Embryonic stem cells (es), Biology

Assignment Help:

Embryonic stem cells (ES): These are pleuripotent cells of early embryo (blastocyst) capable of giving rise to all differentiated cells, including germ line cells. Embryonic stem cells (ES) can be cultured maintained and are able to proliferate. The method of producing transgenic mice by genetically modified embryonic stem cells is as follows:

(i)  Embryonic stem cell (ES) culture is initiated.

(ii)  The ES are transformed and screening is done in PCR analysis.

(iii)  Transformed cells can be cultured and inserted into blastocyst, which are then implanted in foster mother.

(iv)  Transgenic lines are determined by crosses from founder mice.


Related Discussions:- Embryonic stem cells (es)

Explain requirements and recommended dietary intake, Explain Requirements a...

Explain Requirements and Recommended Dietary Intake? A very small daily intake of vitamin C (10-15 mg/day for an adult) is required to avoid deficiency and stave off scurvy. Ho

Determine the fructose to glucose ratio in honey, Q. Determine the fructose...

Q. Determine the fructose to glucose ratio in honey? This activity will help you to: • check the given samples of honey for any adulteration with commercial sugar

Define minerals, Define minerals Definition of minerals, as it applies ...

Define minerals Definition of minerals, as it applies to food and nutrition usually refers to  the elements other than carbon, hydrogen, oxygen and nitrogen that are present in

Isolate genomic dna, You may have heard in the news currently that a new sp...

You may have heard in the news currently that a new species of frog was discovered within a rainforest. You objective are to isolate genomic DNA and construct an expression libr

Explain diseases of pericardium, Q. Explain Diseases of pericardium? Pe...

Q. Explain Diseases of pericardium? Pericardium is the sac covering the heart. Pericardium consists of two layers-the visceral pericardium (epicardium) and the parietal pericar

Neurons, how does sodium and potassium ions maintain the resting petential ...

how does sodium and potassium ions maintain the resting petential of neurons

Define about anthropometric and physiological, Define about Anthropometric ...

Define about Anthropometric and Physiological? Various physiological and anthropometric measurements give us an indication of the present status of an individual based on which

Transmission electron microscopy, Transmission Electron Microscopy The so...

Transmission Electron Microscopy The source of illumination in a TEM is a tungsten filament that emits electrons. A vacuum is created to prevent the collision of electrons with a

Metabolism of carbohydrates, Carbohydrates form the major bulk of human die...

Carbohydrates form the major bulk of human diet and are also the chief sources of energy. Carbohydrates are easily digested and broken down by enzyme action into glucose and are ea

Explain about the anti obesity drugs, Explain about the Anti Obesity Drugs?...

Explain about the Anti Obesity Drugs? The anti-obesity drugs can be classified into two broad groups as indicated in the Figure. Drugs must be taken only under Doctor's

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd