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Dideoxy Sequencing is the enzymatic determination and consideration of DNA or RNA sequence by the rechnique of Sanger and colleagues, based on incorporation of the chain terminating dideoxynucleotides in a growing nucleic acid strand copied by DNA polymerase or the reverse transcriptase from a DNA or RNA template. Different reactions involve dideoxynucleotides containing A, C, G, or the T bases. The reaction products represent the collection of new labeled DNA strands of the varying lengths, all terminating with the dideoxynucleotide at the 3' end (at site of a complementary base in the template nucleic acid), and are separated in the polyacrylamide or urea gel to generate the sequence "ladder". This technique is more commonly used than the "Maxam-Gilbert" (chemical) sequencing.
Endothelium is a source of vasoconstrictor factor also. The best characterized of these are the endothelins. Endothelium produces only ET1. Unlike NO, which is released rapidly in
Q. How is excretion done in fishes? Fishes have a pair of kidneys that filtrate the blood, bony fishes excrete nitrogen as ammonia NH3 (they are ammoniotelic) and cartilaginous
Q. What do you mean by Subxiphoid Long Axis Sweep? This sweep begins with keeping the transducer at subxiphoid region, positioning directly posterior with marker pointing towar
1. In modern blimps, the gas of choice used to inflate them is helium rather than hydrogen. Hydrogen would be lighter, but helium is safer. Compare and contrast the atomic structur
Q. Counselling skills used in diabetes mellitus? Information gathering is an important skill for understanding of the client. Behaviour is complex and, therefore it is importan
Peptide bond formation is catalyzed by peptidyl transferase. The carboxyl end of the amino acid bound to the tRNA in this reaction and in the P site is uncoupled from the tRNA a
What are restriction enzymes? How do these enzymes participate in the recombinant DNA technology? The Restriction enzymes, or restriction endonucleases, are enzymes specialized
How many protein require patient in a day Patients who require high protein diets are encouraged to drink 600-800 mL of milk a day. Now, you must be wondering how one can consu
F a t cow syndrome Fat cow syndrome, also known as fatty infiltration of liver, is a highly fatal disease in high yielding dairy animals that occurs a few days before or afte
Use of trichomoniasis in pregnancy Trichomoniasis in pregnancy has been associated with adverse pregnancy outcomes (D Soper, Am J Obstet Gynecol 2004; 190:281). Metronidazole
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