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Dideoxy Sequencing is the enzymatic determination and consideration of DNA or RNA sequence by the rechnique of Sanger and colleagues, based on incorporation of the chain terminating dideoxynucleotides in a growing nucleic acid strand copied by DNA polymerase or the reverse transcriptase from a DNA or RNA template. Different reactions involve dideoxynucleotides containing A, C, G, or the T bases. The reaction products represent the collection of new labeled DNA strands of the varying lengths, all terminating with the dideoxynucleotide at the 3' end (at site of a complementary base in the template nucleic acid), and are separated in the polyacrylamide or urea gel to generate the sequence "ladder". This technique is more commonly used than the "Maxam-Gilbert" (chemical) sequencing.
Q. What is the major evolutionary innovation presented by nematodes? What is the benefits of that innovation? The major evolutionary innovation of nematodes is the complete dig
Explain the Biochemical Approach in Taxonomy Comparative biochemistry is being used increasingly in the systematic of animals, both for identification of organisms as well as f
What is digestion? Digestion is the breaking down of larger organic molecules obtained from the diet, e.g. carbohydrates, fats, proteins, into smaller ones, as glucose, fatty a
Triacylglycerols which is also known as triglycerides or fats consist of three fatty acid chains esterified to a glycerol backbone. Simple triacylglycerols have t
How do the availability of water and light and the climate affect the growth of a population? The availability of water and light and the climate are abiotic factors that limi
L iq uid manure handling This involves use of lot of water to flush the manure in to collection tank. Storage capacity depends on type of farm, irrigation facility, storage t
Dietitians and nutritionists Dietitians and nutritionists, who are associated with hospitals and clinics generally have regular work hours. At times, they may be required to wo
In which type of animals does the placenta exist? What is its main function? True placenta is present in placental mammals. The placenta is produced from the chorion of the
Explain Bone Healing Process in Nutritional Care? Protein is essential for callus formation, calcification and bone healing especially in cases of orthopaedic surgery. The prot
Where do the light reactions occur in prokaryotic and eukaryotic cells?
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