Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Baculovirus is widely used for expressing recombinant proteins. Structural of baculovirus is a rod-shaped nucleocapsid containing genomic DNA. The baculovirus Autographa California multiple nuclear polyhedrosis virus (AcMNPV) used as a vector in many gene therapies studies in the science world. The wild-type AcMNPV does not replicate in mammalian cells but it can transfer then. Due to higher level of gene expression, the baculovirus are active. In insect cell culture it easy to maintain the culture compared to mammalian cells. The infection of Baculovirus can turn off host cell protein expression and impair secretion apparatus of Endoplasmic reticulum (ER)-Golgi pathway proteins. But some strategies have been developed to improve secretion protein. Chaperones proteins are potential on making secrete protein production up to two fold. Secretory alkaline phosphate-EGFP fusion protein (SEFP) as co-expressed with chaperones, calreticulin (CALR) which located in storage compartment associated with ER. Alpha Synuclein (α-syn) and Beta Synuclein (β-syn) is a small protein, α-syn work as a small heat shock protein. Both of them are molecular chaperones.
The aim of this project is to enhance the expression of the SEFP testing chaperones (α-syn, β-syn with Calreticulin as a positive control, and dsRed as a negative control) which may increase and enabled correct folding protein forms. We will test promoters on virus expression levels: gp64 to use if timing of promoter expression/protein production is crucial to expression levels. -------- > more explanation about gp64! How it works?
In order for making Baculovirus based on AcMNPV this project will use Flash BacUltra. It is a new platform technology for the production of recombinant baculoviruses, optimised for producing 100 % recombinant virus by eliminating the need for plaque purification and it also increases protein yield.
Q. What is secondary structure of a protein? The secondary protein structure is creating by the manner its amino acids interact through the intermolecular bond. These communica
Q. Show Nutrition guidelines for prevention of heart disease? Nutrition guidelines for prevention of heart disease as suggested by WHO are highlighted. Details regardin
what year did virchow state where a cell exists there must have been a pre-existing cell
Express the term in brief - Protonephridia. An osmoregulatory-excretory structure found in some invertebrates. Also known as a flamecell, this tubule is closed at its distal en
Freediving is an activity in which a person dives, sometimes to great depth, without the use of scuba gear. The diver must hold his or her breath for the duration of the dive. (The
What is genotype? What is the difference between phenotype and genotype? The Genotype is the genes, DNA nucleotide sequences contained in the chromosomes of an individual, whic
Metabolic Process Location Within the study of biochemistry, what tends to obtain lost amidst the numerous pathways and enzymes is the fact which metabolism concerns the overal
Define the types of Ketogenic Diets? The ketogenic diet is initiated after an initial period of Pasting for 24-72 hours, till ketosis is established. There are two types of ket
Characteristics of Thin Gingiva Highly scalloped soft tissue and bony architecture Delicate friable soft tissue Minimal amount of attached gingiva Thin underlying
Valuation of biological resources includes the process of deriving a monetary value to the things that are not sold in the market. Examples are the value given to fuelwood gathere
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +1-415-670-9521
Phone: +1-415-670-9521
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd