Explain about the zone electrophoresis, Biology

Assignment Help:

Explain about the Zone Electrophoresis?

Zone electrophoresis which was introduced about a decade later describes the migration of charged macromolecules in a porous supporting medium such as cellulose paper, cellulose acetate sheets, and agarose gels. Zone electrophoresis differs from the moving boundary electrophoresis in that it produces an electrophoretogram, a display of protein zones, each one sharply separated from neighbouring zones, on the electrophoretic support material.

In the methods, electrophoresis is carried out in a buffered medium at a fixed pH so that the charge on the particles and hence the rate of migration is stabilized. However, complex mixtures of smaller molecular weight ampholytes can be prepared in which the passage of an electric current produces a gradual increase in the pH of the system from anode to cathode as the components become arranged in order of the increasing pH of their isoelectric points. Isoelectric focussing is based on this principle. When a mixture of high molecular weight ampholytes such as proteins is introduced into such a system covering a pH range of their isoelectric points, the molecules migrate towards the anode or cathode until they arrive at the point at which the pH is that of their isoelectric point. At this point, there is no net charge so the molecule then remains stationary. Isoenzymes and genetic variants can be finely resolved by this technique.


Related Discussions:- Explain about the zone electrophoresis

Protein synthesis, Protein Synthesis The central dogma of modern bioche...

Protein Synthesis The central dogma of modern biochemistry is totally based on the coded information holds within deoxyribonucleic acid (DNA). Double stranded DNA is converted

Define determinants of food security - vulnerability, Define Determinants o...

Define Determinants of Food Security - Vulnerability? It is the fourth critical variable and defines the risk factor to which a person, family, community or nation is exposed o

Nursing assessment of acute glomerulonephritis, Nursing Assessment   Du...

Nursing Assessment   During assessment the child's parents will give you history of upper respiratory infection or skin infection  in the previous one to three weeks. Initially

Define the term- brain circuitry underlying addiction, Define the term- bra...

Define the term- brain circuitry underlying addiction By unraveling brain circuitry underlying addiction, scientists have identified new targets for therapies which might quell

Pathogenesis of viruses, Pathogenesis of Viruses Viral diseases An...

Pathogenesis of Viruses Viral diseases An indicated before, viruses cause a number of infectious human diseases from minor ailments like common cold, to fatal disease, lik

Explain about implant surgery, Explain about implant surgery Diagnosis ...

Explain about implant surgery Diagnosis and treatment planning for a patient who requires and is to receive dental implants. The actual process of dental implant placement begi

Conventional cabg on cardiopulmonary bypass, Conventional CABG on Cardiopul...

Conventional CABG on Cardiopulmonary Bypass   Chest is opened by midline incision and median sternotomy. Simultaneously saphenous vein or radial artery is harvested. 'T

Cryptosporidiosis, Cryptosporidiosis Cryptosporidiosis, a protozoan diseas...

Cryptosporidiosis Cryptosporidiosis, a protozoan disease of the intestinal tract, is caused by Cryptosporidium parvum and Cryptosporidium muris. It infects man, dog, cat, calves,

Osmoregulation in marine non-chordate metazoans, Osmoregulation in Marine N...

Osmoregulation in Marine Non-Chordate Metazoans Studies on the osmotic pressure of body fluids of marine organisms have displayed that their internal osmotic pressure is more

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd