Electrophoresis is the technique of separating the large molecules (for instance DNA fragments or proteins) from the mixture of identical molecules. An electric current is passed through the medium containing mixture, and each type of the molecule travels through the medium at the dissimilar rate, depending on its electrical charge and size of it. The eparation is based on these differences. The agarose and acrylamide gels are the media generally used for electrophoresis of proteins and nucleic acids.