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Your methods so far have given some success! You now want to look at many different tissues in several individuals, some of whom have the mutation, some who are controls. How can you efficiently generate and amplify cDNA from each individual and tissue that can be used for directional cloning? Remember, you want to do many samples from many tissues, so starting with total cellular RNA fractions containing mRNA is where you need to put your effort and resources!
What is signified by saying that in relation to a given trait conditioned by a gene with two different alleles the gametes are always "pure"? To say so that the gametes are pur
What is Working of Mouth in human body? Food enters the digestive system through the mouth, where it is processed by the teeth and mixed with saliva, prior to being swallowed.
Sally Subcloner wants to clone a fragment into pUC19 (Map above – From New England Biolabs). Her fragment was a protein coding gene of 2.7 kb, and had XhoI sites on either end (and
What is karyotype? The name karyotype is given to the set of chromosomes of an individual, generally when visualized and identified under the microscope. The visualization usua
Illustrate about Whey protein Whey protein concentrate (WPC) is a highly nutritious ingredient manufactured from fresh dairy whey and it is spray dried to provide an excellent
What is light reaction? Light reactions are reactions, which are initiated by light (also photo-induced reactions). Usually, light energy is change into chemical energy.
A)Which of the following statements about DNA structure is true? 1.The nucleic acid strands in a DNA molecule are oriented ant parallel to each other, meaning they run in opposite
Define Briefly the Enzymatic Proteins? The most varied and very highly specialized proteins are those along with catalytic activity - the enzymes. Virtually all the chemical re
Q. List the stages of process of behaviour change? Stages of behaviour change process: · Unawareness · Awareness · Acquiring knowledge · Motivation · Trial
Q. Why do ribosomes move along mRNA during translation? During translation the ribosome always exposes two mRNA codons to be translated by moving along the mRNA, when a peptide
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