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Dideoxy Sequencing is the enzymatic determination and consideration of DNA or RNA sequence by the rechnique of Sanger and colleagues, based on incorporation of the chain terminating dideoxynucleotides in a growing nucleic acid strand copied by DNA polymerase or the reverse transcriptase from a DNA or RNA template. Different reactions involve dideoxynucleotides containing A, C, G, or the T bases. The reaction products represent the collection of new labeled DNA strands of the varying lengths, all terminating with the dideoxynucleotide at the 3' end (at site of a complementary base in the template nucleic acid), and are separated in the polyacrylamide or urea gel to generate the sequence "ladder". This technique is more commonly used than the "Maxam-Gilbert" (chemical) sequencing.
What is a cytoskeleton? What are its main constituents in animal cells? Cytoskeleton is the cytoplasmic structure that handles the cell, keeps its shape and fixates and moves t
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What are the three basic sexual life cycles studied in Biology? Which of them corresponds to metagenesis? Which of them is the human life cycle? Sexual reproduction may take pl
Determine the significance of Siphuncle. A cord of tissue that passes between different chambers of a Nautilid shell. Siphuncle regulates the amount of air or fluid in chambers
H y p o ma g n e s e m i a It is also known as grass staggers or lactation or grass tetany and results in clonic and tonic muscular spasms and convulsions. E
S h o c k It is defined as a generalized acute reduction in the perfusion of tissues by which there is oxygen deficiency in the cells. It is characterized by reduction in e
What is the antagonism between the sympathetic and the parasympathetic neural actions? In general the actions of the sympathetic and the parasympathetic are antagonistic, i.e.,
Consider a time when the membrane voltage of a SA node cell in the heart is at its minimum value (near -80 mv). At this time, A. all the voltage-gated calcium channels will be
Q. What are the "chiasms" of homologous chromosomes observe in prophase I? Chiasms are intersections of two tracts in the form of X, The chiasms seen in prophase I are chromoso
Xerophytes Xerophytes are sometimes loosely defineri as 'plants of dry habitats'. But others have defined xerophytes as 'plants which grow on the substratum that usually beco
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