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Q. Explain Hydraulic Formula?
This relates to pressure gradient and velocity of flow:
V2 = (Cv)2 2gh or V = (Cv) 2gh
Wherein, V = velocity of flow Cv = coefficient of velocity
g = acceleration due to gravity (980 cm/sec/sec)
h = pressure gradient in cm of H2O
Combining these two equations, we get:
The final equation for calculation of valve orifice area A in cm2 is:
Where, CO = Cardiac output in cm3/ min
DFP = diastolic filling period (sec/beat)
SEP = systolic ejection period (sec/beat)
HR = heart rate (beats/min)
C = empirical constant
P = pressure gradient
You are setting up a PCR reaction. a) The total volume of your reaction is 50 uL. Your stock primer solutions are 25 mM each. You want a final concentration of primer of 1 mM in yo
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