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Dideoxy Sequencing is the enzymatic determination and consideration of DNA or RNA sequence by the rechnique of Sanger and colleagues, based on incorporation of the chain terminating dideoxynucleotides in a growing nucleic acid strand copied by DNA polymerase or the reverse transcriptase from a DNA or RNA template. Different reactions involve dideoxynucleotides containing A, C, G, or the T bases. The reaction products represent the collection of new labeled DNA strands of the varying lengths, all terminating with the dideoxynucleotide at the 3' end (at site of a complementary base in the template nucleic acid), and are separated in the polyacrylamide or urea gel to generate the sequence "ladder". This technique is more commonly used than the "Maxam-Gilbert" (chemical) sequencing.
Temperate rain forests - Ecosystem The temperate rain Forests are colder than any other rain forest and exhibit a marked seasonality with regard to temperature and rainfall. R
Define the Food and Nutrition Security? In the previous section, we learnt about the trends in production of food grains in the country and their relationship to population gro
List and briefly describe the mechanisms that prevent acid-base imbalances from appearing in the body. In addition, indicate where each mechanism functions most significantly.
How does vitamin C act in the body? What is the harm caused by insufficiency of vitamin C? Why was this deficiency also known as "sailors' disease"? Vitamin C, or ascorbic acid
lichens
Primary Egg Envelopes These are the egg envelopes which develop in the ovary between oocyte and follicle cells in the space occupied by the interdigitating microvilli. Such e
Explain Regulatory enzymes Regulatory enzymes: Citrate synthase, isocitrate dehydrogenase and a-ketoglutarate dehydrogenase complex are the key enzymes which regulate
Q What are halophile, methanogen archaebacteria and thermoacidophile? There are three peculiar types of archaebacteria the halophile archaebacteria only survive in salt-rich en
Economic Status Economic Status : It is one of the important practical considerations to be kept in mind while formulating a diet prescription. During an acute illness, a
What is Implant Failure The total failure of the implant to fulfill its purpose which are functional, esthetic and phonetic because of mechanical or biologic reasons.
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