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This technique utilizes an enzyme resolvase, endo vii, cloned from the bacteriophage t4. This enzyme has high specificity to find deletions, insertions, and base substitutions mutations or any mismatches. First the pcr is utilized to intensify the normal and mutant allele of targeted sequence, these pcr primers are labeled with fam(6-carboxyfluoroscein amidite) which is a blue fluorescent dye and reverse primer with tet(4,7,2,7-tetra chloro-6-carboxy fluoroscein) amidite which is a green fluorescent dye.
Denaturation and renaturation of normal and mutant allele in a mixture is made, consequently mismatch heteroduplexes will form about 50 % of the time, each and every base pair creates two mismatch. The endo vii enzymes then scan the double stranded dna until it finds structural distortion, either a bubble made by single base pair mutations or a hetero duplex loop created by hybridization of a wild type allele with a mutant allele having an insertion or deletion. The enzyme cleaves with in 6 base pair on 3"side of mutation creating two smaller fragments, one blue and the other one green. The dna sequence is analyzed on automated dna sequence and mobility of every fragment is analyzed.
What is the official name of pyruvic acid? Pyruvic acid is 2-oxopropanoic acid. It is thus a molecule made of three linearly bound carbons with one extremity forming the organi
1.How does the skin on your palm differ from that on the back (posterior) of your hand? 2.Describe the differences observed in the type and distribution of hair on the front (anter
Define Interaction of vitamin c with Ascorbic acid and Leucine? Ascorbic acid : The biologically active form, ascorbate, is a cofactor or co- substrate for eight isolated enz
Q. What is ST Heart Rate Index? To calculate the index, the maximum ST depression is divided by the Delta heart rate (difference between the resting and maximum heart rate). As
PITUITARY DISORDERS - (a) Pituitary Dwarfism . It is caused by the deficiency of growth hormones (GH) from childhood. It is characterised by small but well porportioned body a
Hexokinase, that catalyzes the first irreversible step of glycolysis, is inhibited by glucose 6-phosphate. Thus when PFK is inhibited and fructose 6-phosphate builds up an
if i start in the superior mesenteric vain where do i have to go through to get to the left anterior circumflex humeral artery. by the way of the hepatic portal vein and the heart
Things to do- We ought to aide by earthquake resisting building codes during construction and retrofitting of existing buildings. We should have a first AID box handy and learn
poor metabolism phenotype will have
Diabetes mellitm This anabolic hormone exerts its action on key glycolytic enzymes thus leading to the conversion of glucose to pyruvate as explained under the regulation
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