Explain about the zone electrophoresis, Biology

Assignment Help:

Explain about the Zone Electrophoresis?

Zone electrophoresis which was introduced about a decade later describes the migration of charged macromolecules in a porous supporting medium such as cellulose paper, cellulose acetate sheets, and agarose gels. Zone electrophoresis differs from the moving boundary electrophoresis in that it produces an electrophoretogram, a display of protein zones, each one sharply separated from neighbouring zones, on the electrophoretic support material.

In the methods, electrophoresis is carried out in a buffered medium at a fixed pH so that the charge on the particles and hence the rate of migration is stabilized. However, complex mixtures of smaller molecular weight ampholytes can be prepared in which the passage of an electric current produces a gradual increase in the pH of the system from anode to cathode as the components become arranged in order of the increasing pH of their isoelectric points. Isoelectric focussing is based on this principle. When a mixture of high molecular weight ampholytes such as proteins is introduced into such a system covering a pH range of their isoelectric points, the molecules migrate towards the anode or cathode until they arrive at the point at which the pH is that of their isoelectric point. At this point, there is no net charge so the molecule then remains stationary. Isoenzymes and genetic variants can be finely resolved by this technique.


Related Discussions:- Explain about the zone electrophoresis

What is panspermia, What is panspermia? The Panspermia is a hypothesis ...

What is panspermia? The Panspermia is a hypothesis that describes life on earth as not originated from the planet. The thought is that the first living beings that colonized th

Nitrates and nitrites- preservative, Normal 0 false false f...

Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4

Bones, which bone forms the non-moving muscle attachment in the hip joint

which bone forms the non-moving muscle attachment in the hip joint

Pbpk modeling during human drug development, Early human studies are normal...

Early human studies are normally conducted in healthy subjects to assess acute safety and, if applicable, to monitor biochemical and pharmacological responses. These studies also p

Phylum Protozoa, salient features of phylum Protozoa

salient features of phylum Protozoa

Explain vasopressindrug term, Explain Vasopressindrug term? As a single...

Explain Vasopressindrug term? As a single bolus of 40 U, vasopressin has been recommended as an alternative to epinephrine in VF/pulseless VT refractory to defibrillation. Furt

What do carbon chains common with a skeletal system, What do carbon chains ...

What do carbon chains have in common with a skeletal system?

Explain the scaling from individuals to ecosystems, Explain the Scaling fro...

Explain the Scaling from individuals to Ecosystems? Models that explain how individual organisms acquire energy and materials, and how they use them for survival, growth and re

Neurotransmitters to neuromuscular junctions, Blood functions to maintain h...

Blood functions to maintain homeostasis in the human body through all but which of the following: Answer moving carbon dioxide away from cells following completion of aerobic metab

Explain cori cycle , Under the limiting oxygen conditions experienced in ...

Under the limiting oxygen conditions experienced in by vigorous exercise, the configuration of NADH by glycolysis exceeds the ability of the respiratory chain to oxidize it back to

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd