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Your methods so far have given some success! You now want to look at many different tissues in several individuals, some of whom have the mutation, some who are controls. How can you efficiently generate and amplify cDNA from each individual and tissue that can be used for directional cloning? Remember, you want to do many samples from many tissues, so starting with total cellular RNA fractions containing mRNA is where you need to put your effort and resources!
Define Equilibrium between two solid phases and a gas phase? Consider the following reaction equilibrium: 3 CuO(s) + 2NH 3 (g) ↔ 3 Cu(s) + 3H 2 O (g) + N 2 (g) Accordin
What is vena cava? Which type of blood circulates within the vena cava? The vena cava are either of two large veins that debouch into the right atrium. The superior vena cava d
In yeast, a new mutation called suc was recently discovered. The suc mutant cannot use sucrose as a source of carbon, and will not grow if the culture medium contains only sucrose
Two parents who are each known to be carriers of an autosomal recessive alleles have four children. None of the children has the recessive condition. What is the probability that o
Explain why Meat products causes diabetics Diabetics can have meat products in case they eat non-vegetarian food. Baking, roasting or grilling is preferable to frying. Patient
The sequence for the Beta Actin gene (ACTB) is given below. Which pair of primers (designed to amplify the entire sequence below) could be used to successfully produce the beta act
Describe two differences among green algae and plants. Algae lack tissue differentiation and have no true roots, leaves and stems. The gametangia of algae are single-celled; th
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Stage 2 implant surgery After evaluating the status of the implant, stage two procedure is carried out to bring forth a communication of the implant to the oral cavity by surgi
Comparing toxicity and the requirement for dilution in water how different are the ureotelic and the uricotelic excretions? What are some examples of animals that present these res
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