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Your methods so far have given some success! You now want to look at many different tissues in several individuals, some of whom have the mutation, some who are controls. How can you efficiently generate and amplify cDNA from each individual and tissue that can be used for directional cloning? Remember, you want to do many samples from many tissues, so starting with total cellular RNA fractions containing mRNA is where you need to put your effort and resources!
Conventional CABG on Cardiopulmonary Bypass Chest is opened by midline incision and median sternotomy. Simultaneously saphenous vein or radial artery is harvested. 'T
discuss the importance of protozoa to humans
Novel Sources of Natural Colourants Microbial sources Production of materials by microbial cultures has several advantages. The rapid growth of microbes cuts the productio
Define Assessment of iron status - Haemoglobin and Haematocrit? In the final stages of iron deficiency, anaemia occurs. Haemoglobin and Haematocrit levels indicate prevalence o
Q. What is Phytates? Phytates are widely distributed in seeds of vegetables, fruits and grains. Unrefined cereals and millets are the richest sources of phytates. Refined or
Treatment of Lyme Disease Lyme disease in North America is caused by the spirochete Borrelia burgdorferi, which is transmitted to humans by Ixodes scapularis or pacificus tick
Q. Do sponges have circulatory, nervous and excretory systems? Sponges do not have a nervous system neither excretory system nor circulatory system.
Which is the type of nitrogen waste birds produce? Why does this feature, besides being an adaptation to the terrestrial environment, also mean an adaptation to flight? Birds a
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Is there a difference between something that is alive and something that is a living organism?
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