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Your methods so far have given some success! You now want to look at many different tissues in several individuals, some of whom have the mutation, some who are controls. How can you efficiently generate and amplify cDNA from each individual and tissue that can be used for directional cloning? Remember, you want to do many samples from many tissues, so starting with total cellular RNA fractions containing mRNA is where you need to put your effort and resources!
Q. What is represented by the cellular secretion? Cell secretion is the removal to the exterior of substances produced by the cell for instance, hormones, mucus, and sweat, so
ISOZYMES Sometimes an enzyme present in the same organism is found to have different molecular forms but catalyzing the same reaction. These are called isozymes. Th
HEAR T OUTPUT The amount of blood pumped by heart per minute. Heart beat is 72. Pump out blood is 70 ml. This 72 × 70 = 5040 ml. ( 5 litres) blood is pumped in a minute.
The normal flow pattern cross-mitral valve is a tall E wave where is due to early rapid filling and small A-wave which is due to atrial contraction. E/A ratio >1. Pulmonary
Endocrine System The nervous system brings about integration and co-ordination of several activities of the animal. The afferent stimuli from several sense organs are brought
Define Precautions for Determination of Haemoglobin Content in Blood 1. Potassium cyanide is highly toxic and hence drabkin's solution should not be pippeted by mouth. 2. Dr
Monosaccharides are frequently known as single or simple sugars and are the easiest of all carbohydrates. That is the building blocks of all higher carbohydrates. The Monosacchari
Explain Nephrotoxicity Nephrotoxicity is the major dose-limiting toxicity of amphotericin B deoxycholate; sodium loading with normal saline may prevent or ameliorate it and is
Teleology : It is the study of interpretations of structures in terms of utility and purpose. Teleology is philosophical account that holds the final causes exist in nature, meanin
Q. What is an instance of negative feedback of the homeostatic regulation? Negative feedback happens when the response to a given action generates an effect that inhibits that
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