Dot blot, Biology

Assignment Help:

Dot blot is the technique for measuring the amount of one perticular DNA or RNA in the highly complex mixture. The samples are spotted onto the hybridization membrane (like nitrocellulose or activated nylon, and many more), fixed and hybridized with the radioactive search. The extent of labeling (as determined by autoradiography and the densitometry) is proportional to concentration of the target molecule in its sample. Standards give the means of calibrating the results. 


Related Discussions:- Dot blot

Define commodity topics of food science, Define Commodity Topics of Food Sc...

Define Commodity Topics of Food Science? All issues of food science and technology of certain commodity groups, including milk and milk products (fluid milk and derivatives, ic

Function of cholecystokin in the digestive process, Q.How is it produced an...

Q.How is it produced and what is the function of cholecystokin in the digestive process? The fat level of the chyme detected in the duodenum stimulates the secretion of cholecy

Freeze fracture electron microscopy, Freeze Fracture Electron Microscopy ...

Freeze Fracture Electron Microscopy Freeze fracture electron microscopy i a technique lo reveal the surface features of hydrophobic interior of cell membrane. In this technique,

Chemical methods of estimating available nutrients, Chemical Methods of Est...

Chemical Methods of Estimating Available Nutrients  Water soluble forms of nutrients are available to plants but forms other than water soluble may also become available to pla

What are the nine phyla of the kingdom animalia, Q Which are the beings tha...

Q Which are the beings that form the kingdom Animalia? What are the two big groups into which this kingdom is divided? The kingdom Animalia is the animal kingdom. usually the k

Health benefits of dietary factors - antinutritional effect, Define Health ...

Define Health Benefits of Other Dietary Factors with Antinutritional Effects? Despite the predominantly nutritional antagonistic effects of the factors described above, there i

What is density-dependent and density-independent factors, What is Density-...

What is Density-dependent and density-independent Factors? Density-dependent : Ecologists identify population-regulating mechanisms whose functioning is related to density a

Define dietary management of cancer patients, Define Dietary Management of ...

Define Dietary Management of Cancer Patients and Feeding Problems Related To Cancer Therapy? We took an overview about the general nutritional requirements of cancer patients.

Define the operations of a public nutritionist, Define the Operations of a ...

Define the Operations of a Public Nutritionist? A public nutritionist can perform the following: In the hospital-based set up, she is a part of the team delivering thera

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd