Determine reagents for determination of protein content, Biology

Assignment Help:

Determine Reagents for Determination of Protein Content Using Biuret Method?

Collect the following reagents to conduct the experiment:

1. 0.2 N NaOH solution - Dissolve 8 g of NaOH pellets in 1 litre of distilled water.

2. Biuret reagent - Dissolve 90 g of Rochelle salt (sodium potassium tartarate) in 500 ml of 0.2 N sodium hydroxide solution. Add 10 g of copper sulphate and 10 g KI and volume is made to 2 L with 0.2 N alkali.

3. Working standard - Dissolve 200 mg albumin in 100 ml distilled water (2 mg/ml).


Related Discussions:- Determine reagents for determination of protein content

Biological responses controlled by phytochrome, Biological Responses Contro...

Biological Responses Controlled by Phytochrome Phytochrome responses are those which are controlled reversibly by red and far-red light. These can be broadly categorised as:

What are the signs of cardiac tamponade, Q. What are the Signs of cardiac t...

Q. What are the Signs of cardiac tamponade? The clinical presentation will be that of a low output state with anxiety, restlessness, dyspnoea, sweating, cold extremities and dr

Human or mammalian heart, HUMA N HEART (MAMMALIAN HEART) - Position -...

HUMA N HEART (MAMMALIAN HEART) - Position - Situated in thoracic cavity in pericardial cavity close to its front wall. Its broad base faces upward and backward. Its narrow a

Tricuspid annuloplasty-types of surgery, Tricuspid Annuloplasty :  Tr...

Tricuspid Annuloplasty :  Tricuspid valve is evaluated by digital palpation before connecting the patient to cardio pulmonary by pass. Functional regurgitation can be repaire

Cell theory to be developed after microscopes, Why did it take 150 years fo...

Why did it take 150 years for the cell theory to be developed after microscopes were invented? Information about cells could not be understood and organized into a central theo

What are dna ligases, What are DNA ligases? How do these enzymes participat...

What are DNA ligases? How do these enzymes participate in the recombinant DNA technology? DNA ligases are enzymes specialized in tying the complementary DNA chains that produce

Historically what were the two main evolutionary theories, Historically wha...

Historically what were the two main evolutionary theories? The two major evolutionary theories were darwinism and lamarckism.

Chemolithoautotrophs - nutritional types of microorganisms, Explain Chemoli...

Explain Chemolithoautotrophs - Nutritional Types of Microorganisms These microorganisms use inorganic compound as source of energy and electron and CO 2 as a source of carbon.

Adhesion of cells to extracellular cell matrix, Adhesion of Cells to Extrac...

Adhesion of Cells to Extracellular Cell Matrix You know that the cells in order to move or change shape, adhere to each other or to the substratum in their environment. Usual

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd