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If an enzyme-controlled reaction normally takes place at 10ºC, in general terms how will the reaction be affected by (a) a fall in temperature to 2°C , (b) a rise in temperature to 20°C. (c) a rise in temperature to 65°C?
If an enzyme normally works at 10°C, then
(a) A fall in temperature to 2°C will slow down the reaction
(b) A rise in temperature to 20°C will speed up the reaction (by x2)
(c) A rise in temperature to 65°C will denature the enzyme and stop it working (by the reaction might be speed up at first).
Determine the net charge of the predominant form Asp at (a) pH 1.0, (b) pH 3.0, (c) pH 6.0, and (d) pH 11.0?
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