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Your methods so far have given some success! You now want to look at many different tissues in several individuals, some of whom have the mutation, some who are controls. How can you efficiently generate and amplify cDNA from each individual and tissue that can be used for directional cloning? Remember, you want to do many samples from many tissues, so starting with total cellular RNA fractions containing mRNA is where you need to put your effort and resources!
Does the environment exert an influence on the phenotype? A phenotype may be changed (compared to the original situation conditioned by its genotype) by nongenetic means. Examp
what is ascus ?
The first step of the purification of an integral membrane protein is to disrupt its interactions with other integral proteins and the lipids in the membrane. This is commonly ach
A fifth member of the monoamine transmitter group is histamine. That release histamine as their neurotransmitter is located in the hypothalamus and project to cerebral cortex, lim
They result from septic embolization of vegetations to the arterial vasa vasorum or the intraluminal space, with subsequent spread of infection through the intima and outward throu
Didanosine (ddl, Videx) Didanosine is available as buffered tablets, buffered or non-buffered powder, and non-buffered enteric-coated capsules (Videx EC). Adverse effects
Valve Thrombosis : Valve thrombosis causes sudden deterioration of the patient's haemodyarnics. A stuck valve may produce both stenosis and incompetence. If diagnosed early s
TYPES OF CLEAVAGE - Depending upon the distribution & amount of yolk, cleavage is of following types - 1 . HOLOBLASTIC EQUAL CLEAVAGE ( holos , whole ; blastos, ger
H y po c a l c a e m i a It is also known as milk fever or parturient paresis. The disease occurs due to imbalance in input and output of calcium and is characterize
During the course of a physiology laboratory, a student finds that her PR interval is 0.24 second. Concerned, she takes her own ECG again an hour later and sees an area of the ECG
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