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Explain Isoelectric pH
Isoelectric pH: Many ionizable groups are present in a protein molecule. Depending on the pH of the medium, some of these groups act as proton donors and some others act as proton acceptors. Thus, proteins are amphoteric compounds. At a specific pH, the protein exists as a dipolar ion (one positive and one negative ion) or zwitterion. So, at this pH the net charge of the protein - becomes zero. This pH is known as isoelectricpHorpI of the protein. Having no net charge, protein does not move to either electrode in an electric field.
Enzyme inactivation The disappearance of activity of an enzyme (in vivo or in vitro due to presence of inhibitor molecules or inhibitory condition(changes in pH, temperature,
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The hospital Human Research Ethics Committee granted approval for the study protocol. Participants provided written informed consent. All participants were all over the age of 18 a
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