Efficiently generate and amplify cdna, Biology

Assignment Help:

Your methods so far have given some success! You now want to look at many different tissues in several individuals, some of whom have the mutation, some who are controls. How can you efficiently generate and amplify cDNA from each individual and tissue that can be used for directional cloning? Remember, you want to do many samples from many tissues, so starting with total cellular RNA fractions containing mRNA is where you need to put your effort and resources!

 

 


Related Discussions:- Efficiently generate and amplify cdna

What are the nitrate reductase and nitrite reductase tests, Based on your u...

Based on your understanding of the nitrate reductase and nitrite reductase tests, why would the inclusion of nitrite inhibit anaerobic growth?

What is the ploidy of these gamete-forming cells, What is the name of the c...

What is the name of the cells capable of making gametes? What is the ploidy of these gamete-forming cells? The cells that form gametes are the germ cells as opposed to the soma

Define excretion of toxin and act as precursor- carbohydrate, Define Excret...

Define Excretion of toxins and Act as precursors? Excretion of toxins: Glucuronic acid, a metabolite of glucose, combines with chemical and bacterial toxins and some normal met

Spinal nerves, Spinal nerves: They take their origin from the spinal...

Spinal nerves: They take their origin from the spinal cord. They are the mixed nerves having both sensory and motor fibres. There are 31 pairs of spinal nerves. The se

Secondary and tertiary consumers - biotic components, Secondary and Tertiar...

Secondary and Tertiary Consumers - Biotic Components Besides there are animals which depend on herbivores for food and are called secondary consumers. Since secondary consumer

TAKHTAJANS classification, comparison between takhtajans and natural classi...

comparison between takhtajans and natural classification

Find the magnitude and direction of the net electric force, Three point cha...

Three point charges are located at the corners of an equilateral triangle as in the figure below. Find the magnitude and direction of the net electric force on the 1.40 µC charge.

Syncytial theory - metazoa, Syncytial Theory - Metazoa This theory su...

Syncytial Theory - Metazoa This theory suggests that the ancestral metazoan was at ,first Syncytial in structure but later became cellularised by formation of cell membranes

Subphylum opalinata, Subphylum Opalinata Body covered with longitudin...

Subphylum Opalinata Body covered with longitudinal rows of cilium like ,organelles but true ciliature absent. Parasitic; cytosome lacking; two to many nuclei of one type.

Explain the neuritic type of infantile beriberi, Explain the neuritic type ...

Explain the neuritic type of infantile beriberi? It is also referred to as Wernicke korsakoff syndrome or cerebral beriberi. It shows typical manifestations of peripheral neuro

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd