Determination of analyte concentration, Physics

Assignment Help:

Determination of Analyte Concentration:

One of the most common applications of spectrophotometry is to determine the concentration of an analyte in a solution. There is a relationship between concentration and absorbance.  This relationship is expressed by the Lambert-Beer law, which is more commonly known as Beer's law.  This law states that the absorbance of a light absorbing material is proportional to its concentration in solution.

A = Ebc

E = the extinction coefficient of the substance, has units of M-i  * cm-i  (unique for each substance)

b = the sample path length measured in centemeters (i.e. the width of the cuvette-almost always i cm)

c = the molar concentration of the solution (you must express concentration in terms of molarity)

It is because of this relationship that biologists measure absorption rather than transmission. The Lambert-Beer law can be used to calculate the concentration of a solution if its extinction coefficient is known.  To determine the extinction coefficient, you measure the absorbance of a known concentration of solution and then rearrange the equation to solve for E.=

Table.  Visible Spectrum and Complementary Colors

1471_Determination of Analyte Concentration.png

 


Related Discussions:- Determination of analyte concentration

Illustrate the term temperature sensor, Illustrate the term temperature sen...

Illustrate the term temperature sensor? Temperature sensor: Bimetal coated onto the tip of fiber. When temperature increases the bimetal bends because of difference within t

Discuss short note on galvanometer, It is an instrument used to detect smal...

It is an instrument used to detect small current passing by it by showing deflection. Galvanometers are of dissimilar types e.g. moving coil galvanometer, moving magnet galvanomete

Describe what is one beat , If the intensity of sound is maximum at time t ...

If the intensity of sound is maximum at time t = 0, one beat is known as formed when intensity changes into maximum again after becoming minimum once in among.

Largest acceptable prostate volume cubic centimeters, Prof. Patch is collab...

Prof. Patch is collaborating with physicians at MCW on an ex vivo prostate cancer imaging trial. The testbed they currently use is 6 cm wide. The research nurse coordinating the

Mechanics, what is reduce mass in two mass system?

what is reduce mass in two mass system?

Presbyopia, Presbyopia: The accommodation power of a person is lost by ...

Presbyopia: The accommodation power of a person is lost by which the suffers a long sightedness. It is known as Presbyopia or lack of accommodation.

Tesla, Tesla; T (after N. Tesla, 1870-1943): The derived SI unit of ma...

Tesla; T (after N. Tesla, 1870-1943): The derived SI unit of magnetic flux density, described the magnetic flux density of magnetic flux of 1 Wb by an area of 1 m 2 ; thus it

Evaluate velocity of particle b relative to a, In laboratory frame of refer...

In laboratory frame of reference, particle A moves along X direction with velocity 0.5 c and particle B moves along Y direction with velocity 0.4 c. Evaluate velocity of particle B

Phosphorus cycle, How does electricity affect the phosphorus cycle?

How does electricity affect the phosphorus cycle?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd