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Ethidium Bromide intercalates within structure of the nucleic acids in such a manner that they fluoresce under the UV light. Ethidium bromide staining is generally used to visualize RNA or DNA in the agarose gels placed on the UV light boxes. Proper precautions are needed, because the ethidium bromide is extremely mutagenic and the UV light damaging to eyes. Ethidium bromide is also involved in cesium chloride gradients during the process of ultracentrifugation, to separate the supercoiled circular DNA from linear and the relaxed circular DNA.
Define Functions of Manganese? Like other micro minerals, Mn also functions in mammalian enzyme systems. It can function both as an integral part of metalloenzymes and as an en
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HACCP - What is Validation Validation : That element of verification focused on collecting and evaluating scientific and technical information to determine if the HACCP
Excretion in Non-Chordates Excretion as described under Introduction of this unit refers to removal of the waste products of metabolism - carbon dioxide (CO 2 ) and water rele
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How do carrier proteins transport substances across cell membranes? Carrier proteins bind to a molecule of the substance on single side of the membrane, change shape, transpor
To synthesize glycogen two enzymes are needed: 1. UDP-glucose pyrophosphorylase catalyzes the synthesis of UDP-glucose from UTP and glucose 1-phosphate: The py
Q. What is the ADP phosphorylation? What respectively are oxidative phosphorylation and photophosphorylation? ADP phosphorylation is the addition of one inorganic phosphate in
Describe Alternation of Generations? Alternation of Generations : In meiosis, four haploid daughter cells are formed from one diploid mother cell. The life cycles of sexuall
Plasma Proteins - Circulation The total protein concentration of the plasma is around 7% only. The plasma proteins are of three types - serum albumin (4%), serum globulin (2.7
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