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Dideoxy Sequencing is the enzymatic determination and consideration of DNA or RNA sequence by the rechnique of Sanger and colleagues, based on incorporation of the chain terminating dideoxynucleotides in a growing nucleic acid strand copied by DNA polymerase or the reverse transcriptase from a DNA or RNA template. Different reactions involve dideoxynucleotides containing A, C, G, or the T bases. The reaction products represent the collection of new labeled DNA strands of the varying lengths, all terminating with the dideoxynucleotide at the 3' end (at site of a complementary base in the template nucleic acid), and are separated in the polyacrylamide or urea gel to generate the sequence "ladder". This technique is more commonly used than the "Maxam-Gilbert" (chemical) sequencing.
PHYSICAL EXAMINATION OF A CHILD This practical as you have seen above deals with assisting in various diagnostic procedures of a child. The first responsibility of you as a
Phylum Chlorophyta (green algae) 1) The chlorophyll is the main photosynthetic pigment. 2) There is little or no cell differention in thallus. Thallus is usually filamento
Q. How does aldosterone act and where is it produced? Aldosterone is a hormone that acts upon the nephron tubules stimulating the resorption of sodium Therefore it contributes
Pharynx: Pharynx is a funnel shaped tube about 13cm long that starts at the internal nares and extends to the level of the cricoid cartilage. It lies posterior to the n
Determine Nutritional Requirements in Hot Environments? Hot environments may be of two types: dry hot, as in the case of deserts or hot and humid in tropical rain forests and c
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in human and mammals how many cells result from oogenesis
How can we calculate the IVI from a total number of (30) quadrats (each 30 x 30 m quadrats)
Q. What do you mean by hemodialysis? Hemodialysis is the artificial blood filtration made by specific machines in alternative of the kidneys. Hemodialysis may be necessary in p
What is the physiological cause of the syndrome known as cretinism? Cretinism is caused by chronic deficiency of the thyroid hormones (T3 and T4) during childhood. The chronic
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