Embryonic stem cells (es), Biology

Assignment Help:

Embryonic stem cells (ES): These are pleuripotent cells of early embryo (blastocyst) capable of giving rise to all differentiated cells, including germ line cells. Embryonic stem cells (ES) can be cultured maintained and are able to proliferate. The method of producing transgenic mice by genetically modified embryonic stem cells is as follows:

(i)  Embryonic stem cell (ES) culture is initiated.

(ii)  The ES are transformed and screening is done in PCR analysis.

(iii)  Transformed cells can be cultured and inserted into blastocyst, which are then implanted in foster mother.

(iv)  Transgenic lines are determined by crosses from founder mice.


Related Discussions:- Embryonic stem cells (es)

What is crystallization of a virus, What is crystallization of a virus? Wha...

What is crystallization of a virus? What is the importance of this process? Crystallization is the method of transformation of viral components into organized solid particles.

Explain procedure for temporary mounts of fungal culture, Explain Procedure...

Explain Procedure for Temporary Mounts of Fungal Culture? (1) Observe mould culture for the colonial appearance and colour. Also observe the underside. (2) Label the clean,

What is waist-to-hip ratio, What is Waist-to-Hip Ratio? Waist-to-Hip Ra...

What is Waist-to-Hip Ratio? Waist-to-Hip Ratio (WHR) is defined as the measurement of waist circumference divided by hip circumference. For example, if a person's waist measure

What is chromosome mapping in phenotypic trait, What is Chromosome Mapping ...

What is Chromosome Mapping in phenotypic trait? Chromosome Mapping : By noting how often a gene segment associated with a specific phenotypic trait crossed over during meios

Define methods used in combination with pasteurization, Define methods used...

Define methods used in combination with pasteurization? Typical other methods used in combination with pasteurization include: 1.  Refrigeration as in the case of   milk

What is mechanical digestion, What is mechanical digestion? In molluscs, ar...

What is mechanical digestion? In molluscs, arthropods, earthworms, birds and vertebrates, in general, which organs respectively participate in this type of digestion? Mechanica

Nuclease, Nuclease  is an enzyme which degrades the nucleic acids. A nuclea...

Nuclease  is an enzyme which degrades the nucleic acids. A nuclease can be DNA-specific (a DNase), RNA-specific (RNase) or non-specific. It might act only on single stranded nuclei

Gumbout chiton, what is the classification of the Gumbout Chiton?

what is the classification of the Gumbout Chiton?

Explain the effect on minerals of the human milk, Explain the effect on Min...

Explain the effect on Minerals of the Human milk? There appears to be no relationship between dietary intake and concentrations in milk for copper, iron or zinc. Iron supplemen

Biota of littoral zone, Biota of Littoral Zone This zone is the shore ...

Biota of Littoral Zone This zone is the shore region of the marine ecosystems and is subject to violence of waves and tides, fluctuation of water level and variability of temp

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd