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Dideoxy Sequencing is the enzymatic determination and consideration of DNA or RNA sequence by the rechnique of Sanger and colleagues, based on incorporation of the chain terminating dideoxynucleotides in a growing nucleic acid strand copied by DNA polymerase or the reverse transcriptase from a DNA or RNA template. Different reactions involve dideoxynucleotides containing A, C, G, or the T bases. The reaction products represent the collection of new labeled DNA strands of the varying lengths, all terminating with the dideoxynucleotide at the 3' end (at site of a complementary base in the template nucleic acid), and are separated in the polyacrylamide or urea gel to generate the sequence "ladder". This technique is more commonly used than the "Maxam-Gilbert" (chemical) sequencing.
Viral vectors There are a large number of viral vectors available which can transfer the desired sequence properly into the host. Examples of the viral vectors are as follows
Determine by nutritive muscular cell? The cells which form the gastrodermis lining the inner cavity of cnidarians. They carry out two functions: First is to absorb and digest f
Haploid Production The higher plants are normally diploid, with two sets of chromosomes in their somatic cells. Their haploids (with one set of chromosomes) arise in nature by
What is ventilation A. An increase in the hydrogen ion concentration in the interstitial spaces of the brain stem leads to an increase in the duration of the respiratory cy
Q. Why is it dangerous to talk while eating? If a person talks when eating, food may be inhaled into the lungs. Generally after food is swallowed, it passes into pharynx and th
What is the heterotrophic hypothesis on the origin of life? As per to the heterotrophic hypothesis the first living beings were very simple heterotrophic organisms that is not
ENZYMES OF E.R. Numerous enzymes have been found associated with its membranes, Glucose-6-phosphatase is the marker enzyme of E.R. The chief being those required for the synt
The conventional approach to design earthquake resistant building is based upon equipping the building with stiffness, strength and deformation due to elasticity which can afford a
what happens to your total peripheral resistance when you go from a warm room to cool outside air
Define Advantages of Direct Microscopic Count? 1. It is easy, inexpensive and relatively quick method. 2. It gives information regarding size and morphology of microorganism
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