Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
This technique utilizes an enzyme resolvase, endo vii, cloned from the bacteriophage t4. This enzyme has high specificity to find deletions, insertions, and base substitutions mutations or any mismatches. First the pcr is utilized to intensify the normal and mutant allele of targeted sequence, these pcr primers are labeled with fam(6-carboxyfluoroscein amidite) which is a blue fluorescent dye and reverse primer with tet(4,7,2,7-tetra chloro-6-carboxy fluoroscein) amidite which is a green fluorescent dye.
Denaturation and renaturation of normal and mutant allele in a mixture is made, consequently mismatch heteroduplexes will form about 50 % of the time, each and every base pair creates two mismatch. The endo vii enzymes then scan the double stranded dna until it finds structural distortion, either a bubble made by single base pair mutations or a hetero duplex loop created by hybridization of a wild type allele with a mutant allele having an insertion or deletion. The enzyme cleaves with in 6 base pair on 3"side of mutation creating two smaller fragments, one blue and the other one green. The dna sequence is analyzed on automated dna sequence and mobility of every fragment is analyzed.
Define the Process of Feeding 0-6 Months Infant? The term exclusive breast feeding is used when all fluids, energy and nutrients are provided by breast milk, with the possible
Q. Investigation of Tricuspid stenosis by Echocardiography? Findings are subtle. One should carefully search for organic tricuspid valve involvement in all rheumatic heart dise
Using a Tissue Punch The implant heads are located and using a suitable sized tissue punch(depending on the implant diameter), the tissue punch is used and takes a circular pie
Q. What are the hormones secreted by the thyroid gland? What are their functions? The thyroid secretes the triiodothyronine (T3), hormones thyroxine (T4) and calcitonin. T3
What is thigmotropism? Thigmotropism is the movement or growth of the plant in response to mechanical stimuli (touch or physical contact), as when a plant grows around a suppor
Social Basis of Primitive Life: In the previous section, we gave you a glimpse of how the primitive human beings faced the problems of day to day existence and found some so
Sporogenous Tissue The primary sporogenous cells (PSCs) result after the periclinal division of the archesporial cells. The PSCs may undergo mitotic divisions and increase in
Q. Is the ventricle lumen larger during diastole or during systole? During diastole the opposite occurs. The muscle fibers of the ventricles relax and the lumen of these chambe
Q What is the excretion? Excretion in Physiology is the procedure of elimination of metabolic wastes and other toxic substances from the body.
what is class sarcodina
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd