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This technique utilizes an enzyme resolvase, endo vii, cloned from the bacteriophage t4. This enzyme has high specificity to find deletions, insertions, and base substitutions mutations or any mismatches. First the pcr is utilized to intensify the normal and mutant allele of targeted sequence, these pcr primers are labeled with fam(6-carboxyfluoroscein amidite) which is a blue fluorescent dye and reverse primer with tet(4,7,2,7-tetra chloro-6-carboxy fluoroscein) amidite which is a green fluorescent dye.
Denaturation and renaturation of normal and mutant allele in a mixture is made, consequently mismatch heteroduplexes will form about 50 % of the time, each and every base pair creates two mismatch. The endo vii enzymes then scan the double stranded dna until it finds structural distortion, either a bubble made by single base pair mutations or a hetero duplex loop created by hybridization of a wild type allele with a mutant allele having an insertion or deletion. The enzyme cleaves with in 6 base pair on 3"side of mutation creating two smaller fragments, one blue and the other one green. The dna sequence is analyzed on automated dna sequence and mobility of every fragment is analyzed.
What is Implant Failure The total failure of the implant to fulfill its purpose which are functional, esthetic and phonetic because of mechanical or biologic reasons.
Chromium Chromium functions as a component of the glucose tolerance factor, which serves to potentiate the action of insulin. Adding low concentrations (0.02 to 1.0 mg/kg) of c
How does biological diversity relate to the characteristics of the abiotic factors of an ecosystem? The availability of abiotic factors as light, moisture, mineral salts, heat
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Which is the part of the human visual system where the receptors that sense light, i.e., the photoreceptor cells, are located? How do those cells work? The photoreceptor cells
Insulin Pump An insulin pump is a device used for the administration of insulin, also known as continuous subcutaneous insulin infusion therapy. The device includes: - the
Q. What do you mean by Canning? Canning The term canning is generally applied to foods, more specifically, to the foods preserved by heat processing. It aimgto destroy microo
what cell organelle does photosynthesis take place in?
SPERM A T OCY T OGENESIS In this process four spermatid develop from one PGC. (i) Multiplication phase The spermotogonia or sperm mother cells lie next to the b
Which are the types of neurons that participate in the spinal arch reflex? Where are their cell bodies situated? In the arch reflex first a sensory neuron located in the gangli
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