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For many years, students who have occupied my Molecular Biology course have now applied for lab tech jobs exact after graduation. But I have NEVER had a student fail to obtain a job before graduation. One purpose for this is that my all students learn to create solutions. Four problems are here which you could remain off the unemployed college graduate list.
a) You obtain an optical density reading of a DNA sample but this is too concentrated to provide you an accurate reading. You obtain 1 μL of the DNA sample and dilute this with 14 μL of dH2O. You after that take a reading of the DNA onto the spectrophotometer and this provide you an OD reading of 0.123. As written above the spectrophotometer is such above conversion factor: 1 OD of ds DNA = 50 μg/mL of DNA.
Question: Determine the concentration of your original DNA sample?
b) You require preparing a few solutions for a Southern blot. You are provided 50X SSC, 10 percent SDS as well as 5 M NaOH.
Question: Explain how would you make 250 mL of a solution those final concentrations were 1X SSC, 0.25 percent w/v SDS and 10 mM NaOH? When FW of NaOH is 40.0.
Mycotic diseases Mycotic (fungal) diseases of animals and birds are of considerable importance both from public health and economic point of view. The fungi responsible for an
The holoenzyme is denatured by heat at very high temperature. It is found that two polypeptides, although denatured, contains chains that remain together as a unit, while two other
Define Precautions for Determination of Inorganic Phosphorus? 1. All reagents should be added in the order mentioned. 2. Allow 10 minutes for the colour to develop in each t
taxonomy research cat
Some relatively simple eukaryotes, like the adder's tongue fern, may have many more chromosomes than a more complex eukaryote, like a mammal. What might this suggest about the size
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The initial stage of packaging have the binding of the chromosomal DNA to histones. Whole, in chromosomes the ratio of the DNA to histones on a weight basis is around 1:1. There a
Question 1 Discuss how you would perform mounting of stained sections. List commonly used mounting media in histopathology laboratory. Add a note on advantages and disadvantages o
Soil-Environmental Components The word soil is derived from Latin word 'solum' meaning earthy material in which plants grow. The soil is the consolidated outer layer of the ear
Oxaloacetate has two main roles. It is an intermediate which is consumed in gluconeogenesis and it is also a key intermediate in the citric acid cycle where it fuses with acetyl Co
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