Explain about the zone electrophoresis, Biology

Assignment Help:

Explain about the Zone Electrophoresis?

Zone electrophoresis which was introduced about a decade later describes the migration of charged macromolecules in a porous supporting medium such as cellulose paper, cellulose acetate sheets, and agarose gels. Zone electrophoresis differs from the moving boundary electrophoresis in that it produces an electrophoretogram, a display of protein zones, each one sharply separated from neighbouring zones, on the electrophoretic support material.

In the methods, electrophoresis is carried out in a buffered medium at a fixed pH so that the charge on the particles and hence the rate of migration is stabilized. However, complex mixtures of smaller molecular weight ampholytes can be prepared in which the passage of an electric current produces a gradual increase in the pH of the system from anode to cathode as the components become arranged in order of the increasing pH of their isoelectric points. Isoelectric focussing is based on this principle. When a mixture of high molecular weight ampholytes such as proteins is introduced into such a system covering a pH range of their isoelectric points, the molecules migrate towards the anode or cathode until they arrive at the point at which the pH is that of their isoelectric point. At this point, there is no net charge so the molecule then remains stationary. Isoenzymes and genetic variants can be finely resolved by this technique.


Related Discussions:- Explain about the zone electrophoresis

What is the essential morphology of a protozoan cell, Q. What is the essent...

Q. What is the essential morphology of a protozoan cell? Protozoans are eukaryotic cells so they have structures and organelles common to this kind of cell endoplasmic reticula

Global air circulation, Global Air Circulation The major wind systems o...

Global Air Circulation The major wind systems of the earth result because large masses of air around the earth's equator are forced to rise from the bottom due to surface heati

Explain the structure and functions of kidney, Explain the Structure and Fu...

Explain the Structure and Functions of Kidney You would have got a clear idea as to where the kidneys are situated. Yes, the kidneys are located in the posterior part of the ab

Explain terms of osmosis, A red blood cell has an internal salt concentrati...

A red blood cell has an internal salt concentration of 150mM. The cell is placed in a beaker of 500mM salt. (a) Assuming that the cell membrane is permeable to water but not to

Ecretory system, excretory product of marine teleost

excretory product of marine teleost

Explain what is inversion, Explain what is Inversion ? Inversion is ano...

Explain what is Inversion ? Inversion is another way in which genetic recombination can occur, as seen below. In this case, the change occurs within a chromosome, rather than b

Explain the periodontal consideration, Explain the Periodontal consideratio...

Explain the Periodontal consideration Periodontal consideration would include: Hard Calculus Build up:   Any index assessing the amount of calculus build up can be used .

Determine the food-searching activity, The Svalbard ptarmigan (Lagopus mutu...

The Svalbard ptarmigan (Lagopus mutus hyperboreus) is a small herbivorous bird that lives permanently on the island chain of Svalbard. Svalbard is located in the Arctic Circle, app

Systemic emboli, Osler's nodes are small, tender subcutaneous nodules that ...

Osler's nodes are small, tender subcutaneous nodules that develop in the pulp of the digits or occasionally more proximally in the fingers and persist for hours to several days. Th

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd