Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
This technique utilizes an enzyme resolvase, endo vii, cloned from the bacteriophage t4. This enzyme has high specificity to find deletions, insertions, and base substitutions mutations or any mismatches. First the pcr is utilized to intensify the normal and mutant allele of targeted sequence, these pcr primers are labeled with fam(6-carboxyfluoroscein amidite) which is a blue fluorescent dye and reverse primer with tet(4,7,2,7-tetra chloro-6-carboxy fluoroscein) amidite which is a green fluorescent dye.
Denaturation and renaturation of normal and mutant allele in a mixture is made, consequently mismatch heteroduplexes will form about 50 % of the time, each and every base pair creates two mismatch. The endo vii enzymes then scan the double stranded dna until it finds structural distortion, either a bubble made by single base pair mutations or a hetero duplex loop created by hybridization of a wild type allele with a mutant allele having an insertion or deletion. The enzyme cleaves with in 6 base pair on 3"side of mutation creating two smaller fragments, one blue and the other one green. The dna sequence is analyzed on automated dna sequence and mobility of every fragment is analyzed.
Conscious experience is probably the most familiar mental process that we know, yet its workings remain mysterious. Consciousness is not directly accessible for study. Hence the ne
sample assignment on tagmatizatio
Which are plant tissues that cover the stem and the leaves? The stem perhaps covered by epidermis (having stomata, cuticle and photosynthetic cells) as in monocots or, alternat
Cutaneous larva migrans Cutaneous larva migrans, also known as creeping eruption, creeping verminous dermatitis or serpiginous eruption, is caused by larvae of many nematodes, viz
Explain Hypertension in biochemical or physiological risk factors? The importance of elevated BP as a risk factor for CAD, heart failure, cerebrovascular disease and renal fail
Q. Prevention and Control from escherichia coli? Prevention and Control: Involves avoiding contaminated food and water that have high coliform counts, avoiding unpasteurized ju
What is ISQ In resonance frequency analysis implant stability quotient (ISQ) is use to quantify the frequency measurement of implants within a range between 3500 and 8500 HZ. I
What functions performed by Vitamin and Mineral for athletes? Vitamins and minerals perform the same essential functions for athletes and non-athletes alike. However, owing t
Determine the Horner 's Pupil disease Homer's syndrome occurs because of ipsilateral interruption of the sympathetic outflow to the head and neck. It can result from lesions an
Explain the Transport of Amino Acids? More than one transport or carrier system functions in the absorption of amino acids. The active carrier system for neutral amino acids sh
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd