Explain about the zone electrophoresis, Biology

Assignment Help:

Explain about the Zone Electrophoresis?

Zone electrophoresis which was introduced about a decade later describes the migration of charged macromolecules in a porous supporting medium such as cellulose paper, cellulose acetate sheets, and agarose gels. Zone electrophoresis differs from the moving boundary electrophoresis in that it produces an electrophoretogram, a display of protein zones, each one sharply separated from neighbouring zones, on the electrophoretic support material.

In the methods, electrophoresis is carried out in a buffered medium at a fixed pH so that the charge on the particles and hence the rate of migration is stabilized. However, complex mixtures of smaller molecular weight ampholytes can be prepared in which the passage of an electric current produces a gradual increase in the pH of the system from anode to cathode as the components become arranged in order of the increasing pH of their isoelectric points. Isoelectric focussing is based on this principle. When a mixture of high molecular weight ampholytes such as proteins is introduced into such a system covering a pH range of their isoelectric points, the molecules migrate towards the anode or cathode until they arrive at the point at which the pH is that of their isoelectric point. At this point, there is no net charge so the molecule then remains stationary. Isoenzymes and genetic variants can be finely resolved by this technique.


Related Discussions:- Explain about the zone electrophoresis

What is an example of evolutionary novelty, Compared to amphibians what is ...

Compared to amphibians what is an example of evolutionary novelty present in beings of the class Reptilia against the loss of water through the skin? The reptile skin is kerat

External auditors reports, External Auditors Reports The audit report...

External Auditors Reports The audit report is the final stage in the process of an audit.  The terms 'true and fair'  'present fairly' have special significance in the audit

What is a goiter, What is a goiter? What is endemic goiter? How is this pro...

What is a goiter? What is endemic goiter? How is this problem socially solved? Goiter is the abnormal enlargement of the thyroid gland. The goiter appears as a tumor in the ant

Benefits of high-throughput expression , What are the benefits of high-thro...

What are the benefits of high-throughput expression analysis in molecular biological investigations? Ans) These methods permits simultaneous analysis of the expression of a lot

Financing of healthcare - Principles, Normal 0 false false ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Define diagnostic criteria for anorexia nervosa, Define Diagnostic Criteria...

Define Diagnostic Criteria for Anorexia Nervosa? Table gives the American Psychiatric Association diagnostic criteria for anorexia nervosa. Although the manual does not use bod

What do biomass pyramids represent, What do biomass pyramids represent? ...

What do biomass pyramids represent? The Biomass pyramids represent the sum of the masses of the individuals that participate in each trophic level of a food chain.

Endocrine glands, Endocrine glands Endocrine glands have no ducts - ...

Endocrine glands Endocrine glands have no ducts - so these are also called as ductless glands Eg.Pituitary etc., These glands secrete chemical substances called HORMONES

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd