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This technique utilizes an enzyme resolvase, endo vii, cloned from the bacteriophage t4. This enzyme has high specificity to find deletions, insertions, and base substitutions mutations or any mismatches. First the pcr is utilized to intensify the normal and mutant allele of targeted sequence, these pcr primers are labeled with fam(6-carboxyfluoroscein amidite) which is a blue fluorescent dye and reverse primer with tet(4,7,2,7-tetra chloro-6-carboxy fluoroscein) amidite which is a green fluorescent dye.
Denaturation and renaturation of normal and mutant allele in a mixture is made, consequently mismatch heteroduplexes will form about 50 % of the time, each and every base pair creates two mismatch. The endo vii enzymes then scan the double stranded dna until it finds structural distortion, either a bubble made by single base pair mutations or a hetero duplex loop created by hybridization of a wild type allele with a mutant allele having an insertion or deletion. The enzyme cleaves with in 6 base pair on 3"side of mutation creating two smaller fragments, one blue and the other one green. The dna sequence is analyzed on automated dna sequence and mobility of every fragment is analyzed.
Adverse effects of menactra The most common adverse reactions to Menactra include headache, fatigue and malaise, in addition to pain, redness and induration at the site of injec
Define Harvard Step Test - aerobic capacity? In this test, the subject steps on and off an 18-inch platform at a rate of 30 times per minute. The evaluator records the subject'
OXIDATION OF PYRUVATE TO ACETYL CoA The oxidation of pyruvate to acetyl CoA is the irreversible route from glycolysis to the citric acid cycle. What is citric acid cycle?
During Abutment Connection Implants should be tested for the presence of any mobility and the radiographs should be taken. These radiographs are very vital as they serve as the
Q. Where are the adrenal glands located? How many are they and what are their portions? Each adrenal gland is located on the top of each kidney (forming a hat-like structure fo
Q. What is the nitrogen waste in amphibian larvae and in the adult animal? The terrestrial adult excretes urea since amphibian larvae are aquatic they excrete ammonia.
Q. Causes of Malnutrition in Inflammatory Bowel Disease? The causes of malnutrition include: • Decreased oral intake, which can be disease induced due to abdominal pain, dia
what are the economic importance of obelia?
what is respiration of plants and its types
Explain anterior pituitary gland secretes hormones The anterior pituitary gland secretes hormones that elevate the blood glucose and therefore antagonize the action of glucose
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