Determine the procedure of rothera test, Biology

Assignment Help:

Rothera's Test

It is highly sensitive for both acetoacetic and acetone. Although it is one of the oldest methods still it is widely used.

Procedure:

- Take about 5ml of urine in a test tube and saturate with ammonium sulphate and add few crystals (or about 0.5ml of 20% aqueous solution) of sodium nitroprusside. If solid crystals are taken, shake well the tube to dissolve the crystals and wait for about 10 minutes.

- Now carefully pour down the side of the test tube about 2 - 3 ml of concentrated ammonia (Liquor ammonia) so that it forms a layer on the top of saturated urine. Formation of a purple ring at the junction indicates positive test.

 


Related Discussions:- Determine the procedure of rothera test

Cell, How cell divides?

How cell divides?

Explain the kidney function in human biology, Explain the Kidney Function i...

Explain the Kidney Function in human biology? Blood first enters the capillaries in Bowman's capsule where it is filtered. The pores in the capillary walls allow water and smal

Development and differentiation of a flowering plant, Development and Diffe...

Development and Differentiation of a flowering plant The developmental phases of a flowering plant involve seed germination, vegetative growth, flowering, fruiting and senesce

What is the difference between phenotype and genotype, What is genotype? Wh...

What is genotype? What is the difference between phenotype and genotype? The Genotype is the genes, DNA nucleotide sequences contained in the chromosomes of an individual, whic

Biological evolution, Natural selection is a process which directs all biol...

Natural selection is a process which directs all biological evolution. It is a process that directs genetic changes which when proved to be adapted to the environment are retaine

How auxins act helping the lateral growth of the stem, How auxins act helpi...

How auxins act helping the lateral (secondary) growth of the stem? The Auxins stimulate the formation of conductive vessels, phloem and xylem, promoting the thickening of the s

What is the cyclosis, Q What is the cyclosis? Cyclosis is a kind of int...

Q What is the cyclosis? Cyclosis is a kind of interior cell movement in which an oriented flow of circulating material is created and maintained in the cytoplasm by the action

Virus, #question.bacteria and virus are meet so what happen.

#question.bacteria and virus are meet so what happen.

Statistical investigation, Meaning of Statistical Investigation: An inv...

Meaning of Statistical Investigation: An investigation means a systematic search for the knowledge or the truth. In statistical investigation the search for the knowledge is do

Available and non-available water, Available and Non-available Water Yo...

Available and Non-available Water You have seen above that water is present even in the apparently dry soils but it is of not much use as far as the plants are concerned. They

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd