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Your methods so far have given some success! You now want to look at many different tissues in several individuals, some of whom have the mutation, some who are controls. How can you efficiently generate and amplify cDNA from each individual and tissue that can be used for directional cloning? Remember, you want to do many samples from many tissues, so starting with total cellular RNA fractions containing mRNA is where you need to put your effort and resources!
What is Calvin Cycle? The dark reactions of photosynthesis are also known as the Calvin cycle. This process synthesizes glucose molecules using carbon dioxide from the atmosphe
DNA Replication Deoxyribonucleic acid (DNA) is the carrier of genetic data for all living creatures. An organism's genome, made of DNA, encodes the genetic blueprint for buildi
Why is the Krebs cycle also called the final common pathway of the degradation of organic compounds? The Krebs cycle is known as the final common pathway of the degradation of
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Long-term responses - Behaviour of Plants Plants alter their course of development to suit the prevailing environmental conditions. For example, leaf maturation and fall, onse
Explain about the Net Protein Ratio (NPR)? This method was introduced by Bender and Doell (1957) and is a modification of the PER method. In this method, an allowance is made f
Q. How different are the swimming strategies in chondrichthyes and in osteichthyes? Why do sharks need to agitate their body to swim while bony fishes do not? Bony fishes have
Q. Why can mitochondria be considered the power plants of the aerobic cells? Mitochondria are the "power plants" of aerobic cells because within them the final stages of the ce
when the father and the mother of a newborn baby possesses blood group ''O'' +ve,is it possible that the baby can have a blood group ''A'' +ve or ''B''+ve ? Conventionally it shoul
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