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Dideoxy Sequencing is the enzymatic determination and consideration of DNA or RNA sequence by the rechnique of Sanger and colleagues, based on incorporation of the chain terminating dideoxynucleotides in a growing nucleic acid strand copied by DNA polymerase or the reverse transcriptase from a DNA or RNA template. Different reactions involve dideoxynucleotides containing A, C, G, or the T bases. The reaction products represent the collection of new labeled DNA strands of the varying lengths, all terminating with the dideoxynucleotide at the 3' end (at site of a complementary base in the template nucleic acid), and are separated in the polyacrylamide or urea gel to generate the sequence "ladder". This technique is more commonly used than the "Maxam-Gilbert" (chemical) sequencing.
What is Single S 2 In cyanotic TOF, S, consists entirely of A, as P, is attenuated by a deformed pulmonary valve when there is valvar steuosis and aorta is placed anteriorly re
Primary Egg Envelopes These are the egg envelopes which develop in the ovary between oocyte and follicle cells in the space occupied by the interdigitating microvilli. Such e
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What is the other name given to sex chromosomes? Sex chromosomes are also known as allosomes (the other chromosomes that are not sex chromosomes are known as autosomes).
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