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This technique utilizes an enzyme resolvase, endo vii, cloned from the bacteriophage t4. This enzyme has high specificity to find deletions, insertions, and base substitutions mutations or any mismatches. First the pcr is utilized to intensify the normal and mutant allele of targeted sequence, these pcr primers are labeled with fam(6-carboxyfluoroscein amidite) which is a blue fluorescent dye and reverse primer with tet(4,7,2,7-tetra chloro-6-carboxy fluoroscein) amidite which is a green fluorescent dye.
Denaturation and renaturation of normal and mutant allele in a mixture is made, consequently mismatch heteroduplexes will form about 50 % of the time, each and every base pair creates two mismatch. The endo vii enzymes then scan the double stranded dna until it finds structural distortion, either a bubble made by single base pair mutations or a hetero duplex loop created by hybridization of a wild type allele with a mutant allele having an insertion or deletion. The enzyme cleaves with in 6 base pair on 3"side of mutation creating two smaller fragments, one blue and the other one green. The dna sequence is analyzed on automated dna sequence and mobility of every fragment is analyzed.
Perceptual Disorders The procedure actually constitute a sub-battery and include tests of the subject's ability to recognise shapes by touch and identifies numbers written on t
Define Water Distribution and Compartments of Body Water? Each one of us has a veritable 'sea within', held in place by multiple membranes and our protective envelope of skin.
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The clinical manifestations of IE result from the local destructive effects of intracardiac infection; the embolization of bland or septic fragments of vegetations to
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Biological catalysts à speed up chemical reactions in the body without being consumed in the process Reduce the activation energy needed for reactants to reach the transit
#question.which annelids shws bioluminescence .
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