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This technique utilizes an enzyme resolvase, endo vii, cloned from the bacteriophage t4. This enzyme has high specificity to find deletions, insertions, and base substitutions mutations or any mismatches. First the pcr is utilized to intensify the normal and mutant allele of targeted sequence, these pcr primers are labeled with fam(6-carboxyfluoroscein amidite) which is a blue fluorescent dye and reverse primer with tet(4,7,2,7-tetra chloro-6-carboxy fluoroscein) amidite which is a green fluorescent dye.
Denaturation and renaturation of normal and mutant allele in a mixture is made, consequently mismatch heteroduplexes will form about 50 % of the time, each and every base pair creates two mismatch. The endo vii enzymes then scan the double stranded dna until it finds structural distortion, either a bubble made by single base pair mutations or a hetero duplex loop created by hybridization of a wild type allele with a mutant allele having an insertion or deletion. The enzyme cleaves with in 6 base pair on 3"side of mutation creating two smaller fragments, one blue and the other one green. The dna sequence is analyzed on automated dna sequence and mobility of every fragment is analyzed.
Restaurant deep fat frying evaluation A number of factors are studied when evaluating frying oils. During deep fat frying, the fat is exposed continuously to elevated temperat
Hybridoma is the clone of plasmacytoma cells which secrete a monoclonal antibody; commonly produced by the fusion of peripheral or splenic plasma cells taken from the immunized mo
Q. Explain Femoral Approach? This approach involves the insertion of a catheter over a guidewire i.e. inserted into a sheath in the right femoral artery. Systemic anticoagulati
To which direction does the growth of one side of a stem, branch or root induce the structure to curve? While one side of a stem, branch or root grows more than the other side
A charged particle A exerts a force of 2.39uN to the right on charged particle B when the particles are 12.3 mm apart. Particle B moves straight away from A to make the distance be
reproductive system of rabbit
please enlighten me on proteins and forensics
TEMPERATURE Temperature is a major physical environmental factor which profoundly influences the vital activities of living organisms like, metabolism, growth and reproduction.
What is Passive Transport? Passive Transport : Specialized proteins called permeases also move specific substances across the cell membrane in a process called carrier transp
Q. Define Low - density lipoprotein? Ans. LDL is the major cholesterol-rich lipoprotein carrying approximately 70 per cent of plasma cholesterol. It serves to transport ch
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