Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
This technique utilizes an enzyme resolvase, endo vii, cloned from the bacteriophage t4. This enzyme has high specificity to find deletions, insertions, and base substitutions mutations or any mismatches. First the pcr is utilized to intensify the normal and mutant allele of targeted sequence, these pcr primers are labeled with fam(6-carboxyfluoroscein amidite) which is a blue fluorescent dye and reverse primer with tet(4,7,2,7-tetra chloro-6-carboxy fluoroscein) amidite which is a green fluorescent dye.
Denaturation and renaturation of normal and mutant allele in a mixture is made, consequently mismatch heteroduplexes will form about 50 % of the time, each and every base pair creates two mismatch. The endo vii enzymes then scan the double stranded dna until it finds structural distortion, either a bubble made by single base pair mutations or a hetero duplex loop created by hybridization of a wild type allele with a mutant allele having an insertion or deletion. The enzyme cleaves with in 6 base pair on 3"side of mutation creating two smaller fragments, one blue and the other one green. The dna sequence is analyzed on automated dna sequence and mobility of every fragment is analyzed.
Haptotaxis - Modes of Cell Movement Haptotaxis considers to the directed movement of cells in response to a concentration gradient of an adhesive molecule that might be presen
Illustrate the metabolism of cornea? Metabolism of Cornea: The corneal epithelium plays several roles in the process of image formation. Its apical cell border interacts
Surgical significance of Inferior alveolar nerve and artery In an excessively resorbed ridge, the mental foramen with its contents of mental nerve and vessels, can be found o
about kinematics
After ovulation:- (a) what structure replaces the Graafian follicle (b) what hormone does it produce? (a) After ovulation, the follicle is replaced by the cor
Why can the consumption of molecular oxygen indicate the metabolic rate of aerobic organisms? Molecular oxygen (O2) consumption has direct relation to the cell metabolic rate i
what are inorganic species
Why do ecological models commonly have limited applications? The applications of ecological models are limited due to the models are normally very simple and do not accurately
Q. Which organs of the body is part of the human digestive system? The digestive system also known as "systema digestorium" or gastrointestinal system is composed of the digest
Zygotic or Suspensor Polyembryony Cleavage of the apical cells of the globular or filamentous proembryo produced by the zygote may result in two or more embryos in a seed. Th
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd