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This technique utilizes an enzyme resolvase, endo vii, cloned from the bacteriophage t4. This enzyme has high specificity to find deletions, insertions, and base substitutions mutations or any mismatches. First the pcr is utilized to intensify the normal and mutant allele of targeted sequence, these pcr primers are labeled with fam(6-carboxyfluoroscein amidite) which is a blue fluorescent dye and reverse primer with tet(4,7,2,7-tetra chloro-6-carboxy fluoroscein) amidite which is a green fluorescent dye.
Denaturation and renaturation of normal and mutant allele in a mixture is made, consequently mismatch heteroduplexes will form about 50 % of the time, each and every base pair creates two mismatch. The endo vii enzymes then scan the double stranded dna until it finds structural distortion, either a bubble made by single base pair mutations or a hetero duplex loop created by hybridization of a wild type allele with a mutant allele having an insertion or deletion. The enzyme cleaves with in 6 base pair on 3"side of mutation creating two smaller fragments, one blue and the other one green. The dna sequence is analyzed on automated dna sequence and mobility of every fragment is analyzed.
Spermatophores Many non-chordates do not release free sperms during copulation. They have a mechanism to bundle and enclose a number of sperms in a sheath usually made of gela
Making leaf collections Collect young leaves of as many varieties of plants as possible. Place various layers of news- paper or blotting paper on a firm, smooth board. Next org
Glycerol can act as a substrate for glucose synthesis by conversion to dihydroxyacetone phosphate and an intermediate in gluconeogenesis. In order for citric acid cycle pyruva
OTHE R GLANDS - (i) Mammary glands - Study of mammary gland is mastology. Modified sweat gland. 4-5 pairs in rabbit. 1 pair in human. In opposum 25 pairs (max.).
#question.bacteria and virus are meet so what happen.
Inter-relationships of Classification The aim of classification is to put together organisms on the basis of their similarities. But the question is what type of similarities?
Controlling Introduction of Alien Species As you know the alien species can cause the existing population to decrease or even become extinct. The individuals of alien species
Neurulation in Amphibians The first step in the neurulation process is the flattening and thickening of dorsal ectoderm to form neural plate. The plate of cells are different
Homo neanderthalensis successfully lived in the cold, harsh ice-age conditions of Europe until becoming extinct approximately 30 000 years ago. Adaptations which enabled them to s
The pumps The blood from the operating field is sucked with the help of the cardiotomy sucker and pump, which is filtered to remove all particulate matter and then pass
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