Dideoxy sequencing, Biology

Assignment Help:

Dideoxy Sequencing is the enzymatic determination and consideration of DNA or RNA sequence by the rechnique of Sanger and colleagues, based on incorporation of the chain terminating dideoxynucleotides in a growing nucleic acid strand copied by DNA polymerase or the reverse transcriptase from a DNA or RNA template. Different reactions involve dideoxynucleotides containing A, C, G, or the T bases. The reaction products represent the collection of new labeled DNA strands of the varying lengths, all terminating with the dideoxynucleotide at the 3' end (at site of a complementary base in the template nucleic acid), and are separated in the polyacrylamide or urea gel to generate the sequence "ladder". This technique is more commonly used than the "Maxam-Gilbert" (chemical) sequencing.


Related Discussions:- Dideoxy sequencing

Mitoses and meosis, how do mature sperm differ from those that are not full...

how do mature sperm differ from those that are not fully developed

Veterbrate, Draw and describe the structure of amphibian,reptile mammal and...

Draw and describe the structure of amphibian,reptile mammal and bird

Introduction of formation of soil, FORMATION OF SOIL Evolution of Eart...

FORMATION OF SOIL Evolution of Earth took place five to eight billion years ago when it was a red hot magma (molten rock). It took millions of years for the magma to cool down

Explain radial symmetry in classification animal kingdom, Explain Radial S...

Explain Radial Symmetry in Classification of the Animal Kingdom? All of the animals besides the sponges are considered to belong to the Eumetazoa, or the "true animals" because

Explain marine oils, Marine oils These oils typically contain large amo...

Marine oils These oils typically contain large amounts of long - chain omega-3-polyunsaturated fatty acids, with up to six double bonds and they are usually rich in vitamins A

Green leaves give off oxygen in sunlight, Green leaves give off oxygen in s...

Green leaves give off oxygen in sunlight Place some water weed under a funnel in a beaker of water reverse a test tube full of water over the tube of the funnel. Leave the appa

Parasitic flat worms, plese help for an assignment of topic parasitic flat ...

plese help for an assignment of topic parasitic flat worms for a first year zoology student.

What is deuterostome. explain in brief., What is Deuterostome. Explain in b...

What is Deuterostome. Explain in brief. Phyla, including the Chordata and Echinodermata which share common characteristics of blastopore-not forming the mouth, radial indetermi

Urine formation by excretory organs, Normal 0 false false f...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd