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This technique utilizes an enzyme resolvase, endo vii, cloned from the bacteriophage t4. This enzyme has high specificity to find deletions, insertions, and base substitutions mutations or any mismatches. First the pcr is utilized to intensify the normal and mutant allele of targeted sequence, these pcr primers are labeled with fam(6-carboxyfluoroscein amidite) which is a blue fluorescent dye and reverse primer with tet(4,7,2,7-tetra chloro-6-carboxy fluoroscein) amidite which is a green fluorescent dye.
Denaturation and renaturation of normal and mutant allele in a mixture is made, consequently mismatch heteroduplexes will form about 50 % of the time, each and every base pair creates two mismatch. The endo vii enzymes then scan the double stranded dna until it finds structural distortion, either a bubble made by single base pair mutations or a hetero duplex loop created by hybridization of a wild type allele with a mutant allele having an insertion or deletion. The enzyme cleaves with in 6 base pair on 3"side of mutation creating two smaller fragments, one blue and the other one green. The dna sequence is analyzed on automated dna sequence and mobility of every fragment is analyzed.
Congenital Defects Related to Kidney/Upper Urinary Tract i) Renal Agenesis A new born baby with this condition may show low set ears, flat nose, prominent epicanthic
Kreb's cycle is the biochemical cycle in the cellular aerobic metabolism where the acetyl CoA is combined with the oxaloacetate to form citric acid; the resulting citric acid is c
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Mentalis The mental tubercles on either side of mental protruberance(in midline) gives origin to the mentalis muscle. Above the mentalis origin, the incisivus muscle takes orig
Your breakfast consists of a cup of black coffee with sugar as well as a plain bagel covered with cream cheese. Describe the digestion of this breakfast as it passes through each m
Explain about the civil law. Civil law: This region of the law explains the rights and obligations of persons. This resolves any disputes occurring between persons over t
What are cotyledons? Cotyledons or else seed leaves are structures formed by the embryo of angiosperms to absorb nutrients from the endosperm and to store and transfer these nu
The adaptive mechanisms may be short term ones which come into play within minutes or hours of the onset of myocardial dysfunction. These are: Frank-Starling Mechanism In
Q. How are the male gametes of gymnosperms formed? What is the relationship between the pollen grains and the concept of alternation of generations? In the male strobiles (cone
Viscosity and Plasticity of colloidal particle Various degrees of viscosity and plasticity are encountered in colloids. Viscosity may be described as resistance to pouring.
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