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Your methods so far have given some success! You now want to look at many different tissues in several individuals, some of whom have the mutation, some who are controls. How can you efficiently generate and amplify cDNA from each individual and tissue that can be used for directional cloning? Remember, you want to do many samples from many tissues, so starting with total cellular RNA fractions containing mRNA is where you need to put your effort and resources!
What do facilitated diffusion and active transport have in common? What are the differences between them? Facilitated diffusion can be confused with active transport because in
GRAFFIAN FOLLICLE - It appears as knob or stigma in medulla. It is round, discovered by Graff. It is covered by 2 layers - thecae externa & theca interna. From theca i
why are life forms significant?
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Explain the Completed Test - Most Probable Number Test? Coliform colonies on EMB or Endo agar are further examined by completed test by inoculating lactose broth and nutrient a
Define the effect of Vitamins (A, D, K and B-Complex) on athletes? Vitamins A, D and K have been found to have no ergogenic effects. Ingesting large doses of these vitamins hav
Define about Calcium and Osteoporosis? Gain in bone mass occurs throughout childhood; however, during adolescent growth spurt, the gain in bone mass, as well as, calcium retent
Define Popularity of Chromatography - Basic Separation Technique? Chromatography is popular because: (a) minute quantities can be analysed qualitatively and quantitatively,
Define Micronutrient needs of a lactating mother? Vitamin and mineral deficiencies can have profound influence on the composition of milk. Calcium is a nutrient of special conc
Congenital impotentia genarandi Some bulls which are otherwise normal and healthy produce semen containing spermatozoa with specific morphological abnormalities together with
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