Explain about the zone electrophoresis, Biology

Assignment Help:

Explain about the Zone Electrophoresis?

Zone electrophoresis which was introduced about a decade later describes the migration of charged macromolecules in a porous supporting medium such as cellulose paper, cellulose acetate sheets, and agarose gels. Zone electrophoresis differs from the moving boundary electrophoresis in that it produces an electrophoretogram, a display of protein zones, each one sharply separated from neighbouring zones, on the electrophoretic support material.

In the methods, electrophoresis is carried out in a buffered medium at a fixed pH so that the charge on the particles and hence the rate of migration is stabilized. However, complex mixtures of smaller molecular weight ampholytes can be prepared in which the passage of an electric current produces a gradual increase in the pH of the system from anode to cathode as the components become arranged in order of the increasing pH of their isoelectric points. Isoelectric focussing is based on this principle. When a mixture of high molecular weight ampholytes such as proteins is introduced into such a system covering a pH range of their isoelectric points, the molecules migrate towards the anode or cathode until they arrive at the point at which the pH is that of their isoelectric point. At this point, there is no net charge so the molecule then remains stationary. Isoenzymes and genetic variants can be finely resolved by this technique.


Related Discussions:- Explain about the zone electrophoresis

How is the yolk sac formed, How is the yolk sac formed? What is the functio...

How is the yolk sac formed? What is the function of the yolk sac? The yolk sac is formed from the covering of the vitellus by some cells originated from the primitive gut. T

Types of cell respiration, Q What are the kinds of cell respiration? Th...

Q What are the kinds of cell respiration? There are two types of cell respiration aerobic cell respiration a reaction with participation of molecular oxygen (O2) and anaerobic

Difference between temporal summation and spatial summation, Q. What is the...

Q. What is the difference between temporal summation and spatial summation of muscle fibers? What is tetany?   Spatial summation is the recruiting of new muscle fibers to incr

Define population size, Define Population size, inbreeding and genetic vari...

Define Population size, inbreeding and genetic variation? "Darwin proposed, and modern evolutionary biology affirms, that evolution is relies on variation in the characteristic

Explain about the spinal trauma, Explain about the Spinal Trauma? Spina...

Explain about the Spinal Trauma? Spinal trauma or spinal cord injury, commonly clue to accidents, fills, sports injury can result in serious disabling consequences. The spinal

Zoology, why protozoass dont have vaccines esp. plasmodiums

why protozoass dont have vaccines esp. plasmodiums

What happens within the cell in the first interface period, What are the ev...

What are the events that mark the beginning and the end of the first interphase period? What happens within the cell in this period? The first interphase period is the G1. It s

Responses of plants to stress-physiology, Responses Of Plants To Stress ...

Responses Of Plants To Stress The internal chemical signals and environmental factors - the external signals which are perceived by special molecules present in the cell. In t

Describe about the anemia and its type, Q. What is the anemia? What are the...

Q. What is the anemia? What are the four major types of anemia? Anemia is low concentration of hemoglobin in the blood, the four major kind of anemia are the nutrient-deficienc

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd