Explain about the zone electrophoresis, Biology

Assignment Help:

Explain about the Zone Electrophoresis?

Zone electrophoresis which was introduced about a decade later describes the migration of charged macromolecules in a porous supporting medium such as cellulose paper, cellulose acetate sheets, and agarose gels. Zone electrophoresis differs from the moving boundary electrophoresis in that it produces an electrophoretogram, a display of protein zones, each one sharply separated from neighbouring zones, on the electrophoretic support material.

In the methods, electrophoresis is carried out in a buffered medium at a fixed pH so that the charge on the particles and hence the rate of migration is stabilized. However, complex mixtures of smaller molecular weight ampholytes can be prepared in which the passage of an electric current produces a gradual increase in the pH of the system from anode to cathode as the components become arranged in order of the increasing pH of their isoelectric points. Isoelectric focussing is based on this principle. When a mixture of high molecular weight ampholytes such as proteins is introduced into such a system covering a pH range of their isoelectric points, the molecules migrate towards the anode or cathode until they arrive at the point at which the pH is that of their isoelectric point. At this point, there is no net charge so the molecule then remains stationary. Isoenzymes and genetic variants can be finely resolved by this technique.


Related Discussions:- Explain about the zone electrophoresis

3x samples collected in process validation, To get the idea on the process ...

To get the idea on the process capability that whether the intended process provides the consistent results or not(precision).

Define the stability of your favourite protein kinase, You measure the stab...

You measure the stability of your favourite protein kinase and find that half of the protein is degraded every 10 minutes. How might you test whether the protein is degraded throug

What are the symptom of short bowel syndrome, Q. What are the Symptom of sh...

Q. What are the Symptom of short bowel syndrome? Diarrhoea is the main symptom of short bowel syndrome. Other symptoms include: • Anorexia • Steatorrhoea • Heart bum a

What are the main structures of chloroplasts, What are the main structures ...

What are the main structures of chloroplasts? Chloroplasts are included by two membrane layers, the outer and the inner membranes. Inside the organelle the formative unit is kn

Cardiac transplantation, For patients with end-stage heart failure, cardiac...

For patients with end-stage heart failure, cardiac transplantation has become a promising therapy especially with the advent of  immunosuppressive therapy and more careful screenin

Explain evidence of coverage of ecology, Explain Evidence of coverage of Ec...

Explain Evidence of coverage of Ecology Evidence of coverage of Ecology and Evolution to the level of Descriptor 2 and an evaluation or  discussion that shows depth of underst

Why is it advantageous to have weak hydrogen bonds, Why is it advantageous ...

Why is it advantageous to have weak hydrogen bonds among complementary bases and strong covalent bonds among phosphate and Deoxyribose groups in a DNA molecule? The hydrogen bo

Why fats have highest heat energy density, Why Fats have highest heat energ...

Why Fats have highest heat energy density? Fats have highest heat energy density of 9 Kcal/g. It is the major storage form of energy in body requiring least space and minimum w

Horizontal chart - organization chart, Horizontal Chart: Horizontal ch...

Horizontal Chart: Horizontal chart which read from left to right are occasionally used. The pyramid lies horizontally instead of standing in the vertical position. The line of

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd