory estimation of protein is based on any of the following principles:

i) Nitrogen estimation based on the principle of Kjeldahl's method,

ii) Estimation of tyrosine in protein (as in Lowry's method) or both phenyl alanine and tyrosine in protein (Folin's method).

iii) Biuret method - most commonly used method in clinical practice and teaching laboratories.

We have studied some of the tests in qualitative methods and the principle is the same. The Biuret method for example can be used for both qualitative and quantitative estimation of proteins. Let us learn about these methods in details. We shall, however, focus on the kjeldahl's method and the biuret method here in this section.


Related Discussions:- Explain methods of protein estimation

Explain adsorption - colloidal particle, Adsorption - Colloidal particle ...

Adsorption - Colloidal particle Colloidal particles attract and hold to their surfaces the molecules of various gases, vapors and other matter with which they come into contact

Indications for surgery-pulmonary regurgitation, Indications for Surgery : ...

Indications for Surgery : Patients usually present with fatigue, dyspnoea and ventricular arrhythmias. If they have additional tricuspid regurgitation, pulmonary valve replacement

Explain the procedure for morphological study of fungi, Explain the Procedu...

Explain the Procedure for Morphological Study of Fungi? Now carry out the exercise, following the steps enumerated herewith: 1. Prepare a humid chamber by placing two-three

Use of alternate metabolic pathways, Use of Alternate Metabolic Pathways ...

Use of Alternate Metabolic Pathways Plants surviving under water-logged conditions offer the simplest example of such a response. The submerged parts of such plants use the an

Minerals and reproduction, Minerals and reproduction The mechanism of mine...

Minerals and reproduction The mechanism of mineral-reproduction interactions is not fully understood because  of the complexity of neuro-hormonal dialogue. Some minerals act direc

Sociability - qualitative characters, Sociability (Gregariousness) Soc...

Sociability (Gregariousness) Sociability refers to the nature of grouping of individual plants, that is, whether they grow singly, in patches, in colonies or evenly intermixed

Zoology, give an account of specific characters of phylum porifera

give an account of specific characters of phylum porifera

Evolution of man, diffrentiate between javaman and peckingman

diffrentiate between javaman and peckingman

Explain surlyn film, Q. Explain Surlyn film? Surlyn films: Surlyn (SU) ...

Q. Explain Surlyn film? Surlyn films: Surlyn (SU) is a versatile film which is sometimes favoured over polyethylene due to some unique properties such as excellent moisture b

Define abbe condenser of microscope, Define Abbe Condenser of Microscope? ...

Define Abbe Condenser of Microscope? It is present beneath the stage, as shown in Figure. It collects and focuses a cone of light on the slide. Its position can be adjusted ver

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd