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Dideoxy Sequencing is the enzymatic determination and consideration of DNA or RNA sequence by the rechnique of Sanger and colleagues, based on incorporation of the chain terminating dideoxynucleotides in a growing nucleic acid strand copied by DNA polymerase or the reverse transcriptase from a DNA or RNA template. Different reactions involve dideoxynucleotides containing A, C, G, or the T bases. The reaction products represent the collection of new labeled DNA strands of the varying lengths, all terminating with the dideoxynucleotide at the 3' end (at site of a complementary base in the template nucleic acid), and are separated in the polyacrylamide or urea gel to generate the sequence "ladder". This technique is more commonly used than the "Maxam-Gilbert" (chemical) sequencing.
A species has a diploid chromosome number of 56. Is it true or false that of the 28 chromosomes in an ovum, 14 are always paternal? Ignore the effects of crossing over. Justify you
Explain Cardiac disease Cardiac conduction abnormalities associated with Lyme disease are generally self-limited. Patients with minor cardiac involvement (first-degree atrioven
Explain the Importance of Vitamin E Vitamin E has a pronounced antioxidizing effect. Because of this property, vitamin E decreases the basic metabolism of the tissues or the ox
A cell in G1 of interphase has 12 chromosomes. How many chromosomes and DNA molecules will be found per cell when this original cell progresses to the G2?
Hypomagnesaemic tetany (grass tetany, grass staggers, lactation tetany) Hypomagnesaemic tetany is a multifactorial complex metabolic disorder, which is characterized by reduct
What is the stage of the cardiac cycle during which the ventricles are filled? The filling of the ventricles with blood happens during diastole. The Circulatory System - Ima
Patients Not on HAART For HIV-infected patients requiring TB treatment who are not currently being treated with highly active antiretroviral therapy (HAART), it may be prudent
The capacity to do work is known as energy. In an ecosystem energy flows through one organism to another in the form of food. The flow of energy is uni-direction and non cycle and
Does radioactive dating with isotopes of uranium and thorium provide an estimate of the starting, middle, or end of the period of Earth's formation? Explain your answer No, th
Q. What is the neuromuscular synapse? Neuromuscular synapse is the structure through which the neural impulse passes from the axon of a motor neuron to the muscle cell. This st
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