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Your methods so far have given some success! You now want to look at many different tissues in several individuals, some of whom have the mutation, some who are controls. How can you efficiently generate and amplify cDNA from each individual and tissue that can be used for directional cloning? Remember, you want to do many samples from many tissues, so starting with total cellular RNA fractions containing mRNA is where you need to put your effort and resources!
Explain the radiation cataract and steroid induced cataract of the eye. Radiation Cataract Exposure to radiation causes discoid posterior sub-capsular opacities. Mechanism o
Explain the Determination of Hydrolysis Hydrolysis of calcium pantothenate in acidic medium produces α,γ-dihydroxy-β,β-dimethylbutyrolactone, which reacts with hydroxylamine i
Degree of dispersion of protein solution is decreased. It is decreased because of certain reasons:- Association: refers to changes occuring at subunit or molecular leve
Ancillary Accomodation: Ward KitchenIPantry: It should have large sink and drain board along with fly proof door. If the food is received from central kitchen and tray pre
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what are floating plants? detailed information
Define about the Growth and development in children? Growth and development in children can be studied under the following heads: . • Changes in physical development and bod
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Determine the net charge of the predominant form Asp at (a) pH 1.0, (b) pH 3.0, (c) pH 6.0, and (d) pH 11.0?
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