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Your methods so far have given some success! You now want to look at many different tissues in several individuals, some of whom have the mutation, some who are controls. How can you efficiently generate and amplify cDNA from each individual and tissue that can be used for directional cloning? Remember, you want to do many samples from many tissues, so starting with total cellular RNA fractions containing mRNA is where you need to put your effort and resources!
Define Procedure for Testing the Presence of Proteins in Milk? 1. Add 0.5 ml of alcohol to 0.5 ml milk. Alcohol helps in curdling the milk. 2. Observe for the curdling of m
Explain Sickle cell anemia If 4% of a people are born with a severe form of sickle cell anemia. what percent of population will be resistant to malaria in spite of carrying
Reflexes It is used to assess the neurobehavioural status. Rooting reflex - Being a primitive reflex, if cheek is rubbed, the infant will turn his head into that dire
A laboratory mouse homozygous for an RFLP marker is mated to a wild mouse that is heterozygous for that marker. One of the heterozygous individuals resulting from this cross is mat
What are steroids? What are some examples of steroids with a biological function? Steroids are lipids based in an angular combination of four carbon rings, three of them made o
Define the Recommended Dietary Allowance for Vitamin B 12 (RDA)? Vitamin B 12 deficiency is common in true vegans who can be treated with small doses since the daily requirem
Transport of Gases in Blood We know that two types of gas exchanges are constantly occurring in the animal body--one at the inter phase of the respiratory membrane and externa
Vesicular stomatitis Vesicular sto matitis virus be longs to the ge nus Vesiculovirus in fa mily Rhabdoviridae. The disease clinically resembles foot-and-mouth disease, but is
If two organisms share the similar kingdom, must they also share the similar domain? Explain. Two organisms that share the similar kingdom must also share the similar domain b
VACUOLES Some animals cells may have one to a few small fluid filled vacuoles.. Plants cells in general possess one or a few large vacuoles. Vacuoles of animals cells
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