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This technique utilizes an enzyme resolvase, endo vii, cloned from the bacteriophage t4. This enzyme has high specificity to find deletions, insertions, and base substitutions mutations or any mismatches. First the pcr is utilized to intensify the normal and mutant allele of targeted sequence, these pcr primers are labeled with fam(6-carboxyfluoroscein amidite) which is a blue fluorescent dye and reverse primer with tet(4,7,2,7-tetra chloro-6-carboxy fluoroscein) amidite which is a green fluorescent dye.
Denaturation and renaturation of normal and mutant allele in a mixture is made, consequently mismatch heteroduplexes will form about 50 % of the time, each and every base pair creates two mismatch. The endo vii enzymes then scan the double stranded dna until it finds structural distortion, either a bubble made by single base pair mutations or a hetero duplex loop created by hybridization of a wild type allele with a mutant allele having an insertion or deletion. The enzyme cleaves with in 6 base pair on 3"side of mutation creating two smaller fragments, one blue and the other one green. The dna sequence is analyzed on automated dna sequence and mobility of every fragment is analyzed.
Define effect of Fat on quality and quantity of human milk? Fat content of milk appears to be subject to variability as compared to other constituents. The average fat content
How is the cerebrum anatomically divided? The cerebrum is divided into two cerebral hemispheres, the right and the left. Each hemisphere is made of four cerebral lobes: frontal
OFF PUMP SURGERY : In spite of great advancements in techniques of cardio pulmonary bypass, it is still not physiological. There can be various complications related to perfusion
Im learning about difference cell structures in science. I was wondering if I might be able to get some information about the epithelial tissue, and the connective tissue. Thankyou
Which of the following sugars does NOT contains an O-glycosidic bond? Select one: a. amilose b. amylopectin c. glycogen d. cellulose
Implementation of Nursing Care Provide Bed Rest and Comfort Your major role as a nurse is to provide bed rest to the child and assist the child and parents to understa
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Tunnel Subvalvar Stenosis : This variety is less common and extends from sub aortic area for a variable length of 10-30 mms (tunnel stenosis). In the severe variety, the tun
secondary growth in roots
respiratory system in amphibains
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