Dot blot, Biology

Assignment Help:

Dot blot is the technique for measuring the amount of one perticular DNA or RNA in the highly complex mixture. The samples are spotted onto the hybridization membrane (like nitrocellulose or activated nylon, and many more), fixed and hybridized with the radioactive search. The extent of labeling (as determined by autoradiography and the densitometry) is proportional to concentration of the target molecule in its sample. Standards give the means of calibrating the results. 


Related Discussions:- Dot blot

Smoking of meat products, S m o k in g of Meat Products The purpos...

S m o k in g of Meat Products The purpose of smoking meat is to develop a distinctive flavour, aroma and appearance. These attributes can also be achieved with liquid smok

Explain senning procedure, Explain Senning Procedure ? Operation is per...

Explain Senning Procedure ? Operation is performed by ascending arteries and separate SVC and IVC cannulation. If the operation is done under deep hypothermic circulatory arres

A light microscope with unlimited resolution, Theoretically you can build a...

Theoretically you can build a light microscope with unlimited magnification, but can you build a light microscope with unlimited resolution? Why or why not? (Hint: what really cont

What is oxidative phosphorylation, What is oxidative phosphorylation? O...

What is oxidative phosphorylation? Oxidative phosphorylation is  the process by which ADP is phosphorylated by Pi  to ATP  in  the  respiratory chain.

Action of hormones, Action of Hormones We said earlier that hormones a...

Action of Hormones We said earlier that hormones are released into the blood stream or extracellular fluid and therefore, reach most of the cells of the body. However, they

Explain micafungin, Explain Micafungin It is an echinocandin indicated ...

Explain Micafungin It is an echinocandin indicated for treatment of esophageal candidiasis and prophylaxis of invasive Candida infections in patients undergoing hematopoietic s

Define protein requirement for cancer patients, Define Protein Requirement ...

Define Protein Requirement for Cancer Patients? Both the metabolic stress of cancer, as well as, chemotherapy results in increased tissue catabolism. Hypoalbuminemia and anaemi

Plant physiology, what is the importance of lignin in xylem formation

what is the importance of lignin in xylem formation

Write in brief about hba1c, Write in brief about HbA1c. HbA1c is also t...

Write in brief about HbA1c. HbA1c is also the primary glycemic (sugar) target in the management of a diabetic patient and should be performed at repeated intervals throughout t

How is cancer usually treated, Q. How is cancer usually treated? If the...

Q. How is cancer usually treated? If the cancer is in its initial stage cure is often done by surgical removal of the neoplastic tissue. The Cancers already disseminated are of

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd