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This technique utilizes an enzyme resolvase, endo vii, cloned from the bacteriophage t4. This enzyme has high specificity to find deletions, insertions, and base substitutions mutations or any mismatches. First the pcr is utilized to intensify the normal and mutant allele of targeted sequence, these pcr primers are labeled with fam(6-carboxyfluoroscein amidite) which is a blue fluorescent dye and reverse primer with tet(4,7,2,7-tetra chloro-6-carboxy fluoroscein) amidite which is a green fluorescent dye.
Denaturation and renaturation of normal and mutant allele in a mixture is made, consequently mismatch heteroduplexes will form about 50 % of the time, each and every base pair creates two mismatch. The endo vii enzymes then scan the double stranded dna until it finds structural distortion, either a bubble made by single base pair mutations or a hetero duplex loop created by hybridization of a wild type allele with a mutant allele having an insertion or deletion. The enzyme cleaves with in 6 base pair on 3"side of mutation creating two smaller fragments, one blue and the other one green. The dna sequence is analyzed on automated dna sequence and mobility of every fragment is analyzed.
EXCRETION IN PRAWN- A pair of antennary gland or green gland and a median renal sac present. Green glands lie at the base of antennae & open out by renal aperture.
Explain biomaterial implantation When a biomaterial is introduced into the host, in addition to a host response there is also a material response. Immediately on implantation,
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What are the major natural plant hormones and what are their respective effects? The major natural plant hormones and their respective functions are the following- Auxins that
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Food require during thyphoid So remember the foods to be included are: Juices, soups, dal water, broths. Refined cereals and their products (e.g. maida, rava, bread, rice
How is it structurally explained that the motor activity of the left side of the body is controlled by the right cerebral hemisphere and the motor activity of the right side of the
Q. What are the few examples of the energetic function of organic molecules? Since they are complex molecules, organic molecules store large amount of energy, presenting many c
While waiting at an airport , nail Campbell once overheard this claim, its paranoid and ignorant to worry about industry or agriculture contaminating the environment with chemical
Medical Nutrition Therapy Medical Nutrition Therapy (MNT) is defined as the assessment of the nutritional status of a client followed by nutrition therapy ranging Pon
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