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Your methods so far have given some success! You now want to look at many different tissues in several individuals, some of whom have the mutation, some who are controls. How can you efficiently generate and amplify cDNA from each individual and tissue that can be used for directional cloning? Remember, you want to do many samples from many tissues, so starting with total cellular RNA fractions containing mRNA is where you need to put your effort and resources!
The reported occurrence of ICMAs is 1.2 per cent to 5 per cent of cases. Streptococci and S.aureus account for 50 per cent and 10 per cent of cases, respectively, and are seen with
Brooke's Formula: a) Fluid requirement b) Estimate the accurate/approximate weight of the patient c) First 24 hours Colloids (blood, plasma, dextran) 0.5 ml
Patient X has blood plasma levels of Parathyroid Hormone (PTH) that are always very high due to a tumor consisting of Parathyroid Gland cells that secrete high levels of PTH into t
Endodontic success - According to Strindberg 1956 the endodontic success is the postoperative clinical as well as radiographic satisfaction after a predetermined postoperative
Q. What do you mean by Periodical? A periodical is a publication appearing at regular intervals. Each issue is called a number and collectively these numbers comprise a volume.
Give the introduction to evaluation of patient clinical examination ? A careful physical examinations is a very important for assessing the cardiovascular system and after prov
What do you understand by the terms retrogradation and gelatinization? The realignment of the amylose and amylopectin and swollen starch granules to form a pocket is termed
The genotype of individual is EeFfGgHh, while hte genotype of individual #2 is EeFfggHH. A cross is performed between individual 1 and 2. What is the propability of producing the f
Define the Cooper's Run (12 minute run) Method? To undertake this test, a 400 metre running track is required-marked every 100 m and a stop watch. The test comprises of noticin
How is genetic engineering used to create bacteria capable of producing human insulin? In a production of human insulin by bacteria the human insulin gene is incorporated into
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