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a) The image above is part of a DNA sequencing gel. Assuming this is the DNA coding strand and you are reading 5' -> 3' what are ALL the possible polypeptides this sequence could code for?
b) If this sequence is located within an exon of an actively transcribed gene, which ONE of the possible polypeptide sequences must be the correct one and why?
c) Starting with the correct polypeptide sequence, what will be the new polypeptide sequence if:
(i) the thymine at position 10 is replaced by guanine?
(ii) the adenine at position 15 is replaced by cytosine?
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working of pcr
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