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Your methods so far have given some success! You now want to look at many different tissues in several individuals, some of whom have the mutation, some who are controls. How can you efficiently generate and amplify cDNA from each individual and tissue that can be used for directional cloning? Remember, you want to do many samples from many tissues, so starting with total cellular RNA fractions containing mRNA is where you need to put your effort and resources!
Write short notes on Urine? The concentrated filtrate produced by the kidney is called urine, and is composed mainly of water, urea, toxins, and mineral salts. Urine from the c
Role of Private Sector in Health Care One of the biggest policy challenges for the government is to decide on the appropriate areas and a suitable policy framework for having
Explain the Principles of Taxonomy? The goal of taxonomy is to develop a workable classification that reflects evolutionary relationships and provides identification and no
Define Consistency and Texture - Dysphilgia? Aspiration of liquids, including water, into the lungs can result in complications. To avoid this problem and yet fulfilling the fl
Cytokines activate inflammatory cells like neutrophils and eosnophils.IL-5 is important in activation of eosnophils, IL-4 enhances IgE production by B-cells. IL-4, Il-5, IL-6, TNF-
Explain the factors that lead to vitamin K deficiency? The factors that lead to vitamin K deficiency include: 1) Marginal dietary intake if one undergoes trauma and extensiv
Explain Properties of Natural fats and oils Natural fats and oils vary widely in their physical properties even though they are composed of the same or similar fatty acids. Th
Chemotaxis - Modes of Cell Movement Chemotaxis considers to me directed movement of cells in response to a concentration gradient of a chemical factor in solution. An instance
Cereal and Gram By-Products Agro-processing is now regarded as the sun rise sector in view of its large potential in maintaining the nutritional quality of processed feed resource
Glycerol can act as a substrate for glucose synthesis by conversion to dihydroxyacetone phosphate and an intermediate in gluconeogenesis. In order for citric acid cycle pyruva
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