Explain about the zone electrophoresis, Biology

Assignment Help:

Explain about the Zone Electrophoresis?

Zone electrophoresis which was introduced about a decade later describes the migration of charged macromolecules in a porous supporting medium such as cellulose paper, cellulose acetate sheets, and agarose gels. Zone electrophoresis differs from the moving boundary electrophoresis in that it produces an electrophoretogram, a display of protein zones, each one sharply separated from neighbouring zones, on the electrophoretic support material.

In the methods, electrophoresis is carried out in a buffered medium at a fixed pH so that the charge on the particles and hence the rate of migration is stabilized. However, complex mixtures of smaller molecular weight ampholytes can be prepared in which the passage of an electric current produces a gradual increase in the pH of the system from anode to cathode as the components become arranged in order of the increasing pH of their isoelectric points. Isoelectric focussing is based on this principle. When a mixture of high molecular weight ampholytes such as proteins is introduced into such a system covering a pH range of their isoelectric points, the molecules migrate towards the anode or cathode until they arrive at the point at which the pH is that of their isoelectric point. At this point, there is no net charge so the molecule then remains stationary. Isoenzymes and genetic variants can be finely resolved by this technique.


Related Discussions:- Explain about the zone electrophoresis

Blood vitreous barrier as the physio-chemical property, Explain the blood v...

Explain the blood vitreous barrier as the physio-chemical property. Blood Vitreous Barrier: The blood vitreous barrier consists of three components: Tight junctional comp

Events after sperm and egg fusion, Events after Sperm and Egg Fusion ...

Events after Sperm and Egg Fusion The entry of the sperm into the egg activates the egg. The activation of egg results in two types of responses. (1) The early responses a

Nutrition, definition of dietary factors. or what are dietary factors?

definition of dietary factors. or what are dietary factors?

Excretion, what is the excretory organ of lizard

what is the excretory organ of lizard

Method of dna techonology tp improve lives of humans, List three ways that ...

List three ways that DNA technology could be used to improve the lives of humans. Answers could include: identifying human remains, determining a person's paternity, tracing h

Exercise and medications in bronchial asthma, Exercise Stress or em...

Exercise Stress or emotional upset  Medications Aspirin, non-steroidal anti-inflammatory drugs(NSAIDs), beta- blockers(including eye drops), cholinergic dr

How are radioactive probes useful in dna fingerprinting, How are radioactiv...

How are radioactive probes useful in DNA fingerprinting? Radioactive probes bind to specific fragments in a DNA sample like a donor gene. A probe can identify which bacteria ha

Mechanism of circulatory system, MECHANISM - 1 .      AURICULAR SYSTO...

MECHANISM - 1 .      AURICULAR SYSTOLE - First of all from complete body impure blood comes in R.A. by superior and inferior vena cava or pre and post cavals. Pure blo

Pathogenesis, Even though association between GAS pharyngitis and the ARF i...

Even though association between GAS pharyngitis and the ARF is fairly well established, the exact pathogenic mechanisms are not clearly understood. However, two mechanisms are post

Principles of doppler echocardiography, Doppler echocardiogaphy is based on...

Doppler echocardiogaphy is based on  the Doppler effect, which was  described by  the Austrian phy$icist Christian Doppler in  1842. The Doppler effect states that sound frequency

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd