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Your methods so far have given some success! You now want to look at many different tissues in several individuals, some of whom have the mutation, some who are controls. How can you efficiently generate and amplify cDNA from each individual and tissue that can be used for directional cloning? Remember, you want to do many samples from many tissues, so starting with total cellular RNA fractions containing mRNA is where you need to put your effort and resources!
Why Chordates are Schizocoelic, while other deuterostomes are enterocoelic?
Suppose many of the atoms of nitrogen in a double-stranded molecule of DNA, of isotope N-14, were replaced with the isotope N-15. What afffect would this have? a) The nitrogeneo
The synthesis of the three fundamental polymers of life, DNA, RNA and protein, is template-directed. This means that - Answer A. the monomers are assembled one by one in a sequence
Q. Illustrate goal of modern dentistry? The goal of modern dentistry is to restore the patient to normal contour, comfort, function, esthetics, speech and health regardless of
Define Components of Total parenteral nutrition? Glucose: Initiated at the rate of 6 mg/kg/min and increased upto 12-1.4 mg/kg/min, but care to be taken to prevent hyperglyce
Choice of graft material depends on - Its nature towards bone e.g., Osteoconductive or osteoinductive - Its architecture i.e., granular or block, pore si-e - Its origin -
why frog respire through skin
Noncompetitive inhibition In this type of inhibition, the inhibitor binds at a site on the enzyme other than catalytic site. As there is no competition between the s
What is Kingdom Monera? The eubacteria, archaebacteria, and blue-green bacteria (or cyanobacteria) which form Kingdom Monera lack the cellular organization that all other livin
Which of the following statements regarding the interactions among sequence-specific DNA binding domains and their recognition sites is incorrect? A. Sequence-specific DNA bind
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