Dideoxy sequencing, Biology

Assignment Help:

Dideoxy Sequencing is the enzymatic determination and consideration of DNA or RNA sequence by the rechnique of Sanger and colleagues, based on incorporation of the chain terminating dideoxynucleotides in a growing nucleic acid strand copied by DNA polymerase or the reverse transcriptase from a DNA or RNA template. Different reactions involve dideoxynucleotides containing A, C, G, or the T bases. The reaction products represent the collection of new labeled DNA strands of the varying lengths, all terminating with the dideoxynucleotide at the 3' end (at site of a complementary base in the template nucleic acid), and are separated in the polyacrylamide or urea gel to generate the sequence "ladder". This technique is more commonly used than the "Maxam-Gilbert" (chemical) sequencing.


Related Discussions:- Dideoxy sequencing

Briefly discuss the salmonella food infection, Briefly discuss the Salmonel...

Briefly discuss the Salmonella food infection. • Salmonella food infection is caused by Salmonella gastroenteritis. Transmitted by faecal contamination of foods. In

Explain thermal decomposition heating of food, Explain Thermal Decompositio...

Explain Thermal Decomposition Heating of food Thermal Decomposition Heating of food produces various chemical changes, some of which can be important to flavour, appearance, nu

Heat-shock response, Heat-shock Response When growing plantlets or tis...

Heat-shock Response When growing plantlets or tissues of plants are shifted to 42°C and above, the synthesis of normal proteins rapidly declines and instead a set of new prote

Total artificial heart in circulatory assist devices, Total Artificial Hear...

Total Artificial Heart :  Jarvik seven was the first successful total artificial heart supporting the patient for 112 days. The disadvantage is that the patient has to be connecte

Indications for surgery, Indications for Surgery :  Patients with moderate...

Indications for Surgery :  Patients with moderate or severe tricuspid regurgitation that is functional will need tricuspid annuloplasty at the time of correction of mitral valve d

The deficiency in hepatic glucose 6 phosphatase, Under fasting conditions, ...

Under fasting conditions, individuals with a deficiency in hepatic glucose 6 phosphatase cannot dephosphorylate glucose 6 phosphate generated from liver glycogenolysis. As a result

Explain magnitude and direction of the electrostatic force, Three charges a...

Three charges are arranged as shown in the figure below. Find the magnitude and direction of the electrostatic force on the charge q = 4.78 nC at the origin. (Let r12 = 0.260 m.)

Causes of air pollution, Causes of Air Pollution We known about the c...

Causes of Air Pollution We known about the composition of atmosphere, its major and minor constituents. The composition of atmosphere has remained the same for thousands of y

Nutritiona, Describe the tasks of various body systems in breaking down car...

Describe the tasks of various body systems in breaking down carbohydrates into glucose to fuel the cells work

What do you mean by trachea, Q. Why doesn't the food enter the trachea inst...

Q. Why doesn't the food enter the trachea instead of going to the esophagus? When food is swallowed the swallow reflex is activated and the larynx closes and elevates to avoid

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd