Explain methodology required for osazone test, Biology

Assignment Help:

Explain Methodology Required for Osazone Test?

Take about 250-300 mg of phenylhydrazine mixture in the 3 clean labelled 15 ml dry test tubes. Add 5 ml of the glucose, fructose and galactose solution to each, separately.  Shake and mix the contents of each tube and heat in a boiling water bath (water in the water bath should be boiling vigorously when you put the tubes and should continue to do so till the tubes are removed) for 30- 45 minutes. Remove the tubes from the water bath and put them in a test tube rack. Allow the tubes to cool slowly (not under the tap).

Take  slides, label them as Glu, Gal, Fru, Mal, lac, Suc and with the help of a dropper take out a drop of the yellow precipitate like crystals from each tube and put it on the respective slide. Examine the crystals on each slide microscopically.


Related Discussions:- Explain methodology required for osazone test

Osmoregulation in non-chordate metazoans, Osmoregulation in Non-Chordate Me...

Osmoregulation in Non-Chordate Metazoans Previously you have studied that in protozoans, particularly fresh water protozoans; contractile vacuoles play a significant role in t

How auxins act helping the lateral growth of the stem, How auxins act helpi...

How auxins act helping the lateral (secondary) growth of the stem? The Auxins stimulate the formation of conductive vessels, phloem and xylem, promoting the thickening of the s

Impact of technological change on the cost of health service, Impact of Tec...

Impact of Technological Change on the Cost of Health Service Technological developments entail improvement in production/service frontiers either by providing cost benefit adv

Explain spray-drying method, Spray-drying method Spray-drying is the co...

Spray-drying method Spray-drying is the common drying method in the production of ISP. The primary physical form of ISP in commerce, is that of fine powders. Structured form

Things to do during a cyclone, (a)   For any individual :               ...

(a)   For any individual :                  ·          We should check house, doors and windows and have them repaired whenever necessary.                  ·          We shou

Heterotrophic nutrition, heterotrophic nutrition in any 5 type...

heterotrophic nutrition in any 5 types of organism

Ultrasonic removal of gutta percha, Ultrasonic removal of Gutta percha: ...

Ultrasonic removal of Gutta percha: -the piezoelestic ultrasonic system. - As the instruments is energized; producing heat to themosoften gutta-percha. - Float gutta-perc

Glucose-6-phosphate, Three molecules of ducose-6-phosphate  yield 3 molecul...

Three molecules of ducose-6-phosphate  yield 3 molecules of CO,  and 3 molecules of five carbon residues (pentose sugar). The latter are converted ultimately to  2 molecules  of  g

Blood salvage and bloodless open-heart surgery, Blood Salvage and Bloodless...

Blood Salvage and Bloodless Open-heart Surgery: At the time of cardio pulmonary bypass, cardiomony suckers suck blood from the chambers or the heart and pericardium back into the

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd