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Dideoxy Sequencing is the enzymatic determination and consideration of DNA or RNA sequence by the rechnique of Sanger and colleagues, based on incorporation of the chain terminating dideoxynucleotides in a growing nucleic acid strand copied by DNA polymerase or the reverse transcriptase from a DNA or RNA template. Different reactions involve dideoxynucleotides containing A, C, G, or the T bases. The reaction products represent the collection of new labeled DNA strands of the varying lengths, all terminating with the dideoxynucleotide at the 3' end (at site of a complementary base in the template nucleic acid), and are separated in the polyacrylamide or urea gel to generate the sequence "ladder". This technique is more commonly used than the "Maxam-Gilbert" (chemical) sequencing.
What is the importance of water for enzymatic activity? Enzymes, biological catalysts, rely on water to reach their substrates and bind to them. There is no enzymatic activity
BON E MARROW TRANSPLANT - It is transplanted to treat leukemia. Here, graft itself causes immune rejection. Before transplant, the recipient's bone marrow cells, incl
Explain Polio Adults who have not previously been immunized against polio should receive a primary series of inactivated polio vaccine (IPV) if traveling to areas where polio i
Which one of the following statements about certain given animals is correct? 1. Round worms (Aschelminthes) are pseudocoelomates 2. Molluscs are acoelomates 3. Insects ar
PEROXISOMES AND GLYOXYSOMES Certain small ovoid unit membrane bound sac like cytoplasmic organelles, resembling lysosomes in size and appearance were isolated from var
Explain Lipid Transport in Nutritional Care? Proteins provide the transport mechanism for lipids by forming lipoproteins. This helps to prevent fatty infiltration and hence pro
Strawberries are quick frozen at a rate of 6500 kg/h. The fruit enters the freezer at a temperature of 15°C and is frozen to a final temperature of -20°C. Calculate the rate of hea
herterofertilization
How does the heart impel the blood? The heart is a muscular organ that having chambers (right atrium and right ventricle and left atrium and right ventricle) by which blood pas
Q. Explain the types of classification of plants? It is practically impossible for anyone to study all the plants of the world, 'even if one spends whole life. Thus, it is nece
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