Distribution coefficient -terminology used in chromatography, Biology

Assignment Help:

Distribution Coefficient - Terminologies used in Chromatography?

During the purification or separation of the biomolecules it should be kept in mind that two important factors influence the resolution (separation) of a mixture of compounds. These are effective distribution coefficient (i.e. the ratio of the amount present in one phase divided by the amount present in the other phase) and the length of the column. Thus, the basis for all forms of chromatography is the partition or distribution coefficient, wherein a compound distributes itself between two immiscible phases. For a compound distributing itself between equal volumes of two immiscible solvents A and B, the value of this coefficient is constant at a given temperature. The distribution coefficient can thus be described as:

Distribution coefficient/partition coefficient = Concentration in solvent A/ Concentration in solvent B


Related Discussions:- Distribution coefficient -terminology used in chromatography

Natural selection, Darwin put fort the notion of the survival of the fittes...

Darwin put fort the notion of the survival of the fittest. How do we define 'fittest'? The answer usually given is those who survive. So you can readily see that this is a circular

Into which classes are mollusc divided, Q Into which classes are mollusc di...

Q Into which classes are mollusc divided? What are some representing beings of each class? The phylum Mollusca is divided into five main classes: pelecypods, or bivalves (Pelec

Define about the pseudosquint, Define about the Pseudosquint (Pseudo-false...

Define about the Pseudosquint (Pseudo-false) Pseudosquint is the condition when despite the visual axis being parallel, there , appears to be a squint. Pseudosquint can be conv

Sterigmatocystin, These mycotoxins are structurally and biologically relat...

These mycotoxins are structurally and biologically related to the aflatoxins, and like the latter, they cause hepatocarcinogenic activity in animals. Among the organisms that prod

Define arterial vessels - arterioles and arteries, Q. What are arterial ves...

Q. What are arterial vessels, arterioles and arteries? Arterial vessels are every blood vessel that carries blood from the heart to the tissues, arterioles and Arteries are art

Symptoms and complications of atherosclerosis, Q. Symptoms and Complication...

Q. Symptoms and Complications of atherosclerosis? Symptoms Excessive weight, hypertension, high levels of cholesterol and triglycerides. Complications Myocardia

Paper points & chemicals removal of gutta percha, Paper points & chemicals ...

Paper points & chemicals removal of gutta percha: -  Flushing action: residual of qutta- percha and sealers chemically solvent by floating pulp chamber with chloroform. -  Chem

Restriction endonuclease, Suppose the restriction endonuclease HindIII cuts...

Suppose the restriction endonuclease HindIII cuts a6.0 kb linear piece of DNA into two fragments; an 800 bp fragment and a 5200 bp fragment..... Question: Suppose restriction

Define the neurological disorders of dysphagia, Define the neurological dis...

Define the neurological disorders of dysphagia? In neurological disorders, damage to cranial nerves can lead to weak and poorly co- ordinated tongue movements, which results in

What is reduction, What is reduction? The most commonly understood defi...

What is reduction? The most commonly understood definition of reduction is the removal of oxygen or addition of hydrogen ions. However, chemically, reduction can be defined as

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd