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Autoxidation
It is generally agreed that "autoxidation", that is, the reaction with molecular oxygen via a self - catalytic mechanism, is the main reaction involved in oxidative deterioration of lipids. Autoxidation reaction can be divided into three parts:
a. initiation,
b. propagation, and
c. termination.
Assume your tilapia fish muscle homogenate has a protein concentration of 5769 ?g/ml. You take 32.5 ?l of this fish homogenate, mix it with 12.5 ?l sample buffer and 5 ?l reducing
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