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This technique utilizes an enzyme resolvase, endo vii, cloned from the bacteriophage t4. This enzyme has high specificity to find deletions, insertions, and base substitutions mutations or any mismatches. First the pcr is utilized to intensify the normal and mutant allele of targeted sequence, these pcr primers are labeled with fam(6-carboxyfluoroscein amidite) which is a blue fluorescent dye and reverse primer with tet(4,7,2,7-tetra chloro-6-carboxy fluoroscein) amidite which is a green fluorescent dye.
Denaturation and renaturation of normal and mutant allele in a mixture is made, consequently mismatch heteroduplexes will form about 50 % of the time, each and every base pair creates two mismatch. The endo vii enzymes then scan the double stranded dna until it finds structural distortion, either a bubble made by single base pair mutations or a hetero duplex loop created by hybridization of a wild type allele with a mutant allele having an insertion or deletion. The enzyme cleaves with in 6 base pair on 3"side of mutation creating two smaller fragments, one blue and the other one green. The dna sequence is analyzed on automated dna sequence and mobility of every fragment is analyzed.
Class Bivalvia Body in a bilohed mantle enclosed in a two-valved shell; head reduced; mouth with labial palps but no radula; foot wedge-shaped; plate-like gills; sexes separa
How many cellular nuclei does the pollen tube of angiosperms have? What is the ploidy of each of these nuclei? The pollen tube that is the mature male gametophyte of angiosperm
Homology indicates similarity between the two different nucleotide or the amino acid sequences, generally with potential evolutionary importance. It is probably better to use more
A cell with a euploid number of chromosomes contains: A.one half of a complete set of chromosomes B.several complete sets of chromosomes C.the correct number of chromosomes D.two c
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STRUCTUR E - B R A I N
EFFECT OF ALCOHOL - 1 . EFFECT ON NERVOUS SYSTEM - Alcohol has an anesthetic effect on nervous system. It affects orientation, alertness, coordination, judgement
If the condition or the post disease as a result of intraradicular infection----> treated conventionally via apex " non-surgical ", ---->Here we have 2 options either coronal
Q. What is Colony Collapse Disorder? Bee colonies are attacked by a variety of pathogens and parasites. One major pathogen is the acute bee paralysis virus (IAPV), which cau
Adverse Effects of Valacyclovir Valacyclovir is generally well tolerated; adverse effects are similar to those with acyclovir. GI disturbance, headache, rash, CNS effects such
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