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This technique utilizes an enzyme resolvase, endo vii, cloned from the bacteriophage t4. This enzyme has high specificity to find deletions, insertions, and base substitutions mutations or any mismatches. First the pcr is utilized to intensify the normal and mutant allele of targeted sequence, these pcr primers are labeled with fam(6-carboxyfluoroscein amidite) which is a blue fluorescent dye and reverse primer with tet(4,7,2,7-tetra chloro-6-carboxy fluoroscein) amidite which is a green fluorescent dye.
Denaturation and renaturation of normal and mutant allele in a mixture is made, consequently mismatch heteroduplexes will form about 50 % of the time, each and every base pair creates two mismatch. The endo vii enzymes then scan the double stranded dna until it finds structural distortion, either a bubble made by single base pair mutations or a hetero duplex loop created by hybridization of a wild type allele with a mutant allele having an insertion or deletion. The enzyme cleaves with in 6 base pair on 3"side of mutation creating two smaller fragments, one blue and the other one green. The dna sequence is analyzed on automated dna sequence and mobility of every fragment is analyzed.
Why do substances react with each other? Chemical reactions occur due to the products of the reaction have less energy than the reactants (drive toward less energy). These reac
Define Abbe Condenser of Microscope? It is present beneath the stage, as shown in Figure. It collects and focuses a cone of light on the slide. Its position can be adjusted ver
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Give three examples of artificial selection. Include examples of both animals and plants. Answers will vary, but could include domestic cats, domestic dogs, cattle, sheep, an
What is the formula for calculation of bone distortion The following formula can be used to calculate for distortion: Effective Bone available = Reference sphere diameter ×
Determine the term - Akinetic and Myoclonic Seizures Akinetic seizures are ordinarily seen only in children. Usually, an affected child collapses suddenly and without warning.
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