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This technique utilizes an enzyme resolvase, endo vii, cloned from the bacteriophage t4. This enzyme has high specificity to find deletions, insertions, and base substitutions mutations or any mismatches. First the pcr is utilized to intensify the normal and mutant allele of targeted sequence, these pcr primers are labeled with fam(6-carboxyfluoroscein amidite) which is a blue fluorescent dye and reverse primer with tet(4,7,2,7-tetra chloro-6-carboxy fluoroscein) amidite which is a green fluorescent dye.
Denaturation and renaturation of normal and mutant allele in a mixture is made, consequently mismatch heteroduplexes will form about 50 % of the time, each and every base pair creates two mismatch. The endo vii enzymes then scan the double stranded dna until it finds structural distortion, either a bubble made by single base pair mutations or a hetero duplex loop created by hybridization of a wild type allele with a mutant allele having an insertion or deletion. The enzyme cleaves with in 6 base pair on 3"side of mutation creating two smaller fragments, one blue and the other one green. The dna sequence is analyzed on automated dna sequence and mobility of every fragment is analyzed.
Diestrus- Estrous cycle This lasts 60 to 70 hours during which functional regression of the corpora lutea occurs. The uteri are small, anaemic, and only slightly contractile.
What is Smooth Muscle? Smooth muscle provides the contractile force for movement in internal organs under control of the involuntary or autonomic nervous system. Smooth muscle
what is the name of the valve that separates the chamber?Why is that valve necessary?
THORACI C CAVITY - Thoracic cavity is air tight. On dorsal side vertebral column present. On ventral side sternum present. On lateral side 12 pairs ribs present. On p
life cycle of stentor
What proportion of children with Down syndrome do you expect when women with down syndrome have children with men who have 46 chromosomes? Justify answer
Absence of Passive Fit of the Prosthesis A passive fit of the prosthesis reduces long term stresses in the superstructure, implant components and the bone adjacent to the impla
What is the function, other than protection, of the ribs? The ribs help to alter the volume of the thorax during breathing movements.
How to draw diagrams of bones
Q. Flavour of food product? Flavour factors include both sensations perceived by the tongue, which include sweet, salty, sour and bitter tastes and aromas perceived by the tong
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