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Your methods so far have given some success! You now want to look at many different tissues in several individuals, some of whom have the mutation, some who are controls. How can you efficiently generate and amplify cDNA from each individual and tissue that can be used for directional cloning? Remember, you want to do many samples from many tissues, so starting with total cellular RNA fractions containing mRNA is where you need to put your effort and resources!
Hypomagnesaemic tetany of calves Young calves in the age group of 2-8 weeks and fed whole milk or milk replacer are more likely to develop hypomagnesaemia due to inadequate ma
Axon: Axon is a projection from the cell body. Each neuron has only one axon. Unlike dendrites, axons are very long and are usually unbranched structures. The axon
Q. How are inhalation and expiration carried out? The diaphragm exclusive of mammals and the intercostals muscles can contract or relax varying the volume of the thorax the com
Factors Limiting the Productivity of Aquatic Habitats Sunlight and oxygen are the two most important limiting factors of the aquatic ecosystems. This distinguishes them from t
For a sarcomere of a skeletal muscle, define the following terms: A is the length of the A Band; H is the length of the H Zone; I is the total length of the I Bands in the sarcomer
Asymptomatic patient: The current opinion is that in asymptomatic patients with left ventricular dysfunction and severe MR surgery should not be delayed. The echo cardiographic a
Ethnobotany : This is the study of relationship between tribals and plants. Ethnobotany is from "ethnology" - study of culture or "botany" - study of plants. This is the scientific
Implementation of Nursing Care Isolate the Child: When the child is admitted with suspected meningitis, the nurse has to take care that child's isolation is essential to p
Phosphorylation of glucose Glucose is converted to glucose-6-phosphate since phosphorylated intermediates do not readily penetrate cell membrane and this commits glucose t
Bone Remodeling Bone remodeling differs from the other means of bone structure alteration in that osteoblasts and Osteoclasts do not act independently but are coupled and bone
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