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Dideoxy Sequencing is the enzymatic determination and consideration of DNA or RNA sequence by the rechnique of Sanger and colleagues, based on incorporation of the chain terminating dideoxynucleotides in a growing nucleic acid strand copied by DNA polymerase or the reverse transcriptase from a DNA or RNA template. Different reactions involve dideoxynucleotides containing A, C, G, or the T bases. The reaction products represent the collection of new labeled DNA strands of the varying lengths, all terminating with the dideoxynucleotide at the 3' end (at site of a complementary base in the template nucleic acid), and are separated in the polyacrylamide or urea gel to generate the sequence "ladder". This technique is more commonly used than the "Maxam-Gilbert" (chemical) sequencing.
What are the in-vivo imaging techniques The first of the in-vivo imaging techniques, computer tomography (CT) scanning, came on stream in the early 1970s. As technologies devel
Starch gelatinization Undamaged starch granules are insoluble in cold water but can imbibe water reversibly i.e. they can swell slightly and then return to their original siz
Infectious bovine rhinotracheitis (IBR) The disease is caused by a Bovine Herpesvirus -I belonging to the family Herpesviridae and subfamily Alphaherpesvirinae. It is a DNA vi
Models for enzyme-substrate (ES) complex formation There are two popular models to explain the enzyme-substrate interaction. These are: a) Fischer's template or lock and ke
PLACENTA - A common tissue of foetus & mother (uterus) which is physical, physiological & endocrinal connection is known as placenta. FUNCTIO N - To provide nutrients
Bacterial food poisoning Food poisoning is an acute gastro-enteritis or intestinal disorder resulting from the ingestion of foods contaminated with either living microorganism
QUESTION Behaviourists underestimate the importance of biological factors such as the inherited factors, by emphasizing the simple approach of Stimulus-Response to explain beha
Q. How be able to the excretory system of annelids be described? In each segment (metamere) of the being a pair of complete excretory structures known as metanephridium exists.
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