Explain about the zone electrophoresis, Biology

Assignment Help:

Explain about the Zone Electrophoresis?

Zone electrophoresis which was introduced about a decade later describes the migration of charged macromolecules in a porous supporting medium such as cellulose paper, cellulose acetate sheets, and agarose gels. Zone electrophoresis differs from the moving boundary electrophoresis in that it produces an electrophoretogram, a display of protein zones, each one sharply separated from neighbouring zones, on the electrophoretic support material.

In the methods, electrophoresis is carried out in a buffered medium at a fixed pH so that the charge on the particles and hence the rate of migration is stabilized. However, complex mixtures of smaller molecular weight ampholytes can be prepared in which the passage of an electric current produces a gradual increase in the pH of the system from anode to cathode as the components become arranged in order of the increasing pH of their isoelectric points. Isoelectric focussing is based on this principle. When a mixture of high molecular weight ampholytes such as proteins is introduced into such a system covering a pH range of their isoelectric points, the molecules migrate towards the anode or cathode until they arrive at the point at which the pH is that of their isoelectric point. At this point, there is no net charge so the molecule then remains stationary. Isoenzymes and genetic variants can be finely resolved by this technique.


Related Discussions:- Explain about the zone electrophoresis

Define tests for the presence of exoenzymatic activity, Define Tests for th...

Define Tests for the Presence of Exoenzymatic Activity? Microorganisms require various micro- and macro-nutrients for energy production and growth. These are obtained from the

Explain vitamins requirement during thyphoid, Explain Vitamins requirement ...

Explain Vitamins requirement during thyphoid Vitamins : Vitamins which need to be emphasized include B complex, considering the increase  in  the energy requirement and a decr

Male reproductive disorders-testicular hypoplasia, Testicular Hypoplasia ...

Testicular Hypoplasia Hypoplasia refers to testicular disorders related to congenitally underdeveloped and underactive spermatic epithelium of one or both testes even though t

Explain the culture media and its types, Explain the Culture Media and Its ...

Explain the Culture Media and Its Types? A culture medium (Pl. media), we already know, is a solid or liquid preparation containing all the nutrients required by microorganisms

Essentials of Human Diseases and Conditions, How do pathological conditions...

How do pathological conditions affect homeostasis of your body?

How peroxisomes formed, How Peroxisomes formed Peroxisomes are formed w...

How Peroxisomes formed Peroxisomes are formed within the cell from the smooth endoplasmic reticulum by a process of budding. Peroxisomes found in the kidney and liver cells are

Explain about phytopinax written by caspar bauhin, Explain about Phytopinax...

Explain about Phytopinax written by Caspar Bauhin? A significant contribution to taxonomy was made at this time by Caspar Bauhin. His 'Phytopinax' (1596) described 2700 species

Heart and lungs, what are the main functions of lungs and heart

what are the main functions of lungs and heart

Properties of isozymes, Properties of Isozymes Isozymes differ from  ...

Properties of Isozymes Isozymes differ from  each other not only in  the amino acid composition, they also have different electrophoretic property, thermolability, immunologi

Psychology, What name is given to the mental processing of sensory informat...

What name is given to the mental processing of sensory information?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd