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This technique utilizes an enzyme resolvase, endo vii, cloned from the bacteriophage t4. This enzyme has high specificity to find deletions, insertions, and base substitutions mutations or any mismatches. First the pcr is utilized to intensify the normal and mutant allele of targeted sequence, these pcr primers are labeled with fam(6-carboxyfluoroscein amidite) which is a blue fluorescent dye and reverse primer with tet(4,7,2,7-tetra chloro-6-carboxy fluoroscein) amidite which is a green fluorescent dye.
Denaturation and renaturation of normal and mutant allele in a mixture is made, consequently mismatch heteroduplexes will form about 50 % of the time, each and every base pair creates two mismatch. The endo vii enzymes then scan the double stranded dna until it finds structural distortion, either a bubble made by single base pair mutations or a hetero duplex loop created by hybridization of a wild type allele with a mutant allele having an insertion or deletion. The enzyme cleaves with in 6 base pair on 3"side of mutation creating two smaller fragments, one blue and the other one green. The dna sequence is analyzed on automated dna sequence and mobility of every fragment is analyzed.
Define risk of stress fracture among Athletes? There is an increased risk of stress fracture among athletes which is associated with lower calcium intake and lower bone density
Pyruvate kinase catalyzes the third irreversible move in glycolysis. It is activated by fructose 1, 6-bisphosphate. The ATP and amino acid alanine allosterically inhibit the enzy
write the charecteristics of lake ecosystem
Ernst Mayr, for whom, species and species problem have been the main concern of research, points out that speciation, the multiplication of species, that is division of one parent
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Define the Clinical success for the Root Canal Treatment a) Absence of pain and swelling. b) Disappearance of sinus tract. c) No evidence of soft tissue destruction, incl
Infective Endocarditis It is a term used to include subacute bacterial endocarditis as well as infection caused by viruses and fungi. The causative organisms responsibl
What is Lactate Lactate is transported to the liver in the Cori cycle (lactic acid cycle) and is converted to pyruvate. Hepatic gluconeogenesis then converts lactate ba
A woman who is heterozygous for a particular X-linked recessive trait marries a phenotypically normal man. What percentage of their sons will show the recessive phenotype?
Biodiversity provides us with life -sustaining services. As discussed below, these ecosystem services have a much higher value than most people realize. At the ecosystem level, bio
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