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Embryonic stem cells (ES): These are pleuripotent cells of early embryo (blastocyst) capable of giving rise to all differentiated cells, including germ line cells. Embryonic stem cells (ES) can be cultured maintained and are able to proliferate. The method of producing transgenic mice by genetically modified embryonic stem cells is as follows:
(i) Embryonic stem cell (ES) culture is initiated.
(ii) The ES are transformed and screening is done in PCR analysis.
(iii) Transformed cells can be cultured and inserted into blastocyst, which are then implanted in foster mother.
(iv) Transgenic lines are determined by crosses from founder mice.
Vital Dye Staining In this type of method, a piece of agar or cellophane soaked in the solution of a vital dye (Neutral Red, Nile Blue Sulphate, and Bismarck Brown etc.) is a
Acid butt - carbohydrate utilization pattern test ? Acid butt (yellow) and acid slant (yellow) with or without gas production – Glucose along with lactose and/or sucrose, is deg
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Class of Mollusca - Monoplacophora Bilaterally symmetrical, with broad flat foot and single shell, mantle cavity has five to six pairs of gills; six pairs of nephridia of whi
genral charecter and classificatin of porifera
Ask question #M1. Discuss why Obelia is considered to be of special interest in Zoology as an animal showing an intermediate grade of organization. inimum 100 words accepted#
Determine the prosthetic planning Complete prosthetic planning has to be done and the implants planned accordingly. Thereafter the surgical phase should be carried out. Once th
Q. What is hypertension? The Hypertension is a disease in which the arterial blood pressure, during systole or during diastole, is abnormally high. The Hypertension, or high
Dideoxy Sequencing is the enzymatic determination and consideration of DNA or RNA sequence by the rechnique of Sanger and colleagues, based on incorporation of the chain terminati
Formation of lactate and its consumption Formation of lactate and its consumption : If anaerobic conctitions prevail, the reoxidation of NADH through the respiratory cha
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