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This technique utilizes an enzyme resolvase, endo vii, cloned from the bacteriophage t4. This enzyme has high specificity to find deletions, insertions, and base substitutions mutations or any mismatches. First the pcr is utilized to intensify the normal and mutant allele of targeted sequence, these pcr primers are labeled with fam(6-carboxyfluoroscein amidite) which is a blue fluorescent dye and reverse primer with tet(4,7,2,7-tetra chloro-6-carboxy fluoroscein) amidite which is a green fluorescent dye.
Denaturation and renaturation of normal and mutant allele in a mixture is made, consequently mismatch heteroduplexes will form about 50 % of the time, each and every base pair creates two mismatch. The endo vii enzymes then scan the double stranded dna until it finds structural distortion, either a bubble made by single base pair mutations or a hetero duplex loop created by hybridization of a wild type allele with a mutant allele having an insertion or deletion. The enzyme cleaves with in 6 base pair on 3"side of mutation creating two smaller fragments, one blue and the other one green. The dna sequence is analyzed on automated dna sequence and mobility of every fragment is analyzed.
The upper or mountain course - Classification of the river Here the water is fast flowing and runs through a 'V' shaped valley with unstable banks. The fast flowing water has
Q. How can the visual deficiencies known as myopia and hypermetropia be optically explained? Myopia is the visual condition in which the images are formed previous to (in front
Q. What do you know about Tricuspid stenosis? It is rare disease, with rheumatic etiology seen in 90 per cent of cases. In patients with rheumatic mitral stenosis only 3-5 per
Q. What are the nucleic acids? What is the historic origin of this name? RNA and DNA, the nucleic acids, are the molecules responsible for the hereditary information that comma
some deliveries of baby occur early na. y is it so?
Determine the Nature and Degree of Abnormality The nature and degree of abnormality will effect normal neural growth and maturational outcome. For example, in an adult damage t
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From the above reading you must have understood how Dental implants have come into existence. Dental Implants are the future in dentistry and are becoming more and more widely used
Natality Rate - Natality Natality rate or birth rate is determined by dividing the number of individuals born by unit time and is expressed as follows: Natality rate = ΔNn
Pericardial Valves: Carpentier Edwards (Perimount Valve) : Pericardium preserved in gluteraldehyde and mounted on flexible stent is available for mitral and aortic positions
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