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Describe DNA replication in details?
Replication : DNA replicates itself by first breaking the hydrogen bonds between the nitrogen base pairs, and "unzips" itself into two strands. A replication fork, a Y-shaped structure, moves down the strands as DNA unzips.
Complementary nucleotides, which are floating free in the nucleus, form hydrogen bonds with each separated DNA strand at their matching nucleotide sites according to the base-pairing rule. In this way,
DNA replication is a semi-conservative process whereby each half of the original DNA strand builds a new complementary strand on itself. DNA polymerases catalyze the formation of sugar to phosphate bonds of the nucleotide monomers to complete the building of a new strand on the original strands.
Replication takes place at a very fast rate. In the bacterium E. coli, the complex makes DNA at over 1000 base pairs per second, and makes mistakes in the order of perhaps one base in a billion to one per trillion. In bacteria, there is just one point where replication begins, but in eukaryotes there are many specific origins for replication. Replication can proceed in both directions from an origin.
Preparation and properties of modified starches Improved performance of starches may be realized by physical and chemical modifications. The major physically modified starch i
A restriction map of plasmid and a human gene of identified sequence is demonstrated below. Your job is to clone the human liver cDNA for that gene than the start codon is closer t
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If a codon is CDC, what proportion of nucleotide changes in the third position result in a new amino acid? If the codon is AGG, what proportion of changes in the first position res
how does a cell work ?
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Assume that you have 1 mL of a solution amylase (an enzyme) at a concentration of 15 mg protein/mL. Calculate the volume of diluting buffer that you would have to add to 1.0 mL of
Regulation of HMP Pathway The following factors play an important role in regulation of HMP pathway: a) The first reaction of this pathway catalysed by glucose-6-phosphate
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