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Your methods so far have given some success! You now want to look at many different tissues in several individuals, some of whom have the mutation, some who are controls. How can you efficiently generate and amplify cDNA from each individual and tissue that can be used for directional cloning? Remember, you want to do many samples from many tissues, so starting with total cellular RNA fractions containing mRNA is where you need to put your effort and resources!
Explain Prophylaxis of lyme disease No vaccine is currently available to prevent Lyme disease in humans. Avoidance of ticks and use of tick repellents such as DEET or picaridin
Q. Pathophysiology of Chronic wasting disease? We all know that heart attack i.e. myocardial infarction is not the beginning but a last stage representing acute clinical manife
Hydration Properties General conformation of individual proteins in solution is largely dependent on the interaction with water. The progressive hydration of proteins startin
Can stroke be prevented Brain experts are convinced that several strokes can be prevented with proper attention to lifestyle factors which increase risk, including smoking, exc
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Anti Lipid Drugs : The current thinking is that it is very important to keep lipid levels low. It helps in preventing atherosclerotic changes in venous grafts and the long-term p
Define about the Paracellular Route - Calcium? It involves passive Ca transport through the tight junctions between mucosal cells. The salient features include: It is in
It is a hemolytic disease, which makes in newborn. Maternal IgG antibodies cross the placenta and destroy the red bleed cells. This develops when an Rh+ expresses an Rh antigen on
Q. Change in symptoms of aortic stenosis? Asymptomatic severe aortic stenosis patient needs to be followed once in a year or even more frequently. Patients with moderate aortic
Biota of Oceans - Ecosystem Life in the sea is not particularly abundant, though the diversity of organisms is very high. Almost every major group of animals and every major g
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