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Dideoxy Sequencing is the enzymatic determination and consideration of DNA or RNA sequence by the rechnique of Sanger and colleagues, based on incorporation of the chain terminating dideoxynucleotides in a growing nucleic acid strand copied by DNA polymerase or the reverse transcriptase from a DNA or RNA template. Different reactions involve dideoxynucleotides containing A, C, G, or the T bases. The reaction products represent the collection of new labeled DNA strands of the varying lengths, all terminating with the dideoxynucleotide at the 3' end (at site of a complementary base in the template nucleic acid), and are separated in the polyacrylamide or urea gel to generate the sequence "ladder". This technique is more commonly used than the "Maxam-Gilbert" (chemical) sequencing.
Q. What do you mean by Linnaeus binomial system? Almost a century after Bauhin the great Swiss naturalist Carolus Linnaeus (1707-1778) published the two monumental works the "S
Hydrolyases These enzymes (code EC 3) catalyze the hydrolytic cleavage of C-O, C-N, C-C and some other bonds, including phosphoric anhydride bonds. Their trivial names are f
Define Influence of Gardening on the Development of Botany? The influence of gardening on the development of botany may be seen in the work of John Gerard (1545-1612). Gerard p
Q. How can you describe Pleural Effusion? Fluid has a density indistinguishable from soft tissue on a radiograph. Pleural fluid tends to accumulate in the deepest part of the
how is water lost from respiratory surface
Give definitions of atomic number and atomic mass and how they relate to protons, neutrons, and electrons in neutral atoms?
What are the cells that produce the myelin sheath? Of which substance is the myelin sheath formed? In the central nervous system (CNS) the myelin sheath is made by apposition o
Compare and contrast enantiomers, epimers, and anomers, specifically in relation to sugars.
Q. What functions regulates by hypothalamus? HYPOTHALAMUS - Main visceral control center of the body (yet small in size). Central to virtually all homeostatic control . Th
Q. What are the Chronic complications of diabetes? • Atherosclerosis: Degeneration of walls of the arteries due to fatty plaques - deposition on arterial walls. Diabetics ar
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