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Ethidium Bromide intercalates within structure of the nucleic acids in such a manner that they fluoresce under the UV light. Ethidium bromide staining is generally used to visualize RNA or DNA in the agarose gels placed on the UV light boxes. Proper precautions are needed, because the ethidium bromide is extremely mutagenic and the UV light damaging to eyes. Ethidium bromide is also involved in cesium chloride gradients during the process of ultracentrifugation, to separate the supercoiled circular DNA from linear and the relaxed circular DNA.
1. Define each term and give an example of an application that uses the following: a. Biocompatible material b. Biodegradable material c. Biomimetic
I will send any further instructions to you. The ist part of the assessment (1-7) that evaluates the chart i have done but it needs to be looked at to see if content okay & gram
Porcine rotavirus Piglet diarrhoea and mortality are gaining importance and the viral agent which is highly incriminated in such episodes is porcine rotavirus. The clinical featur
Q. What is the difference between facilitated and simple diffusion? Facilitated by which kind of molecule does the term "facilitated" mean? Simple diffusion is the direct passa
How do you determine net atp production after complete oxidation to CO2 and H2O using mitochondrial ?-oxidation, the TCA cycle, the mitochondrial electron transport chain and oxida
During a fever in a human A. shivering may occur when the actual body temperature is lower than the set point for body temperature during the fever. B. there is an inc
using a specific example describe how hormones have an important role in the regulation of body functions and also, what happens if there is an over secretion or an under secretion
Problems with untreated/ammoniated crop residues Besides the low energy and protein contents, crop residues generally contain low content of minerals such as Calcium, Phosphor
Explain Precautions for Gram Staining of Bacterial Cultures? 1. The dilutions should be chosen on the basis of expected counts in the food sample. 2. Food should be kept in
Autotrophic and Heterotrophic Succession The succession where initially the green plants are much greater in quantity than the animals, is known as autotrophic succession. Su
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