Determine the sucrose content in a sample of honey, Biology

Assignment Help:

Q. Determine the sucrose content in a sample of honey?

This activity will help you to:

• estimate the non-reducing sugars and total sugars in a sample of honey,
• check the conformance or non-conformance of the samples to the standards, and
• detect adulteration of honey with commercial sugars.

Reagents Required

1) Standard invert sugar solution: Weigh accurately 0.985 g of sucrose and dissolve in 500 ml of water. Add 2 ml of conc. H2SO4. Boil gently for 30 minutes and keep aside for 24 hours. Neutralize this with Na2CO3 and make the final volume to 1000 ml. 30 ml of this solution contains 0.05 g of invert sugar.

2) Fehling A and Fehling B

3) Methylene blue indicator.

4) 0.4 N HCl

5) 0.6 N NaOH

Materials Required

Burette

Pipette

Conical flask

Beaker

Distilled water


Related Discussions:- Determine the sucrose content in a sample of honey

Ringworm, R i n g w o r m It is also known as dermatophytosis re...

R i n g w o r m It is also known as dermatophytosis resulting in alopecia, scab formation and ring like lesions in the affected body parts. Eti o logy: Zeop

Steps to follow in diagnosis of dysentry, Assessment   The child with dy...

Assessment   The child with dysentery will present you with sudden onset of fever, prostration, vomiting, bloody diarrhoea, abdominal pain and tenesmus. Patient may suffer from

Historical development of psychiatric nursing, HISTORICAL DEVELOPMENT OF PS...

HISTORICAL DEVELOPMENT OF PSYCHIATRIC NURSING: History is a  record of opportunities, recognized and informed,  that produce profound changes in a given field. History of psyc

Phases of nurse-patient relationship, PHASES OF NURSE-PATIENT RELATIONSHIP:...

PHASES OF NURSE-PATIENT RELATIONSHIP: Kapoor, Bimla (1994) has listed four phases of  nurse patient relationship which are explained below while describing the phases she has

What are the major parts of ferns, Q. What are the major parts of ferns? ...

Q. What are the major parts of ferns? Ferns are constituted by small roots that come downwards from the rhizome stem and often horizontalized. The fronds also arise from the rh

How are the antibodies against the rh factor formed, How are the antibodies...

How are the antibodies against the Rh factor formed? The Anti-Rh antibodies are made by humoral immune response. When the Rh- individual makes contact with the Rh factor this i

Define important food sources of protein, Define Important Food Sources of ...

Define Important Food Sources of Protein? The important sources of proteins in the diets of low-income groups are cereals and legumes. Meat, fish, eggs and milk are important s

Explain techniques for broken instrument removal, Explain Techniques for Br...

Explain Techniques for Broken Instrument Removal Described by Gary Carr Staging platform (cutting in flat surface) a) Create straight line access to separated file using mod

When manifested in homozygosity in the offspring?, Why can it be said that ...

Why can it be said that a recessive allele can remain hidden in the phenotype of an individual and revealed only when manifested in homozygosity in the offspring? A recessive a

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd