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For many years, students who have occupied my Molecular Biology course have now applied for lab tech jobs exact after graduation. But I have NEVER had a student fail to obtain a job before graduation. One purpose for this is that my all students learn to create solutions. Four problems are here which you could remain off the unemployed college graduate list.
a) You obtain an optical density reading of a DNA sample but this is too concentrated to provide you an accurate reading. You obtain 1 μL of the DNA sample and dilute this with 14 μL of dH2O. You after that take a reading of the DNA onto the spectrophotometer and this provide you an OD reading of 0.123. As written above the spectrophotometer is such above conversion factor: 1 OD of ds DNA = 50 μg/mL of DNA.
Question: Determine the concentration of your original DNA sample?
b) You require preparing a few solutions for a Southern blot. You are provided 50X SSC, 10 percent SDS as well as 5 M NaOH.
Question: Explain how would you make 250 mL of a solution those final concentrations were 1X SSC, 0.25 percent w/v SDS and 10 mM NaOH? When FW of NaOH is 40.0.
Q. What is the endosymbiotic hypothesis about the origin of mitochondria? And what are the molecular facts that support the hypothesis? And To which other cellular organelles can t
List the reactions that need to be circumvented by the special reactions in gluconeogenesis. The reactions that are circumvented include: Between pyruvate and phosphoenolpy
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A decrease in parasympathetic discharge to the heart leads to A. an increase in the conductance of F-channels in SA node cells. B. a decrease in the conductance of potassium
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What is Ceramics A kind of material that is "bone like" based on the specific ratios of calcium and phosphorous in particular crystalline structures. Ceramics are inorganic, no
What is linkage? Two genes are assumed to be under linkage, or linked, when they reside in the same chromosome. For instance, the research of the human genome discovered tha
How is the cerebrum anatomically divided? The cerebrum is divided into two cerebral hemispheres, the right and the left. Each hemisphere is made of four cerebral lobes: frontal
During a routine annual physical, a patient was checked to determine the amount of glucose in the blood. After the assay, It was found that the glucose that the glucose concentrati
Q. What are the examples of secretory cells? Endocrine and exocrine pancreatic cells, parathyroid and thyroid endocrine cells, adenohypophysis, adrenal and pineal endocrine cel
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