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Dideoxy Sequencing is the enzymatic determination and consideration of DNA or RNA sequence by the rechnique of Sanger and colleagues, based on incorporation of the chain terminating dideoxynucleotides in a growing nucleic acid strand copied by DNA polymerase or the reverse transcriptase from a DNA or RNA template. Different reactions involve dideoxynucleotides containing A, C, G, or the T bases. The reaction products represent the collection of new labeled DNA strands of the varying lengths, all terminating with the dideoxynucleotide at the 3' end (at site of a complementary base in the template nucleic acid), and are separated in the polyacrylamide or urea gel to generate the sequence "ladder". This technique is more commonly used than the "Maxam-Gilbert" (chemical) sequencing.
Why are heterotrophic, autotrophic , and mixotrophic protists not classified as plants or animals ?
do fats with one or more 'kinky' tail fatty acids tend to be solid or liquid at room temperature? These are found in triglycerides forming what? Solid fats or oils? Is it opposite
It is IgG mediated cytotoxic hypersensitivity. Typical manifestations contain erythroblastosis fetalis, hemolytic anemia, blood transfusion reactions etc.
what are some of the human needs??
Define Food Intake during Polar Expeditions? Observations made by Easty (1967) at Halley Bay, the British Antarctic survey base during 1961-62 expeditions indicate mean calorie
Define Glass photo-emissive tubes? Glass photoemissive tubes are those which consist of a curved metal cathode, coated with a photosensitive material. While light strikes its s
How are glycosidic bonds formed? The anomeric hydroxyl group and a hydroxyl group of another sugar or some other compound can join together, splitting out water to form a glyco
Problem 1: Describe the functions of: a. Glutathione transferases b. Sulfotransferases. -Describe the functions of: a. Glutathione transferases Describe the functions of
Define Use of Soy Protein Concentrates in Meat products? This area probably represents the most important application of soy protein concentrates (SPC) in the food industry. SP
Why are trees green? It is because leaves have chloroplasts, which have a green pigment, which also have chlorophyll in them. They are used for conducting photosynthesis, which
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