Explain basic stains (cationic) - types of stains, Biology

Assignment Help:

Explain Basic Stains (Cationic) - Types of Stains?

These are chloride or sulfate salts of coloured bases which on ionization give positively charged chromogen. As such, these have a strong affinity for the negative constituents of the cell (or these bind strongly to negatively charged component of the cell), e.g. nucleic acid. Surface of the bacterial cells is also negatively charged, so basic dyes are most often used in bacteriology. These work better at high pH (i.e. neutral / alkaline conditions). For this reason, basic dyes are made up in alkaline solution, e.g. Loeffler's methylene blue stain is prepared in KOH solution.The pH can affect the staining effectiveness by affecting the nature and degree of charge on cell components. Whereas, in neutral or alkaline environment, microbial cells are negatively charged, in acidic environment, these stain poorly with basic dyes. Different staining techniques can be used to visualize and study the different morphological characteristics of a cell.


Related Discussions:- Explain basic stains (cationic) - types of stains

How folate is important for pregnant women, How Folate is Important For Pre...

How Folate is Important For Pregnant Women? Folate is also important for pregnant women. Low blood levels of folate during pregnancy can cause neural tube defects-anencephaly (

What is the salivary digestive enzyme, Q. What is the salivary digestive en...

Q. What is the salivary digestive enzyme? Which type of food does it digest and into which smaller molecules does it transform the food? The salivary hydrolase is known as sali

Carbon dioxide in the atmosphere, Which part of the carbon cycle is respons...

Which part of the carbon cycle is responsible for the enhancing concentration of carbon dioxide in the atmosphere. The burning of fossilized products (coal and oil) of some pla

Influence of diabetes mellitus, Influence of Diabetes Mellitus :  Diabetes...

Influence of Diabetes Mellitus :  Diabetes causes micro vascular obstructive disease in heart, kidney, retina and peripheral nerves. Scrupulous rather than casual control of diabe

Electrophoresis gel to load 60, Assume your tilapia fish muscle homogenate ...

Assume your tilapia fish muscle homogenate has a protein concentration of 5769 ?g/ml. You take 32.5 ?l of this fish homogenate, mix it with 12.5 ?l sample buffer and 5 ?l reducing

Define parts of coenzymes, As coenzymes participate in a variety of functio...

As coenzymes participate in a variety of functions, they can be classified broadly into two groups: i)  Hydrogen transferring  coenzymes, and ii)  Group transferring coenzym

Major conventional symbols & signs used in genetic family, What are the maj...

What are the major conventional symbols and signs used in genetic family trees? In the genetic family trees the male sex is usually represented by a square and the female by a

Explain calvin cycle, Calvin cycle (the aka Calvin-Benson Cycle or the Car...

Calvin cycle (the aka Calvin-Benson Cycle or the Carbon Fixation) is the series of biochemical, enzyme-mediated reactions during which the atmospheric carbon dioxide is reduced an

Toxoplasma cycles, Toxoplasma cycles between its rodent and feline hosts,li...

Toxoplasma cycles between its rodent and feline hosts,living out different phases of its existence in each. what is the scientific means about the cat and mouse.

Explain about the proteins, Explain about the Proteins? Proteins, as yo...

Explain about the Proteins? Proteins, as you may already know, are made up of carbon, hydrogen, nitrogen, oxygen and usually sulphur. Proteins are built up of a large number of

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd