Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
This technique utilizes an enzyme resolvase, endo vii, cloned from the bacteriophage t4. This enzyme has high specificity to find deletions, insertions, and base substitutions mutations or any mismatches. First the pcr is utilized to intensify the normal and mutant allele of targeted sequence, these pcr primers are labeled with fam(6-carboxyfluoroscein amidite) which is a blue fluorescent dye and reverse primer with tet(4,7,2,7-tetra chloro-6-carboxy fluoroscein) amidite which is a green fluorescent dye.
Denaturation and renaturation of normal and mutant allele in a mixture is made, consequently mismatch heteroduplexes will form about 50 % of the time, each and every base pair creates two mismatch. The endo vii enzymes then scan the double stranded dna until it finds structural distortion, either a bubble made by single base pair mutations or a hetero duplex loop created by hybridization of a wild type allele with a mutant allele having an insertion or deletion. The enzyme cleaves with in 6 base pair on 3"side of mutation creating two smaller fragments, one blue and the other one green. The dna sequence is analyzed on automated dna sequence and mobility of every fragment is analyzed.
Q. What are some human diseases caused by virus and what are their respective modes of transmission? The major viral diseases transmitted by respiratory secretions (cough, snee
What neurotransmitter is released from the preganglionic parasympathetic neurons
my homework is asking me to find the four possible gametes for two parents using their genotypes. How do i do that???? would I use the F.O.I.L. method?
Actinomyces Members of the genus Actinomyces are facultative anerobic, gram-positive, non acid-fast, non-spore forming, nonhaemolytic rods. All Actinomyces require rich media
Prosthodontic considerations In order to achieve good results, understated features should always be examinated. 1. Smile Line: A high smile line increases the esthetic
The formation of surface blebs caused by ischemia reflects in part plasma membrane injury, but also a sign of cytoplasmic injury. Which filamentous component of the cytoplasm plays
what are fats
You may have heard in the news currently that a new species of frog was discovered within a rainforest. You objective are to isolate genomic DNA and construct an expression libr
Q. Treatment of congestive cardiac failure? A judicious and careful co-ordination between oxygen support, drug therapy and nutrient intake can help in alleviating majority of t
Histogenesis and Organogenesis After the octant stage numerous cell divisions occur in various planes. The proembryo become globular or bulb-shaped. As a general rule, some ta
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd