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Your methods so far have given some success! You now want to look at many different tissues in several individuals, some of whom have the mutation, some who are controls. How can you efficiently generate and amplify cDNA from each individual and tissue that can be used for directional cloning? Remember, you want to do many samples from many tissues, so starting with total cellular RNA fractions containing mRNA is where you need to put your effort and resources!
What are the main biological functions of the polysaccharides? Polysaccharides have an energy storage function and a structural function. Polysaccharides incorporated by living
Nuclease is an enzyme which degrades the nucleic acids. A nuclease can be DNA-specific (a DNase), RNA-specific (RNase) or non-specific. It might act only on single stranded nuclei
How the age of a tree can be estimated from the analysis of the rings present on a cross section of its stem? For the growth of the tree it is essential to have formation of ne
In which period of life does the formation of gametes begin in women? The meiosis that forms female gametes begins in the cells of the ovarian follicles before birth. After the
Q. What are the tissues that form the skin in vertebrates? The skin of vertebrates is made of epidermis is an external layer of epithelial tissue and dermis a layer of connecti
Define Importance of Nutrient and Drug Interaction? Certain foods, beverages, alcohol, caffeine, and even cigarettes can interact with medicines. This may make them less effect
How to Prevent and Control PEM? Any programme aimed at prevention of PEM should be holistic and comprehensive considering the family as a unit. Since the effects of under nutr
Q. Fluid management in Diarrhoea? Fluid management: The key to diarrhoea management is the early replacement of fluid lost in the stools through intravenous or oral route. Whil
When William was helping victims following a devastating earthquake in a region that was not prepared to swiftly set up adequate temporary shelter, he developed severe diarrhea. He
Treatment of travelers'diarrhea The most common cause of travelers' diarrhea, usually a self-limited illness lasting several days, is infection with noninvasive enterotoxigeni
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