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Your methods so far have given some success! You now want to look at many different tissues in several individuals, some of whom have the mutation, some who are controls. How can you efficiently generate and amplify cDNA from each individual and tissue that can be used for directional cloning? Remember, you want to do many samples from many tissues, so starting with total cellular RNA fractions containing mRNA is where you need to put your effort and resources!
Looking at what the academic world is publishing, microarray research is actually hot right now, and people haven't quite figured out what the best way is (some might argue if we s
What is the concept of Lophophore? A unique double ring of hollow ciliated tentacles that surround the oral opening in some animal phyla. There is some debate as to whether the
what are pathogenic characteristics of protoctista?
Explain the True indications of Endodontic Surgery 1. Failure of non-surgical endo. Re-treatment "persistent lesion" 2. Failure of initial and retreatment will not achieve b
What are the factors that limit our ability to fully classify and understand microbial diversity and what implications do these challenges have for environmental and health managem
What are the two divisions of the angiosperms? The angiosperms are separated into monocotyledonous and dicotyledonous. (These categories are defined later in this text.) Pla
Dideoxy Sequencing is the enzymatic determination and consideration of DNA or RNA sequence by the rechnique of Sanger and colleagues, based on incorporation of the chain terminati
Echocardiography is a very important tool in diagnosis of cardiovascular diseases and is used virtually in all categories of cardiovascular diseases. There are certain areas where
Gene targeting: There is always a debate on gene integration in to the host animal. It is random gene integration Vs gene targeting. Perhaps most significantly, pro-nuclear in
Define Clinical Manifestations and Nutritional Problems Associated with Cancer? In the previous section we learnt that cancer results in several changes in the metabolism of ca
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