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Dideoxy Sequencing is the enzymatic determination and consideration of DNA or RNA sequence by the rechnique of Sanger and colleagues, based on incorporation of the chain terminating dideoxynucleotides in a growing nucleic acid strand copied by DNA polymerase or the reverse transcriptase from a DNA or RNA template. Different reactions involve dideoxynucleotides containing A, C, G, or the T bases. The reaction products represent the collection of new labeled DNA strands of the varying lengths, all terminating with the dideoxynucleotide at the 3' end (at site of a complementary base in the template nucleic acid), and are separated in the polyacrylamide or urea gel to generate the sequence "ladder". This technique is more commonly used than the "Maxam-Gilbert" (chemical) sequencing.
Heterophagic intracellular digestion is the breaking into minute substances of external substances engulfed in the cell by pinocytosis or phagocytosis. Phagosomes or pinosomes fuse
Ensuring Food Safety It is now well-known that the end product inspection and testing does not provide safety of food. It has inherent limitation of mapping the potential ha
Q. Are hormones only proteins? Some hormones are proteins, like glucagon, insulin and ADH others are derived from proteins (modified amino acids) like noradrenaline and adrenal
Processing Methods for Amelioration of Anti-Nutritional Meals from many oil seeds often contain undesirable toxic and allergenic compounds that can reduce their nutritive valu
FORMATION OF EGG MEMBRANE Ovum with egg membrane is called ova / egg. On the basis of origin the egg membrane are of three types & 1 . PRIMARY EGG MEMBRANE It is
The three charges in the figure below are at the vertices of an isosceles triangle. Let q = 7.00 nC and calculate the electric potential at the midpoint of the base.
What does acute disease mean? An sharp disease is which does not last for a longer duration n has no weakness in the body
Which book is best for quallifying in aipmt exam and what should be my minimum studying hours???
what is an ecosystem energy pathway
Q. What are the major biological functions in which chlorine ions participate? Like sodium cations, chlorine anions actively participate in the regulation of the osmolarity of
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