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Hydrolyases
These enzymes (code EC 3) catalyze the hydrolytic cleavage of C-O, C-N, C-C and some other bonds, including phosphoric anhydride bonds. Their trivial names are formed by adding the suffix 'ase ' to the substrate which they hydrolyze. Examples include protease, nuclease, phosphatase. A number of hydrolases acting on ester, glycosyl, peptide, amide or other bonds are known to catalyze not only the hydrolytic removal of a particular group from their substrates, but also the transfer of this group to a suitable acceptor molecule. Yet, they are not grouped as transferases, because the transfer of a specific group to water as the acceptor molecule is considered to be their main physiological function.
Transfer RNA molecules are notable for having generally nucleotides shown in the figure such as 1-methylguanosine (m1G), pseudouridine (Ψ), dihydrouridine (D), inosine (I) and
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