Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Establishment of Suspension Cultures
A callus crumbles into smaller clumps and single cells in liquid medium by gentle agitation (100-120 rPM) on a shaker. Shaking the cultures also helps to aerate the P - cells. Such suspension cultures however rarely comprise single cells alone because cells tend to aggregate in clusters of 2-100. Suspension cultures can be maintained indefinitely by inoculations of known aliquot5 of cells to a fresh medium. This process is termed as "batch cultures". Alternatively, the medium is replenished at regular intervals. This process is termed as "continuous culture". In the continuous culture process at the time of replenishing the medium, cells are also harvested (open continuous system) or the biomass is allowed to increase (close continuous system).
Suspension cultures are useful in studying problems related to cell biology including cell cycle and production of secondary metabolites like alkaloids, steroids, glycosides, naphthoquinones, flavones etc. which find medicinal and industrial application. Pharmaceutical industries use large bioreactors for suspension cultures to obtain valuable bioorganic compounds. A bioreactor is a vessel of glass or steel in which cells are cultured aseptically and culture conditions are closely monitored. This results in higher yield of metabolites. In a bioreactor there is provision for adding fresh medium, for harvesting cells, for the aeration of products, for mixing and sampling, for controlling pH, O2 content and temperature Plant cells are immobilised in alginate, agarose, polyacrylamide beads.
This species is an ornamental tree in China, and has yielded the clinically active agents topotecan, irinotecan, and 9 -aminocamptothecin, which are semisynthetically derived from
general characteristic of phylum protozoa
Explain radiation sterilization Various studies have been conducted which show the effect of processing on vitamins especially, thiamine. In one study, which compared the effec
Define the Theory or Principle of Drying? Drying can be illustrated as the application of heat under controlled conditions to eliminate the majority of the water generally avai
Question 1 Discuss how would you perform antiglobulin test? Add a note on it clinical importance. What are the precautions to be taken while performing the test? Question 2 E
Explain about the Bifidogenic Effect? Fructans selectively stimulate the growth of Bifidobacteria and Lactobacilli, while decreasing concentrations of E.coli, Clostridia and ba
PHYSICAL STATE OF PROTOPLASM Several theories have been given about its physical structure - (i ) Granular Theory (Proposed by Altman, Hanstein, 1886) - Granules embed
Hypertensive emergencies are one of the important categories of hypertension and characterized by severe elevations in BP that are complicated by evidence of progressive target org
Define classification of carbohydrates - Oligosaccharides? Oligosaccharides are short chains of saccharide units and are condensation products of three to ten monosaccharides.
Amino acid sequence is also known as the primary structure of a protein/polypeptide; the series of amino acids in a protein/polypeptide controlled by the series of DNA bases.
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd