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Your methods so far have given some success! You now want to look at many different tissues in several individuals, some of whom have the mutation, some who are controls. How can you efficiently generate and amplify cDNA from each individual and tissue that can be used for directional cloning? Remember, you want to do many samples from many tissues, so starting with total cellular RNA fractions containing mRNA is where you need to put your effort and resources!
Is fecundation in amphibians external or internal? In this aspect are amphibians evolutionarily proximal to fishes or to reptiles? In the majority of the amphibian species fec
What are the basic constituents of the cell membrane? The cell membrane is produced of lipids, proteins and carbohydrates. The membrane lipids are phospholipids, a special t
Q. What are plasmids? And what is the significance of plasmids for the recombinant DNA technology? Plasmids are circular fragments of DNA that are accessories to the main bacte
Define the Disc Electrophoresis? Disc electrophoresis has improved resolution and strong bands are obtained. It requires a two-gel system and several different buffers. The run
Q. Show the Signs and symptoms of implant failure? Signs and symptoms of implant failure are : 1. Horizontal mobility beyond 0.5 mm or any clinically observed vertical move
what is ribosomes
What do all organic compounds contain? The key element is carbon. Organic compounds are all carbon-having compounds. By definition, an organic compound having carbon. Alt
What is signified by saying that in relation to a given trait conditioned by a gene with two different alleles the gametes are always "pure"? To say so that the gametes are pur
Determination of vitamin c Majority of chemical methods of determination is based on the rapid oxidation of ascorbic acid and therefore not too highly specific. Titration of
Diet : Pre-operatively, patients are on low calorie, low fat diet. However in the immediate post-operative period, strict dieting is not advisable. They need good nutrition for p
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