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Embryonic stem cells (ES): These are pleuripotent cells of early embryo (blastocyst) capable of giving rise to all differentiated cells, including germ line cells. Embryonic stem cells (ES) can be cultured maintained and are able to proliferate. The method of producing transgenic mice by genetically modified embryonic stem cells is as follows:
(i) Embryonic stem cell (ES) culture is initiated.
(ii) The ES are transformed and screening is done in PCR analysis.
(iii) Transformed cells can be cultured and inserted into blastocyst, which are then implanted in foster mother.
(iv) Transgenic lines are determined by crosses from founder mice.
Define Safety points that should used in the Laboratory? You should be aware of the rules/regulations and the procedures before undertaking any practical work in the laboratory
Diagnostic Procedures Taking Clinical history Urine analysis Blood sample for estimation of blood urea nitrogen Antistrepto lysine (ASO) titer
Similar to all animal cells, protozoans are covered by a plasma membrane which surrounds cytoplasm of the cell, protozoan's integument or skin. Like all membranes, it's permeable;
Q What do you mean by biology?
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