Explain about the zone electrophoresis, Biology

Assignment Help:

Explain about the Zone Electrophoresis?

Zone electrophoresis which was introduced about a decade later describes the migration of charged macromolecules in a porous supporting medium such as cellulose paper, cellulose acetate sheets, and agarose gels. Zone electrophoresis differs from the moving boundary electrophoresis in that it produces an electrophoretogram, a display of protein zones, each one sharply separated from neighbouring zones, on the electrophoretic support material.

In the methods, electrophoresis is carried out in a buffered medium at a fixed pH so that the charge on the particles and hence the rate of migration is stabilized. However, complex mixtures of smaller molecular weight ampholytes can be prepared in which the passage of an electric current produces a gradual increase in the pH of the system from anode to cathode as the components become arranged in order of the increasing pH of their isoelectric points. Isoelectric focussing is based on this principle. When a mixture of high molecular weight ampholytes such as proteins is introduced into such a system covering a pH range of their isoelectric points, the molecules migrate towards the anode or cathode until they arrive at the point at which the pH is that of their isoelectric point. At this point, there is no net charge so the molecule then remains stationary. Isoenzymes and genetic variants can be finely resolved by this technique.


Related Discussions:- Explain about the zone electrophoresis

Explain cardiac examination auscultation and its techniques, Explain cardia...

Explain cardiac examination auscultation and its techniques? Auscultation of the heart sound and associatcd sound is very important to diagnose the cardiovascular diseases. It

Explain microbiology of fruits, Q. Explain Microbiology of fruits? Ans...

Q. Explain Microbiology of fruits? Ans. Fresh fruits, like vegetables, are in a close vicinity of ground and hence may have varied flora of microorganisms. Nature has

Symmetry, what is the differences between spherical symmetry and radial sym...

what is the differences between spherical symmetry and radial symmetry

Eyeball - sclerotic, SCLEROTI C - White of eye. Outer thick, made u...

SCLEROTI C - White of eye. Outer thick, made up of fibres connective tissue. Protective layer. Give definite shape. Its anterior 1/6 part is thin, transparent known as

Define requirements of fat in postoperative nutritional care, Define Requir...

Define Requirements of Fat in Postoperative Nutritional Care Adequate amount of fat is needed to build up and maintain tissue fat reserves. Depending upon the existing health a

Determine about the trichromatic vision, Determine about the Trichromatic v...

Determine about the Trichromatic vision Trichromatic vision is the terminology used for normal vision, those who require aU' three primary colours to make a match with an unkno

Methods of evaluation, METHODS OF EVALUATION: The two main areas of ev...

METHODS OF EVALUATION: The two main areas of evaluation include a) evaluation of  job performance of individual b) evaluation  of programme  agency  1)  Evaluation of  the

Conditions required for mimicking pericardial effusion, Q. Conditions requi...

Q. Conditions required for Mimicking Pericardial Effusion? In 2DE few conditions can mimic PE. a) Pericardial fat-Mostly localized anteriorly. Absence of fat in the posterio

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd