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This technique utilizes an enzyme resolvase, endo vii, cloned from the bacteriophage t4. This enzyme has high specificity to find deletions, insertions, and base substitutions mutations or any mismatches. First the pcr is utilized to intensify the normal and mutant allele of targeted sequence, these pcr primers are labeled with fam(6-carboxyfluoroscein amidite) which is a blue fluorescent dye and reverse primer with tet(4,7,2,7-tetra chloro-6-carboxy fluoroscein) amidite which is a green fluorescent dye.
Denaturation and renaturation of normal and mutant allele in a mixture is made, consequently mismatch heteroduplexes will form about 50 % of the time, each and every base pair creates two mismatch. The endo vii enzymes then scan the double stranded dna until it finds structural distortion, either a bubble made by single base pair mutations or a hetero duplex loop created by hybridization of a wild type allele with a mutant allele having an insertion or deletion. The enzyme cleaves with in 6 base pair on 3"side of mutation creating two smaller fragments, one blue and the other one green. The dna sequence is analyzed on automated dna sequence and mobility of every fragment is analyzed.
Explain Operational Taxonomic Units - Numerical Taxonomy 1) Then the characters to be used for studying the OTUs are selected. Usually a large number of characters are taken as
Food Pyramid The diabetes food pyramid divides food into six groups. These groups vary with each other on the basis of size occupied in the pyramid. The largest i.e. grains, br
What is anemia? What are the four main types of anemia? Anemia is low concentration of hemoglobin in the blood. The four main types of anemia are the nutrient-deficiency ane
define alimentary canal
PSYCHOLOGICAL DISORDERS - 1 . Anxiety Disorders 2 . Obsessive compulsive disorders 3. Attention deficit disorder 4 . Mood disorders
1.sarcomastigophora 2.sporozoa 3.cnidospora 4.ciliophora
Molluscus - Hormones in Growth and Reproduction We know quite well about the endocrine mechanism of growth and reproduction in the freshwater snail Lymnaea stagnalis. This is
Q. (a) In droplet infection (i) where do the droplets come from, (ii) what infective agents might they contain? (b) Give two examples of diseases normally spread
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What are vitamins? What are the main vitamins needed by humans? Most vitamins are coenzymes (fundamental substances for the enzyme functioning) that are not formed by the organ
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