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This technique utilizes an enzyme resolvase, endo vii, cloned from the bacteriophage t4. This enzyme has high specificity to find deletions, insertions, and base substitutions mutations or any mismatches. First the pcr is utilized to intensify the normal and mutant allele of targeted sequence, these pcr primers are labeled with fam(6-carboxyfluoroscein amidite) which is a blue fluorescent dye and reverse primer with tet(4,7,2,7-tetra chloro-6-carboxy fluoroscein) amidite which is a green fluorescent dye.
Denaturation and renaturation of normal and mutant allele in a mixture is made, consequently mismatch heteroduplexes will form about 50 % of the time, each and every base pair creates two mismatch. The endo vii enzymes then scan the double stranded dna until it finds structural distortion, either a bubble made by single base pair mutations or a hetero duplex loop created by hybridization of a wild type allele with a mutant allele having an insertion or deletion. The enzyme cleaves with in 6 base pair on 3"side of mutation creating two smaller fragments, one blue and the other one green. The dna sequence is analyzed on automated dna sequence and mobility of every fragment is analyzed.
Pathophysiology Insufficient amount of iron present in the body leads to reduction in serum transferrin (Serum betaglobulin, which binds and transports iron) saturation. T
Homologous structures are the body parts in different organisms which have similar bones and similar arrangements of the blood vessels, muscles, and nerves and go through similar
Explain what is Genetic Factors ? It has been suggested that certain genetic defect(s) could be partly responsible for increased risk of CAD in Asian Indians. No specific genet
Helix-turn-helix This motif have of two -helices divided by a short (four-amino acid) peptide sequence which forms a -turn that shown in above figure. When
How many kidney in man?
Determine some Food Sources of chromium? Chromium occurs in trivalent form in foods. Good sources of chromium include whole grains, spices and condiments, meats especially orga
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Q. Where do the photochemical and the chemical phase of photosynthesis occur? The photochemical phase of the photosynthesis procedure occurs mainly on the thylakoids the green
How to Defibrillate? Three shocks should be given initially in a 200J - 200J - 360J sequence. For most recoverable situations, 200J would be adequate and results in least myco
If a person is heterozygous for the 32 allele of the CCR5 gene, how many of the four daughter cells produced by meiosis will have the 32 allele?
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