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For many years, students who have occupied my Molecular Biology course have now applied for lab tech jobs exact after graduation. But I have NEVER had a student fail to obtain a job before graduation. One purpose for this is that my all students learn to create solutions. Four problems are here which you could remain off the unemployed college graduate list.
a) You obtain an optical density reading of a DNA sample but this is too concentrated to provide you an accurate reading. You obtain 1 μL of the DNA sample and dilute this with 14 μL of dH2O. You after that take a reading of the DNA onto the spectrophotometer and this provide you an OD reading of 0.123. As written above the spectrophotometer is such above conversion factor: 1 OD of ds DNA = 50 μg/mL of DNA.
Question: Determine the concentration of your original DNA sample?
b) You require preparing a few solutions for a Southern blot. You are provided 50X SSC, 10 percent SDS as well as 5 M NaOH.
Question: Explain how would you make 250 mL of a solution those final concentrations were 1X SSC, 0.25 percent w/v SDS and 10 mM NaOH? When FW of NaOH is 40.0.
describe the diversity in the feeding habits and locomotion of annelids
What are gibberellins? Where are they produced? Gibberellins are plant hormones that stimulate plant growth, flowering and fruit formation (also parthenocarpy) and the germina
What happens when the cell membrane or plasma membrane ruptures or breaks down? When cell membrane ruptures Ions leek out and if not repaired in time the cell will die. As we k
Assume for this question that we are discussing a rare human disorder. Describe as detailed as possible the characteristics of this disorder if it is: autosomal dominant autosomal
What is the relationship between the estrogen level and the LH level in the menstrual cycle? What is the function of LH in the menstrual cycle and when does its blood concentration
If a cow develops a preference for eating white four o'clock flowers and ignoring pink and red four o'clock flowers, what type of selection is being demonstrated? Would the cow eve
This process involves the regulation of the concentration of the body fluids through control of water content and salt content of the blood. If a person loses water from their bloo
Japanese encephalitis The Japanese encephalitis is a mosquito-born flavivirus infection that causes encephalitis in human and equines and abortion in pigs, caused by Japanese
how temperature acts as limiting factor explain?
STRUCTUR E OF A MYOFIBRIL - The dark bands of the myofibril are termed the A-bands (Anisotropic bands). Each A-band has at its middle a light zone called H-zone (Henson'
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