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Explain about the Gel bands detection techniques?
Gel bands, resulting from a gel electrophoretic separation, may be detected by staining, radioactive counting or immunoblotting. Proteins in microgram quantities are often visualized by staining with Coomassie Brilliant Blue. Gel bands with lesser amount of protein can be visualized with silver stain which is 50 times more sensitive. Fluorescamine an alternative type of protein stain is a non- fluorescent molecule that reacts with primary amines, such as lysine, to yield an addition product that is highly fluorescent under UV irradiation. Proteins, DNA etc. can be detected through the UV absorption of a gel along its length.
If the sample is radioactive, the gel may dry under vacuum after wrapping in cellophane sheet and then damped over a sheet of X-ray film. After some time (few minutes to many weeks depending on the radiation intensity), the film is developed and the resulting autoradiograph shows the position of the radioactive components on 3. Enumerate the different types of electrophoresis.latter method yields quantitatively more accurate results than autoradiography. If an antibody to the protein of interest is available, it is possible to specifically detect this protein on the gel in the presence of many other proteins by immunoblot or western blot. With this brief review we move over to the last electrophoresis method i.e. disc electrophoresis.
List a few important uses of food hydrocolloids. A few important uses of food hydrocolloids are: to increase viscosity and to stabilize food products. Its other uses include u
Endosperm with Lateral Haustoria In Monochoria, in which the endosperm development is of helobial type, the haustorium is neither chalazal nor micropylar but lateral. The cha
Explain the Toxicity of Vitamin D? The adverse effects of high vitamin D intakes include hypercalciuria (excessive urinary calcium excretion) and hypercalcaemia (high concentr
(a) Explain the structure of a human sperm. (b) Give a schematic representation showing the events of Spermatogeninesis in human male.
Factors affecting lipid oxidation Temperature In general, the rate of oxidation increases as the temperature is increased. Temperature also influences the relation betw
Give the salient features of phylum protozoa
Salmonellosis Salmonellosis is an infection caused by bacterial species belonging to the genus Salmonella and usually associated with the ingestion of food containing salmonel
What is the constitution of the cartilaginous matrix? The cartilaginous matrix is made of collagen fibers, mostly collagen type II, and of proteoglycans, proteins associated to
Calculate the osmolarity of a solution of 5% dextrose in water (dextrose = glucose, molec. Wt. = 180).
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