Dot blot, Biology

Assignment Help:

Dot blot is the technique for measuring the amount of one perticular DNA or RNA in the highly complex mixture. The samples are spotted onto the hybridization membrane (like nitrocellulose or activated nylon, and many more), fixed and hybridized with the radioactive search. The extent of labeling (as determined by autoradiography and the densitometry) is proportional to concentration of the target molecule in its sample. Standards give the means of calibrating the results. 


Related Discussions:- Dot blot

Define briefly about the pyridoxine vitamin, Define Briefly about the Pyrid...

Define Briefly about the Pyridoxine vitamin B 6 ? Pyridoxine or vitamin B 6 is one of the B complex vitamins which prevents and cures dermatitis in rats fed on vitamin B 6 de

Taxonomy, Agroup of realated genera are classified as-?

Agroup of realated genera are classified as-?

Define denaturation, Denaturation: With respect to the nucleic acids, refe...

Denaturation: With respect to the nucleic acids, refers to the conversion from the double-stranded to single-stranded state, many times achieved by heating or alkaline conditions.

What is the meaning of term - plasticity, What is the meaning of term - Pla...

What is the meaning of term - Plasticity The notion of brain plasticity has been of interest to researchers and clinicians alike for decades. The outcome of injury is the resul

Animal around us, #question.what are the reason that arthropodans are abund...

#question.what are the reason that arthropodans are abundant in nature.

Define the effect of dietary fibre on nutrient absorption, Define the effec...

Define the effect of dietary fibre on Nutrient Absorption? Nutrient Absorption: Inclusion of fibre has been shown to retard the absorption of some nutrients. The inclusion of v

What''s the difference between heterozygosity & homozygosity, What is the d...

What is the difference between heterozygosity and homozygosity? The Homozygosity occurs when an individual has two identical alleles of a gene, for instance, AA or aa. The Het

Explain echinocandins, Explain Echinocandins It inhibit synthesis of β ...

Explain Echinocandins It inhibit synthesis of β (1,3)-D-glucan, an essential component of the fungal cell wall. The potential for adverse effects in humans is low because of th

Define protection of poly unsaturated fatty acids, Define Protection of pol...

Define Protection of poly unsaturated fatty acids (PUFA) from oxidative damage? The major biological role of vitamin E is to protect PUFAs and other components of cell membrane

Illustrate dilated cardiomyopathy, Q. Illustrate Dilated cardiomyopathy? ...

Q. Illustrate Dilated cardiomyopathy? It is a disease of unknown etiology, affecting myocardium. Its diagnosis is established by presence of left ventricular dilatation and sys

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd