Explain about the zone electrophoresis, Biology

Assignment Help:

Explain about the Zone Electrophoresis?

Zone electrophoresis which was introduced about a decade later describes the migration of charged macromolecules in a porous supporting medium such as cellulose paper, cellulose acetate sheets, and agarose gels. Zone electrophoresis differs from the moving boundary electrophoresis in that it produces an electrophoretogram, a display of protein zones, each one sharply separated from neighbouring zones, on the electrophoretic support material.

In the methods, electrophoresis is carried out in a buffered medium at a fixed pH so that the charge on the particles and hence the rate of migration is stabilized. However, complex mixtures of smaller molecular weight ampholytes can be prepared in which the passage of an electric current produces a gradual increase in the pH of the system from anode to cathode as the components become arranged in order of the increasing pH of their isoelectric points. Isoelectric focussing is based on this principle. When a mixture of high molecular weight ampholytes such as proteins is introduced into such a system covering a pH range of their isoelectric points, the molecules migrate towards the anode or cathode until they arrive at the point at which the pH is that of their isoelectric point. At this point, there is no net charge so the molecule then remains stationary. Isoenzymes and genetic variants can be finely resolved by this technique.


Related Discussions:- Explain about the zone electrophoresis

What is gel, What is Gels Sol is free flowing liquid at room temperatur...

What is Gels Sol is free flowing liquid at room temperature, when it becomes relatively firm, it is called a gel. There are a number of factors that affect the strengths, elast

Explain some handy points related to infant feeding, Explain some Handy Poi...

Explain some Handy Points related to Infant Feeding? Points to be kept in mind: • Introduce only one food at a time, giving only small amounts at first • Increase variety s

What are similarities between peroxisomes and lysosomes, Q. What are the mo...

Q. What are the morphological, chemical and functional similarities and differences between peroxisomes and lysosomes? Similarities: Peroxisomes and lysosomes are small membran

Define classification of carbohydrates - monosaccharide, Define Classificat...

Define Classification of Carbohydrates - Monosaccharide? Monosaccharides or simple sugars are those carbohydrates that cannot be hydrolyzed into simpler carbohydrates. E.g. gl

Movement of molecules into and out of a cell, how does a plasma membrane re...

how does a plasma membrane regulate movement of molecules into and out of a cell? is it polarity, integrity, permeability, or solubility? these are my choices

Diffusion of gases, Diffusion of Gases Gases diffuse from areas of hi...

Diffusion of Gases Gases diffuse from areas of higher partial pressure to areas of lower partial pressure. The diffusion along the partial pressure gradient ceases only when

Definition of infective endocarditis, Infective endocarditis (IE) is a micr...

Infective endocarditis (IE) is a microbial infection of the endothelial surface of the heart. The characteristic lesion, the vegetation, is a variably sized amorphous mass of plate

Social determinants of health - transport, Social Determinants of Health - ...

Social Determinants of Health - Transport Rising worldwide incomes have led to a massive use of automobiles and in particular cars. Cars are seen as a threat to health because

Explain repressors , Gene repressor proteins which inhibit the trans...

Gene repressor proteins which inhibit the transcription of particular genes in eukaryotes also exist. They may act by binding either to control parts within the promoter region nea

Geology LAB, Can you guys help with my homework assignment for Geology?

Can you guys help with my homework assignment for Geology?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd