Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
This technique utilizes an enzyme resolvase, endo vii, cloned from the bacteriophage t4. This enzyme has high specificity to find deletions, insertions, and base substitutions mutations or any mismatches. First the pcr is utilized to intensify the normal and mutant allele of targeted sequence, these pcr primers are labeled with fam(6-carboxyfluoroscein amidite) which is a blue fluorescent dye and reverse primer with tet(4,7,2,7-tetra chloro-6-carboxy fluoroscein) amidite which is a green fluorescent dye.
Denaturation and renaturation of normal and mutant allele in a mixture is made, consequently mismatch heteroduplexes will form about 50 % of the time, each and every base pair creates two mismatch. The endo vii enzymes then scan the double stranded dna until it finds structural distortion, either a bubble made by single base pair mutations or a hetero duplex loop created by hybridization of a wild type allele with a mutant allele having an insertion or deletion. The enzyme cleaves with in 6 base pair on 3"side of mutation creating two smaller fragments, one blue and the other one green. The dna sequence is analyzed on automated dna sequence and mobility of every fragment is analyzed.
What are the main events of the final mitotic period? The final mitotic phase is telophase. In telophase the following events happens: decondensation of chromosomes, every set
Q. Global alteration of brain functioning - Sleep? Sleep involves a global alteration of brain functioning, and occupies one third of our lives, The transition from waking to
Define functions of carbohydrate - Protein sparing effect? Carbohydrates help in regulating the protein metabolism. Presence of sufficient carbohydrates to meet energy demands
Q. Show the Valuation of Biodiversity? Serious research in this field has only been recently initiated and the methodologies for valuation are still evolving. Important valuat
Explain how the uterus supports the development of a baby during gestation
Why do some physicians advise patients with a bladder infection to drink a lot of cranberry juice? Answer Cranberry juice contains chemicals that kill viruses and bacteria. The inc
Asexual Reproduction - Its Prevalence and Significance Having studied the several aspects of asexual reproduction in the non-chordates we can now make a few generalizations.
What is the difference between homologous and heterologous immunoglobulins? Homologous immunoglobulin is the human (from the similar species) immunoglobulin. In case of inocula
Define Supplementation to prevention of iron deficiency anaemia? Supplementation with low doses of iron is necessary to prevent anaemia in particular groups of people. Fortifie
Nutrient Cycles All ecosystems have certain common basic features of structure and function. They all have living and non-living components through which there is a flow of en
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd