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This technique utilizes an enzyme resolvase, endo vii, cloned from the bacteriophage t4. This enzyme has high specificity to find deletions, insertions, and base substitutions mutations or any mismatches. First the pcr is utilized to intensify the normal and mutant allele of targeted sequence, these pcr primers are labeled with fam(6-carboxyfluoroscein amidite) which is a blue fluorescent dye and reverse primer with tet(4,7,2,7-tetra chloro-6-carboxy fluoroscein) amidite which is a green fluorescent dye.
Denaturation and renaturation of normal and mutant allele in a mixture is made, consequently mismatch heteroduplexes will form about 50 % of the time, each and every base pair creates two mismatch. The endo vii enzymes then scan the double stranded dna until it finds structural distortion, either a bubble made by single base pair mutations or a hetero duplex loop created by hybridization of a wild type allele with a mutant allele having an insertion or deletion. The enzyme cleaves with in 6 base pair on 3"side of mutation creating two smaller fragments, one blue and the other one green. The dna sequence is analyzed on automated dna sequence and mobility of every fragment is analyzed.
Poverty and Malnutrition Poverty and malnutrition are closely related. As noted before, many elementary aspects of being poor like hunger, inadequate health care, unhygienic l
Define Body Building functions of proteins? The primary functions of proteins, as you might be aware, is tissue growth and maintenance. Protein contains amino acids - the build
Q. What are the vessels that carry blood to the kidneys? Is this blood venous or arterial? The renal arteries are ramifications of the aorta and so the blood filtered by the ki
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What is the Mendel's second law? The Mendel's second law postulates that two or more different traits are also conditioned by two or more pair of different factors that every i
Approach grafting In this method both scion and stock remain rooted. A small slice is cut off from the stem of scion and stock. Scion is bent towards the stock. The two c
Q. Which is the kind of muscle tissue that moves the bones? The bones are moved by the skeletal striated muscles these muscles are voluntary (controlled by volition).
Compare and contrast enantiomers, epimers, and anomers, specifically in relation to sugars.
Q. What is the difference between amnion and chorion? Amnion is the membrane that covers the embryo and Chorion is the membrane that covers the amnion the allantois and the yol
Fungi are a very diverse group of organisms and have a significant impact on the production, spoilage and safety of food. Molds have not only served to synthesize antibiotics but a
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