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Your methods so far have given some success! You now want to look at many different tissues in several individuals, some of whom have the mutation, some who are controls. How can you efficiently generate and amplify cDNA from each individual and tissue that can be used for directional cloning? Remember, you want to do many samples from many tissues, so starting with total cellular RNA fractions containing mRNA is where you need to put your effort and resources!
It has been shown by prospective studies that Rheumatic heart disease (RHD) is linked to the occurrence of carditis during the first episode of ARF. If the first episode is accompa
Steps of Research Process: Identify the Problem: The first task in the planning stage is to identify and stage the problem, as you have already learnt in the earlier
Q. How does the sodium-potassium pump present in the cell membrane work? What is the significance of this protein for the cell? The sodium-potassium pump is the transport prote
How can I construct a fat molecule whose fatty acid tails are 4 carbon atoms long
a) What is 'vaso-constriction'? b) What are the effects of vaso-constriction in the skin? a) Vaso-constriction is the reduce in diameter of small arterioles an
Radiobiology : This is the study of effects of radiations on living organisms. Radiobiology is a branch of science which concerned with the action of ions. Radiobiology is the radi
You are given a hamster that has a very short tail (dominant trait) a) Describe a cross that would allow you to determine the short tailed hamster's genotype b) What are the possib
Describe the process of excitation-contraction coupling (ECC) in the heart. How do adrenaline, noradrenaline and acetylcholine alter heart rate and or force?
Corpus Cardiacum - Endocrine Organs We before mentioned that corpus cardiacum (pl. corpora cardiaca) is a neurohemal organ in insects. It is gland of neural origin. They are c
What is Phylum Hemichordata ? The name "hemi"; (meaning "half") provides a clue to these animals, which have some of the features of chordates, and some features that are chara
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