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Dideoxy Sequencing is the enzymatic determination and consideration of DNA or RNA sequence by the rechnique of Sanger and colleagues, based on incorporation of the chain terminating dideoxynucleotides in a growing nucleic acid strand copied by DNA polymerase or the reverse transcriptase from a DNA or RNA template. Different reactions involve dideoxynucleotides containing A, C, G, or the T bases. The reaction products represent the collection of new labeled DNA strands of the varying lengths, all terminating with the dideoxynucleotide at the 3' end (at site of a complementary base in the template nucleic acid), and are separated in the polyacrylamide or urea gel to generate the sequence "ladder". This technique is more commonly used than the "Maxam-Gilbert" (chemical) sequencing.
mode of digestion in coelenterata
How are secondary cell walls different from primary cell walls? Primary cell walls are assembled on the surface of the plasma membrane whereas the cell is growing. They can gro
Transfer of pollen grains from the anther to the stigma of another flower of the similar plant is known as? Geitonogamy
Carbohydrates and proteins question #Minimum 100 words accepted#
The modern water treatment plan is often called upon to perform wonders with the water fed to it. The clear, safe , even tasteful water that comes from a faucet may have started a
Q. What are the antigen-presenting cells of the immune system? The antigen-presenting cells of the immune system also known as APC cells are cells that do digestion and phagocy
wHAT IS 10um?
how mollusca bore into hard substrate
what are disadvantage of protozoa
Which of the following serves as an actuating signal, or as part of an actuating signal, in a negative feedback system? A. Action potentials in diaphragm skeletal muscle fibers
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