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Your methods so far have given some success! You now want to look at many different tissues in several individuals, some of whom have the mutation, some who are controls. How can you efficiently generate and amplify cDNA from each individual and tissue that can be used for directional cloning? Remember, you want to do many samples from many tissues, so starting with total cellular RNA fractions containing mRNA is where you need to put your effort and resources!
what is osmoregulation.
Poultry Mycoplasma gallisepticum causes chronic respiratory disease (CRD) in chickens. The incubation period is 1-3 weeks. In young birds, the disease is less severe than adult
Incubator The incubators are essential to provide an ideal micro environment. The main functions are: isolation, maintenance of thermo neutral ambient temperature, desired h
Q. What is tertiary structure of a protein? What are the major types of tertiary structure? The tertiary protein structure is a spatial conformation additional to the secondary
Define Absorption, Storage and Elimination of cyanocobalamin? Vitamin B 12 in food is bound to proteins and is only released by the action of a high 'Concentration of hydroch
What is risk factor interaction ? Coronary artery disease, as has been explained, is a multifactorial disease with diverse risk factors coming together and interacting to produ
Explain xylose isomeras A mixture of glucose and fructose, is produced from sucrose by the action of yeast invertase (β-fructofuranosidase), an enzyme which can only be extrac
SPINAL NERVES 31 Pairs. Mixed type. Total weight 150 gms. At the base of origin of spinal nerves gland of swammardon or calcarious ganglion is present. Composed of m
What are the five conditons that can cause evolution to take place?
The symptoms and signs of endocarditis are often constitutional and, when localized, often result from a complication of IE rather than reflect the intracardiac infection itself. C
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