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Dideoxy Sequencing is the enzymatic determination and consideration of DNA or RNA sequence by the rechnique of Sanger and colleagues, based on incorporation of the chain terminating dideoxynucleotides in a growing nucleic acid strand copied by DNA polymerase or the reverse transcriptase from a DNA or RNA template. Different reactions involve dideoxynucleotides containing A, C, G, or the T bases. The reaction products represent the collection of new labeled DNA strands of the varying lengths, all terminating with the dideoxynucleotide at the 3' end (at site of a complementary base in the template nucleic acid), and are separated in the polyacrylamide or urea gel to generate the sequence "ladder". This technique is more commonly used than the "Maxam-Gilbert" (chemical) sequencing.
Photoperiodism Activity like breeding and migration in animals; flowering, seed germination in plants are regulated by the length of daily period of light and darkness. This be
Cold Hardiness and Cold Acclimation In connection with cold response we would like to introduce two terms- cold hardiness and cold acclimation. Cold-hardiness ref
Define the Water Bath with Shaker? An electric water bath with a shaker that can hold test tubes as well as conical flasks. When on a non-shake mode, it acts as a simple water
Describe Class Diplopoda and Chilopoda in detail? Members of the Subphylum Crustacea and the Subphylum Uniramia have one major characteristic in common. Both groups have biting
Define Determinants of Food Security - Food Availability? It depends on the quantum and quality of crops, livestock, fishery and other food sources, as well as commercial impor
introduction in fungai
What are the cells that form the cartilaginous tissue? The main cells of the cartilages are the chondrocytes, originate from the chondroblasts that secrete the intersticial ma
Define Nutritional Management of Food Allergies and Food intolerance? In this unit we will be discussing the adverse reactions associated with food. You may have read or heard
what is a scientific name of mango?
What is an open circulatory system? Open circulatory system is the one in which blood does not circulate only in blood vessels but it also falls in cavities that irrigate tissu
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