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For many years, students who have occupied my Molecular Biology course have now applied for lab tech jobs exact after graduation. But I have NEVER had a student fail to obtain a job before graduation. One purpose for this is that my all students learn to create solutions. Four problems are here which you could remain off the unemployed college graduate list.
a) You obtain an optical density reading of a DNA sample but this is too concentrated to provide you an accurate reading. You obtain 1 μL of the DNA sample and dilute this with 14 μL of dH2O. You after that take a reading of the DNA onto the spectrophotometer and this provide you an OD reading of 0.123. As written above the spectrophotometer is such above conversion factor: 1 OD of ds DNA = 50 μg/mL of DNA.
Question: Determine the concentration of your original DNA sample?
b) You require preparing a few solutions for a Southern blot. You are provided 50X SSC, 10 percent SDS as well as 5 M NaOH.
Question: Explain how would you make 250 mL of a solution those final concentrations were 1X SSC, 0.25 percent w/v SDS and 10 mM NaOH? When FW of NaOH is 40.0.
how are the listed from largest group to smallest group
Define Carbohydrate requirement to avoid underweight problem? Liberal amounts of easy to digest carbohydrates should be included in the diet. The intake of dietary fibre should
Q. What are the effects of somatostatin for the pancreatic hormonal secretion? Somatostatin inhibits both glucagon and insulin secretions.
International support to control HPAI and other TADs In 2008, a new global approach called "One World, One Health" was defined by the four international technical agencies, i.e
During mitosis ER and nucleolus begin to disappear at: 1. Late prophase 2. Early metaphase 3. Late metaphase 4. Early prophase Late prophase
What is biotechnology? Biotechnology is the application of biological knowledge to get new techniques, materials and compounds of pharmaceutical, medical, agrarian, industrial
What are synthetic auxins and what are their uses? Synthetic auxins, like indolebutyric acid (IBA) and naphthalenic acid (NAA) are substances same to IAA (a natural auxin) but
Determine the Nature and Degree of Abnormality The nature and degree of abnormality will effect normal neural growth and maturational outcome. For example, in an adult damage t
What is a goiter? What is endemic goiter? How is this problem socially solved? Goiter is the abnormal enlargement of the thyroid gland. The goiter appears as a tumor in the ant
In the early 1970's, Schroeder and his group designed a single stage implant system known by the name of ITI Implant System. They demonstrated that one-stage implant can also resul
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