Determine reagents for determination of protein content, Biology

Assignment Help:

Determine Reagents for Determination of Protein Content Using Biuret Method?

Collect the following reagents to conduct the experiment:

1. 0.2 N NaOH solution - Dissolve 8 g of NaOH pellets in 1 litre of distilled water.

2. Biuret reagent - Dissolve 90 g of Rochelle salt (sodium potassium tartarate) in 500 ml of 0.2 N sodium hydroxide solution. Add 10 g of copper sulphate and 10 g KI and volume is made to 2 L with 0.2 N alkali.

3. Working standard - Dissolve 200 mg albumin in 100 ml distilled water (2 mg/ml).


Related Discussions:- Determine reagents for determination of protein content

Explain about oil-bearing fruits, Explain about Oil-bearing fruits Oil-...

Explain about Oil-bearing fruits Oil-bearing fruits, nuts and seeds have been grown and used for food for many centuries. More than 100 varieties of plants are known to have oi

Determine the principles of suturing, Determine the Principles of suturing ...

Determine the Principles of suturing These are universal principles which are applicable in all aspects not just implant surgery: 1. Sutures should always be inserted throug

Explain mitosis properly, Q. Does the mitosis properly occur after or befor...

Q. Does the mitosis properly occur after or before the interphase? Is it a simple "point of view" issue? Mitosis have to be considered a succeeding phase after interphase since

What are plankton, What are plankton, benthos and nekton? Plankton, ben...

What are plankton, benthos and nekton? Plankton, benthos and nekton are the three groups into which aquatic living beings may be divided. The plankton is formed by algae and

Liver function test, Liver function test should be done for all diabetic pa...

Liver function test should be done for all diabetic patients. This will help the doctor in the choice of drugs for the treatment of diabetes since liver abnormalities influence the

List the requirements of implant materials, List the requirements of implan...

List the requirements of implant materials. a) Biologically compatibility: an ideal implant material will elicit mainly physiological reactions within the surrounding tissues (

Define the visual accommodation, Q. What is visual accommodation? Visua...

Q. What is visual accommodation? Visual accommodation is the phenomenon of varying the curvature of the crystalline lens to make possible the difference of its refractivity to

Explain phylum phytofagellata, Phylum Phytofagellata (Plant flagellates...

Phylum Phytofagellata (Plant flagellates) 1) At some stage of life cycle they have one or more flagella. The flagella are used for locomotion. 2) Photosynthetic protistan

Platelet, What happens if platelets aren''t present in blood?

What happens if platelets aren''t present in blood?

Determine possible polypeptides, a) The image above is part of a DNA sequen...

a) The image above is part of a DNA sequencing gel. Assuming this is the DNA coding strand and you are reading 5' -> 3' what are ALL the possible polypeptides this sequence cou

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd