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This technique utilizes an enzyme resolvase, endo vii, cloned from the bacteriophage t4. This enzyme has high specificity to find deletions, insertions, and base substitutions mutations or any mismatches. First the pcr is utilized to intensify the normal and mutant allele of targeted sequence, these pcr primers are labeled with fam(6-carboxyfluoroscein amidite) which is a blue fluorescent dye and reverse primer with tet(4,7,2,7-tetra chloro-6-carboxy fluoroscein) amidite which is a green fluorescent dye.
Denaturation and renaturation of normal and mutant allele in a mixture is made, consequently mismatch heteroduplexes will form about 50 % of the time, each and every base pair creates two mismatch. The endo vii enzymes then scan the double stranded dna until it finds structural distortion, either a bubble made by single base pair mutations or a hetero duplex loop created by hybridization of a wild type allele with a mutant allele having an insertion or deletion. The enzyme cleaves with in 6 base pair on 3"side of mutation creating two smaller fragments, one blue and the other one green. The dna sequence is analyzed on automated dna sequence and mobility of every fragment is analyzed.
Q. How do antibodies work to neutralize antigens? The immunoglobulins or antibodies act to facilitate the destruction of antigens: they attract phagocytic leukocytes, they dire
Glycogenolysis Unlike glycogenesis, glycogenolysis is the breakdown of glycogen. Glycogen is broken down in the liver and muscle catalysed by the enzyme glycogen phosph
An electronic chip is to be implanted in the body. During in vitro (in the lab) testing it is observed that the chip will dissolve over time if exposed to liquid with similar pH to
Explain in 100+ words Why/how did molecular biology change forsenic biology.
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Residue is defined medically as the solid contents that have reached the lower intestine. A low residue diet is composed of foods, which are easily digested and readily absorbed, r
1. FIBROU S OR IMMOVABLE JOINTS - Occur between the bones of cranium & in the tooth suckets commonly in the form of sutures. 2 . CARTILAGINOUR OR SLIGHTLY MOVAB
What is Specific defence mechanism Specific: These relate to specialized cells located throughout the body which respond to invasion of foreign materials/microorganisms su
Manx cats are heterozygous for a dominant mutation that results in no tails or very short tails, large hind legs and a distinctive gait. The mating of two Manx cats yields two Manx
Composition - Culture Medium A standard plant tissue culture medium (Basal medium) contains all the essential macro elements (Carbon, hydrogen, oxygen nitrogen, phosphorus, su
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